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The map label for this gene is hisS [H]

Identifier: 66044497

GI number: 66044497

Start: 1404477

End: 1405766

Strand: Direct

Name: hisS [H]

Synonym: Psyr_1249

Alternate gene names: 66044497

Gene position: 1404477-1405766 (Clockwise)

Preceding gene: 66044496

Following gene: 66044498

Centisome position: 23.05

GC content: 60.7

Gene sequence:

>1290_bases
GTGAGTAAGTCTCTACAAGCCATTCGTGGCATGAACGACATTCTGCCCGAGCAGACGCCGCTGTGGCGTCACTTCGAAGG
CACCGTTGCCCGTCTGCTGGATAACTACGGTTACCGGCAGATTCGTATGCCTATCGTCGAATTCACCGAGCTGTTCAAGC
GCTCCATCGGTGAAGTGACCGACATCGTCGAGAAAGAGATGTACACCTTCGCCGACCGCAACGGCGACTCTCTGACCCTG
CGCCCTGAAGGCACTGCCGCGTGCGTGCGTGCGGTCCTCGAGCACGGCATCACTGGCGGCGGCCAGGTTCAGAAACTGTG
GTACATCGGTCCGATGTTCCGTCACGAGCGTCCGCAGAAAGGCCGTTATCGTCAGTTTCACCAGATCGGTGTCGAGGTCT
TCAACCTGGACGGTCCGGATATCGATGCCGAACTGATTGTCATGACCTGGCGCCTGTGGGGCCTGCTGGGCATTCGCGAC
GCCGTCAAGCTCGAGCTGAACAGCCTGGGCACCAGCGAGGCGCGTGCGCGTTATCGCGAGGCGCTGGTCGAATACCTCTC
GGCACGTCTCGACCAGCTGGACGAAGACAGCCAGCGCCGACTGAAAACCAACCCGCTTCGCGTGCTGGACACCAAGCACC
CTGAAACCCAGGCTGTGCTGGTCGATGCGCCGAAACTGGCGGACTATCTGGATGACGAATCCCGCGTTCACTTCGAAGGC
CTGAAGGCCCGTCTGGATGCCGCCGGTATTCCTTATGTGATCAATCCCAAGCTGGTGCGTGGCCTGGATTACTACAGCAA
GACCGTTTTCGAGTGGGTCACCGACCAACTCGGTGCCCAGGGTACGGTCTGTGCGGGCGGTCGTTACGACGGTCTGGTCG
AGCAGATGGGCGGCAAGCCTACTGCAGGCGTCGGTTTCGCGATGGGCATCGAGCGACTGGTGCTGCTGCTCGAAACCCTC
GAGCAGGTGCCGGAAGAGATTGCCCGTCAGGTCGATGTCTACCTGTGCGCATTTGGCGAAGCCGCCGAGCTGGCTGCGCT
GGCACTGACCGAAAAGGTGCGTGATCAGCTGCCGACTCTGCGGTTGCAGGTCAACGCCGGTGCCGGCAGCTTCAAAAGCC
AGTTCAAGAAAGCCGACAAGAGCGGTGCGCTGTACGCGCTGATCCTCGGCGAAGAAGAACTGGCGGCGAAGGTCATTGGC
GTCAAACCCCTGCGCGGTCAGGGTGAACAACAAAATATTGCCTGGGATGCTCTGTCGGAGCACCTGGCCTCCTGCGTCGT
GCAGGGTTGA

Upstream 100 bases:

>100_bases
AATGAGCTGGAACGCTTGATTCGCGAGAAAGCGGCTGAAAAAGCCGAAGCCGACGCCTCGGTCATCGTGCGCGGCTAACC
CGGATTGCTAAGGATTTTTT

Downstream 100 bases:

>100_bases
AGCTGATTTAACAGCCGTTTAGCGAATAGGAGTATTGGGGTGTCGGGTACCGAAGATGAAGAGCTGGCGGTAATGAAGGA
CTGGTGGCAGCGCAACGGCA

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MSKSLQAIRGMNDILPEQTPLWRHFEGTVARLLDNYGYRQIRMPIVEFTELFKRSIGEVTDIVEKEMYTFADRNGDSLTL
RPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQKGRYRQFHQIGVEVFNLDGPDIDAELIVMTWRLWGLLGIRD
AVKLELNSLGTSEARARYREALVEYLSARLDQLDEDSQRRLKTNPLRVLDTKHPETQAVLVDAPKLADYLDDESRVHFEG
LKARLDAAGIPYVINPKLVRGLDYYSKTVFEWVTDQLGAQGTVCAGGRYDGLVEQMGGKPTAGVGFAMGIERLVLLLETL
EQVPEEIARQVDVYLCAFGEAAELAALALTEKVRDQLPTLRLQVNAGAGSFKSQFKKADKSGALYALILGEEELAAKVIG
VKPLRGQGEQQNIAWDALSEHLASCVVQG

Sequences:

>Translated_429_residues
MSKSLQAIRGMNDILPEQTPLWRHFEGTVARLLDNYGYRQIRMPIVEFTELFKRSIGEVTDIVEKEMYTFADRNGDSLTL
RPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQKGRYRQFHQIGVEVFNLDGPDIDAELIVMTWRLWGLLGIRD
AVKLELNSLGTSEARARYREALVEYLSARLDQLDEDSQRRLKTNPLRVLDTKHPETQAVLVDAPKLADYLDDESRVHFEG
LKARLDAAGIPYVINPKLVRGLDYYSKTVFEWVTDQLGAQGTVCAGGRYDGLVEQMGGKPTAGVGFAMGIERLVLLLETL
EQVPEEIARQVDVYLCAFGEAAELAALALTEKVRDQLPTLRLQVNAGAGSFKSQFKKADKSGALYALILGEEELAAKVIG
VKPLRGQGEQQNIAWDALSEHLASCVVQG
>Mature_428_residues
SKSLQAIRGMNDILPEQTPLWRHFEGTVARLLDNYGYRQIRMPIVEFTELFKRSIGEVTDIVEKEMYTFADRNGDSLTLR
PEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQKGRYRQFHQIGVEVFNLDGPDIDAELIVMTWRLWGLLGIRDA
VKLELNSLGTSEARARYREALVEYLSARLDQLDEDSQRRLKTNPLRVLDTKHPETQAVLVDAPKLADYLDDESRVHFEGL
KARLDAAGIPYVINPKLVRGLDYYSKTVFEWVTDQLGAQGTVCAGGRYDGLVEQMGGKPTAGVGFAMGIERLVLLLETLE
QVPEEIARQVDVYLCAFGEAAELAALALTEKVRDQLPTLRLQVNAGAGSFKSQFKKADKSGALYALILGEEELAAKVIGV
KPLRGQGEQQNIAWDALSEHLASCVVQG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=63.1205673758865, Blast_Score=539, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 47829; Mature: 47698

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSLQAIRGMNDILPEQTPLWRHFEGTVARLLDNYGYRQIRMPIVEFTELFKRSIGEVT
CCCHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
DIVEKEMYTFADRNGDSLTLRPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQK
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCC
GRYRQFHQIGVEVFNLDGPDIDAELIVMTWRLWGLLGIRDAVKLELNSLGTSEARARYRE
CHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
ALVEYLSARLDQLDEDSQRRLKTNPLRVLDTKHPETQAVLVDAPKLADYLDDESRVHFEG
HHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCEEEEECHHHHHHCCCCCCCHHHH
LKARLDAAGIPYVINPKLVRGLDYYSKTVFEWVTDQLGAQGTVCAGGRYDGLVEQMGGKP
HHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCC
TAGVGFAMGIERLVLLLETLEQVPEEIARQVDVYLCAFGEAAELAALALTEKVRDQLPTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCEE
RLQVNAGAGSFKSQFKKADKSGALYALILGEEELAAKVIGVKPLRGQGEQQNIAWDALSE
EEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
HLASCVVQG
HHHHHHCCC
>Mature Secondary Structure 
SKSLQAIRGMNDILPEQTPLWRHFEGTVARLLDNYGYRQIRMPIVEFTELFKRSIGEVT
CCHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHH
DIVEKEMYTFADRNGDSLTLRPEGTAACVRAVLEHGITGGGQVQKLWYIGPMFRHERPQK
HHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEECCHHHCCCCCC
GRYRQFHQIGVEVFNLDGPDIDAELIVMTWRLWGLLGIRDAVKLELNSLGTSEARARYRE
CHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHH
ALVEYLSARLDQLDEDSQRRLKTNPLRVLDTKHPETQAVLVDAPKLADYLDDESRVHFEG
HHHHHHHHHHHHCCCHHHHHHCCCCEEEEECCCCCCCEEEEECHHHHHHCCCCCCCHHHH
LKARLDAAGIPYVINPKLVRGLDYYSKTVFEWVTDQLGAQGTVCAGGRYDGLVEQMGGKP
HHHHHCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHCCCC
TAGVGFAMGIERLVLLLETLEQVPEEIARQVDVYLCAFGEAAELAALALTEKVRDQLPTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHCCCEE
RLQVNAGAGSFKSQFKKADKSGALYALILGEEELAAKVIGVKPLRGQGEQQNIAWDALSE
EEEEECCCCHHHHHHHHCCCCCCEEEEEECCHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
HLASCVVQG
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA