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The map label for this gene is nadE [H]

Identifier: 66043861

GI number: 66043861

Start: 682122

End: 682949

Strand: Reverse

Name: nadE [H]

Synonym: Psyr_0594

Alternate gene names: 66043861

Gene position: 682949-682122 (Counterclockwise)

Preceding gene: 66043862

Following gene: 66043860

Centisome position: 11.21

GC content: 64.01

Gene sequence:

>828_bases
ATGCACGCCGTACAGCGCCAGATTGCTGAACAGCTCAAGGTCCAGCCACCTTTCGCGGACCAGAACGCGCTGCAGGCCGA
GGTCGCCCGCCGCGTCAACTTCATCAAGGAGTGCCTGCAGAACGCACGGCTCAAGACTCTGGTGCTCGGCATCAGCGGCG
GAGTCGACTCGCTGACGGCCGGCCTGCTCGCCCAACGCGCGGTCAAGGAACTGCGTGAAAGCACGGGTGACAACGCCTAT
CGCTTCATCGCCGTGCGCCTGCCTTATGTGGTGCAGGCCGATGAGCACGAAGCCCAGGCCTCTGTCGACTTCATCGAGCC
GGACGAGCGCCATACCATCAACATCGGCAGCAGCGTCAAGGCCCTGGCCGCTGAGGTGAAGGCCTTCGACGGGCTTCCAG
CGACTTCGGTGGATTTCGTGCTCGGCAACACCAAGGCGCGCATGCGCATGGTCGCGCAGTACACCGTCGCCGGCGCTTAC
CATGGCCTTGTGATCGGCACCGATCACGCGGCCGAAGCGGTGATGGGCTTCTTCACCAAGTTCGGCGACGGCGCATGCGA
CCTGGCACCGTTGAGCGGCCTGGTGAAAAACCAGGTACGCGCCATCGCCCGCCATTTCGGCGCACCTGAATCACTGGTCG
AGAAAGTGCCGACTGCGGACCTGGAAGACCTGTCACCCGGCAAACCGGACGAAGCTTCGCACGGCGTGACCTACGCCGAG
ATCGACGCCTTCCTGCACGGCGAGCCGGTACGCGAGGAAGCGTTCCGGATCATTTGCGAAACCTACGCCAAGACCCAGCA
CAAGCGCGAGCTGCCTTACGCGCCGTGA

Upstream 100 bases:

>100_bases
ACCCGAACTTTGTCGCTTACCTGCGGCATGTGTTCAAGGTGCCGGATTTGCCGAGCCCGGAAAAACCCGCTTGAATTGTC
CTATCTACGAGGAGTGAATC

Downstream 100 bases:

>100_bases
GTGAGTGCGGAACGAGAGCGCTGCAGCACTATCGTGCCCATGCTCCGCGTGGGTACGCCTTTCTGGACGCTCTGCGTCCG
CTTCTGAATGCGCGGCGCGG

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MHAVQRQIAEQLKVQPPFADQNALQAEVARRVNFIKECLQNARLKTLVLGISGGVDSLTAGLLAQRAVKELRESTGDNAY
RFIAVRLPYVVQADEHEAQASVDFIEPDERHTINIGSSVKALAAEVKAFDGLPATSVDFVLGNTKARMRMVAQYTVAGAY
HGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARHFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
IDAFLHGEPVREEAFRIICETYAKTQHKRELPYAP

Sequences:

>Translated_275_residues
MHAVQRQIAEQLKVQPPFADQNALQAEVARRVNFIKECLQNARLKTLVLGISGGVDSLTAGLLAQRAVKELRESTGDNAY
RFIAVRLPYVVQADEHEAQASVDFIEPDERHTINIGSSVKALAAEVKAFDGLPATSVDFVLGNTKARMRMVAQYTVAGAY
HGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARHFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
IDAFLHGEPVREEAFRIICETYAKTQHKRELPYAP
>Mature_275_residues
MHAVQRQIAEQLKVQPPFADQNALQAEVARRVNFIKECLQNARLKTLVLGISGGVDSLTAGLLAQRAVKELRESTGDNAY
RFIAVRLPYVVQADEHEAQASVDFIEPDERHTINIGSSVKALAAEVKAFDGLPATSVDFVLGNTKARMRMVAQYTVAGAY
HGLVIGTDHAAEAVMGFFTKFGDGACDLAPLSGLVKNQVRAIARHFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
IDAFLHGEPVREEAFRIICETYAKTQHKRELPYAP

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=270, Percent_Identity=48.8888888888889, Blast_Score=234, Evalue=6e-63,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 29892; Mature: 29892

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAVQRQIAEQLKVQPPFADQNALQAEVARRVNFIKECLQNARLKTLVLGISGGVDSLTA
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHH
GLLAQRAVKELRESTGDNAYRFIAVRLPYVVQADEHEAQASVDFIEPDERHTINIGSSVK
HHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCHHHCCCCCCCCCCCEEEECCCHHH
ALAAEVKAFDGLPATSVDFVLGNTKARMRMVAQYTVAGAYHGLVIGTDHAAEAVMGFFTK
HHHHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHH
FGDGACDLAPLSGLVKNQVRAIARHFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
IDAFLHGEPVREEAFRIICETYAKTQHKRELPYAP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MHAVQRQIAEQLKVQPPFADQNALQAEVARRVNFIKECLQNARLKTLVLGISGGVDSLTA
CCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHH
GLLAQRAVKELRESTGDNAYRFIAVRLPYVVQADEHEAQASVDFIEPDERHTINIGSSVK
HHHHHHHHHHHHHCCCCCCEEEEEEECCEEEECCCCHHHCCCCCCCCCCCEEEECCCHHH
ALAAEVKAFDGLPATSVDFVLGNTKARMRMVAQYTVAGAYHGLVIGTDHAAEAVMGFFTK
HHHHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHH
FGDGACDLAPLSGLVKNQVRAIARHFGAPESLVEKVPTADLEDLSPGKPDEASHGVTYAE
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
IDAFLHGEPVREEAFRIICETYAKTQHKRELPYAP
HHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA