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The map label for this gene is fabI [H]

Identifier: 66044999

GI number: 66044999

Start: 1992982

End: 1993776

Strand: Reverse

Name: fabI [H]

Synonym: Psyr_1754

Alternate gene names: 66044999

Gene position: 1993776-1992982 (Counterclockwise)

Preceding gene: 66045000

Following gene: 66044997

Centisome position: 32.72

GC content: 62.77

Gene sequence:

>795_bases
ATGGGTTTTCTCGCCGGTAAGCGCGTCCTGATCGTCGGTGTCGCCAGTAAACTGTCCATCGCATCCGGTATTGCCGCCGC
CATGCATCGTGAAGGCGCAGAGCTTGCCTTCACCTACCAGAACGACAAACTCAAGGGTCGTGTCGAAGAGTTCGCCGCAG
GCTGGGGCTCGGGCCCTGAGCTGTGCTTCCCTTGCGACGTGGCCAGCGACGAAGAGATCAACAAGGTCTTCGAAGAGCTG
AGCAAGAAGTGGGACGGCCTGGACGTGATCGTTCACTCGGTGGGCTTCGCGCCTGGCGACCAGCTCGATGGCGACTTCAC
CGAAGCCACCACGCGCGAAGGTTTCCGCATCGCTCATGACATCAGCGCCTACAGCTTCGTGGCCCTGGCCAAGGCCGGTC
GCGAGATGATGAAAGGCCGTAACGGCAGCCTGCTGACCCTGTCGTACCTGGGCGCAGAGCGCACCATGCCCAACTACAAC
GTCATGGGCATGGCCAAGGCCAGCCTGGAAGCCGGCGTTCGTTACCTGGCCGGCAGCCTCGGCCCGGAAGGTACTCGCGT
CAATGCTGTATCGGCCGGCCCGATCCGCACCCTGGCGGCGTCAGGCATCAAGAACTTCCGCAAGATGCTGGCTGCCAACG
AAGCGCAGACCCCTCTGCGCCGCAACGTCACCATCGACGAAGTCGGCAACGCCGGCGCGTTCCTGTGTTCCGACCTGGCG
TCGGGCATCAGCGGTGAAATCATGTACGTCGACGGCGGCTTCAACACCACCGCGATGGGCAGCATGGACGAGTAA

Upstream 100 bases:

>100_bases
GGATATCTTCGCCGCACCGCAACATCCTTATACACAGCAGCTGCTGGAGGCCGCCTTCTTGGCGCCGGTAGCTGTCGATT
AACCTGAAGAGGAACAACAC

Downstream 100 bases:

>100_bases
TACGGTAAACGCCGCTCCTCGCGAGCGGCACTTTCCCGGACTTAAAAAGAAGGCCGCTCAATGAGCGGCCTTTTTGTTGT
CCTGAATTATCCGACTGCTT

Product: Short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MGFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSGPELCFPCDVASDEEINKVFEEL
SKKWDGLDVIVHSVGFAPGDQLDGDFTEATTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
VMGMAKASLEAGVRYLAGSLGPEGTRVNAVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIDEVGNAGAFLCSDLA
SGISGEIMYVDGGFNTTAMGSMDE

Sequences:

>Translated_264_residues
MGFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSGPELCFPCDVASDEEINKVFEEL
SKKWDGLDVIVHSVGFAPGDQLDGDFTEATTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYN
VMGMAKASLEAGVRYLAGSLGPEGTRVNAVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIDEVGNAGAFLCSDLA
SGISGEIMYVDGGFNTTAMGSMDE
>Mature_263_residues
GFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSGPELCFPCDVASDEEINKVFEELS
KKWDGLDVIVHSVGFAPGDQLDGDFTEATTREGFRIAHDISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNV
MGMAKASLEAGVRYLAGSLGPEGTRVNAVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIDEVGNAGAFLCSDLAS
GISGEIMYVDGGFNTTAMGSMDE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Homo sapiens, GI4503301, Length=222, Percent_Identity=27.9279279279279, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI40254992, Length=236, Percent_Identity=28.3898305084746, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787545, Length=263, Percent_Identity=70.3422053231939, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787905, Length=254, Percent_Identity=28.740157480315, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI115534694, Length=257, Percent_Identity=28.0155642023346, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=267, Percent_Identity=26.2172284644195, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24644337, Length=200, Percent_Identity=32, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644339, Length=258, Percent_Identity=27.906976744186, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357041, Length=251, Percent_Identity=28.2868525896414, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28571526, Length=260, Percent_Identity=27.3076923076923, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27936; Mature: 27805

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSGPE
CCCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCC
LCFPCDVASDEEINKVFEELSKKWDGLDVIVHSVGFAPGDQLDGDFTEATTREGFRIAHD
EEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEHH
ISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCC
GPEGTRVNAVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIDEVGNAGAFLCSDLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHCCCCHHHHHHHHH
SGISGEIMYVDGGFNTTAMGSMDE
CCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
GFLAGKRVLIVGVASKLSIASGIAAAMHREGAELAFTYQNDKLKGRVEEFAAGWGSGPE
CCCCCCEEEEEEECHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCCCCCCC
LCFPCDVASDEEINKVFEELSKKWDGLDVIVHSVGFAPGDQLDGDFTEATTREGFRIAHD
EEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCCEEEEHH
ISAYSFVALAKAGREMMKGRNGSLLTLSYLGAERTMPNYNVMGMAKASLEAGVRYLAGSL
HHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCC
GPEGTRVNAVSAGPIRTLAASGIKNFRKMLAANEAQTPLRRNVTIDEVGNAGAFLCSDLA
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHCCCCHHHHHHHHH
SGISGEIMYVDGGFNTTAMGSMDE
CCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10464225; 10984043 [H]