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The map label for this gene is hemK [H]

Identifier: 66044203

GI number: 66044203

Start: 1085984

End: 1086817

Strand: Direct

Name: hemK [H]

Synonym: Psyr_0950

Alternate gene names: 66044203

Gene position: 1085984-1086817 (Clockwise)

Preceding gene: 66044202

Following gene: 66044204

Centisome position: 17.82

GC content: 63.67

Gene sequence:

>834_bases
ATGACCATCATCGCAAGTGTACTCAGAAGCGCCGAGCTTCCCGATTCACCGACTGCACGGCTTGATGCCGAGCTTCTGCT
GGCGGCGGCGCTGGGCAAACCACGCAGTTTTCTGCACACCTGGCCCGAGCGCATCGTCAGCACCGAAGCCGCCGTGGCAT
TCGCCGGCTACATGGAGCGCCGCCGCAAGGGCGAGCCGGTCGCTTACATTCTCGGCCAGCAGGGCTTCTGGAAGCTGGAT
CTGGAAGTCGCCCCGCACACGTTGATCCCGCGCCCCGAAACCGAAATGCTGGTCGAGGCGGCACTGGAACTGGTGCCTGC
CTTCGCACCCGCTCAGGTGCTGGACCTCGGCACCGGCACCGGCGCAATTGCGCTGGCGCTGGCCAACGACCGCCAGCAAT
GGAAGGTTACTGCGGTTGACCGTGTGCCAGAGGCCGTCGCGCTGGCAGAGCGCAACCGGCAGCGCTTGCAACTGAACAAC
GCCGAAGTCTTCGAAAGCCACTGGTTCAGTGGCCTGCAAGGTCGACAGTTCGACCTGATCATCAGCAACCCGCCGTACAT
CTCTGACGCAGATCCGCATCTGTCGGCTGGCGACGTGCGTTTTGAGCCGAGCAGTGCGCTGGTGGCCGGTTCCGACGGGC
TGGATGACTTGCGCACGATCATCGAACAGGCGCCTGCACATCTCAATGCGGATGGCTGGCTGCTGCTGGAGCACGGCTAC
GATCAGGGACCTGCGGTGCGTGAGCTGCTGATTCGCCAGGGCTTCGAGCGTATCCAGACGCGTCGTGATCTGGGCGAGCA
TGAGCGCATCACGTTCGGATGCAAGCCGTGCTGA

Upstream 100 bases:

>100_bases
GTATTCGCTGGATGAAGTTCTGGCGGGCGGGGTCGATGCAGTGATAGAGCCGCTACTCGCTGAATATCAGGCTGATCAAC
TTGCGGCACTGGGTGAGTAA

Downstream 100 bases:

>100_bases
GTGATCAGGAACTGCTGCGCTACAGTCGCCAGATTCTGCTGCAACATGTTGATATCGATGGCCAGCTAAGACTCAAGCAA
AGTCGTGTGCTGGTCGTGGG

Product: modification methylase HemK

Products: NA

Alternate protein names: M.VchAHemK2P [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTIIASVLRSAELPDSPTARLDAELLLAAALGKPRSFLHTWPERIVSTEAAVAFAGYMERRRKGEPVAYILGQQGFWKLD
LEVAPHTLIPRPETEMLVEAALELVPAFAPAQVLDLGTGTGAIALALANDRQQWKVTAVDRVPEAVALAERNRQRLQLNN
AEVFESHWFSGLQGRQFDLIISNPPYISDADPHLSAGDVRFEPSSALVAGSDGLDDLRTIIEQAPAHLNADGWLLLEHGY
DQGPAVRELLIRQGFERIQTRRDLGEHERITFGCKPC

Sequences:

>Translated_277_residues
MTIIASVLRSAELPDSPTARLDAELLLAAALGKPRSFLHTWPERIVSTEAAVAFAGYMERRRKGEPVAYILGQQGFWKLD
LEVAPHTLIPRPETEMLVEAALELVPAFAPAQVLDLGTGTGAIALALANDRQQWKVTAVDRVPEAVALAERNRQRLQLNN
AEVFESHWFSGLQGRQFDLIISNPPYISDADPHLSAGDVRFEPSSALVAGSDGLDDLRTIIEQAPAHLNADGWLLLEHGY
DQGPAVRELLIRQGFERIQTRRDLGEHERITFGCKPC
>Mature_276_residues
TIIASVLRSAELPDSPTARLDAELLLAAALGKPRSFLHTWPERIVSTEAAVAFAGYMERRRKGEPVAYILGQQGFWKLDL
EVAPHTLIPRPETEMLVEAALELVPAFAPAQVLDLGTGTGAIALALANDRQQWKVTAVDRVPEAVALAERNRQRLQLNNA
EVFESHWFSGLQGRQFDLIISNPPYISDADPHLSAGDVRFEPSSALVAGSDGLDDLRTIIEQAPAHLNADGWLLLEHGYD
QGPAVRELLIRQGFERIQTRRDLGEHERITFGCKPC

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=240, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=2e-19,
Organism=Escherichia coli, GI1787463, Length=263, Percent_Identity=49.8098859315589, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI87082085, Length=197, Percent_Identity=32.994923857868, Blast_Score=97, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6324266, Length=162, Percent_Identity=32.0987654320988, Blast_Score=73, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24582226, Length=205, Percent_Identity=31.7073170731707, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30539; Mature: 30408

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIIASVLRSAELPDSPTARLDAELLLAAALGKPRSFLHTWPERIVSTEAAVAFAGYMER
CHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKGEPVAYILGQQGFWKLDLEVAPHTLIPRPETEMLVEAALELVPAFAPAQVLDLGTGT
HHCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCC
GAIALALANDRQQWKVTAVDRVPEAVALAERNRQRLQLNNAEVFESHWFSGLQGRQFDLI
CEEEEEEECCCCCEEEEEHHHCHHHHHHHHCCHHHEECCCHHHHHHHHHCCCCCCEEEEE
ISNPPYISDADPHLSAGDVRFEPSSALVAGSDGLDDLRTIIEQAPAHLNADGWLLLEHGY
ECCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCC
DQGPAVRELLIRQGFERIQTRRDLGEHERITFGCKPC
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
>Mature Secondary Structure 
TIIASVLRSAELPDSPTARLDAELLLAAALGKPRSFLHTWPERIVSTEAAVAFAGYMER
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RRKGEPVAYILGQQGFWKLDLEVAPHTLIPRPETEMLVEAALELVPAFAPAQVLDLGTGT
HHCCCCEEEEECCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHHEECCCCC
GAIALALANDRQQWKVTAVDRVPEAVALAERNRQRLQLNNAEVFESHWFSGLQGRQFDLI
CEEEEEEECCCCCEEEEEHHHCHHHHHHHHCCHHHEECCCHHHHHHHHHCCCCCCEEEEE
ISNPPYISDADPHLSAGDVRFEPSSALVAGSDGLDDLRTIIEQAPAHLNADGWLLLEHGY
ECCCCCCCCCCCCCCCCCEEECCCCEEEECCCCHHHHHHHHHHCCCCCCCCCEEEEECCC
DQGPAVRELLIRQGFERIQTRRDLGEHERITFGCKPC
CCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]