Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 66043863

GI number: 66043863

Start: 684299

End: 685204

Strand: Reverse

Name: gcvA [H]

Synonym: Psyr_0596

Alternate gene names: 66043863

Gene position: 685204-684299 (Counterclockwise)

Preceding gene: 66043870

Following gene: 66043862

Centisome position: 11.24

GC content: 60.71

Gene sequence:

>906_bases
ATGCTGAACAAACGTCATCTGCCCTCGATAGCCGCATTGCAATGCTTTGAGGCAGTGACCCGGCACCTGAGCTTCACGCG
CGCAGCAGAAGAACTCAACCTGACCCAGAGCGCCGTCAGCAAGCAGGTCGCGCAACTCGAAGAGCTGGTTCAGCACCTGC
TGTTTCAGCGCGTGCGCCGCCGTCTGCAACTGACGCCAGCCGGGGCGCTGTACCTGGCTGAAGTGCGCAAGATCCTGACC
CAGATCGAGATGTCTACCCACTTCCTGCGCTCCTACGGCGGAGAAACCGAGGTGTTGCGCGTCTCCACCCCGTCGACATT
TGGCGCGCGCTGGCTGGTGCCGCGCCTCAAGGGCTGGCGCTTGCGTCACCCGCACATTCACCTGGACCTGATCAACGAAC
AGGAATCCGATGACCTGATCAAGGGCCGCTGTGACGTGTCGTTCTATTTCGGCCAGGCGGCCATGCCCGGCGCAGAAAGC
GTGAAGCTGTTCGGTGAGGAACTGGTTCCGGTGTGCGCCCCCGAAAGCCTGCCGGACGAGCCTCTCGCCGACCCGACTCA
ACTGAGTGAACTGGTCCTGTTGCAGAATGCCTTCCGTCCCGAAGCCTGGCATGAATGGTTCGAAAGCCAGGGCTATCACA
CCGACCACAGCTATCACGGGCCGCGCTTTGAAACCTTCTATATGTGCATCCGCGCGGCACAGGTCGGCTGCGGTGTTGCG
CTGCTGCCACGCTTTCTGGTCGAGGAGGAACTGGCCGAGGGCAAGCTGGTGATTGCCTGGGATTACGCATTGCCCAGCAC
TAACAGCGCCTACTACCTGTCCTATCCGGAACACACCGCCGACGTGCCCAAAATCCGGGTTTTTCTACAGTGGATGCTGG
AACAGTTGGATCAACCCCCCGCTTAA

Upstream 100 bases:

>100_bases
TCCCCGCATACACCCGACAAACGACCTTTAAGCGAGATATCATTCCGATTATTCATAACGCTTACGCCAGCCCCCGCGCG
CCGCAGACAGAGAAGTCATC

Downstream 100 bases:

>100_bases
GGGCGACAATGCACACCCATCCGGCCGTGAGCCTTCCATGGTGACAGACAGGAAGTCACGCGCCCCACTTTATCCGGCTG
CTCTGGAGAGCCTGATTCAA

Product: regulatory protein, LysR:LysR, substrate-binding

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLNKRHLPSIAALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELVQHLLFQRVRRRLQLTPAGALYLAEVRKILT
QIEMSTHFLRSYGGETEVLRVSTPSTFGARWLVPRLKGWRLRHPHIHLDLINEQESDDLIKGRCDVSFYFGQAAMPGAES
VKLFGEELVPVCAPESLPDEPLADPTQLSELVLLQNAFRPEAWHEWFESQGYHTDHSYHGPRFETFYMCIRAAQVGCGVA
LLPRFLVEEELAEGKLVIAWDYALPSTNSAYYLSYPEHTADVPKIRVFLQWMLEQLDQPPA

Sequences:

>Translated_301_residues
MLNKRHLPSIAALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELVQHLLFQRVRRRLQLTPAGALYLAEVRKILT
QIEMSTHFLRSYGGETEVLRVSTPSTFGARWLVPRLKGWRLRHPHIHLDLINEQESDDLIKGRCDVSFYFGQAAMPGAES
VKLFGEELVPVCAPESLPDEPLADPTQLSELVLLQNAFRPEAWHEWFESQGYHTDHSYHGPRFETFYMCIRAAQVGCGVA
LLPRFLVEEELAEGKLVIAWDYALPSTNSAYYLSYPEHTADVPKIRVFLQWMLEQLDQPPA
>Mature_301_residues
MLNKRHLPSIAALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELVQHLLFQRVRRRLQLTPAGALYLAEVRKILT
QIEMSTHFLRSYGGETEVLRVSTPSTFGARWLVPRLKGWRLRHPHIHLDLINEQESDDLIKGRCDVSFYFGQAAMPGAES
VKLFGEELVPVCAPESLPDEPLADPTQLSELVLLQNAFRPEAWHEWFESQGYHTDHSYHGPRFETFYMCIRAAQVGCGVA
LLPRFLVEEELAEGKLVIAWDYALPSTNSAYYLSYPEHTADVPKIRVFLQWMLEQLDQPPA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=35.958904109589, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=32.1917808219178, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=28.9115646258503, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=24.496644295302, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693193, Length=302, Percent_Identity=25.1655629139073, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789639, Length=255, Percent_Identity=27.843137254902, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI157672245, Length=210, Percent_Identity=30.4761904761905, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=27.6666666666667, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788887, Length=181, Percent_Identity=32.0441988950276, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787589, Length=286, Percent_Identity=27.6223776223776, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790783, Length=261, Percent_Identity=24.904214559387, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787806, Length=257, Percent_Identity=25.2918287937743, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34367; Mature: 34367

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNKRHLPSIAALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELVQHLLFQRVRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQLTPAGALYLAEVRKILTQIEMSTHFLRSYGGETEVLRVSTPSTFGARWLVPRLKGWR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCE
LRHPHIHLDLINEQESDDLIKGRCDVSFYFGQAAMPGAESVKLFGEELVPVCAPESLPDE
ECCCEEEEEECCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
PLADPTQLSELVLLQNAFRPEAWHEWFESQGYHTDHSYHGPRFETFYMCIRAAQVGCGVA
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
LLPRFLVEEELAEGKLVIAWDYALPSTNSAYYLSYPEHTADVPKIRVFLQWMLEQLDQPP
HHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
A
C
>Mature Secondary Structure
MLNKRHLPSIAALQCFEAVTRHLSFTRAAEELNLTQSAVSKQVAQLEELVQHLLFQRVRR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLQLTPAGALYLAEVRKILTQIEMSTHFLRSYGGETEVLRVSTPSTFGARWLVPRLKGWR
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCCE
LRHPHIHLDLINEQESDDLIKGRCDVSFYFGQAAMPGAESVKLFGEELVPVCAPESLPDE
ECCCEEEEEECCCCCCCCHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
PLADPTQLSELVLLQNAFRPEAWHEWFESQGYHTDHSYHGPRFETFYMCIRAAQVGCGVA
CCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
LLPRFLVEEELAEGKLVIAWDYALPSTNSAYYLSYPEHTADVPKIRVFLQWMLEQLDQPP
HHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]