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The map label for this gene is gcvH [H]

Identifier: 66044348

GI number: 66044348

Start: 1241475

End: 1241858

Strand: Reverse

Name: gcvH [H]

Synonym: Psyr_1097

Alternate gene names: 66044348

Gene position: 1241858-1241475 (Counterclockwise)

Preceding gene: 66044349

Following gene: 66044347

Centisome position: 20.38

GC content: 60.16

Gene sequence:

>384_bases
ATGAGCGATTTGCGATTCACCGTTGACCATGAATGGCTGCGTGCCGAAACCGACGGCAGCGTGACGGTCGGCATTACGCC
CTACGCACAGGAATCGCTGGGCGATGTGGTCTTCGTGCAGCTCCCGGAATTACAGGCCTACACTCAACACGCTGAAGTGT
CGGTGGTGGAATCGGTGAAGGCCGCCAGCAGCATCAACATGCCGCTGGACGGTGACGTCGTCGAGGTCAATTCCGCCCTC
GATGCCACGCCGGAGCTGGTCAATGAAGATGCCCTCGGTGCGGGCTGGTTCTTTCGCTTCGTTCCACAAGACGCCAACGC
CATTCACGGCCTGCTGGATCAGGACGCCTACGACCGCCTGATCAAAGCCAACGCCGAAGCCTGA

Upstream 100 bases:

>100_bases
CATTGATTTAAAAAGAAAAATAAAACATGGCCGCTAAATTGCTAGGTCATGGCTCATATTGTTCGCGTGCTGCGCGAGCC
CTGTTCTCCGAGGAGTTTGT

Downstream 100 bases:

>100_bases
GGAGCACGCCATGACTGACCGTATCGAACTGACCACTGCCAACGAATTCATTGCCCGTCACATTGGCCCGCGCGCGGCTG
ACGAGCTGGCCATGCTGCAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 127; Mature: 126

Protein sequence:

>127_residues
MSDLRFTVDHEWLRAETDGSVTVGITPYAQESLGDVVFVQLPELQAYTQHAEVSVVESVKAASSINMPLDGDVVEVNSAL
DATPELVNEDALGAGWFFRFVPQDANAIHGLLDQDAYDRLIKANAEA

Sequences:

>Translated_127_residues
MSDLRFTVDHEWLRAETDGSVTVGITPYAQESLGDVVFVQLPELQAYTQHAEVSVVESVKAASSINMPLDGDVVEVNSAL
DATPELVNEDALGAGWFFRFVPQDANAIHGLLDQDAYDRLIKANAEA
>Mature_126_residues
SDLRFTVDHEWLRAETDGSVTVGITPYAQESLGDVVFVQLPELQAYTQHAEVSVVESVKAASSINMPLDGDVVEVNSALD
ATPELVNEDALGAGWFFRFVPQDANAIHGLLDQDAYDRLIKANAEA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=124, Percent_Identity=40.3225806451613, Blast_Score=98, Evalue=2e-21,
Organism=Homo sapiens, GI49574537, Length=124, Percent_Identity=40.3225806451613, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=47.5409836065574, Blast_Score=118, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=38.135593220339, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17551294, Length=124, Percent_Identity=38.7096774193548, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319272, Length=122, Percent_Identity=38.5245901639344, Blast_Score=93, Evalue=1e-20,
Organism=Drosophila melanogaster, GI17865652, Length=123, Percent_Identity=38.2113821138211, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13805; Mature: 13674

Theoretical pI: Translated: 3.86; Mature: 3.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLRFTVDHEWLRAETDGSVTVGITPYAQESLGDVVFVQLPELQAYTQHAEVSVVESVK
CCCCEEEECCCEEEECCCCCEEEEECCCCHHCCCCEEEEECCCHHHHHHCCHHHHHHHHH
AASSINMPLDGDVVEVNSALDATPELVNEDALGAGWFFRFVPQDANAIHGLLDQDAYDRL
HHHCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEECCCCCHHHHHHCCCHHHHHH
IKANAEA
HHCCCCC
>Mature Secondary Structure 
SDLRFTVDHEWLRAETDGSVTVGITPYAQESLGDVVFVQLPELQAYTQHAEVSVVESVK
CCCEEEECCCEEEECCCCCEEEEECCCCHHCCCCEEEEECCCHHHHHHCCHHHHHHHHH
AASSINMPLDGDVVEVNSALDATPELVNEDALGAGWFFRFVPQDANAIHGLLDQDAYDRL
HHHCCCCCCCCCEEEECCCCCCCHHHHCCCCCCCCEEEEECCCCCHHHHHHCCCHHHHHH
IKANAEA
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]