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The map label for this gene is yafC [H]

Identifier: 66044683

GI number: 66044683

Start: 1623981

End: 1624916

Strand: Reverse

Name: yafC [H]

Synonym: Psyr_1435

Alternate gene names: 66044683

Gene position: 1624916-1623981 (Counterclockwise)

Preceding gene: 66044687

Following gene: 66044682

Centisome position: 26.67

GC content: 54.81

Gene sequence:

>936_bases
ATGATGCGACTGGGCGACTTGAAACTGTTTGTGCGCACTGTAAAGCTTGGAAGTTTCTCCAACGCGGCACGCGAGGCTGA
CCTTTTGCCGGGACAAGTCAGCGCTGCCATAAAGCGTCTGGAGCACGAATTGGGTATCCGCTTGTTCGCCCGCTCTACCC
GTAGTTTGCGTCTGACTGGAGAGGGGGAAACGTACCTCCCCAGCGCGATTACCGCATTGGACGCTCTGGCTCAGGGGCAG
GAGAGATTGCACGGTGAAGGCAACCAATTGCAGGGGATATTGCAGGTCGCCGCGCCATCTGATCTAGGCAGAAACGTGTT
GTTATCCTGCTTCAGCCAATTTCGCAAAGAACACCCACAGCTTACTTTGCGCCTGCTGATTTCTGATCGGCTGGCCAATG
TTTTCCGTGACCCAGTCGACATCGCCATTCGTTATGGCAGCGCGGAAGATGCCAGTTACGTAGAACGGCCTTTGGCCGCC
TGGAATCGACGGGTGCTGGTTGCATCACCGGCTTACCTTTCCCGTCATGGTAGGCCGGCCATGCTGGAAGATTTGAGTCA
TCATTGCTGCCTTCTGTACATGCTCAATGAGCACGTCTATGACAATTGGAAGATTGCGCAGCAGAAGATTCCTGTCAAAG
GCCTATTGGTGAGTGACGATGCAGATGTCGTTAGACTTTGGGCGATTGCAGGCGAAGGGATTGCGTACAAGTCCTGGCTT
GATGTCCGCGAGGATGTCCTTAGCGGTCGTCTGGAAATTTTGCTTGGACAGCATCCTGGGGAATTGAGTCCGTTAAATCT
GGTCTGTCCGCACCGCAAGCAATACTCGCCGGCAGTACGGCAGTTGCACCAGATGCTTAGCCGCCATTTAGCGCCCTTCG
CTGCGGATATGGCCGCAGCGTTGGCGAATGCCGAAATCCCTGACTCGACTGCGTAA

Upstream 100 bases:

>100_bases
TGAGCCTAGCCTCGTCTCGTGGCCAGGCGGGCATACTGACCGGTATATTGCGACGTAAAAAGCCGGTAAATTCACCAGCA
CTTTCACTCGAATAGAGAAA

Downstream 100 bases:

>100_bases
TCAGTTGCTTGTAAGGGTGATGCCTGAGGTTTGATTCTTGAAAGGCGAGTTCTGAATTCGTTGATTGCTGACTACTGGGG
TATGGCGTTGAGGGGTAGTT

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MMRLGDLKLFVRTVKLGSFSNAAREADLLPGQVSAAIKRLEHELGIRLFARSTRSLRLTGEGETYLPSAITALDALAQGQ
ERLHGEGNQLQGILQVAAPSDLGRNVLLSCFSQFRKEHPQLTLRLLISDRLANVFRDPVDIAIRYGSAEDASYVERPLAA
WNRRVLVASPAYLSRHGRPAMLEDLSHHCCLLYMLNEHVYDNWKIAQQKIPVKGLLVSDDADVVRLWAIAGEGIAYKSWL
DVREDVLSGRLEILLGQHPGELSPLNLVCPHRKQYSPAVRQLHQMLSRHLAPFAADMAAALANAEIPDSTA

Sequences:

