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The map label for this gene is scrFIAM [H]

Identifier: 66044681

GI number: 66044681

Start: 1621535

End: 1622950

Strand: Reverse

Name: scrFIAM [H]

Synonym: Psyr_1433

Alternate gene names: 66044681

Gene position: 1622950-1621535 (Counterclockwise)

Preceding gene: 66044682

Following gene: 66044678

Centisome position: 26.63

GC content: 53.6

Gene sequence:

>1416_bases
ATGAATACCTTCTCACTGCTCATCACCCCTAAAATTGGGGAAGCCCGCGGCGTCCCACGTATTTGGCTTGAGGGCCAAAA
ACTATTGAATGCCGGCATCGAAATAGGAACTCGCTTTTCACTCATCCGCCCAGAAGGACAGACCAGGCTGGAACTCGTGC
CTGCCACATCACCAGAGCTCAACACAGGCGATGTCACGGTCTCCAGGCGGGTTAAAAATGGAATCACGACGCCTCTCATC
GAGATCCGGACAGCCCTACTCAGGGGCTTGTTCAAGACCGCAGAGAAAGTCCGAGTTGTCATTCGTTTGGGAAGGATCGT
CATCACCCCCCTCGACAATGACAAGAGGATTGAAGAGCGCCTGGCGCGACTGAAGCGCAAGCTCGATGCACAAGAACCAC
TGGCGGTATGCAGTCTGTTCCACGGTGGAGGCGTGCTGGATCGTGCGATACACGCCGGCCTGGCACGCTCTGGAATTGAT
ACTTTTGTCAGGATTGGTATTGAGGTTGAAGATCAGTACCTGGACGCCAGCTTGCGCAACAACCCGATGCTTTGGCGAGA
CACTTCATTCGCAATCTGTGCAGATGTTCGGGACGTGCAACGCGGCACGGGTACCCCGGAATGTGACTTGGTTGTTGCGG
GCATTCCTTGTACTGGAGCCTCCCGTGCAGGCAAGGCCAAAAACAAACTCAGTTCAGCCGAACAACATCCGTCCGCAGGC
TCGTTATTCATAGACTTTCTGGACTTTGTCCGGTACTCCAACCCAGTCATGGCAATCGTCGAAAATGTACCTGATTACCA
GACCAGCACTTCGATGGAAGTGATCAGATCTGTGTTGAAGTCATTGGGCTACCGGCTGTTCGAGTGCATCCTCGACGGAG
CAGTGTTCGGGGCATTCGAAAGCAGGTCCAGGATGGCTTTGGTTGCATACACGGAGGGGGCTTTTGAGCCTTTGACACTG
GATCAGATCACCCCTCTTCGCACAAAAGAGCGCTCACTCAAGGATCTCATGGACGTTGTGCCAGAAACAGATGAAAGCTG
GAAGTGCTATGACTACCTGGGACGTAAAGAGGAGCGCGACATTGCTAGCGGCAAGGGCTTTCGCAGGCAACTACTGACAC
CTGAAGCAACCTCATGCGGTTGTATTGGGCGTGGATACGCGAAGGCAAGATCGACAGAACCTTTTATTCGACACCCAAGA
AACCCGGCGCTCAGCAGGCTTCTGACGGTAAGCGAACATGCTCGCGTCAAAACCATACCGGTTGAGATCGTTGAAGGAGT
ATCCAACACCACAGCCCACGAGATACTGGGTCAATCTGTCATCTATGCCGCTTTTGAAGCAATTGCGGCCACTGTAGGGA
AGTTGATAACGAAGCTGCACAGAACTGCCAGACCGGCAGGAGTGATGGCGGCTTGA

Upstream 100 bases:

>100_bases
GCGCGAGCAGCCAACTGACGTTGGTGGCTGCACACTCCTCACTTCATCTACCCAATGGGAATCCTCCCATTGGGCCGGGT
TCCCTTTTCGAGAACCCTAC

Downstream 100 bases:

>100_bases
AAACGTCCGGCCTACCTTACGGGTAGCGCCGGGCTATCCTCGAAAGGATTTAGTACCAGCCATATCGCTTGTGGACTTCC
AAATCTGTACTGTGCATGCA

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.ScrFI-A; M.ScrFIA; Cytosine-specific methyltransferase ScrFIA [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MNTFSLLITPKIGEARGVPRIWLEGQKLLNAGIEIGTRFSLIRPEGQTRLELVPATSPELNTGDVTVSRRVKNGITTPLI
EIRTALLRGLFKTAEKVRVVIRLGRIVITPLDNDKRIEERLARLKRKLDAQEPLAVCSLFHGGGVLDRAIHAGLARSGID
TFVRIGIEVEDQYLDASLRNNPMLWRDTSFAICADVRDVQRGTGTPECDLVVAGIPCTGASRAGKAKNKLSSAEQHPSAG
SLFIDFLDFVRYSNPVMAIVENVPDYQTSTSMEVIRSVLKSLGYRLFECILDGAVFGAFESRSRMALVAYTEGAFEPLTL
DQITPLRTKERSLKDLMDVVPETDESWKCYDYLGRKEERDIASGKGFRRQLLTPEATSCGCIGRGYAKARSTEPFIRHPR
NPALSRLLTVSEHARVKTIPVEIVEGVSNTTAHEILGQSVIYAAFEAIAATVGKLITKLHRTARPAGVMAA

Sequences:

>Translated_471_residues
MNTFSLLITPKIGEARGVPRIWLEGQKLLNAGIEIGTRFSLIRPEGQTRLELVPATSPELNTGDVTVSRRVKNGITTPLI
EIRTALLRGLFKTAEKVRVVIRLGRIVITPLDNDKRIEERLARLKRKLDAQEPLAVCSLFHGGGVLDRAIHAGLARSGID
TFVRIGIEVEDQYLDASLRNNPMLWRDTSFAICADVRDVQRGTGTPECDLVVAGIPCTGASRAGKAKNKLSSAEQHPSAG
SLFIDFLDFVRYSNPVMAIVENVPDYQTSTSMEVIRSVLKSLGYRLFECILDGAVFGAFESRSRMALVAYTEGAFEPLTL
DQITPLRTKERSLKDLMDVVPETDESWKCYDYLGRKEERDIASGKGFRRQLLTPEATSCGCIGRGYAKARSTEPFIRHPR
NPALSRLLTVSEHARVKTIPVEIVEGVSNTTAHEILGQSVIYAAFEAIAATVGKLITKLHRTARPAGVMAA
>Mature_471_residues
MNTFSLLITPKIGEARGVPRIWLEGQKLLNAGIEIGTRFSLIRPEGQTRLELVPATSPELNTGDVTVSRRVKNGITTPLI
EIRTALLRGLFKTAEKVRVVIRLGRIVITPLDNDKRIEERLARLKRKLDAQEPLAVCSLFHGGGVLDRAIHAGLARSGID
TFVRIGIEVEDQYLDASLRNNPMLWRDTSFAICADVRDVQRGTGTPECDLVVAGIPCTGASRAGKAKNKLSSAEQHPSAG
SLFIDFLDFVRYSNPVMAIVENVPDYQTSTSMEVIRSVLKSLGYRLFECILDGAVFGAFESRSRMALVAYTEGAFEPLTL
DQITPLRTKERSLKDLMDVVPETDESWKCYDYLGRKEERDIASGKGFRRQLLTPEATSCGCIGRGYAKARSTEPFIRHPR
NPALSRLLTVSEHARVKTIPVEIVEGVSNTTAHEILGQSVIYAAFEAIAATVGKLITKLHRTARPAGVMAA

Specific function: This methylase recognizes the double-stranded sequence CCNGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the ScrFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525
- InterPro:   IPR001387
- InterPro:   IPR010982 [H]

Pfam domain/function: PF00145 DNA_methylase; PF01381 HTH_3 [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 51826; Mature: 51826

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTFSLLITPKIGEARGVPRIWLEGQKLLNAGIEIGTRFSLIRPEGQTRLELVPATSPEL
CCCEEEEEECCCCCCCCCCEEEECCHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCC
NTGDVTVSRRVKNGITTPLIEIRTALLRGLFKTAEKVRVVIRLGRIVITPLDNDKRIEER
CCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
LARLKRKLDAQEPLAVCSLFHGGGVLDRAIHAGLARSGIDTFVRIGIEVEDQYLDASLRN
HHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHEECCEEEHHHHHHHHCC
NPMLWRDTSFAICADVRDVQRGTGTPECDLVVAGIPCTGASRAGKAKNKLSSAEQHPSAG
CCEEEECCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCC
SLFIDFLDFVRYSNPVMAIVENVPDYQTSTSMEVIRSVLKSLGYRLFECILDGAVFGAFE
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SRSRMALVAYTEGAFEPLTLDQITPLRTKERSLKDLMDVVPETDESWKCYDYLGRKEERD
CCCCEEEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHH
IASGKGFRRQLLTPEATSCGCIGRGYAKARSTEPFIRHPRNPALSRLLTVSEHARVKTIP
HHCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHCCCCHHHHHHHHHHCCCEEEEEH
VEIVEGVSNTTAHEILGQSVIYAAFEAIAATVGKLITKLHRTARPAGVMAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNTFSLLITPKIGEARGVPRIWLEGQKLLNAGIEIGTRFSLIRPEGQTRLELVPATSPEL
CCCEEEEEECCCCCCCCCCEEEECCHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCC
NTGDVTVSRRVKNGITTPLIEIRTALLRGLFKTAEKVRVVIRLGRIVITPLDNDKRIEER
CCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHH
LARLKRKLDAQEPLAVCSLFHGGGVLDRAIHAGLARSGIDTFVRIGIEVEDQYLDASLRN
HHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHEECCEEEHHHHHHHHCC
NPMLWRDTSFAICADVRDVQRGTGTPECDLVVAGIPCTGASRAGKAKNKLSSAEQHPSAG
CCEEEECCCEEEEHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHCCCCCC
SLFIDFLDFVRYSNPVMAIVENVPDYQTSTSMEVIRSVLKSLGYRLFECILDGAVFGAFE
HHHHHHHHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SRSRMALVAYTEGAFEPLTLDQITPLRTKERSLKDLMDVVPETDESWKCYDYLGRKEERD
CCCCEEEEEEECCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHH
IASGKGFRRQLLTPEATSCGCIGRGYAKARSTEPFIRHPRNPALSRLLTVSEHARVKTIP
HHCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHHCCCCHHHHHHHHHHCCCEEEEEH
VEIVEGVSNTTAHEILGQSVIYAAFEAIAATVGKLITKLHRTARPAGVMAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8481004 [H]