Click here to switch to the map view.

The map label for this gene is eno [H]

Identifier: 66044611

GI number: 66044611

Start: 1546984

End: 1548270

Strand: Direct

Name: eno [H]

Synonym: Psyr_1363

Alternate gene names: 66044611

Gene position: 1546984-1548270 (Clockwise)

Preceding gene: 66044610

Following gene: 66044612

Centisome position: 25.39

GC content: 58.97

Gene sequence:

>1287_bases
ATGGCAAAAATCGTTGACATCAAAGGTCGTGAAGTTCTTGACTCCCGTGGCAACCCAACCGTTGAAGCAGATGTGCTCCT
CGATAACGGCATCATCGGCAGCGCCTGCGCGCCGTCTGGCGCTTCAACCGGCTCGCGCGAAGCGCTGGAGCTGCGTGATG
GCGACAAGAGCCGTTACATGGGCAAGGGCGTTCTGAAGGCTGTTGCCAATATCAATGGTCCGATCCGCGACCTGCTGCTG
GGCAAGGATCCGGTTGACCAGAAGGCCCTGGATCACGCGATGATCGAGCTGGACGGCACCGAAAACAAGGCAAGCCTGGG
CGCGAACGCCATCCTCGCGGTCTCCCTGGCAGCTGCCAAGGCAGCGGCACAGGATCAGGACCTGCCGCTGTACGCGCACA
TCGCCAACCTCAATGGCACACCGGGCGTGTATTCCATGCCGGTGCCGATGATGAACATCATCAACGGTGGCGAGCATGCC
GATAACAACATCGACATTCAGGAATTCATGATCCAGCCTGTCGGCGCCAAGTCCTTCGCTGAAGGCCTGCGCTGGGGCAC
CGAGATTTTCCATCACCTCAAGGCGGTTCTCAAGGCGCGTGGCCTGAACACCGCAGTGGGCGACGAAGGTGGCTTCGCGC
CTAACCTGGCGTCCAACAAGGAAGCGCTCGACGCCATCGCCGAAGCGGTTGCCAATGCGGGTTACACGCTGGGCACTGAC
GTGACGCTGGCGCTGGACTGCGCAGCGAGCGAGTTCTACAAGAACGGCAAGTACAAACTGAGCGAAGAGGGCGAGTACAG
CTCTGCCGAGTTTGCCGAATACCTTGCCGAGCTGACTCGCAAGCACCCGATCATTTCCATCGAAGACGGTCTGGACGAGT
CCGACTGGGATGGCTGGAAAATCCTCACCGACAAGATCGGCGAGAAAACCCAGCTGGTAGGTGACGACCTGTTCGTGACC
AACACCAAGATCCTCAAGGAAGGCATCGACAAGAAGATCGCCAACTCGATCCTGATCAAGTTCAACCAGATCGGCACGCT
GACCGAAACTCTGGAAGCCATTCAGATGGCCAAGGCTGCCGGTTATACTGCGATCATCTCTCACCGTTCCGGCGAGACCG
AAGATTCGACCATTGCCGACCTCGCGGTGGGCACCTCTGCCGGTCAGATCAAAACCGGTTCGCTGTGCCGCTCCGATCGC
GTGTCCAAGTACAACCAATTGCTGCGCATCGAGGAGCAATTGGGCTCCAAGGCTGTGTATCGTGGTCGTGCCGAGTTTCG
CGGCTGA

Upstream 100 bases:

>100_bases
TGACGGGGTTGTCACATTCAGGGCTGCCGGTTTTTGCGCCTGACTGTGAACATCGTTTTCCCGCTGCGTCGTTTTCGTCA
ATCCTGGAGTGTTTACAACA

Downstream 100 bases:

>100_bases
GCCATAGATGGTAAAAAGACGCAGAGTGCTGCACGGATTCGGGTTCCCTGACTCGCCTGTGCAGCTCCGATGCTGACGCT
CCGCGGTAATCTGGAGTGAT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYMGKGVLKAVANINGPIRDLLL
GKDPVDQKALDHAMIELDGTENKASLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHA
DNNIDIQEFMIQPVGAKSFAEGLRWGTEIFHHLKAVLKARGLNTAVGDEGGFAPNLASNKEALDAIAEAVANAGYTLGTD
VTLALDCAASEFYKNGKYKLSEEGEYSSAEFAEYLAELTRKHPIISIEDGLDESDWDGWKILTDKIGEKTQLVGDDLFVT
NTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAAGYTAIISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDR
VSKYNQLLRIEEQLGSKAVYRGRAEFRG

Sequences:

>Translated_428_residues
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYMGKGVLKAVANINGPIRDLLL
GKDPVDQKALDHAMIELDGTENKASLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHA
DNNIDIQEFMIQPVGAKSFAEGLRWGTEIFHHLKAVLKARGLNTAVGDEGGFAPNLASNKEALDAIAEAVANAGYTLGTD
VTLALDCAASEFYKNGKYKLSEEGEYSSAEFAEYLAELTRKHPIISIEDGLDESDWDGWKILTDKIGEKTQLVGDDLFVT
NTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAAGYTAIISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDR
VSKYNQLLRIEEQLGSKAVYRGRAEFRG
>Mature_427_residues
AKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYMGKGVLKAVANINGPIRDLLLG
KDPVDQKALDHAMIELDGTENKASLGANAILAVSLAAAKAAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHAD
NNIDIQEFMIQPVGAKSFAEGLRWGTEIFHHLKAVLKARGLNTAVGDEGGFAPNLASNKEALDAIAEAVANAGYTLGTDV
TLALDCAASEFYKNGKYKLSEEGEYSSAEFAEYLAELTRKHPIISIEDGLDESDWDGWKILTDKIGEKTQLVGDDLFVTN
TKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAAGYTAIISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDRV
SKYNQLLRIEEQLGSKAVYRGRAEFRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=53.1322505800464, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=53.1322505800464, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=51.8433179723502, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=51.3824884792627, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=47.5524475524476, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=28.955223880597, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=28.955223880597, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=28.955223880597, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI310129182, Length=206, Percent_Identity=26.2135922330097, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310110045, Length=206, Percent_Identity=26.2135922330097, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310120572, Length=206, Percent_Identity=26.2135922330097, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=73.7209302325581, Blast_Score=640, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71995829, Length=435, Percent_Identity=54.2528735632184, Blast_Score=443, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=54.2528735632184, Blast_Score=442, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI32563855, Length=194, Percent_Identity=49.4845360824742, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6321693, Length=437, Percent_Identity=49.4279176201373, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=48.8425925925926, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=48.8425925925926, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=48.8425925925926, Blast_Score=390, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321968, Length=431, Percent_Identity=49.4199535962877, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=52.3148148148148, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=52.3148148148148, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=52.3148148148148, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=52.3148148148148, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=52.3148148148148, Blast_Score=413, Evalue=1e-116,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=52.3148148148148, Blast_Score=413, Evalue=1e-116,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 45682; Mature: 45551

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYM
CCEEEECCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVANINGPIRDLLLGKDPVDQKALDHAMIELDGTENKASLGANAILAVSLAAAK
HHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHEEEEECCCCCCHHCCCCHHHHHHHHHHH
AAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHADNNIDIQEFMIQPVGAKSFA
HHCCCCCCCEEEEEECCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EGLRWGTEIFHHLKAVLKARGLNTAVGDEGGFAPNLASNKEALDAIAEAVANAGYTLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLALDCAASEFYKNGKYKLSEEGEYSSAEFAEYLAELTRKHPIISIEDGLDESDWDGWK
EEEEEEHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEE
ILTDKIGEKTQLVGDDLFVTNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAA
EEHHHCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHHHHC
GYTAIISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDRVSKYNQLLRIEEQLGSKAVY
CCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
RGRAEFRG
CCCHHCCC
>Mature Secondary Structure 
AKIVDIKGREVLDSRGNPTVEADVLLDNGIIGSACAPSGASTGSREALELRDGDKSRYM
CEEEECCCHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
GKGVLKAVANINGPIRDLLLGKDPVDQKALDHAMIELDGTENKASLGANAILAVSLAAAK
HHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHEEEEECCCCCCHHCCCCHHHHHHHHHHH
AAAQDQDLPLYAHIANLNGTPGVYSMPVPMMNIINGGEHADNNIDIQEFMIQPVGAKSFA
HHCCCCCCCEEEEEECCCCCCCEEECCCHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
EGLRWGTEIFHHLKAVLKARGLNTAVGDEGGFAPNLASNKEALDAIAEAVANAGYTLGTD
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEECCC
VTLALDCAASEFYKNGKYKLSEEGEYSSAEFAEYLAELTRKHPIISIEDGLDESDWDGWK
EEEEEEHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEE
ILTDKIGEKTQLVGDDLFVTNTKILKEGIDKKIANSILIKFNQIGTLTETLEAIQMAKAA
EEHHHCCCHHHHCCCCEEEEHHHHHHHHHHHHHHHHHEEEEHHCCCHHHHHHHHHHHHHC
GYTAIISHRSGETEDSTIADLAVGTSAGQIKTGSLCRSDRVSKYNQLLRIEEQLGSKAVY
CCEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
RGRAEFRG
CCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA