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The map label for this gene is eno [H]

Identifier: 66044729

GI number: 66044729

Start: 1670787

End: 1672070

Strand: Reverse

Name: eno [H]

Synonym: Psyr_1482

Alternate gene names: 66044729

Gene position: 1672070-1670787 (Counterclockwise)

Preceding gene: 66044730

Following gene: 66044728

Centisome position: 27.44

GC content: 53.58

Gene sequence:

>1284_bases
ATGAAAACGCAAATCCAGGCCATTCATGCACGTGAAATTCTCGATTCACGCGGTAACCCTACCGTTGAAGTCGACGTCAC
CTTGGAGTGCGGCGCCATGGGCCGGGCTTCAGTACCGTCCGGTGCCTCTACAGGCGCTCACGAAGCAGTTGAGTTGCGTG
ACAAAGATACACAGCGGTACTCCGGCAAGGGAGTGTTAAAGGCCGTTAGCAATGTGAATACCGAAATACTTGAGTCTCTG
AGGGGCATGAATGCGATTGACCAGGAACAAATTGATCATTTGATGATTAAGCTGGATGGAACCTCTGATAAATCCAGGCT
TGGAGGAAACGCGATCTTGGGCGTGAGCCTCGCCGTTGCCAGAGCGGCGGCTTCTGCGCTGAACTTGCCCTTGTTCCAAT
ATCTGGGCGGCGAGCAAGCAGCAAGAATGCCCGTCCCGATGTTCAATATTCTCAATGGCGGTGTTCACGCCAACTGGCAA
GGCCCTGACTTTCAGGAATTCATGATTGCACCTACAGGGGCTGGCAGTTTCAAGGAAGCCTTGCGATGGGGCTCCGAGGT
GTACCACGAGCTGAAAGCGGTGCTTAAGGACGCCGGCTATTCAACAGCGGTCGGAGATGAGGGCGGCTTTGCGCCGGCAC
TCAAGAAAAACTCAGACGCCATTGAGCTGATCATCAAGGCAATCGAGAGGGCTGGTTACACCCCTGGATCTCAGATCGAG
ATCGCTATCGATCCAGCATCCAGCGGCTTCTATGAAAACGGCCTTTACCATCTGCGCTCTGAGGGTCGTAAGGTCGATGC
TCAAGAGCTGATTAATCTCTATTCCTCCTGGGTCGACAAGTATCCGATTGCGGTACTGGAAGATGGACTCGCGGAAGACG
ATTGGTCAGGCTGGAAGCTATTGAATGCAGCTCTGGGTGACCGGATCGAGTTGGTTGGAGATGACCTATTTGTCACCAAT
GTCGAACGGATTCAGCGAGGCATCACAGAAAACGTCGCCAATGCCGTATTGATCAAACCTAATCAAATCGGAACGCTGAC
TGAGACCAAAGCCGCCATCGAAATGGCCTATGGGGCAAATTGGGGGGCGATGGTTTCTCATCGTAGCGGGGAGACGGTGG
ACAGCTCCATCGCTGATCTGACTGTCGCGATGGGGACTGGCCATCTCAAGACAGGAGCTCCATGCCGGGGGGAGCGGGTC
GAGAAATACAATCAGTTTCTGCGAATAGAAGAGGATCTTGGCAGCCGTGCGTTTTATGCAGGACATGATGCATTCGTGCG
CTGA

Upstream 100 bases:

>100_bases
CTGTTCAGGATACTCATGGAGAGCTGACGTCCGACATAGAGGCCTCTCAACGGATTCAAATGGTTAATGACGCTCCCGAA
TCGTTACTTGGAGAAGATCA

Downstream 100 bases:

>100_bases
CGGCCTATCGCCGGTAAACCTGTTGGACTGGTTACCGTAAAAAAACGAACCACGTATAGCTGTTGGCAAAAACGTCAGTG
GCATTGGCCTTTCCTAGACG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 2; 2-phosphoglycerate dehydratase 2 [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKTQIQAIHAREILDSRGNPTVEVDVTLECGAMGRASVPSGASTGAHEAVELRDKDTQRYSGKGVLKAVSNVNTEILESL
RGMNAIDQEQIDHLMIKLDGTSDKSRLGGNAILGVSLAVARAAASALNLPLFQYLGGEQAARMPVPMFNILNGGVHANWQ
GPDFQEFMIAPTGAGSFKEALRWGSEVYHELKAVLKDAGYSTAVGDEGGFAPALKKNSDAIELIIKAIERAGYTPGSQIE
IAIDPASSGFYENGLYHLRSEGRKVDAQELINLYSSWVDKYPIAVLEDGLAEDDWSGWKLLNAALGDRIELVGDDLFVTN
VERIQRGITENVANAVLIKPNQIGTLTETKAAIEMAYGANWGAMVSHRSGETVDSSIADLTVAMGTGHLKTGAPCRGERV
EKYNQFLRIEEDLGSRAFYAGHDAFVR

Sequences:

>Translated_427_residues
MKTQIQAIHAREILDSRGNPTVEVDVTLECGAMGRASVPSGASTGAHEAVELRDKDTQRYSGKGVLKAVSNVNTEILESL
RGMNAIDQEQIDHLMIKLDGTSDKSRLGGNAILGVSLAVARAAASALNLPLFQYLGGEQAARMPVPMFNILNGGVHANWQ
GPDFQEFMIAPTGAGSFKEALRWGSEVYHELKAVLKDAGYSTAVGDEGGFAPALKKNSDAIELIIKAIERAGYTPGSQIE
IAIDPASSGFYENGLYHLRSEGRKVDAQELINLYSSWVDKYPIAVLEDGLAEDDWSGWKLLNAALGDRIELVGDDLFVTN
VERIQRGITENVANAVLIKPNQIGTLTETKAAIEMAYGANWGAMVSHRSGETVDSSIADLTVAMGTGHLKTGAPCRGERV
EKYNQFLRIEEDLGSRAFYAGHDAFVR
>Mature_427_residues
MKTQIQAIHAREILDSRGNPTVEVDVTLECGAMGRASVPSGASTGAHEAVELRDKDTQRYSGKGVLKAVSNVNTEILESL
RGMNAIDQEQIDHLMIKLDGTSDKSRLGGNAILGVSLAVARAAASALNLPLFQYLGGEQAARMPVPMFNILNGGVHANWQ
GPDFQEFMIAPTGAGSFKEALRWGSEVYHELKAVLKDAGYSTAVGDEGGFAPALKKNSDAIELIIKAIERAGYTPGSQIE
IAIDPASSGFYENGLYHLRSEGRKVDAQELINLYSSWVDKYPIAVLEDGLAEDDWSGWKLLNAALGDRIELVGDDLFVTN
VERIQRGITENVANAVLIKPNQIGTLTETKAAIEMAYGANWGAMVSHRSGETVDSSIADLTVAMGTGHLKTGAPCRGERV
EKYNQFLRIEEDLGSRAFYAGHDAFVR

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=431, Percent_Identity=51.9721577726218, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=50.3480278422274, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=50.8120649651972, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=50.8120649651972, Blast_Score=410, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=46.1538461538462, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI169201331, Length=351, Percent_Identity=26.2108262108262, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI169201757, Length=351, Percent_Identity=26.2108262108262, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI239744207, Length=351, Percent_Identity=26.2108262108262, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=52.9411764705882, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71995829, Length=436, Percent_Identity=51.3761467889908, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17536383, Length=433, Percent_Identity=51.7321016166282, Blast_Score=415, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=192, Percent_Identity=47.3958333333333, Blast_Score=186, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=51.0392609699769, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.8083140877598, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.8083140877598, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=49.0783410138249, Blast_Score=378, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=48.6175115207373, Blast_Score=366, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=52.2041763341067, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=52.2041763341067, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=52.2041763341067, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=52.2041763341067, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=52.2041763341067, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=52.2041763341067, Blast_Score=402, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46037; Mature: 46037

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQIQAIHAREILDSRGNPTVEVDVTLECGAMGRASVPSGASTGAHEAVELRDKDTQRY
CCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHH
SGKGVLKAVSNVNTEILESLRGMNAIDQEQIDHLMIKLDGTSDKSRLGGNAILGVSLAVA
CCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHCEEEEEEECCCCHHHCCCCEEHHHHHHHH
RAAASALNLPLFQYLGGEQAARMPVPMFNILNGGVHANWQGPDFQEFMIAPTGAGSFKEA
HHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHCCCEECCCCCCCHHHEEEECCCCCHHHHH
LRWGSEVYHELKAVLKDAGYSTAVGDEGGFAPALKKNSDAIELIIKAIERAGYTPGSQIE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
IAIDPASSGFYENGLYHLRSEGRKVDAQELINLYSSWVDKYPIAVLEDGLAEDDWSGWKL
EEECCCCCCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
LNAALGDRIELVGDDLFVTNVERIQRGITENVANAVLIKPNQIGTLTETKAAIEMAYGAN
HHHHHCCCEEEECCCCEEECHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCC
WGAMVSHRSGETVDSSIADLTVAMGTGHLKTGAPCRGERVEKYNQFLRIEEDLGSRAFYA
CCCEEECCCCCCHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHEEEHHHCCCCEEEC
GHDAFVR
CCHHHCC
>Mature Secondary Structure
MKTQIQAIHAREILDSRGNPTVEVDVTLECGAMGRASVPSGASTGAHEAVELRDKDTQRY
CCCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHCCCCCHHH
SGKGVLKAVSNVNTEILESLRGMNAIDQEQIDHLMIKLDGTSDKSRLGGNAILGVSLAVA
CCCHHHHHHHCCCHHHHHHHHCCCCCCHHHHCEEEEEEECCCCHHHCCCCEEHHHHHHHH
RAAASALNLPLFQYLGGEQAARMPVPMFNILNGGVHANWQGPDFQEFMIAPTGAGSFKEA
HHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHCCCEECCCCCCCHHHEEEECCCCCHHHHH
LRWGSEVYHELKAVLKDAGYSTAVGDEGGFAPALKKNSDAIELIIKAIERAGYTPGSQIE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEE
IAIDPASSGFYENGLYHLRSEGRKVDAQELINLYSSWVDKYPIAVLEDGLAEDDWSGWKL
EEECCCCCCCHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHH
LNAALGDRIELVGDDLFVTNVERIQRGITENVANAVLIKPNQIGTLTETKAAIEMAYGAN
HHHHHCCCEEEECCCCEEECHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHHHHCCCC
WGAMVSHRSGETVDSSIADLTVAMGTGHLKTGAPCRGERVEKYNQFLRIEEDLGSRAFYA
CCCEEECCCCCCHHHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHEEEHHHCCCCEEEC
GHDAFVR
CCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]