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The map label for this gene is metC [H]

Identifier: 66043874

GI number: 66043874

Start: 696911

End: 698077

Strand: Reverse

Name: metC [H]

Synonym: Psyr_0607

Alternate gene names: 66043874

Gene position: 698077-696911 (Counterclockwise)

Preceding gene: 66043875

Following gene: 66043873

Centisome position: 11.46

GC content: 62.9

Gene sequence:

>1167_bases
ATGCCGCAAGACAAAGACCCTCGTGCCCACACCGCCCTGTCGCAACGCGGCCGCAGCGCCGGCAGCGGGCCCGGGATTGC
GGTCAACCCACCCGTCGTGCGCCTCAGTACGGTGCTGTTCGACAGTCTGGAAAGCTTGCGGCAGGCGGAAATTCGCACCA
CGGGGCCGGAGCGCTCGTTGACTTACGGCGCCAACGGCAACCCGACTGCGTTTGCCTTGCAGGACCTGATCTCCGAGCTC
GAAGGCGCACACGGCACCTGCCTGTACCCGACCGGGCTGGCCGCAGTGGCACAGATGTTTCAATCGTTTCTGCGCCCAGG
CGATCATGTCCTGATCACCGAATCGGTCTATGGCCCTGTTCGACGGCTGGCGAAAACCATGCTGACCGCCTTCGACATCC
AGTTCGACTTCTACGCGGCCGACGGCAGTAACGTGGAATCGCTGATCAAGGCCAACACCCGGATGATCTATGCCGAGGTG
CCCGGCTCGCTGACATTCGACATGTGTGACCTGCCCGCCCTGTCACAGCTGTGCAAAGCAAGGAATCTGCTGCTGGCTGT
GGATAACTCCTGGGGCTCGGGGGTGCTGTACAAGCCGCTGGCGCTGGGCGCGGACATTTCGCTGATGGCCCTGACCAAGT
ACGTGGCCGGCCATTCCGACGTCATGATGGGCAGCGTCAGCACCACCGAAGCGCATTGGCATACCCTGAAAACCATGAAC
ACCGCCGTGGGCAATACCGTCAGCCCCGACGATGCCTATCTGGTGCTGCGTGGTGCCCGCAGCCTGGCGGCGCGCATGGC
CATGCATGAGCGCCATGCGCTGCAGATCGCACAATGGCTGCAGGCCCAGCCTCAGGTTGCCCGCGTGCTCTATCCCGCTT
TGCCGGACGATCCAGGCCACGCGATCTGGAAGCGCGATTTCCATGGCTGCAACGGCTTGCTCAGTTTCGAATTCAACACC
GCTGACCGGCAGGTGCTGGATCGCTTTACCGGCGCGTTGAAACTGTTCGGCATCGGCTATTCCTGGGGTGGTTTTGAAAG
CCTGATCACGGAAGTGGAACAACACGGTCCCGAGCGCGGTGCAGGGCCGATGCTGCGGCTGCAGGTCGGCCTGGAAAGCC
CGGAAGACCTGATCGCAGACCTGCGCAACGGATTCGCGGCAGAGTGA

Upstream 100 bases:

>100_bases
GCTGTGTTGAACGATCAGTGATACCTGCCAGGCGCACGATGCATTACGCTTGCGGGTACCTGAGCCGCAGCCTGCAAGCC
TTTTCCACCCGGAGCCTCCA

Downstream 100 bases:

>100_bases
CCCCGGCAGCCGCTTTAAGAGCGCGGCTCCGTCGCCGCAATTTGCCCAGACGCCATCGCTCTGCTAGTGTCGCGCGGTTT
AACCTCTACCGGAAATGCCC

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase; Osteotoxin [H]

Number of amino acids: Translated: 388; Mature: 387

Protein sequence:

>388_residues
MPQDKDPRAHTALSQRGRSAGSGPGIAVNPPVVRLSTVLFDSLESLRQAEIRTTGPERSLTYGANGNPTAFALQDLISEL
EGAHGTCLYPTGLAAVAQMFQSFLRPGDHVLITESVYGPVRRLAKTMLTAFDIQFDFYAADGSNVESLIKANTRMIYAEV
PGSLTFDMCDLPALSQLCKARNLLLAVDNSWGSGVLYKPLALGADISLMALTKYVAGHSDVMMGSVSTTEAHWHTLKTMN
TAVGNTVSPDDAYLVLRGARSLAARMAMHERHALQIAQWLQAQPQVARVLYPALPDDPGHAIWKRDFHGCNGLLSFEFNT
ADRQVLDRFTGALKLFGIGYSWGGFESLITEVEQHGPERGAGPMLRLQVGLESPEDLIADLRNGFAAE

Sequences:

>Translated_388_residues
MPQDKDPRAHTALSQRGRSAGSGPGIAVNPPVVRLSTVLFDSLESLRQAEIRTTGPERSLTYGANGNPTAFALQDLISEL
EGAHGTCLYPTGLAAVAQMFQSFLRPGDHVLITESVYGPVRRLAKTMLTAFDIQFDFYAADGSNVESLIKANTRMIYAEV
PGSLTFDMCDLPALSQLCKARNLLLAVDNSWGSGVLYKPLALGADISLMALTKYVAGHSDVMMGSVSTTEAHWHTLKTMN
TAVGNTVSPDDAYLVLRGARSLAARMAMHERHALQIAQWLQAQPQVARVLYPALPDDPGHAIWKRDFHGCNGLLSFEFNT
ADRQVLDRFTGALKLFGIGYSWGGFESLITEVEQHGPERGAGPMLRLQVGLESPEDLIADLRNGFAAE
>Mature_387_residues
PQDKDPRAHTALSQRGRSAGSGPGIAVNPPVVRLSTVLFDSLESLRQAEIRTTGPERSLTYGANGNPTAFALQDLISELE
GAHGTCLYPTGLAAVAQMFQSFLRPGDHVLITESVYGPVRRLAKTMLTAFDIQFDFYAADGSNVESLIKANTRMIYAEVP
GSLTFDMCDLPALSQLCKARNLLLAVDNSWGSGVLYKPLALGADISLMALTKYVAGHSDVMMGSVSTTEAHWHTLKTMNT
AVGNTVSPDDAYLVLRGARSLAARMAMHERHALQIAQWLQAQPQVARVLYPALPDDPGHAIWKRDFHGCNGLLSFEFNTA
DRQVLDRFTGALKLFGIGYSWGGFESLITEVEQHGPERGAGPMLRLQVGLESPEDLIADLRNGFAAE

Specific function: Also has cytotoxic activity toward osteogenic, osteosarcoma and tracheal cells, in vitro. The chemical basis for cell toxicity might be the formation and subsequent transfer of sulfane-sulfur to proteins, derived via beta-cystathionase cleavage of L-cysti

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=299, Percent_Identity=34.7826086956522, Blast_Score=183, Evalue=2e-46,
Organism=Homo sapiens, GI299473759, Length=299, Percent_Identity=32.1070234113712, Blast_Score=151, Evalue=9e-37,
Organism=Homo sapiens, GI25453487, Length=295, Percent_Identity=32.2033898305085, Blast_Score=147, Evalue=1e-35,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=37.4045801526718, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1790375, Length=347, Percent_Identity=35.4466858789625, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=376, Percent_Identity=31.3829787234043, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17538055, Length=299, Percent_Identity=35.4515050167224, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17560056, Length=299, Percent_Identity=35.1170568561873, Blast_Score=180, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6321170, Length=336, Percent_Identity=36.6071428571429, Blast_Score=222, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6319307, Length=349, Percent_Identity=29.512893982808, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323333, Length=379, Percent_Identity=24.0105540897098, Blast_Score=91, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6321254, Length=363, Percent_Identity=24.5179063360882, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321904, Length=360, Percent_Identity=25.2777777777778, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922554, Length=383, Percent_Identity=31.8537859007833, Blast_Score=191, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 42071; Mature: 41940

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQDKDPRAHTALSQRGRSAGSGPGIAVNPPVVRLSTVLFDSLESLRQAEIRTTGPERSL
CCCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TYGANGNPTAFALQDLISELEGAHGTCLYPTGLAAVAQMFQSFLRPGDHVLITESVYGPV
EECCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
RRLAKTMLTAFDIQFDFYAADGSNVESLIKANTRMIYAEVPGSLTFDMCDLPALSQLCKA
HHHHHHHHHHHEEEEEEEECCCCCHHHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHH
RNLLLAVDNSWGSGVLYKPLALGADISLMALTKYVAGHSDVMMGSVSTTEAHWHTLKTMN
CCEEEEEECCCCCCEEECHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
TAVGNTVSPDDAYLVLRGARSLAARMAMHERHALQIAQWLQAQPQVARVLYPALPDDPGH
HHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
AIWKRDFHGCNGLLSFEFNTADRQVLDRFTGALKLFGIGYSWGGFESLITEVEQHGPERG
HHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCCCC
AGPMLRLQVGLESPEDLIADLRNGFAAE
CCCEEEEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PQDKDPRAHTALSQRGRSAGSGPGIAVNPPVVRLSTVLFDSLESLRQAEIRTTGPERSL
CCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
TYGANGNPTAFALQDLISELEGAHGTCLYPTGLAAVAQMFQSFLRPGDHVLITESVYGPV
EECCCCCCHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHH
RRLAKTMLTAFDIQFDFYAADGSNVESLIKANTRMIYAEVPGSLTFDMCDLPALSQLCKA
HHHHHHHHHHHEEEEEEEECCCCCHHHHHHCCCEEEEEECCCCEEECCCCCHHHHHHHHH
RNLLLAVDNSWGSGVLYKPLALGADISLMALTKYVAGHSDVMMGSVSTTEAHWHTLKTMN
CCEEEEEECCCCCCEEECHHHCCCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
TAVGNTVSPDDAYLVLRGARSLAARMAMHERHALQIAQWLQAQPQVARVLYPALPDDPGH
HHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCC
AIWKRDFHGCNGLLSFEFNTADRQVLDRFTGALKLFGIGYSWGGFESLITEVEQHGPERG
HHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHCCCCCC
AGPMLRLQVGLESPEDLIADLRNGFAAE
CCCEEEEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8463265 [H]