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The map label for this gene is ygbJ [H]

Identifier: 66043733

GI number: 66043733

Start: 504830

End: 505735

Strand: Reverse

Name: ygbJ [H]

Synonym: Psyr_0466

Alternate gene names: 66043733

Gene position: 505735-504830 (Counterclockwise)

Preceding gene: 66043734

Following gene: 66043732

Centisome position: 8.3

GC content: 62.69

Gene sequence:

>906_bases
ATGACTGGCAAAAACGTTGGCGTTATCGGCCTGGGTGCGATGGGTCTGGGCATTGCCCGCTCGTTGCTGCGTAGCGGCTT
CACTGTGCATGCCTGTGATGTGCGCACCAGCGTGACCGAAGCATTCGCCCAGGAAGGCGGGGTGGCCTGTGATTCCCCGG
CCAGCATGGCCGCTGCCTGCGATGTGATCATCACAGTCGTAGTCAATGCCGAGCAGACCGAAACCGTGCTGTTTGGCGAA
AACGGCGCCATTGCCGCGCTGCGTCCGGGCTGCCTGGTCATCGGTTGCGCCACCGTCGCGCCCACCTTCGCCGTCGAACT
GGGCGAGCGTCTGGCTGCACAGAACCTGCTGTACCTCGATGCGCCGATCTCCGGCGGTGCCGCCAAGGCGGCCGCTGGCC
AGATGACCATGATGACCTCCGGCCCGGCCGAGTCCTACGCCAAGGCAGAAGCCATCCTCAACGGCATGGCCGGTAAGGTC
TATCGCCTGGGCGACGTCCACGGTCTGGGCTCGAAAGTCAAAATCATCAATCAACTGCTCGCGGGCGTACACATCGCCGC
CAGCGCCGAAGCCATGGCTCTGGGCCTGCGCGAAGGTGTCGATGCTGACGCGCTGTATGAAGTGATCACCAACAGTGCCG
GTAACTCGTGGATGTTCGAGAACCGCGTGCCGCACATCCTCAATGCCGATTACACGCCGCTGTCGGCCGTGGACATTTTC
GTCAAGGACCTCGGCCTGGTGCTGGATACCGCACGCAGCAGCAAATTCCCGCTGCCACTCTCGGCCACCGCGCACCAGAT
GTTCATGCAGGCATCCAGCGCCGGCTTCGGTCGCGAGGATGACTCGGCCGTGATCAAGATTTTCCCGGGCATCGAGTTGC
CGAAAGCCAAGCCAGAAAAAGCCTGA

Upstream 100 bases:

>100_bases
CATTATTTACTCATTGATTCACTATCCGATTCACAGATCAGAACAATAACGTCCGAATGTCGGCTTCACAGGCATCGGCA
CTGACTTCGAGGATTACTGC

Downstream 100 bases:

>100_bases
GGAGCGCCTGATGAGCCTGAATAACGCTCGCCCGCTGCTGGGCTGCATTGCCGATGACTTCACCGGCGCCACCGACCTGG
CCAACATGCTGGTGCGGGGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MTGKNVGVIGLGAMGLGIARSLLRSGFTVHACDVRTSVTEAFAQEGGVACDSPASMAAACDVIITVVVNAEQTETVLFGE
NGAIAALRPGCLVIGCATVAPTFAVELGERLAAQNLLYLDAPISGGAAKAAAGQMTMMTSGPAESYAKAEAILNGMAGKV
YRLGDVHGLGSKVKIINQLLAGVHIAASAEAMALGLREGVDADALYEVITNSAGNSWMFENRVPHILNADYTPLSAVDIF
VKDLGLVLDTARSSKFPLPLSATAHQMFMQASSAGFGREDDSAVIKIFPGIELPKAKPEKA

Sequences:

>Translated_301_residues
MTGKNVGVIGLGAMGLGIARSLLRSGFTVHACDVRTSVTEAFAQEGGVACDSPASMAAACDVIITVVVNAEQTETVLFGE
NGAIAALRPGCLVIGCATVAPTFAVELGERLAAQNLLYLDAPISGGAAKAAAGQMTMMTSGPAESYAKAEAILNGMAGKV
YRLGDVHGLGSKVKIINQLLAGVHIAASAEAMALGLREGVDADALYEVITNSAGNSWMFENRVPHILNADYTPLSAVDIF
VKDLGLVLDTARSSKFPLPLSATAHQMFMQASSAGFGREDDSAVIKIFPGIELPKAKPEKA
>Mature_300_residues
TGKNVGVIGLGAMGLGIARSLLRSGFTVHACDVRTSVTEAFAQEGGVACDSPASMAAACDVIITVVVNAEQTETVLFGEN
GAIAALRPGCLVIGCATVAPTFAVELGERLAAQNLLYLDAPISGGAAKAAAGQMTMMTSGPAESYAKAEAILNGMAGKVY
RLGDVHGLGSKVKIINQLLAGVHIAASAEAMALGLREGVDADALYEVITNSAGNSWMFENRVPHILNADYTPLSAVDIFV
KDLGLVLDTARSSKFPLPLSATAHQMFMQASSAGFGREDDSAVIKIFPGIELPKAKPEKA

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=31.7241379310345, Blast_Score=153, Evalue=2e-37,
Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=25.0871080139373, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1789092, Length=296, Percent_Identity=53.7162162162162, Blast_Score=289, Evalue=1e-79,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=32.1428571428571, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1790315, Length=282, Percent_Identity=31.5602836879433, Blast_Score=137, Evalue=6e-34,
Organism=Escherichia coli, GI1786719, Length=281, Percent_Identity=26.6903914590747, Blast_Score=114, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=33.3333333333333, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24655230, Length=297, Percent_Identity=29.2929292929293, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI19922568, Length=297, Percent_Identity=29.2929292929293, Blast_Score=144, Evalue=9e-35,
Organism=Drosophila melanogaster, GI28574115, Length=284, Percent_Identity=25, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 30995; Mature: 30864

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGKNVGVIGLGAMGLGIARSLLRSGFTVHACDVRTSVTEAFAQEGGVACDSPASMAAAC
CCCCCEEEEEECHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHH
DVIITVVVNAEQTETVLFGENGAIAALRPGCLVIGCATVAPTFAVELGERLAAQNLLYLD
HEEEEEEECCCCCEEEEECCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEE
APISGGAAKAAAGQMTMMTSGPAESYAKAEAILNGMAGKVYRLGDVHGLGSKVKIINQLL
CCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
AGVHIAASAEAMALGLREGVDADALYEVITNSAGNSWMFENRVPHILNADYTPLSAVDIF
HHHEHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHHCCCCCCHHHHHHH
VKDLGLVLDTARSSKFPLPLSATAHQMFMQASSAGFGREDDSAVIKIFPGIELPKAKPEK
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCC
A
C
>Mature Secondary Structure 
TGKNVGVIGLGAMGLGIARSLLRSGFTVHACDVRTSVTEAFAQEGGVACDSPASMAAAC
CCCCEEEEEECHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCHHHHHHH
DVIITVVVNAEQTETVLFGENGAIAALRPGCLVIGCATVAPTFAVELGERLAAQNLLYLD
HEEEEEEECCCCCEEEEECCCCCEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEE
APISGGAAKAAAGQMTMMTSGPAESYAKAEAILNGMAGKVYRLGDVHGLGSKVKIINQLL
CCCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHH
AGVHIAASAEAMALGLREGVDADALYEVITNSAGNSWMFENRVPHILNADYTPLSAVDIF
HHHEHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEECCCCCHHHCCCCCCHHHHHHH
VKDLGLVLDTARSSKFPLPLSATAHQMFMQASSAGFGREDDSAVIKIFPGIELPKAKPEK
HHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]