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The map label for this gene is cysK [H]

Identifier: 66044828

GI number: 66044828

Start: 1777829

End: 1778803

Strand: Direct

Name: cysK [H]

Synonym: Psyr_1583

Alternate gene names: 66044828

Gene position: 1777829-1778803 (Clockwise)

Preceding gene: 66044826

Following gene: 66044829

Centisome position: 29.17

GC content: 60.62

Gene sequence:

>975_bases
ATGAGCCGTATCTACGCTGATAACGCCCATTCCATCGGTAATACGCCGTTGGTGCAGATCAACCGTATCGCGCCGCGCGG
TGTCACCATTCTGGCCAAGATCGAAGGGCGTAATCCGGGCTATTCCGTCAAGTGCCGTATCGGCGCCAACATGATCTGGG
ACGCCGAAAGCAGCGGCAGGCTCAAACCGGGCATGACCATCGTCGAGCCGACCTCTGGCAACACCGGCATCGGTCTGGCC
TTCGTTGCCGCTGCCCGTGGCTACAAGCTGATGCTCACCATGCCCGCTTCCATGAGCATAGAACGTCGCAAGGTCCTCAA
GGCACTGGGCGCAGAACTGGTGCTGACCGAAGCGGCCAAGGGCATGAAAGGCGCGATTGCCAAGGCCGAAGAACTGCTGG
CAGGCAACCCCGGGAAATATTTCATGCCGGCGCAATTCGAAAACCCGGCCAACCCGGCCATCCATGAAAAAACCACTGGC
CCGGAAATCTGGGCAGACACCGACGGTGCCATCGATGTCCTGGTGGCAGGCGTAGGAACTGGCGGGACTATCACCGGTAT
CTCCCGGTATATCAAGCACACCGAAGGCAAACCGATCCTGTCGGTCGCGGTCGAGCCGGACGGTTCGCCGATCATCACCC
AGGCGATGGCCGGTGAAACGATCACGCCCAGCCCGCACAAGATTCAGGGCATCGGCGCCGGCTTCATCCCGAAAAACCTC
GACCTGTCGATTGTCGACCGGGTGGAGCGTGTCACCGATGAAGAGTCCAAGGCCATGGCGCGGCGTCTGATGCAGGAGGA
GGGCATTCTCTGCGGTATTTCGTGCGGCGCGGCAATGGCGGCGGCCGTCCGTCTGGCTGAAAAGCCGGAGATGCAGGGCA
AGACGATCGTGGTGGTCCTGCCGGACTCCGGCGAACGTTACCTGTCGAGCATGCTGTTCAGCGATCTGTTTACCGATCAG
GAGCTTTCCCAGTAA

Upstream 100 bases:

>100_bases
GATTGACTGCTCGCGCCGTCATCCGTTCGGTCAGACTGGACGCCCCACAGTTGGCGGCATAGGCTTTGGATCTTTAACCG
ATGTGCACAAGGACACCCTC

Downstream 100 bases:

>100_bases
CACATCTTCGCAAGCGCGCACTTCATGCGGGCAACGCAAGGGTTTATGATGGCCAGCTTACGACTCGCCGGACGATCAGC
GCTGTCCGCCCGGCGCTCCA

Product: cysteine synthase K/M:cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSRIYADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGRLKPGMTIVEPTSGNTGIGLA
FVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEAAKGMKGAIAKAEELLAGNPGKYFMPAQFENPANPAIHEKTTG
PEIWADTDGAIDVLVAGVGTGGTITGISRYIKHTEGKPILSVAVEPDGSPIITQAMAGETITPSPHKIQGIGAGFIPKNL
DLSIVDRVERVTDEESKAMARRLMQEEGILCGISCGAAMAAAVRLAEKPEMQGKTIVVVLPDSGERYLSSMLFSDLFTDQ
ELSQ

Sequences:

>Translated_324_residues
MSRIYADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGRLKPGMTIVEPTSGNTGIGLA
FVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEAAKGMKGAIAKAEELLAGNPGKYFMPAQFENPANPAIHEKTTG
PEIWADTDGAIDVLVAGVGTGGTITGISRYIKHTEGKPILSVAVEPDGSPIITQAMAGETITPSPHKIQGIGAGFIPKNL
DLSIVDRVERVTDEESKAMARRLMQEEGILCGISCGAAMAAAVRLAEKPEMQGKTIVVVLPDSGERYLSSMLFSDLFTDQ
ELSQ
>Mature_323_residues
SRIYADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGRLKPGMTIVEPTSGNTGIGLAF
VAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEAAKGMKGAIAKAEELLAGNPGKYFMPAQFENPANPAIHEKTTGP
EIWADTDGAIDVLVAGVGTGGTITGISRYIKHTEGKPILSVAVEPDGSPIITQAMAGETITPSPHKIQGIGAGFIPKNLD
LSIVDRVERVTDEESKAMARRLMQEEGILCGISCGAAMAAAVRLAEKPEMQGKTIVVVLPDSGERYLSSMLFSDLFTDQE
LSQ

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=326, Percent_Identity=42.9447852760736, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI295821200, Length=326, Percent_Identity=42.9447852760736, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI4557415, Length=326, Percent_Identity=42.9447852760736, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1788754, Length=325, Percent_Identity=69.5384615384615, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI2367138, Length=314, Percent_Identity=40.7643312101911, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI17535051, Length=308, Percent_Identity=50.974025974026, Blast_Score=283, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=309, Percent_Identity=55.3398058252427, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI17562970, Length=330, Percent_Identity=45.7575757575758, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32566674, Length=314, Percent_Identity=46.4968152866242, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17561720, Length=324, Percent_Identity=46.6049382716049, Blast_Score=240, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI32566672, Length=232, Percent_Identity=45.6896551724138, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=41.5625, Blast_Score=167, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI25147552, Length=324, Percent_Identity=37.6543209876543, Blast_Score=156, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=43.5114503816794, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321594, Length=326, Percent_Identity=38.9570552147239, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=336, Percent_Identity=34.8214285714286, Blast_Score=143, Evalue=4e-35,
Organism=Drosophila melanogaster, GI24643623, Length=320, Percent_Identity=40.9375, Blast_Score=182, Evalue=3e-46,
Organism=Drosophila melanogaster, GI20129101, Length=320, Percent_Identity=40.9375, Blast_Score=182, Evalue=3e-46,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34330; Mature: 34199

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIYADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGR
CCCEECCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCEEEEECCCCCC
LKPGMTIVEPTSGNTGIGLAFVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEAAK
CCCCCEEEECCCCCCCCHHHEEHHCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHC
GMKGAIAKAEELLAGNPGKYFMPAQFENPANPAIHEKTTGPEIWADTDGAIDVLVAGVGT
CHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCC
GGTITGISRYIKHTEGKPILSVAVEPDGSPIITQAMAGETITPSPHKIQGIGAGFIPKNL
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHHCCCCCCCCCCEEEECCCCCCCCCC
DLSIVDRVERVTDEESKAMARRLMQEEGILCGISCGAAMAAAVRLAEKPEMQGKTIVVVL
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCEEEEEE
PDSGERYLSSMLFSDLFTDQELSQ
CCCCHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
SRIYADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESSGR
CCEECCCCCCCCCCCEEEEEEECCCCEEEEEEEECCCCCEEEEEEECCEEEEECCCCCC
LKPGMTIVEPTSGNTGIGLAFVAAARGYKLMLTMPASMSIERRKVLKALGAELVLTEAAK
CCCCCEEEECCCCCCCCHHHEEHHCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEHHHHC
GMKGAIAKAEELLAGNPGKYFMPAQFENPANPAIHEKTTGPEIWADTDGAIDVLVAGVGT
CHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCC
GGTITGISRYIKHTEGKPILSVAVEPDGSPIITQAMAGETITPSPHKIQGIGAGFIPKNL
CCHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEHHHCCCCCCCCCCEEEECCCCCCCCCC
DLSIVDRVERVTDEESKAMARRLMQEEGILCGISCGAAMAAAVRLAEKPEMQGKTIVVVL
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHCCCCCCCCEEEEEE
PDSGERYLSSMLFSDLFTDQELSQ
CCCCHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]