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The map label for this gene is ispB [H]

Identifier: 66043965

GI number: 66043965

Start: 803577

End: 804545

Strand: Reverse

Name: ispB [H]

Synonym: Psyr_0700

Alternate gene names: 66043965

Gene position: 804545-803577 (Counterclockwise)

Preceding gene: 66043972

Following gene: 66043962

Centisome position: 13.2

GC content: 62.13

Gene sequence:

>969_bases
ATGCAACCCCAAGCCTTCTACCGCGCGGTGGCGGACGACTTTAGTGCCGTTGACCTTATCATCAAAAAGCAACTGACGTC
GCGCGTACCGCTGGTCTCGAAAATCGGCGATTACATCACGTCGGCAGGTGGCAAGCGCCTGCGCCCGTTGCTGGTGCTGC
TGTGTGGCAAGGCACTGGGTCGCGAAGGCGATGATGTTCGCCTGCTCGCAGCCACCATTGAATTCCTGCACACCGCGACC
CTGCTGCATGACGACGTGGTCGACATGTCCGGCATGCGTCGTGGTCGCTCCACCGCCAACGCGCTCTGGGGCAATGCGCC
CAGCGTATTGGTGGGCGACTTCCTTTATTCGCGCTCGTTCGAAATGATGGTCGAGCTGGGCTCGATGGAAGTCATGAAGA
TTCTGTCCAAGGCAACCCGCATCATCGCCGAAGGCGAAGTGTTGCAGCTCTCGAAGATTCGTGACGCCAGCACCACCGAA
GAAACCTACATGGAAGTGATTCGTGGCAAGACAGCCATGCTGTTCGAAGCCTCGACCCACAGCGCCGCTGCCCTGTGCAA
CGCCCCCCAGGAACAGAGCGAAGCACTGCGCACGTTCGGCGACCACCTGGGTGTGGCCTTCCAGCTGGTGGATGACCTGC
TCGACTACCGTGGCGATGCCGAGACACTGGGCAAGAACGTCGGTGACGACCTCGCCGAAGGCAAGCCGACCCTGCCGCTG
ATCTACACCATGCGCGAAGGCAGCCCGGAACAGGCCGCACTGGTTCGCCAGGCGATCCAGAAAGGTGGTCTGGAAGATCT
GGAAAGCATCCGCAATGCAGTGGAAAGCGCCGGTGCCCTCGATTACACCGCACAGCTGGCCCGCGACTACGCGGCCCGCG
CCATTGCCTGCCTGGACGCGCTGCCGCCCAGCGAGTACCGCGACGCACTGGTCGAACTGAGCGAGTTTGCGGTCGCCCGC
ACGCACTGA

Upstream 100 bases:

>100_bases
CAAGGCATTCGCGTGTTGCAAGAGGCGGCTCGTCATACCTTGACACGCTCTACCCCGCGACCTAGCATGCGGCGCAACCT
ATCCGGAGCACCTGTCGCTG

Downstream 100 bases:

>100_bases
CATTCGCAGCAGTCAAAAGCCCGTCCAGCGACGGGCTCGGCAAATGGCAGTCAGCGCGTAAGCCTTCATCTTCTCCTGCA
TGAAACCCGGAAAACACTGA

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MQPQAFYRAVADDFSAVDLIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALGREGDDVRLLAATIEFLHTAT
LLHDDVVDMSGMRRGRSTANALWGNAPSVLVGDFLYSRSFEMMVELGSMEVMKILSKATRIIAEGEVLQLSKIRDASTTE
ETYMEVIRGKTAMLFEASTHSAAALCNAPQEQSEALRTFGDHLGVAFQLVDDLLDYRGDAETLGKNVGDDLAEGKPTLPL
IYTMREGSPEQAALVRQAIQKGGLEDLESIRNAVESAGALDYTAQLARDYAARAIACLDALPPSEYRDALVELSEFAVAR
TH

Sequences:

>Translated_322_residues
MQPQAFYRAVADDFSAVDLIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALGREGDDVRLLAATIEFLHTAT
LLHDDVVDMSGMRRGRSTANALWGNAPSVLVGDFLYSRSFEMMVELGSMEVMKILSKATRIIAEGEVLQLSKIRDASTTE
ETYMEVIRGKTAMLFEASTHSAAALCNAPQEQSEALRTFGDHLGVAFQLVDDLLDYRGDAETLGKNVGDDLAEGKPTLPL
IYTMREGSPEQAALVRQAIQKGGLEDLESIRNAVESAGALDYTAQLARDYAARAIACLDALPPSEYRDALVELSEFAVAR
TH
>Mature_322_residues
MQPQAFYRAVADDFSAVDLIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALGREGDDVRLLAATIEFLHTAT
LLHDDVVDMSGMRRGRSTANALWGNAPSVLVGDFLYSRSFEMMVELGSMEVMKILSKATRIIAEGEVLQLSKIRDASTTE
ETYMEVIRGKTAMLFEASTHSAAALCNAPQEQSEALRTFGDHLGVAFQLVDDLLDYRGDAETLGKNVGDDLAEGKPTLPL
IYTMREGSPEQAALVRQAIQKGGLEDLESIRNAVESAGALDYTAQLARDYAARAIACLDALPPSEYRDALVELSEFAVAR
TH

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=308, Percent_Identity=31.1688311688312, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI4758430, Length=268, Percent_Identity=26.865671641791, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI83700220, Length=268, Percent_Identity=26.865671641791, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI169808399, Length=333, Percent_Identity=21.6216216216216, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1789578, Length=321, Percent_Identity=51.0903426791277, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1786623, Length=271, Percent_Identity=28.4132841328413, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505681, Length=334, Percent_Identity=31.437125748503, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=31.7725752508361, Blast_Score=125, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325188, Length=233, Percent_Identity=24.0343347639485, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24651612, Length=299, Percent_Identity=29.7658862876254, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281365769, Length=279, Percent_Identity=25.089605734767, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660002, Length=279, Percent_Identity=25.089605734767, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35027; Mature: 35027

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPQAFYRAVADDFSAVDLIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
REGDDVRLLAATIEFLHTATLLHDDVVDMSGMRRGRSTANALWGNAPSVLVGDFLYSRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHH
EMMVELGSMEVMKILSKATRIIAEGEVLQLSKIRDASTTEETYMEVIRGKTAMLFEASTH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCC
SAAALCNAPQEQSEALRTFGDHLGVAFQLVDDLLDYRGDAETLGKNVGDDLAEGKPTLPL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCEE
IYTMREGSPEQAALVRQAIQKGGLEDLESIRNAVESAGALDYTAQLARDYAARAIACLDA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
LPPSEYRDALVELSEFAVARTH
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQPQAFYRAVADDFSAVDLIIKKQLTSRVPLVSKIGDYITSAGGKRLRPLLVLLCGKALG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
REGDDVRLLAATIEFLHTATLLHDDVVDMSGMRRGRSTANALWGNAPSVLVGDFLYSRSF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHH
EMMVELGSMEVMKILSKATRIIAEGEVLQLSKIRDASTTEETYMEVIRGKTAMLFEASTH
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHCCCCEEEEECCCC
SAAALCNAPQEQSEALRTFGDHLGVAFQLVDDLLDYRGDAETLGKNVGDDLAEGKPTLPL
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCCCCCEE
IYTMREGSPEQAALVRQAIQKGGLEDLESIRNAVESAGALDYTAQLARDYAARAIACLDA
EEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
LPPSEYRDALVELSEFAVARTH
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]