>Translated_311_residues
MMRLGDLKLFVRTVKLGSFSNAAREADLLPGQVSAAIKRLEHELGIRLFARSTRSLRLTGEGETYLPSAITALDALAQGQ
ERLHGEGNQLQGILQVAAPSDLGRNVLLSCFSQFRKEHPQLTLRLLISDRLANVFRDPVDIAIRYGSAEDASYVERPLAA
WNRRVLVASPAYLSRHGRPAMLEDLSHHCCLLYMLNEHVYDNWKIAQQKIPVKGLLVSDDADVVRLWAIAGEGIAYKSWL
DVREDVLSGRLEILLGQHPGELSPLNLVCPHRKQYSPAVRQLHQMLSRHLAPFAADMAAALANAEIPDSTA
>Mature_311_residues
MMRLGDLKLFVRTVKLGSFSNAAREADLLPGQVSAAIKRLEHELGIRLFARSTRSLRLTGEGETYLPSAITALDALAQGQ
ERLHGEGNQLQGILQVAAPSDLGRNVLLSCFSQFRKEHPQLTLRLLISDRLANVFRDPVDIAIRYGSAEDASYVERPLAA
WNRRVLVASPAYLSRHGRPAMLEDLSHHCCLLYMLNEHVYDNWKIAQQKIPVKGLLVSDDADVVRLWAIAGEGIAYKSWL
DVREDVLSGRLEILLGQHPGELSPLNLVCPHRKQYSPAVRQLHQMLSRHLAPFAADMAAALANAEIPDSTA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=32.4137931034483, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI87081978, Length=294, Percent_Identity=32.6530612244898, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789639, Length=269, Percent_Identity=31.9702602230483, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=32.4232081911263, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1787128, Length=280, Percent_Identity=26.4285714285714, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1789440, Length=261, Percent_Identity=29.1187739463602, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787589, Length=280, Percent_Identity=25.3571428571429, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788706, Length=258, Percent_Identity=29.0697674418605, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=28.5140562248996, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789173, Length=255, Percent_Identity=27.843137254902, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI157672245, Length=178, Percent_Identity=26.4044943820225, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787601, Length=125, Percent_Identity=26.4, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788481, Length=189, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34503; Mature: 34503

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRLGDLKLFVRTVKLGSFSNAAREADLLPGQVSAAIKRLEHELGIRLFARSTRSLRLTG
CCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEEC
EGETYLPSAITALDALAQGQERLHGEGNQLQGILQVAAPSDLGRNVLLSCFSQFRKEHPQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
LTLRLLISDRLANVFRDPVDIAIRYGSAEDASYVERPLAAWNRRVLVASPAYLSRHGRPA
HHHHHHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHCCEEEEECCHHHHCCCCCH
MLEDLSHHCCLLYMLNEHVYDNWKIAQQKIPVKGLLVSDDADVVRLWAIAGEGIAYKSWL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCHHHHH
DVREDVLSGRLEILLGQHPGELSPLNLVCPHRKQYSPAVRQLHQMLSRHLAPFAADMAAA
HHHHHHHCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
LANAEIPDSTA
HHCCCCCCCCC
>Mature Secondary Structure
MMRLGDLKLFVRTVKLGSFSNAAREADLLPGQVSAAIKRLEHELGIRLFARSTRSLRLTG
CCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEEC
EGETYLPSAITALDALAQGQERLHGEGNQLQGILQVAAPSDLGRNVLLSCFSQFRKEHPQ
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
LTLRLLISDRLANVFRDPVDIAIRYGSAEDASYVERPLAAWNRRVLVASPAYLSRHGRPA
HHHHHHHHHHHHHHHCCCHHEEEEECCCCCHHHHHHHHHHHCCEEEEECCHHHHCCCCCH
MLEDLSHHCCLLYMLNEHVYDNWKIAQQKIPVKGLLVSDDADVVRLWAIAGEGIAYKSWL
HHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCEEEEEECCCCCEEEEEEECCCCCCHHHHH
DVREDVLSGRLEILLGQHPGELSPLNLVCPHRKQYSPAVRQLHQMLSRHLAPFAADMAAA
HHHHHHHCCCEEEEECCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
LANAEIPDSTA
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]