Click here to switch to the map view.

The map label for this gene is gloB [H]

Identifier: 66045006

GI number: 66045006

Start: 2003028

End: 2003807

Strand: Reverse

Name: gloB [H]

Synonym: Psyr_1761

Alternate gene names: 66045006

Gene position: 2003807-2003028 (Counterclockwise)

Preceding gene: 66045011

Following gene: 66045005

Centisome position: 32.88

GC content: 59.62

Gene sequence:

>780_bases
ATGATACAGATCCACGCCCTTCCTGCTTTCAATGACAACTACATATGGTTGTTACAAGACCTTTCGAGCCAGCAGTGCGC
TGTCGTCGATCCGGGCGACGCAAAGCCGGTGCTGGGCTGGCTGGCGCAGAATCCCGACTACCGGCTGACCGATATCCTGA
TCACGCACCACCACAATGATCATGTGGGCGGTGTTGCGGAACTCAAGCAGTCGACCGCGGCGCGGGTGCTGGGCCCAGCG
GCCGAGACCATACCGGCGCGCGATATCGCGCTGGTCGACAATGACCGGATGACCGTGCTGGGCCTGGAATTTGTGGTGCA
TGCGGTTCCAGGCCACACGCTGGGGCATATCGCGTTTTATCATGAGGACGCCACGACGCCCCTGCTGTTCAGCGGCGACA
CGCTGTTTGCCGCCGGCTGTGGACGGCTGTTCGAGGGCACGCCACAACAGATGCACGATTCTCTCGGCCGCCTGGCCAAA
CTGCCGGACAGCACACTGATCTACTGCGCTCACGAATACACGCTGAGCAACCTGCGCTTTGCCCAGGCCGTCGAGCCCGC
CAATGTCGATATTGCCGAACGCCTGGCCGAAGTGACGCGCTGGCGCAGCGAAAATCGCATCAGTCTTCCTTCGAATATGG
CACTGGAAAAACGCACCAACCCCTTCCTGCGTACCGGGGAAACATCCGTTAAAGAAAAAGCGGACGAACGGAGCGACGCG
CAAAACACCTCGCAGAGTGCGGTCTTTGCAAGCCTGAGGGCATGGAAAGATAAGTTCTGA

Upstream 100 bases:

>100_bases
CGAACGCTTCATCGGTCATGAGTTCTCCTCCAGAGGTGCTCGCATACAGCGGCAACAGACTCTAAGATGCACCATTGTCC
GCCGCTATGCGAACTGCGCC

Downstream 100 bases:

>100_bases
CCCGACCCTAAAACCGCAATAAAATTCTGAAAGGTTGACCGACGGCAGGCTGCTTCCTAGAATCGCCCGACATTTTCGTC
TGGATGTACCCCCCGAACAA

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MIQIHALPAFNDNYIWLLQDLSSQQCAVVDPGDAKPVLGWLAQNPDYRLTDILITHHHNDHVGGVAELKQSTAARVLGPA
AETIPARDIALVDNDRMTVLGLEFVVHAVPGHTLGHIAFYHEDATTPLLFSGDTLFAAGCGRLFEGTPQQMHDSLGRLAK
LPDSTLIYCAHEYTLSNLRFAQAVEPANVDIAERLAEVTRWRSENRISLPSNMALEKRTNPFLRTGETSVKEKADERSDA
QNTSQSAVFASLRAWKDKF

Sequences:

>Translated_259_residues
MIQIHALPAFNDNYIWLLQDLSSQQCAVVDPGDAKPVLGWLAQNPDYRLTDILITHHHNDHVGGVAELKQSTAARVLGPA
AETIPARDIALVDNDRMTVLGLEFVVHAVPGHTLGHIAFYHEDATTPLLFSGDTLFAAGCGRLFEGTPQQMHDSLGRLAK
LPDSTLIYCAHEYTLSNLRFAQAVEPANVDIAERLAEVTRWRSENRISLPSNMALEKRTNPFLRTGETSVKEKADERSDA
QNTSQSAVFASLRAWKDKF
>Mature_259_residues
MIQIHALPAFNDNYIWLLQDLSSQQCAVVDPGDAKPVLGWLAQNPDYRLTDILITHHHNDHVGGVAELKQSTAARVLGPA
AETIPARDIALVDNDRMTVLGLEFVVHAVPGHTLGHIAFYHEDATTPLLFSGDTLFAAGCGRLFEGTPQQMHDSLGRLAK
LPDSTLIYCAHEYTLSNLRFAQAVEPANVDIAERLAEVTRWRSENRISLPSNMALEKRTNPFLRTGETSVKEKADERSDA
QNTSQSAVFASLRAWKDKF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=36.5019011406844, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=36.5019011406844, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI116642887, Length=263, Percent_Identity=34.9809885931559, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI21703352, Length=235, Percent_Identity=37.0212765957447, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI14150041, Length=234, Percent_Identity=34.1880341880342, Blast_Score=141, Evalue=7e-34,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=35.8585858585859, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI41327741, Length=187, Percent_Identity=28.8770053475936, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786406, Length=259, Percent_Identity=44.4015444015444, Blast_Score=204, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17536925, Length=269, Percent_Identity=35.3159851301115, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320478, Length=265, Percent_Identity=28.6792452830189, Blast_Score=109, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6324614, Length=262, Percent_Identity=27.0992366412214, Blast_Score=83, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356335, Length=264, Percent_Identity=36.7424242424242, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24667711, Length=264, Percent_Identity=36.7424242424242, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24667703, Length=264, Percent_Identity=36.7424242424242, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI221330176, Length=174, Percent_Identity=31.6091954022989, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28637; Mature: 28637

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIHALPAFNDNYIWLLQDLSSQQCAVVDPGDAKPVLGWLAQNPDYRLTDILITHHHND
CEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHCCCCCEEEEEEEEECCCC
HVGGVAELKQSTAARVLGPAAETIPARDIALVDNDRMTVLGLEFVVHAVPGHTLGHIAFY
CCCHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCEEEEEHHHHHHHCCCCCEEEEEEE
HEDATTPLLFSGDTLFAAGCGRLFEGTPQQMHDSLGRLAKLPDSTLIYCAHEYTLSNLRF
ECCCCCCEEEECCEEEECCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHCCCH
AQAVEPANVDIAERLAEVTRWRSENRISLPSNMALEKRTNPFLRTGETSVKEKADERSDA
HHCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCHHHCCCCCEECCCHHHHHHHHHHHHH
QNTSQSAVFASLRAWKDKF
CCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIQIHALPAFNDNYIWLLQDLSSQQCAVVDPGDAKPVLGWLAQNPDYRLTDILITHHHND
CEEEEEECCCCCCEEEEEECCCCCCEEEECCCCCCHHHHHHHCCCCCEEEEEEEEECCCC
HVGGVAELKQSTAARVLGPAAETIPARDIALVDNDRMTVLGLEFVVHAVPGHTLGHIAFY
CCCHHHHHHHHHHHHHHCCHHHHCCCCEEEEECCCCEEEEEHHHHHHHCCCCCEEEEEEE
HEDATTPLLFSGDTLFAAGCGRLFEGTPQQMHDSLGRLAKLPDSTLIYCAHEYTLSNLRF
ECCCCCCEEEECCEEEECCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECHHHHCCCH
AQAVEPANVDIAERLAEVTRWRSENRISLPSNMALEKRTNPFLRTGETSVKEKADERSDA
HHCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCHHHCCCCCEECCCHHHHHHHHHHHHH
QNTSQSAVFASLRAWKDKF
CCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA