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The map label for this gene is pbpE [H]

Identifier: 66044686

GI number: 66044686

Start: 1628265

End: 1629257

Strand: Direct

Name: pbpE [H]

Synonym: Psyr_1439

Alternate gene names: 66044686

Gene position: 1628265-1629257 (Clockwise)

Preceding gene: 66044685

Following gene: 66044706

Centisome position: 26.72

GC content: 46.02

Gene sequence:

>993_bases
ATGACTATTATTGGATCAAAACACGAAAACCCTGGATTTGTCTCTTTGCTCTGTCTCGACCAAGGTAGGTGCTATGAGCG
AAGTATTGGCGTGGAATGTATCGCAAACCAACGGCCATTGAATCGCGATACCATCTTCGATCTAGCTTCAGTCTCCAAAC
AATTCACCGCGCTTTGCTTGCTGCTGCTGGAGCAAGAGGGGCTTTTAACTATGAGCGACCCTATTGGCCGCTTCGTACCG
GAAGTAAGCTCTTACTCTGCCAAAGTTACCTTGCAGGATCTGATCTATCACATTAGCGGAATGCCTGACTTCATAGAAAT
TGCGCTGGCGAATGGCATTAAATTCGAAGATGAACTTAGTTCCACCGACATACTTGCACTGCTGAAGACTCAGACCAAAC
TTAACTTTGCGCCAGGCACACGATTTGAGTACAGCAATACAGGTTATTTCCTGCTGGGGATAGTCATAGAGCGCGTAAGT
GGCATTCCCTATGCAGAGTATGCCAAAACCTGTATTTTTACACCCCTAGGTATGCGACACACCTTCATTCTAGACGGTAT
GAGCCAAGATATGCGCACTGCGACCGGGTATGCAAAATCTCACAGCGGGACTTTTGCTATCTCACGAAATCCTTGGAATT
CTTTGGGTGCGAGCTTAGTACATTCATCCGCCGCAGATCTTATGAAGTGGGGCAAGAATTTCCTCACGGCTACAGTAGGG
GGGTATGTTGTTATACAGAAGATGTTAACCCCGCTCGCTAAAAAAAATCAAAGGGGCGAAGCTATAATTGAATATTCGCC
CTATTGCTTCGGGATATCCATGGATAATGACGGTGCAGGCATTAAATTCTGCCATGAGGGGGGTACGGCAGGCTTTAGTA
CATATTTCGCGCGCTCATTAGATAAAGGCTATACTTTGGCCGTCTTATCAAATATAGAAGACTATGATGTTGAGGAGCTA
GCCTGTAGTTTGTGTGTAGGGCATGGTCTCTAA

Upstream 100 bases:

>100_bases
GAAATTGAGCAGTTGCTTCCGCATAAGTGGAAACCGGTTCAGTTATTTAATCCGTAATTTTGGACGCTTAATTTTCTATA
AATTAATATTGGGTTCAACC

Downstream 100 bases:

>100_bases
CGACTTCCTGTATGAATCATGCAATCATATCTCTTATTGAAAACCTTCATCACTGCGCGGATTATATACAAATAAATTGT
GCCACAAATCTTGCAGCGCT

Product: Beta-lactamase

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTIIGSKHENPGFVSLLCLDQGRCYERSIGVECIANQRPLNRDTIFDLASVSKQFTALCLLLLEQEGLLTMSDPIGRFVP
EVSSYSAKVTLQDLIYHISGMPDFIEIALANGIKFEDELSSTDILALLKTQTKLNFAPGTRFEYSNTGYFLLGIVIERVS
GIPYAEYAKTCIFTPLGMRHTFILDGMSQDMRTATGYAKSHSGTFAISRNPWNSLGASLVHSSAADLMKWGKNFLTATVG
GYVVIQKMLTPLAKKNQRGEAIIEYSPYCFGISMDNDGAGIKFCHEGGTAGFSTYFARSLDKGYTLAVLSNIEDYDVEEL
ACSLCVGHGL

Sequences:

>Translated_330_residues
MTIIGSKHENPGFVSLLCLDQGRCYERSIGVECIANQRPLNRDTIFDLASVSKQFTALCLLLLEQEGLLTMSDPIGRFVP
EVSSYSAKVTLQDLIYHISGMPDFIEIALANGIKFEDELSSTDILALLKTQTKLNFAPGTRFEYSNTGYFLLGIVIERVS
GIPYAEYAKTCIFTPLGMRHTFILDGMSQDMRTATGYAKSHSGTFAISRNPWNSLGASLVHSSAADLMKWGKNFLTATVG
GYVVIQKMLTPLAKKNQRGEAIIEYSPYCFGISMDNDGAGIKFCHEGGTAGFSTYFARSLDKGYTLAVLSNIEDYDVEEL
ACSLCVGHGL
>Mature_329_residues
TIIGSKHENPGFVSLLCLDQGRCYERSIGVECIANQRPLNRDTIFDLASVSKQFTALCLLLLEQEGLLTMSDPIGRFVPE
VSSYSAKVTLQDLIYHISGMPDFIEIALANGIKFEDELSSTDILALLKTQTKLNFAPGTRFEYSNTGYFLLGIVIERVSG
IPYAEYAKTCIFTPLGMRHTFILDGMSQDMRTATGYAKSHSGTFAISRNPWNSLGASLVHSSAADLMKWGKNFLTATVGG
YVVIQKMLTPLAKKNQRGEAIIEYSPYCFGISMDNDGAGIKFCHEGGTAGFSTYFARSLDKGYTLAVLSNIEDYDVEELA
CSLCVGHGL

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=234, Percent_Identity=26.0683760683761, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082101, Length=189, Percent_Identity=28.042328042328, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1786574, Length=230, Percent_Identity=26.5217391304348, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17550934, Length=178, Percent_Identity=28.0898876404494, Blast_Score=74, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 36156; Mature: 36025

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00146 BETA_LACTAMASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIGSKHENPGFVSLLCLDQGRCYERSIGVECIANQRPLNRDTIFDLASVSKQFTALCL
CCEECCCCCCCCEEEEEEECCCCCCHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LLLEQEGLLTMSDPIGRFVPEVSSYSAKVTLQDLIYHISGMPDFIEIALANGIKFEDELS
HHHCCCCCEEECCHHHHHHCCCCCCEEEEEHHHHHHHHCCCCHHHHHHHHCCCCCHHHCC
STDILALLKTQTKLNFAPGTRFEYSNTGYFLLGIVIERVSGIPYAEYAKTCIFTPLGMRH
CCHHHHEEHHHCEEECCCCCCEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCE
TFILDGMSQDMRTATGYAKSHSGTFAISRNPWNSLGASLVHSSAADLMKWGKNFLTATVG
EEEECCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GYVVIQKMLTPLAKKNQRGEAIIEYSPYCFGISMDNDGAGIKFCHEGGTAGFSTYFARSL
HHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHH
DKGYTLAVLSNIEDYDVEELACSLCVGHGL
CCCEEEEEECCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIIGSKHENPGFVSLLCLDQGRCYERSIGVECIANQRPLNRDTIFDLASVSKQFTALCL
CEECCCCCCCCEEEEEEECCCCCCHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
LLLEQEGLLTMSDPIGRFVPEVSSYSAKVTLQDLIYHISGMPDFIEIALANGIKFEDELS
HHHCCCCCEEECCHHHHHHCCCCCCEEEEEHHHHHHHHCCCCHHHHHHHHCCCCCHHHCC
STDILALLKTQTKLNFAPGTRFEYSNTGYFLLGIVIERVSGIPYAEYAKTCIFTPLGMRH
CCHHHHEEHHHCEEECCCCCCEEECCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCCCCCE
TFILDGMSQDMRTATGYAKSHSGTFAISRNPWNSLGASLVHSSAADLMKWGKNFLTATVG
EEEECCCCHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
GYVVIQKMLTPLAKKNQRGEAIIEYSPYCFGISMDNDGAGIKFCHEGGTAGFSTYFARSL
HHHHHHHHHHHHHHCCCCCCEEEEECCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHH
DKGYTLAVLSNIEDYDVEELACSLCVGHGL
CCCEEEEEECCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]