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The map label for this gene is tolB [H]

Identifier: 66044663

GI number: 66044663

Start: 1600063

End: 1601364

Strand: Direct

Name: tolB [H]

Synonym: Psyr_1415

Alternate gene names: 66044663

Gene position: 1600063-1601364 (Clockwise)

Preceding gene: 66044662

Following gene: 66044664

Centisome position: 26.26

GC content: 56.53

Gene sequence:

>1302_bases
GTGATCAACCTTTTTCGAGGACTGCTGGTCGTTCTCTGTTTCGCATCCGCAATGGTGTCGGCAGAAGAAAAGAACATTCT
GGTCACCAGCGGCAGTGACCGCGCAGCGCCAATCGCTGTTGTGCCTTTCGGATGGCAGGGCGGTAGCGTTCTGCCTGAAG
ACATGGCCGAAATCATAAGCAACGATCTGCGCAACTCCGGTTACTACGCGCCGATCCCGAAGCAGAACATGATCAGCTTG
CCGACCCAGGCCAGCGAAGTCATCTTCCGTGACTGGAAAGCGCTGGGTGCCCAGTACGTCATGGTCGGCAACATCACGCC
TGCCGGCGGACGTCTGCAGATCCAGTACGCGCTGTTCAACGTGGCCACCGAGCAGCAGGTGCTGACCGGTAACGTGTCGG
GTACCAATGATCAGTTGCGCGACATGGCGCACTACATTGCCGACCAGTCGTTCGAGAAGCTCACCGGTATCAAGGGTGCG
TTTTCCACTCGTATGCTTTACGTGACGGCCGAACGCTTCTCGGAAAACAACACGCGTTACACCTTGCAGCGTTCGGATTA
CGACGGTGCTCGTGCAGTAACCCTGTTGCAATCCCGTGAGCCAATCCTGTCGCCGCGTTTTGCGCCGGACGGCAAACGTA
TTGCCTATGTCTCGTTCGAACAGAAGCGTCCACGCATCTTCGTTCAGCACATCGACACCGGTCGTCGCGAACAGATTACC
AACTTCGAAGGCCTGAACGGCGCGCCTGCCTGGTCGCCGGATGGCAGCAAGCTGGCATTCGTACTGTCCAAGGACGGCAA
CCCGGAGATCTATGTAATCAATCTGGCGTCGCGTCAATTGAGCCGCGTGACCAACGATTCTTCGATTGATACTGAACCTT
TCTTCGGCAAGGACGGGTCTACGCTATACTTCACGTCGGACCGTGGCGGCAAGCCACAGATCTACAAGACCAATATCAAT
GGCGGCGGTGCCGAACGCGTGACGTTCGTGGGTAACTACAACGCCAACCCGAAATTGTCAGCCGATGAAAAGACGCTGGT
AATGATTCACCGGCAGGACGGCTTCACTAATTTCAAGGTGGCAGTGCAGGATTTGGCCCGCGGTAGCGTAAAAATCCTCA
CAGATAGCAACCTTGATGAGTCGCCTACTGTTGCGCCCAACGGCACCATGGTAATCTACGCCACCCGCCAGCAGGGCCGG
GGAGTCTTGATGCTCGTGTCCATTAACGGACGCGTAAGGCTCCCGCTTCCTACCGCTCAAGGCGAAGTCAGAGAACCTTC
CTGGTCCCCTTACCTGAACTGA

Upstream 100 bases:

>100_bases
AGAACATTGGACGTTTGACAGAAATGCAGGGTCTGAAGCCGGCTGACTTCGCGCCCTATCGTTCATTCAAGATGACATTC
ACACCTGGAGATCTAGCCTT

Downstream 100 bases:

>100_bases
CGCGGCGCTACACGATTTGCTTAACACACTGGGGTTCATTAGGAGTTTCAAGATGGAAATGCTGAAGTTTGGTAAATTTG
CTGCGCTGGCTCTGGCCATG

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MINLFRGLLVVLCFASAMVSAEEKNILVTSGSDRAAPIAVVPFGWQGGSVLPEDMAEIISNDLRNSGYYAPIPKQNMISL
PTQASEVIFRDWKALGAQYVMVGNITPAGGRLQIQYALFNVATEQQVLTGNVSGTNDQLRDMAHYIADQSFEKLTGIKGA
FSTRMLYVTAERFSENNTRYTLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFVQHIDTGRREQIT
NFEGLNGAPAWSPDGSKLAFVLSKDGNPEIYVINLASRQLSRVTNDSSIDTEPFFGKDGSTLYFTSDRGGKPQIYKTNIN
GGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKVAVQDLARGSVKILTDSNLDESPTVAPNGTMVIYATRQQGR
GVLMLVSINGRVRLPLPTAQGEVREPSWSPYLN

