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The map label for this gene is oprF [H]

Identifier: 66044564

GI number: 66044564

Start: 1493517

End: 1494299

Strand: Reverse

Name: oprF [H]

Synonym: Psyr_1316

Alternate gene names: 66044564

Gene position: 1494299-1493517 (Counterclockwise)

Preceding gene: 66044565

Following gene: 66044550

Centisome position: 24.52

GC content: 58.88

Gene sequence:

>783_bases
ATGCGTAAACAATTGATGATCCCTGCTCTGCTGGCCATGAGCGTTGCCCTGGCAGCCTGTGCAACCAAGCCGAACCCTAA
CCTGGAACAGGCTCGCAGCAACTTCACCGCGCTGCAAACCAACCCTCAGGCCACTCAAGTCGCAGCCCTTGAAACCAAGG
ACGCCAGCGAATGGCTGACCAAGGCAGAACAGGCCTTCCGCAACGATGAGGACGTGCAGAAAGTCGACCAACTGTCGTAT
CTGACCAACCAGCGCGTCGAGCTGGCCAAGCAGACCATCGCCCTGCGCACTTCTGAAGCAGCGCTGCAAAATGCTTCTGC
CGACCGCGCCAAGGCCCGTCTGGAAGCACGTGACGCGCAGATCGCTGCACTGAAAAACAGCCTGAACGCCAAGCAGACCG
AACGCGGTACGCTGGTGACCTTCGGTGACGTGCTGTTCGACTACAACAAGGCCGAACTGAAGCCGACCGCCCAGGGCGAC
ATTGGCAAGCTGGCTGCCTTCCTGCAGGAAAACCCTGATCGCAAAGTGATTGTCGAAGGCTACACCGACAGCACCGGTTC
GGCTTCCTACAACCAGTCGCTGTCCGAGCGTCGCGCCAACTCGGTGCGTATGGCTCTGGTACGCATGGGTGTAGACCCAG
CGCGCGTTGTGACCATGGGTTATGGCAAGGAATACCCGGTTGCTGACAACACCAGCAACTCGGGCCGCGCCATGAACCGT
CGTGTGGAAGTGACTATCTCCAACGACAACCAGCCTGTTGCACCGCGCTCTTCGATGAAATAA

Upstream 100 bases:

>100_bases
TCAACGAACTCCGTGAAGAAGGCCTGCGTCAGGTGCAGTGATTGCCCTGTACACCCTTCTGTTTTCATGAATTCGTAACT
GATTAAAGGACGACCCTATT

Downstream 100 bases:

>100_bases
GCGACTGAGCTGCTGCACCGAAGCCCCGCCTGCGCATACAGGCGGGGCTTTTTCATGGACGCTGGTTTTTACCGGTGTCT
TACGGCCGAGGCGTTTGCTG

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRKQLMIPALLAMSVALAACATKPNPNLEQARSNFTALQTNPQATQVAALETKDASEWLTKAEQAFRNDEDVQKVDQLSY
LTNQRVELAKQTIALRTSEAALQNASADRAKARLEARDAQIAALKNSLNAKQTERGTLVTFGDVLFDYNKAELKPTAQGD
IGKLAAFLQENPDRKVIVEGYTDSTGSASYNQSLSERRANSVRMALVRMGVDPARVVTMGYGKEYPVADNTSNSGRAMNR
RVEVTISNDNQPVAPRSSMK

Sequences:

>Translated_260_residues
MRKQLMIPALLAMSVALAACATKPNPNLEQARSNFTALQTNPQATQVAALETKDASEWLTKAEQAFRNDEDVQKVDQLSY
LTNQRVELAKQTIALRTSEAALQNASADRAKARLEARDAQIAALKNSLNAKQTERGTLVTFGDVLFDYNKAELKPTAQGD
IGKLAAFLQENPDRKVIVEGYTDSTGSASYNQSLSERRANSVRMALVRMGVDPARVVTMGYGKEYPVADNTSNSGRAMNR
RVEVTISNDNQPVAPRSSMK
>Mature_260_residues
MRKQLMIPALLAMSVALAACATKPNPNLEQARSNFTALQTNPQATQVAALETKDASEWLTKAEQAFRNDEDVQKVDQLSY
LTNQRVELAKQTIALRTSEAALQNASADRAKARLEARDAQIAALKNSLNAKQTERGTLVTFGDVLFDYNKAELKPTAQGD
IGKLAAFLQENPDRKVIVEGYTDSTGSASYNQSLSERRANSVRMALVRMGVDPARVVTMGYGKEYPVADNTSNSGRAMNR
RVEVTISNDNQPVAPRSSMK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=105, Percent_Identity=45.7142857142857, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787191, Length=126, Percent_Identity=40.4761904761905, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788957, Length=105, Percent_Identity=40.9523809523809, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786962, Length=98, Percent_Identity=38.7755102040816, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 28438; Mature: 28438

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKQLMIPALLAMSVALAACATKPNPNLEQARSNFTALQTNPQATQVAALETKDASEWLT
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCHHEEEEECCCCHHHHHH
KAEQAFRNDEDVQKVDQLSYLTNQRVELAKQTIALRTSEAALQNASADRAKARLEARDAQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IAALKNSLNAKQTERGTLVTFGDVLFDYNKAELKPTAQGDIGKLAAFLQENPDRKVIVEG
HHHHHHHCCCCCCCCCCEEEEHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
YTDSTGSASYNQSLSERRANSVRMALVRMGVDPARVVTMGYGKEYPVADNTSNSGRAMNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCCCCEECE
RVEVTISNDNQPVAPRSSMK
EEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRKQLMIPALLAMSVALAACATKPNPNLEQARSNFTALQTNPQATQVAALETKDASEWLT
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECCCCHHEEEEECCCCHHHHHH
KAEQAFRNDEDVQKVDQLSYLTNQRVELAKQTIALRTSEAALQNASADRAKARLEARDAQ
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
IAALKNSLNAKQTERGTLVTFGDVLFDYNKAELKPTAQGDIGKLAAFLQENPDRKVIVEG
HHHHHHHCCCCCCCCCCEEEEHHHHHCCCHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEC
YTDSTGSASYNQSLSERRANSVRMALVRMGVDPARVVTMGYGKEYPVADNTSNSGRAMNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCCCCCEECE
RVEVTISNDNQPVAPRSSMK
EEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]