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The map label for this gene is rutF

Identifier: 66044248

GI number: 66044248

Start: 1129873

End: 1130409

Strand: Reverse

Name: rutF

Synonym: Psyr_0995

Alternate gene names: 66044248

Gene position: 1130409-1129873 (Counterclockwise)

Preceding gene: 66044249

Following gene: 66044247

Centisome position: 18.55

GC content: 63.87

Gene sequence:

>537_bases
ATGCCTACCTCCTCTGCAACTCCCGCCATTCTGCCCATCGCCGCTGTCAGCCAGCTGGCGTTTCGCGATGCGATGTCCGG
CCTGGCCGCCGCCGTCAACCTCATCACCACCGACGGCCCTGGCGGACGCGCCGGCTTTACCGCGACCGCCGTGTGCAGCG
TGACGGATCAACCGCCGACGCTGCTGGTCTGTATCAACCGCAGTGCGTCGGTGTATGACGCCTTCATCGAGAACGGCACG
TTGTGCGTGAATACGCTGGGCAACGGTCAGCAGGACTTGTCCAACCTGTTCGGCGGCAAGAGCTCCCAGCAGGAGCGCTT
TGCCGGCGGGCAGTGGGAGGCCGGAGTGACCGGCGCACCGGTCCTGGACAGTGCAAAACTGGCGCTCGACTGCAAAGTGA
GCCAGTCAGTCAGCGTCGGCACGCATGACATTCTGTTCTGCGAGGTGGTGGATATCAGGCATCAGAGCGGCGCAGATGCA
TTGGTCTACTTCGGCCGTCGCTATCATCACCTGCCGAGCGAAACGCCAGCGGCCTGA

Upstream 100 bases:

>100_bases
GCCATGCCTCTAACATTACCGATCCACTGCCCTTCCAACGCGCCCTGCGCGCTTTTCTCAGCACACAACCCTGACACACA
ACCCTGAAAGGACTCTTCCA

Downstream 100 bases:

>100_bases
GGGCTTTGCCGGTTGACTGGCAGACTTCCAGAATGAACCAGCCAGAATGAGCCCGCCGGAATGTACCAAATAGACATGAT
TTTTTGCGACAGCTCCGTAT

Product: flavin reductase-like

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase

Number of amino acids: Translated: 178; Mature: 177

Protein sequence:

>178_residues
MPTSSATPAILPIAAVSQLAFRDAMSGLAAAVNLITTDGPGGRAGFTATAVCSVTDQPPTLLVCINRSASVYDAFIENGT
LCVNTLGNGQQDLSNLFGGKSSQQERFAGGQWEAGVTGAPVLDSAKLALDCKVSQSVSVGTHDILFCEVVDIRHQSGADA
LVYFGRRYHHLPSETPAA

Sequences:

>Translated_178_residues
MPTSSATPAILPIAAVSQLAFRDAMSGLAAAVNLITTDGPGGRAGFTATAVCSVTDQPPTLLVCINRSASVYDAFIENGT
LCVNTLGNGQQDLSNLFGGKSSQQERFAGGQWEAGVTGAPVLDSAKLALDCKVSQSVSVGTHDILFCEVVDIRHQSGADA
LVYFGRRYHHLPSETPAA
>Mature_177_residues
PTSSATPAILPIAAVSQLAFRDAMSGLAAAVNLITTDGPGGRAGFTATAVCSVTDQPPTLLVCINRSASVYDAFIENGTL
CVNTLGNGQQDLSNLFGGKSSQQERFAGGQWEAGVTGAPVLDSAKLALDCKVSQSVSVGTHDILFCEVVDIRHQSGADAL
VYFGRRYHHLPSETPAA

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily

Homologues:

Organism=Escherichia coli, GI87081819, Length=156, Percent_Identity=63.4615384615385, Blast_Score=208, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTF_PSEU2 (Q4ZXS1)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_234089.1
- ProteinModelPortal:   Q4ZXS1
- SMR:   Q4ZXS1
- STRING:   Q4ZXS1
- GeneID:   3366489
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_0995
- NMPDR:   fig|205918.4.peg.1314
- eggNOG:   COG1853
- HOGENOM:   HBG730363
- OMA:   NRSASVY
- ProtClustDB:   CLSK409983
- BioCyc:   PSYR205918:PSYR_0995-MONOMER
- HAMAP:   MF_00833
- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- Gene3D:   G3DSA:2.30.110.10
- SMART:   SM00903
- TIGRFAMs:   TIGR03615

Pfam domain/function: PF01613 Flavin_Reduct; SSF50475 FMN_binding

EC number: =1.5.1.29

Molecular weight: Translated: 18439; Mature: 18308

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSSATPAILPIAAVSQLAFRDAMSGLAAAVNLITTDGPGGRAGFTATAVCSVTDQPPT
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEECCCCCE
LLVCINRSASVYDAFIENGTLCVNTLGNGQQDLSNLFGGKSSQQERFAGGQWEAGVTGAP
EEEEECCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCC
VLDSAKLALDCKVSQSVSVGTHDILFCEVVDIRHQSGADALVYFGRRYHHLPSETPAA
CCCCCEEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCEEHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
PTSSATPAILPIAAVSQLAFRDAMSGLAAAVNLITTDGPGGRAGFTATAVCSVTDQPPT
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCEEEEEEEECCCCCE
LLVCINRSASVYDAFIENGTLCVNTLGNGQQDLSNLFGGKSSQQERFAGGQWEAGVTGAP
EEEEECCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCC
VLDSAKLALDCKVSQSVSVGTHDILFCEVVDIRHQSGADALVYFGRRYHHLPSETPAA
CCCCCEEEEEEECCCCCCCCCCEEEEEEEEEECCCCCCCCEEHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA