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The map label for this gene is ligA [H]

Identifier: 66045063

GI number: 66045063

Start: 2097011

End: 2099374

Strand: Direct

Name: ligA [H]

Synonym: Psyr_1819

Alternate gene names: 66045063

Gene position: 2097011-2099374 (Clockwise)

Preceding gene: 66045062

Following gene: 66045064

Centisome position: 34.41

GC content: 62.56

Gene sequence:

>2364_bases
ATGAAGGCCGTCGAAACCCGAATCCTGGAACTGCGTGCAGAGCTGGATCAGCACAACTACCGCTACCACGTTCTGGATGA
ACCGAGCATTCCGGATGTCGAGTACGACCGCCTGTTTCACGAGCTCAAGGCCCTGGAGGCGGAAAACCCCCATCTGGTCA
CCCCTGATTCGCCGACGCAGCGGGTCGGAAGCGCGGCCTTGTCGGCGTTCACCCAGGTGCGTCACGAAATGCCGATGCTC
AGCCTGGGCAACGCGTTCGAAGAAAACGACATGCTCGAATTCGATCGGCGGGTCACCGAGGGGCTGGATCTGCCGGCCGG
GGACCTGTTTGGCGAAGGCGGCAAGGTGCAGTACAGCTGCGAGCCCAAGCTGGACGGTCTGGCCGTCAGCCTGCTGTATC
GCGACGGTGCGCTGGTGCGGGGCGCGACACGCGGCGACGGAACCACCGGCGAAGACATCAGTGTCAACGTGCGCACAGTA
CGCAACATCCCGCTCAAGCTGCAGGGCAAGGGCTGGCCGGATGTGCTGGAAGTGCGCGGTGAAGTGTTCATGTCCAAGGC
CGGTTTCGAGCGGCTTAACGCCAGCCAGCTGGAAATCGGCGGCAAGACCTTCGCCAACCCGCGCAACGCAGCCGCAGGCA
GCCTGCGTCAGCTGGATTCCAAGATCACGGCCAACCGGCCGCTGGAGTTCTGCTGCTATGGTCTGGGCCAGACGTCGGCG
GAAATCGCCGACACTCACATCGGCGTGCTTGAAGCGCTGAAAAAATGGGGCATGCCGGTCAGTCGCGAGCTGAAACTGGC
GAATGGCGTCGAGGAGTGCCTGGCCTATTACCGCGATATCGGTGAGCGGCGCCTGACGCTGACTTACGAGATCGACGGTG
TAGTGTTCAAGGTCAACAACCTGGCAGCGCAGCGCGAACTGGGCTTCCGCGCCCGTGAGCCGCGCTGGGCGATTGCTCAT
AAATTCCCCGCCATGGAAGAGCTGACCGAACTGCTGGATGTCGAGTTCCAGGTGGGCCGTACCGGCGCAGTGACGCCGGT
CGCGCGGCTCAAGCCGGTCAAGGTGGCAGGCGTCATGGTCGCCAATGCCACGCTGCATAACATGGACGAAGTGGCGCGGC
TCGGCCTGATGATTGGCGACACGGTGATCATCCGGCGTGCCGGTGACGTGATTCCGCAGGTGGTGCAGGTGGTTGCCGAG
CGCCGCCCCGAGAATGCCAGGGCAGTGCAGGTTCCGCAGACCTGCCCGGTGTGCGGCTCGCATGTCGAGCGCACGCAACT
GATCAAGCGCAGCAAAGGCAAGGAAACCGTCACCGAAGGCGCAGTGTATCGCTGTGTCGGCCGTCTGGCCTGCGGTGCGC
AACTCAAGCAGGCGATCATCCATTACGTGTCGCGTCGCGCCATGGACATCGAAGGGCTGGGTGACAAAACCATTGAGCAA
CTGGTCGATGAGAAACTCATCGGCTCGCCTGCCGATCTGTACAAGCTGAAATACGAACAGATCATCGACCTCGAAGGCTT
TGCTGAAATCTCCAGCAACAAGCTGCTCAAGGCCATCGCCGACAGCCGACAGCCGACCCTGGCGCGCTTCATTTATGCGC
TGGGCATTCCCGATGTGGGTGAAGAGACGGCCAAGGTCCTGGCGCGTTCGCTGGCTTCGCTGGAGCGTGTCAAACAGGCC
TTGCCGGAAGTCTTGACCTACCTGCCGGATGTTGGCCTGGAAGTCGCTTACGAGATTCGCAGCTTCTTCGAGGATGAGCA
CAACCGTAGCGTCATCGATGCGCTGCTCGGTGAATGCGGTCTGCAATTGCAGGATCAGGGCGAGCTGGGCGCCGAGTTCG
CGGCCAGCACCACGCTGGAAGGGCTGATCGACAAACTGCACATTCCGTCGGTAGGACCGGGTGCTGCGCAGAAGCTGGCG
GACCGGTTCGGTACGCTGGAGGCCATCATCAGCGCCGACTGGCTGGACATGCGTCAGGCACTGCCGGAGAAACAGGCCAA
GTCGGTTCGTGACTTCTTCGACGACAGCGCAAACGCAGAGCGCGCCCGCGCCATCGAAGCCCAGCTCAAGGACTTCGGCA
TGCACTGGCGCAGCGAGAAAAAAACCGTCGAAGGCTTGCCGCTGGCCGGGCAGACCTGGGTGCTGACCGGCTCGCTGGAG
CGCATGAGCCGCGATGTCGCCAAGGAAAAACTCGAAAGCCTCGGTGCCAAGGTGTCCGGCTCGGTCTCGGCCAAGACCCA
CACCGTGGTGGCGGGGCCGGGTGCCGGCTCGAAACTGACCAAGGCCAACGAACTGGGCCTCACCGTGCTGGACGAAGATG
CACTGCTCAAACGCCTGATCGAGCTGGGCGTGGCGGTTGATTGA