Sequences:

>Translated_433_residues
MINLFRGLLVVLCFASAMVSAEEKNILVTSGSDRAAPIAVVPFGWQGGSVLPEDMAEIISNDLRNSGYYAPIPKQNMISL
PTQASEVIFRDWKALGAQYVMVGNITPAGGRLQIQYALFNVATEQQVLTGNVSGTNDQLRDMAHYIADQSFEKLTGIKGA
FSTRMLYVTAERFSENNTRYTLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFVQHIDTGRREQIT
NFEGLNGAPAWSPDGSKLAFVLSKDGNPEIYVINLASRQLSRVTNDSSIDTEPFFGKDGSTLYFTSDRGGKPQIYKTNIN
GGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKVAVQDLARGSVKILTDSNLDESPTVAPNGTMVIYATRQQGR
GVLMLVSINGRVRLPLPTAQGEVREPSWSPYLN
>Mature_433_residues
MINLFRGLLVVLCFASAMVSAEEKNILVTSGSDRAAPIAVVPFGWQGGSVLPEDMAEIISNDLRNSGYYAPIPKQNMISL
PTQASEVIFRDWKALGAQYVMVGNITPAGGRLQIQYALFNVATEQQVLTGNVSGTNDQLRDMAHYIADQSFEKLTGIKGA
FSTRMLYVTAERFSENNTRYTLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFVQHIDTGRREQIT
NFEGLNGAPAWSPDGSKLAFVLSKDGNPEIYVINLASRQLSRVTNDSSIDTEPFFGKDGSTLYFTSDRGGKPQIYKTNIN
GGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKVAVQDLARGSVKILTDSNLDESPTVAPNGTMVIYATRQQGR
GVLMLVSINGRVRLPLPTAQGEVREPSWSPYLN

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=435, Percent_Identity=42.9885057471264, Blast_Score=335, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47697; Mature: 47697

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLFRGLLVVLCFASAMVSAEEKNILVTSGSDRAAPIAVVPFGWQGGSVLPEDMAEIIS
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
NDLRNSGYYAPIPKQNMISLPTQASEVIFRDWKALGAQYVMVGNITPAGGRLQIQYALFN
HHHHCCCEECCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEE
VATEQQVLTGNVSGTNDQLRDMAHYIADQSFEKLTGIKGAFSTRMLYVTAERFSENNTRY
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEEEHHCCCCCEE
TLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFVQHIDTGRREQIT
EEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHCC
NFEGLNGAPAWSPDGSKLAFVLSKDGNPEIYVINLASRQLSRVTNDSSIDTEPFFGKDGS
CCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCC
TLYFTSDRGGKPQIYKTNINGGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKV
EEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHH
AVQDLARGSVKILTDSNLDESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTAQ
HHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCEEEECCCCCC
GEVREPSWSPYLN
CCCCCCCCCCCCC
>Mature Secondary Structure
MINLFRGLLVVLCFASAMVSAEEKNILVTSGSDRAAPIAVVPFGWQGGSVLPEDMAEIIS
CCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHHHHH
NDLRNSGYYAPIPKQNMISLPTQASEVIFRDWKALGAQYVMVGNITPAGGRLQIQYALFN
HHHHCCCEECCCCCCCEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEEEEE
VATEQQVLTGNVSGTNDQLRDMAHYIADQSFEKLTGIKGAFSTRMLYVTAERFSENNTRY
CCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEEEEEHHCCCCCEE
TLQRSDYDGARAVTLLQSREPILSPRFAPDGKRIAYVSFEQKRPRIFVQHIDTGRREQIT
EEEECCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHCC
NFEGLNGAPAWSPDGSKLAFVLSKDGNPEIYVINLASRQLSRVTNDSSIDTEPFFGKDGS
CCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCCCCC
TLYFTSDRGGKPQIYKTNINGGGAERVTFVGNYNANPKLSADEKTLVMIHRQDGFTNFKV
EEEEECCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCHHH
AVQDLARGSVKILTDSNLDESPTVAPNGTMVIYATRQQGRGVLMLVSINGRVRLPLPTAQ
HHHHHCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCEEEECCCCCC
GEVREPSWSPYLN
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA