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The map label for this gene is sbcD [H]

Identifier: 66044960

GI number: 66044960

Start: 1943288

End: 1944532

Strand: Reverse

Name: sbcD [H]

Synonym: Psyr_1715

Alternate gene names: 66044960

Gene position: 1944532-1943288 (Counterclockwise)

Preceding gene: 66044961

Following gene: 66044959

Centisome position: 31.91

GC content: 64.66

Gene sequence:

>1245_bases
ATGCGTCTGTTTCACACCTCTGACTGGCACCTGGGTCAGAACCTGCACGGCCAGGAGCGGGATTTCGAACACGCCTGCTT
CCTGACCTGGCTGCTGGCCCGTCTGGCCGACCGTAAACCGGATGTACTGTTGATCGCGGGCGACATCTTCGACACCGTCA
ACCCGCCGGTCAAAGCGCAGGAGCGCCTGTACGACTTCATCGTCAACGCCCACGAACAGCAGCCATTGCTGACCATCGTG
ATGATCGCCGGCAACCATGACTCCGGCTCGCGTATCGAACTTCCCGCCCCGCTGATGCGCCGCTTGCGCACTCACGCGCT
GGGCCGGGTGATGTGGCTGGACGACGGCGTGCTGGATGCCGAACGCCTGCTGTTGCCATTGCCAGACGCCAATGGCGACA
TCAAGGCCTGGTGCCTGGCGCTGCCCTTCCTGCGTCCCGCCGAAGTCACCGGCCCGACCCTGGGCGATGACTACCTGCGT
GGCATCGGCCGGGTGCATGAGCTGCTGATCGCGGCGGCTGACCTCAAGCGCCAGCCCGGTCAGGCATTGATAGCCATCAG
CCACGCCCACATGGCAGGCGGCTCGGTCTCGGAAGACTCCGAGCGCAGCCTGATCATCGGCAACGCCGAAGCCCTGCCGG
CCAGCCTGTTCGGGCCGAGCATCACCTATGTTGCGCTGGGCCACCTGCACAAACCGCAGCGGGTCAACGGCGAAGAGCGC
ATTCGCTACAGCGGCTCGCCGATCCCGCTGTCCTTTTCGGAAATCAGCTACCAGCACCAGATTCTGGAGATCAATTGCGA
CGGCGAGACGCTGACCAGCGTCGAGCCGTTGCTGATCCCTCGCGCCGTCAACCTGCAACGCCTCGGGCCTGCACCATTGG
CCGACCTGCTGGTGCAGCTCAAAGCATTGCCCGACATCGACCTGCTTGCCGACCCGGACCGCCAGCCGTGGCTGGAAGTG
CGCGTGCGTCTGGATGAACCCCAGCCGGACCTGCGCAACCAGATCGAAAACGCCCTACAGGGCAAGGCCGTGCGGCTGGT
GCGCATCGGTGCCGAATACGCCGGTAAAGGCAGCGCGGACGGCAGCGAGGGTGACGCCACGCTGATCGAACTGGACCAGC
TCACGCCTCAGGAACTGTTCAGCCGTGCCTGGCAGGACAACTTCGGCAGCGAGGTCGACGAACAGACGCTGACCGATTTC
GCCACGCTGCTGCGTGAAGTCCAGCAGGAGAGCGAGCAGCCATGA

Upstream 100 bases:

>100_bases
CGAACATATTCAGGACCCGGCAGGTGATGCCGGGTTGCCGCCGCTGTATCCCAAGTAAGGATGCAGCTCAGGCAACACCC
CTATACGACAAGGTAAACCA

Downstream 100 bases:

>100_bases
AAATTCTCGCCATTCGCCTGAAAAACCTGGCCTCGCTGGCCGGACCCTTTGAAATCGATTTCACTGCCGAGCCGCTGGCC
AGCGCCGGACTGTTTGCCAT

Product: exonuclease SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MRLFHTSDWHLGQNLHGQERDFEHACFLTWLLARLADRKPDVLLIAGDIFDTVNPPVKAQERLYDFIVNAHEQQPLLTIV
MIAGNHDSGSRIELPAPLMRRLRTHALGRVMWLDDGVLDAERLLLPLPDANGDIKAWCLALPFLRPAEVTGPTLGDDYLR
GIGRVHELLIAAADLKRQPGQALIAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSITYVALGHLHKPQRVNGEER
IRYSGSPIPLSFSEISYQHQILEINCDGETLTSVEPLLIPRAVNLQRLGPAPLADLLVQLKALPDIDLLADPDRQPWLEV
RVRLDEPQPDLRNQIENALQGKAVRLVRIGAEYAGKGSADGSEGDATLIELDQLTPQELFSRAWQDNFGSEVDEQTLTDF
ATLLREVQQESEQP

Sequences:

>Translated_414_residues
MRLFHTSDWHLGQNLHGQERDFEHACFLTWLLARLADRKPDVLLIAGDIFDTVNPPVKAQERLYDFIVNAHEQQPLLTIV
MIAGNHDSGSRIELPAPLMRRLRTHALGRVMWLDDGVLDAERLLLPLPDANGDIKAWCLALPFLRPAEVTGPTLGDDYLR
GIGRVHELLIAAADLKRQPGQALIAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSITYVALGHLHKPQRVNGEER
IRYSGSPIPLSFSEISYQHQILEINCDGETLTSVEPLLIPRAVNLQRLGPAPLADLLVQLKALPDIDLLADPDRQPWLEV
RVRLDEPQPDLRNQIENALQGKAVRLVRIGAEYAGKGSADGSEGDATLIELDQLTPQELFSRAWQDNFGSEVDEQTLTDF
ATLLREVQQESEQP
>Mature_414_residues
MRLFHTSDWHLGQNLHGQERDFEHACFLTWLLARLADRKPDVLLIAGDIFDTVNPPVKAQERLYDFIVNAHEQQPLLTIV
MIAGNHDSGSRIELPAPLMRRLRTHALGRVMWLDDGVLDAERLLLPLPDANGDIKAWCLALPFLRPAEVTGPTLGDDYLR
GIGRVHELLIAAADLKRQPGQALIAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSITYVALGHLHKPQRVNGEER
IRYSGSPIPLSFSEISYQHQILEINCDGETLTSVEPLLIPRAVNLQRLGPAPLADLLVQLKALPDIDLLADPDRQPWLEV
RVRLDEPQPDLRNQIENALQGKAVRLVRIGAEYAGKGSADGSEGDATLIELDQLTPQELFSRAWQDNFGSEVDEQTLTDF
ATLLREVQQESEQP

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=425, Percent_Identity=31.7647058823529, Blast_Score=174, Evalue=8e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 45899; Mature: 45899

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLFHTSDWHLGQNLHGQERDFEHACFLTWLLARLADRKPDVLLIAGDIFDTVNPPVKAQ
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
ERLYDFIVNAHEQQPLLTIVMIAGNHDSGSRIELPAPLMRRLRTHALGRVMWLDDGVLDA
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHCEEEEECCCCCCC
ERLLLPLPDANGDIKAWCLALPFLRPAEVTGPTLGDDYLRGIGRVHELLIAAADLKRQPG
CCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
QALIAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSITYVALGHLHKPQRVNGEER
CEEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHCCCCCHHHHHHCCCCCCCCCCHHE
IRYSGSPIPLSFSEISYQHQILEINCDGETLTSVEPLLIPRAVNLQRLGPAPLADLLVQL
EEECCCCCCEEHHHHCCEEEEEEEECCCCCCCCCCCEECCCCCCHHHCCCCCHHHHHHHH
KALPDIDLLADPDRQPWLEVRVRLDEPQPDLRNQIENALQGKAVRLVRIGAEYAGKGSAD
HCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECHHHCCCCCCC
GSEGDATLIELDQLTPQELFSRAWQDNFGSEVDEQTLTDFATLLREVQQESEQP
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRLFHTSDWHLGQNLHGQERDFEHACFLTWLLARLADRKPDVLLIAGDIFDTVNPPVKAQ
CCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCHHHH
ERLYDFIVNAHEQQPLLTIVMIAGNHDSGSRIELPAPLMRRLRTHALGRVMWLDDGVLDA
HHHHHHHHCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHHHHCEEEEECCCCCCC
ERLLLPLPDANGDIKAWCLALPFLRPAEVTGPTLGDDYLRGIGRVHELLIAAADLKRQPG
CCEEEECCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
QALIAISHAHMAGGSVSEDSERSLIIGNAEALPASLFGPSITYVALGHLHKPQRVNGEER
CEEEEEEEHHHCCCCCCCCCCCEEEEECCCCCCHHHCCCCCHHHHHHCCCCCCCCCCHHE
IRYSGSPIPLSFSEISYQHQILEINCDGETLTSVEPLLIPRAVNLQRLGPAPLADLLVQL
EEECCCCCCEEHHHHCCEEEEEEEECCCCCCCCCCCEECCCCCCHHHCCCCCHHHHHHHH
KALPDIDLLADPDRQPWLEVRVRLDEPQPDLRNQIENALQGKAVRLVRIGAEYAGKGSAD
HCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECHHHCCCCCCC
GSEGDATLIELDQLTPQELFSRAWQDNFGSEVDEQTLTDFATLLREVQQESEQP
CCCCCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]