Upstream 100 bases:

>100_bases
AGCGTGCTGCGGCACGCCCTGTCGGGGCCTGACCTGAACAGAACAGAGCCAACAGATTGAGCAGCCTCGGCTGCTTTTTT
GCTTTTTGAGAGAACACCGT

Downstream 100 bases:

>100_bases
GGAAGGCCGACGCTGCGTATGCCCTGATGATCGTGCGACGCTCCGCGTCGCATGCAGTTCTGGACGCTCTGCGTCCGATC
TTGAGTGTGAGGGTGCGGCG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 787; Mature: 787

Protein sequence:

>787_residues
MKAVETRILELRAELDQHNYRYHVLDEPSIPDVEYDRLFHELKALEAENPHLVTPDSPTQRVGSAALSAFTQVRHEMPML
SLGNAFEENDMLEFDRRVTEGLDLPAGDLFGEGGKVQYSCEPKLDGLAVSLLYRDGALVRGATRGDGTTGEDISVNVRTV
RNIPLKLQGKGWPDVLEVRGEVFMSKAGFERLNASQLEIGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGLGQTSA
EIADTHIGVLEALKKWGMPVSRELKLANGVEECLAYYRDIGERRLTLTYEIDGVVFKVNNLAAQRELGFRAREPRWAIAH
KFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVMVANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVAE
RRPENARAVQVPQTCPVCGSHVERTQLIKRSKGKETVTEGAVYRCVGRLACGAQLKQAIIHYVSRRAMDIEGLGDKTIEQ
LVDEKLIGSPADLYKLKYEQIIDLEGFAEISSNKLLKAIADSRQPTLARFIYALGIPDVGEETAKVLARSLASLERVKQA
LPEVLTYLPDVGLEVAYEIRSFFEDEHNRSVIDALLGECGLQLQDQGELGAEFAASTTLEGLIDKLHIPSVGPGAAQKLA
DRFGTLEAIISADWLDMRQALPEKQAKSVRDFFDDSANAERARAIEAQLKDFGMHWRSEKKTVEGLPLAGQTWVLTGSLE
RMSRDVAKEKLESLGAKVSGSVSAKTHTVVAGPGAGSKLTKANELGLTVLDEDALLKRLIELGVAVD

Sequences:

>Translated_787_residues
MKAVETRILELRAELDQHNYRYHVLDEPSIPDVEYDRLFHELKALEAENPHLVTPDSPTQRVGSAALSAFTQVRHEMPML
SLGNAFEENDMLEFDRRVTEGLDLPAGDLFGEGGKVQYSCEPKLDGLAVSLLYRDGALVRGATRGDGTTGEDISVNVRTV
RNIPLKLQGKGWPDVLEVRGEVFMSKAGFERLNASQLEIGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGLGQTSA
EIADTHIGVLEALKKWGMPVSRELKLANGVEECLAYYRDIGERRLTLTYEIDGVVFKVNNLAAQRELGFRAREPRWAIAH
KFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVMVANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVAE
RRPENARAVQVPQTCPVCGSHVERTQLIKRSKGKETVTEGAVYRCVGRLACGAQLKQAIIHYVSRRAMDIEGLGDKTIEQ
LVDEKLIGSPADLYKLKYEQIIDLEGFAEISSNKLLKAIADSRQPTLARFIYALGIPDVGEETAKVLARSLASLERVKQA
LPEVLTYLPDVGLEVAYEIRSFFEDEHNRSVIDALLGECGLQLQDQGELGAEFAASTTLEGLIDKLHIPSVGPGAAQKLA
DRFGTLEAIISADWLDMRQALPEKQAKSVRDFFDDSANAERARAIEAQLKDFGMHWRSEKKTVEGLPLAGQTWVLTGSLE
RMSRDVAKEKLESLGAKVSGSVSAKTHTVVAGPGAGSKLTKANELGLTVLDEDALLKRLIELGVAVD
>Mature_787_residues
MKAVETRILELRAELDQHNYRYHVLDEPSIPDVEYDRLFHELKALEAENPHLVTPDSPTQRVGSAALSAFTQVRHEMPML
SLGNAFEENDMLEFDRRVTEGLDLPAGDLFGEGGKVQYSCEPKLDGLAVSLLYRDGALVRGATRGDGTTGEDISVNVRTV
RNIPLKLQGKGWPDVLEVRGEVFMSKAGFERLNASQLEIGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGLGQTSA
EIADTHIGVLEALKKWGMPVSRELKLANGVEECLAYYRDIGERRLTLTYEIDGVVFKVNNLAAQRELGFRAREPRWAIAH
KFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVMVANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVAE
RRPENARAVQVPQTCPVCGSHVERTQLIKRSKGKETVTEGAVYRCVGRLACGAQLKQAIIHYVSRRAMDIEGLGDKTIEQ
LVDEKLIGSPADLYKLKYEQIIDLEGFAEISSNKLLKAIADSRQPTLARFIYALGIPDVGEETAKVLARSLASLERVKQA
LPEVLTYLPDVGLEVAYEIRSFFEDEHNRSVIDALLGECGLQLQDQGELGAEFAASTTLEGLIDKLHIPSVGPGAAQKLA
DRFGTLEAIISADWLDMRQALPEKQAKSVRDFFDDSANAERARAIEAQLKDFGMHWRSEKKTVEGLPLAGQTWVLTGSLE
RMSRDVAKEKLESLGAKVSGSVSAKTHTVVAGPGAGSKLTKANELGLTVLDEDALLKRLIELGVAVD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=600, Percent_Identity=56.1666666666667, Blast_Score=671, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 86301; Mature: 86301

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVETRILELRAELDQHNYRYHVLDEPSIPDVEYDRLFHELKALEAENPHLVTPDSPTQ
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHH
RVGSAALSAFTQVRHEMPMLSLGNAFEENDMLEFDRRVTEGLDLPAGDLFGEGGKVQYSC
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCEEEEEC
EPKLDGLAVSLLYRDGALVRGATRGDGTTGEDISVNVRTVRNIPLKLQGKGWPDVLEVRG
CCCCCCEEEEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECCCEEECCCCCHHHHHHHH
EVFMSKAGFERLNASQLEIGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGLGQTSA
HHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHH
EIADTHIGVLEALKKWGMPVSRELKLANGVEECLAYYRDIGERRLTLTYEIDGVVFKVNN
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEECEEEEEECH
LAAQRELGFRAREPRWAIAHKFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVMV
HHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHCCHHCCCCCCCCCHHHHCCCEEEEEEEE
ANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVAERRPENARAVQVPQTCPVCGS
ECHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCC
HVERTQLIKRSKGKETVTEGAVYRCVGRLACGAQLKQAIIHYVSRRAMDIEGLGDKTIEQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LVDEKLIGSPADLYKLKYEQIIDLEGFAEISSNKLLKAIADSRQPTLARFIYALGIPDVG
HHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
EETAKVLARSLASLERVKQALPEVLTYLPDVGLEVAYEIRSFFEDEHNRSVIDALLGECG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
LQLQDQGELGAEFAASTTLEGLIDKLHIPSVGPGAAQKLADRFGTLEAIISADWLDMRQA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPEKQAKSVRDFFDDSANAERARAIEAQLKDFGMHWRSEKKTVEGLPLAGQTWVLTGSLE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHH
RMSRDVAKEKLESLGAKVSGSVSAKTHTVVAGPGAGSKLTKANELGLTVLDEDALLKRLI
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCEEECHHHHHHHHH
ELGVAVD
HHCCCCC
>Mature Secondary Structure
MKAVETRILELRAELDQHNYRYHVLDEPSIPDVEYDRLFHELKALEAENPHLVTPDSPTQ
CCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCHHH
RVGSAALSAFTQVRHEMPMLSLGNAFEENDMLEFDRRVTEGLDLPAGDLFGEGGKVQYSC
HHHHHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHCCCCCEEEEEC
EPKLDGLAVSLLYRDGALVRGATRGDGTTGEDISVNVRTVRNIPLKLQGKGWPDVLEVRG
CCCCCCEEEEEEECCCCEEECCCCCCCCCCCCEEEEEEEEECCCEEECCCCCHHHHHHHH
EVFMSKAGFERLNASQLEIGGKTFANPRNAAAGSLRQLDSKITANRPLEFCCYGLGQTSA
HHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCCCCHH
EIADTHIGVLEALKKWGMPVSRELKLANGVEECLAYYRDIGERRLTLTYEIDGVVFKVNN
HHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEEEEECEEEEEECH
LAAQRELGFRAREPRWAIAHKFPAMEELTELLDVEFQVGRTGAVTPVARLKPVKVAGVMV
HHHHHHCCCCCCCCCCHHHHCCCCHHHHHHHHCCHHCCCCCCCCCHHHHCCCEEEEEEEE
ANATLHNMDEVARLGLMIGDTVIIRRAGDVIPQVVQVVAERRPENARAVQVPQTCPVCGS
ECHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCC
HVERTQLIKRSKGKETVTEGAVYRCVGRLACGAQLKQAIIHYVSRRAMDIEGLGDKTIEQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
LVDEKLIGSPADLYKLKYEQIIDLEGFAEISSNKLLKAIADSRQPTLARFIYALGIPDVG
HHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
EETAKVLARSLASLERVKQALPEVLTYLPDVGLEVAYEIRSFFEDEHNRSVIDALLGECG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCC
LQLQDQGELGAEFAASTTLEGLIDKLHIPSVGPGAAQKLADRFGTLEAIISADWLDMRQA
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPEKQAKSVRDFFDDSANAERARAIEAQLKDFGMHWRSEKKTVEGLPLAGQTWVLTGSLE
CCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCHH
RMSRDVAKEKLESLGAKVSGSVSAKTHTVVAGPGAGSKLTKANELGLTVLDEDALLKRLI
HHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCEEECHHHHHHHHH
ELGVAVD
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA