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The map label for this gene is yjfC [H]

Identifier: 66044943

GI number: 66044943

Start: 1904792

End: 1905949

Strand: Direct

Name: yjfC [H]

Synonym: Psyr_1698

Alternate gene names: 66044943

Gene position: 1904792-1905949 (Clockwise)

Preceding gene: 66044942

Following gene: 66044944

Centisome position: 31.26

GC content: 59.84

Gene sequence:

>1158_bases
ATGAAAAAGATCAGCATCGAGGAACGTCCGGACTGGCGCACCACCGCCGAGCGTGAAGGGTTCAACTTTCACACCATCGA
TGGCGAGCGTTACTGGGACGAGCGCGGGTATTACCTGTTCAGCGAACAGCAGATTACCCGCGATCTTGAAGCGCCCACCC
AGGAGCTTCACCAGATGTGCATGGATGCCGTGGCGCGAGTGGTGGACAGCGAAGCGCTGCTCCATCAACTGGCGATCCCG
GAAGCGTTCTTCGACCTGATCCAGAGCTCATGGAAGGAGGGACATCCGCATCTGTACGGACGCTTCGATTTTGCCTACGA
CGGCACGGGCCCGGCAAAGATGTACGAAATCAATGCCGATACTCCGACCAGTCTGATGGAAGCCGGGGCGGTGCAACTGC
TCTGGCTGGAAGAGCAGATGGCGCGCGGCGTGCTGCCTGCGCATGCCACCCAGTTCAACTCGATTGCCGAGGATCTGGTT
CGGGCCTTTGCCGAGTTTCCCGGCACGACTCCGTTCTACTTCTCGGCGATGTCCGGTTCCATCGAAGACCGGGGCACCAC
CGATTTTCTGCGGCGTATGGCGGCACACGTCGGCATCGATGCGAGGCATATCGATATCGAAGACATCGGGCTCGGCGCTG
ACGGGCGTTTTGTCGATCTGGACGGGCGCTGGATCGAGCGCTTGTTCAAGCTGCACGCGTGGGAGCATATCTTTCACGAG
GAATTCGGTCAGCACGTGTCAGCGAGCGGCACTCAGTTTGTCGAACCGGCGTGGAAGGCGATTCTCAGCAACAAGGGTAT
TCTGCCGTTGTTGTGGCAGTTCAACGAAGGCCATCCGAACCTGCTGCCCGCGCATGTCGACCAGGACGCGAACAAAGCGG
TCCCCAAAGGCTGGGTACGCAAACCGTACTTTTCCCGTGAGGGCGCCAATATCGAAATGCGCACGCCTGGCGATCAGGTG
ATCTCGGTCGATGGGCCCTACACCGATGCGCCCTACATTCTGCAGGCGTACTCGCCGCTTCCGCGATTCGGCGACAGCTA
CACGCTGATCGGCTCCTGGGTCATCGGCGATCTTGCGTCCGGGATCGGCATTCGTGAAGACGACAGCCTGATCACCAAGG
ACACCAGCCGCTTCCTGCCACACGTCGTCATCGACTGA

Upstream 100 bases:

>100_bases
TCTCGCGCGGCGGCTTCGGCAGCCAGGCCTCGGCACGCAGTGGCTGGGGCGGCAAGAGCAGCAGTGGCAGCAGCAGTTTC
GGAGGCTGACGGTTCGCGGC

Downstream 100 bases:

>100_bases
GCCCTGCCTGTCATCACCCGGTGTTACTGGCTTGAAGCAAGTAACACTCTTGTTGTTACCACACGTTCATATACCCGCCA
TCAAGCAGCGATAATAAGTG

Product: glutathionylspermidine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MKKISIEERPDWRTTAEREGFNFHTIDGERYWDERGYYLFSEQQITRDLEAPTQELHQMCMDAVARVVDSEALLHQLAIP
EAFFDLIQSSWKEGHPHLYGRFDFAYDGTGPAKMYEINADTPTSLMEAGAVQLLWLEEQMARGVLPAHATQFNSIAEDLV
RAFAEFPGTTPFYFSAMSGSIEDRGTTDFLRRMAAHVGIDARHIDIEDIGLGADGRFVDLDGRWIERLFKLHAWEHIFHE
EFGQHVSASGTQFVEPAWKAILSNKGILPLLWQFNEGHPNLLPAHVDQDANKAVPKGWVRKPYFSREGANIEMRTPGDQV
ISVDGPYTDAPYILQAYSPLPRFGDSYTLIGSWVIGDLASGIGIREDDSLITKDTSRFLPHVVID

Sequences:

>Translated_385_residues
MKKISIEERPDWRTTAEREGFNFHTIDGERYWDERGYYLFSEQQITRDLEAPTQELHQMCMDAVARVVDSEALLHQLAIP
EAFFDLIQSSWKEGHPHLYGRFDFAYDGTGPAKMYEINADTPTSLMEAGAVQLLWLEEQMARGVLPAHATQFNSIAEDLV
RAFAEFPGTTPFYFSAMSGSIEDRGTTDFLRRMAAHVGIDARHIDIEDIGLGADGRFVDLDGRWIERLFKLHAWEHIFHE
EFGQHVSASGTQFVEPAWKAILSNKGILPLLWQFNEGHPNLLPAHVDQDANKAVPKGWVRKPYFSREGANIEMRTPGDQV
ISVDGPYTDAPYILQAYSPLPRFGDSYTLIGSWVIGDLASGIGIREDDSLITKDTSRFLPHVVID
>Mature_385_residues
MKKISIEERPDWRTTAEREGFNFHTIDGERYWDERGYYLFSEQQITRDLEAPTQELHQMCMDAVARVVDSEALLHQLAIP
EAFFDLIQSSWKEGHPHLYGRFDFAYDGTGPAKMYEINADTPTSLMEAGAVQLLWLEEQMARGVLPAHATQFNSIAEDLV
RAFAEFPGTTPFYFSAMSGSIEDRGTTDFLRRMAAHVGIDARHIDIEDIGLGADGRFVDLDGRWIERLFKLHAWEHIFHE
EFGQHVSASGTQFVEPAWKAILSNKGILPLLWQFNEGHPNLLPAHVDQDANKAVPKGWVRKPYFSREGANIEMRTPGDQV
ISVDGPYTDAPYILQAYSPLPRFGDSYTLIGSWVIGDLASGIGIREDDSLITKDTSRFLPHVVID

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To the N-terminal of E.coli gsp and to E.coli ygiC [H]

Homologues:

Organism=Escherichia coli, GI1790629, Length=385, Percent_Identity=48.0519480519481, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1789416, Length=388, Percent_Identity=45.6185567010309, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789361, Length=377, Percent_Identity=26.790450928382, Blast_Score=121, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005494
- InterPro:   IPR016185 [H]

Pfam domain/function: PF03738 GSP_synth [H]

EC number: NA

Molecular weight: Translated: 43603; Mature: 43603

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKISIEERPDWRTTAEREGFNFHTIDGERYWDERGYYLFSEQQITRDLEAPTQELHQMC
CCCCCCCCCCCCCCCHHCCCCEEEEECCCHHHCCCCEEEECHHHHHHHHCCHHHHHHHHH
MDAVARVVDSEALLHQLAIPEAFFDLIQSSWKEGHPHLYGRFDFAYDGTGPAKMYEINAD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCC
TPTSLMEAGAVQLLWLEEQMARGVLPAHATQFNSIAEDLVRAFAEFPGTTPFYFSAMSGS
CCHHHHHCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
IEDRGTTDFLRRMAAHVGIDARHIDIEDIGLGADGRFVDLDGRWIERLFKLHAWEHIFHE
CCCCCHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
EFGQHVSASGTQFVEPAWKAILSNKGILPLLWQFNEGHPNLLPAHVDQDANKAVPKGWVR
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
KPYFSREGANIEMRTPGDQVISVDGPYTDAPYILQAYSPLPRFGDSYTLIGSWVIGDLAS
CCCCCCCCCEEEEECCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHH
GIGIREDDSLITKDTSRFLPHVVID
CCCCCCCCCHHHCCHHHHCCEEECC
>Mature Secondary Structure
MKKISIEERPDWRTTAEREGFNFHTIDGERYWDERGYYLFSEQQITRDLEAPTQELHQMC
CCCCCCCCCCCCCCCHHCCCCEEEEECCCHHHCCCCEEEECHHHHHHHHCCHHHHHHHHH
MDAVARVVDSEALLHQLAIPEAFFDLIQSSWKEGHPHLYGRFDFAYDGTGPAKMYEINAD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEECCC
TPTSLMEAGAVQLLWLEEQMARGVLPAHATQFNSIAEDLVRAFAEFPGTTPFYFSAMSGS
CCHHHHHCCCEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
IEDRGTTDFLRRMAAHVGIDARHIDIEDIGLGADGRFVDLDGRWIERLFKLHAWEHIFHE
CCCCCHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
EFGQHVSASGTQFVEPAWKAILSNKGILPLLWQFNEGHPNLLPAHVDQDANKAVPKGWVR
HHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
KPYFSREGANIEMRTPGDQVISVDGPYTDAPYILQAYSPLPRFGDSYTLIGSWVIGDLAS
CCCCCCCCCEEEEECCCCCEEECCCCCCCCCHHHHHCCCCCCCCCCEEEEHHHHHHHHHH
GIGIREDDSLITKDTSRFLPHVVID
CCCCCCCCCHHHCCHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 7961409 [H]