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The map label for this gene is actII-2 [H]

Identifier: 66044003

GI number: 66044003

Start: 849549

End: 850991

Strand: Reverse

Name: actII-2 [H]

Synonym: Psyr_0748

Alternate gene names: 66044003

Gene position: 850991-849549 (Counterclockwise)

Preceding gene: 66044004

Following gene: 66044002

Centisome position: 13.97

GC content: 54.12

Gene sequence:

>1443_bases
ATGTCAGAGGCTCCAACGCTAAAACCAAATCATGCGGAGGTTCTGACCGGCGCAAGGCGCTGGATGTGCTTGGCAATCCT
TCTTGTGGGGTCGTTCTTGCCGCCTCTAGATTACTTCATCATTAACATCGCCCTCCCTTTCATCAAGGCTGACTTGCGGG
CCAGCGATGGCATGATGCAGGCTTCGGTATCAATCTACGCGGCAGCCTTTGCACTATTCCTGATTCTCGGCGGGCGCTTG
GGAGACAGCTATGGTGCACGACGAACCTTTATCGTGGGCATGCTCGGCTTTGCATTTGCCTCGGCAGCCTGCGGTATGGC
CGGAAGCGATCTGGTCCTGGTTGTTGGCCGCTTCGTTCAGGGGTTGTTTGCCGCCATAATGGCCCCCCAATCCCTGGCAT
TAATCCACGCCAACTTCATTGGTAAAGACAAAGCACTTGCACTCTCGCTGTATGCTTCGATTTTTGGTCTAGCCTGTCTG
GTGGGGCAAGGCGCGGGAGGCCTGCTTATAGAAGCCAATATCGGCGGACTGAGCTGGCGCAGCTTGTTCCTAATAAATTT
GCCGGTTGTCTTGTTGTGCCTTTTAGCCGCAGTTCGCCTGCTCACCGAATCCACACTACAGCAGTCGAAATATATAGACA
TCCGAGGCTGCGCTTTATTCGCCGGATTCCTGCTACCGCTCCTAGCCTGCCTTATCGAGGCTGCGCCTAGAGGCTGGCCT
TGGTGGTCGTGGCTTGCACTTACCCTAAGCGCCTTCTGCCTCAAGCTATTTGTTACTTGGGAAAACCAACTCAAACGTGG
AGGAAGAGCACCCTTTGTCGATCTGGCGATCTTTAACTTACCGCCGTTGTTACCCGGCGTTCTAGCGCTGTTCTGCTTCT
ATGCCATTTCGCCATTCTTTCTGATTTATGCCGACTTCCTACAGTCGGGTTTCCAACTTAGTCCGGCGGCGGTAGGCGCT
AGGATCCTGCCTTTCGGTATTGGCTTTTTCATTGCAGCATTGAGCAGTGCTTTTCTTGGTCAGCGCCAGGGTAAGCGCGG
GGCAATGTTGGGTTTGAGCCTTCAGGCGGCGAGCATGCTTAGTGTGGTAATGTGCATTTGCGGCGGGCGCCCGGATCTGC
TTTACCTGCCCTTGATCTTGCTTGGCGCAGGACAAGGTTTCGCATTGCCGGCCATGGTAGGGGTAGTCAGTGACACCCTT
AATCGCCATCATCCAGGCATGAGTTCCGGGCTTATCAATACCGTCCTACAATGCAGTTCAGCGTTTTTTGTGGCCAGCGT
TGGCGGGGTGTTCTTCGGAGTTAAAAACGCTATTCCCGGCATAATCGGGCTGTCCGATGCATTGGTGGCCGCCACTTCGC
TGATTCTGGTATGTCAACTGCTGGCACTTTTGTTAATCCGTATCTCAATCTCACGAGAAGCCGCCAGCACCGCCGAACAC
TAG

Upstream 100 bases:

>100_bases
TCCAGTACCTCGACTCGGGACATCTGAGCATGCTCACCCACCCAAGCCTACTGGCTCAAACCCTAATGGAACTGGGTTCC
AGATATCGGGAGGTTGACCA

Downstream 100 bases:

>100_bases
TCAAACCGCTAAATAATAAAACCAGGCAAAGGCGAAAACGAGTCCAGCATGACTGGCTCTCTAACGGACAGCGACTGACT
CCTTTTGTGGGGCTTAGAGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSEAPTLKPNHAEVLTGARRWMCLAILLVGSFLPPLDYFIINIALPFIKADLRASDGMMQASVSIYAAAFALFLILGGRL
GDSYGARRTFIVGMLGFAFASAACGMAGSDLVLVVGRFVQGLFAAIMAPQSLALIHANFIGKDKALALSLYASIFGLACL
VGQGAGGLLIEANIGGLSWRSLFLINLPVVLLCLLAAVRLLTESTLQQSKYIDIRGCALFAGFLLPLLACLIEAAPRGWP
WWSWLALTLSAFCLKLFVTWENQLKRGGRAPFVDLAIFNLPPLLPGVLALFCFYAISPFFLIYADFLQSGFQLSPAAVGA
RILPFGIGFFIAALSSAFLGQRQGKRGAMLGLSLQAASMLSVVMCICGGRPDLLYLPLILLGAGQGFALPAMVGVVSDTL
NRHHPGMSSGLINTVLQCSSAFFVASVGGVFFGVKNAIPGIIGLSDALVAATSLILVCQLLALLLIRISISREAASTAEH

Sequences:

>Translated_480_residues
MSEAPTLKPNHAEVLTGARRWMCLAILLVGSFLPPLDYFIINIALPFIKADLRASDGMMQASVSIYAAAFALFLILGGRL
GDSYGARRTFIVGMLGFAFASAACGMAGSDLVLVVGRFVQGLFAAIMAPQSLALIHANFIGKDKALALSLYASIFGLACL
VGQGAGGLLIEANIGGLSWRSLFLINLPVVLLCLLAAVRLLTESTLQQSKYIDIRGCALFAGFLLPLLACLIEAAPRGWP
WWSWLALTLSAFCLKLFVTWENQLKRGGRAPFVDLAIFNLPPLLPGVLALFCFYAISPFFLIYADFLQSGFQLSPAAVGA
RILPFGIGFFIAALSSAFLGQRQGKRGAMLGLSLQAASMLSVVMCICGGRPDLLYLPLILLGAGQGFALPAMVGVVSDTL
NRHHPGMSSGLINTVLQCSSAFFVASVGGVFFGVKNAIPGIIGLSDALVAATSLILVCQLLALLLIRISISREAASTAEH
>Mature_479_residues
SEAPTLKPNHAEVLTGARRWMCLAILLVGSFLPPLDYFIINIALPFIKADLRASDGMMQASVSIYAAAFALFLILGGRLG
DSYGARRTFIVGMLGFAFASAACGMAGSDLVLVVGRFVQGLFAAIMAPQSLALIHANFIGKDKALALSLYASIFGLACLV
GQGAGGLLIEANIGGLSWRSLFLINLPVVLLCLLAAVRLLTESTLQQSKYIDIRGCALFAGFLLPLLACLIEAAPRGWPW
WSWLALTLSAFCLKLFVTWENQLKRGGRAPFVDLAIFNLPPLLPGVLALFCFYAISPFFLIYADFLQSGFQLSPAAVGAR
ILPFGIGFFIAALSSAFLGQRQGKRGAMLGLSLQAASMLSVVMCICGGRPDLLYLPLILLGAGQGFALPAMVGVVSDTLN
RHHPGMSSGLINTVLQCSSAFFVASVGGVFFGVKNAIPGIIGLSDALVAATSLILVCQLLALLLIRISISREAASTAEH

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=405, Percent_Identity=27.4074074074074, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI87081983, Length=430, Percent_Identity=25.8139534883721, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788710, Length=283, Percent_Identity=26.5017667844523, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789042, Length=211, Percent_Identity=27.9620853080569, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788392, Length=207, Percent_Identity=28.9855072463768, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50754; Mature: 50623

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAPTLKPNHAEVLTGARRWMCLAILLVGSFLPPLDYFIINIALPFIKADLRASDGMMQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
ASVSIYAAAFALFLILGGRLGDSYGARRTFIVGMLGFAFASAACGMAGSDLVLVVGRFVQ
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GLFAAIMAPQSLALIHANFIGKDKALALSLYASIFGLACLVGQGAGGLLIEANIGGLSWR
HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHH
SLFLINLPVVLLCLLAAVRLLTESTLQQSKYIDIRGCALFAGFLLPLLACLIEAAPRGWP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
WWSWLALTLSAFCLKLFVTWENQLKRGGRAPFVDLAIFNLPPLLPGVLALFCFYAISPFF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHH
LIYADFLQSGFQLSPAAVGARILPFGIGFFIAALSSAFLGQRQGKRGAMLGLSLQAASML
HHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHH
SVVMCICGGRPDLLYLPLILLGAGQGFALPAMVGVVSDTLNRHHPGMSSGLINTVLQCSS
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AFFVASVGGVFFGVKNAIPGIIGLSDALVAATSLILVCQLLALLLIRISISREAASTAEH
HHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEAPTLKPNHAEVLTGARRWMCLAILLVGSFLPPLDYFIINIALPFIKADLRASDGMMQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHH
ASVSIYAAAFALFLILGGRLGDSYGARRTFIVGMLGFAFASAACGMAGSDLVLVVGRFVQ
HHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
GLFAAIMAPQSLALIHANFIGKDKALALSLYASIFGLACLVGQGAGGLLIEANIGGLSWR
HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHH
SLFLINLPVVLLCLLAAVRLLTESTLQQSKYIDIRGCALFAGFLLPLLACLIEAAPRGWP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
WWSWLALTLSAFCLKLFVTWENQLKRGGRAPFVDLAIFNLPPLLPGVLALFCFYAISPFF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHHH
LIYADFLQSGFQLSPAAVGARILPFGIGFFIAALSSAFLGQRQGKRGAMLGLSLQAASML
HHHHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHH
SVVMCICGGRPDLLYLPLILLGAGQGFALPAMVGVVSDTLNRHHPGMSSGLINTVLQCSS
HHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AFFVASVGGVFFGVKNAIPGIIGLSDALVAATSLILVCQLLALLLIRISISREAASTAEH
HHHHHHHHHHHHHHHHHCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]