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The map label for this gene is entF [C]

Identifier: 66043701

GI number: 66043701

Start: 472788

End: 474479

Strand: Reverse

Name: entF [C]

Synonym: Psyr_0434

Alternate gene names: 66043701

Gene position: 474479-472788 (Counterclockwise)

Preceding gene: 66043702

Following gene: 66043700

Centisome position: 7.79

GC content: 66.84

Gene sequence:

>1692_bases
ATGGATCGCATGAACTGGTTGAACCTGGAACACCTGCTGCTCGATGCGCTGCCCGACCGGCTGCTGACGCTGGCCCCGAC
CCTGGACCACGCGCAGTTTCGTGATCAGGCGCTCAGCGTGGCCGCTGGGCTGCAAGCGCGTGGCATCACCCACCTTGCAG
TGCACCTCGAAGATGCCGCCGACCTGGCCATTGCGTTGTTCGCGGCCTGGCGCGCGGACGTGCATGTGTTGCTGCCCGCC
GACTTGCAGGGCCAGACCCGCGAGCGCTGGGCGAATCAGGTCGACCTTTGGCTGACCGACCTGCCAGGTGACACCCAGCT
GAGCGATCTGCGTGCCGCGCCCCTCCCGGCGGCAGCGCTGGACCTGGACCAATGCCGGCTGAGCCTGTGCACCTCAGGCT
CCAGCGGCGAGCCCAAACTGATCGAGAAGCGCCTGCGGCAACTGGCCAACGAAGTCTGCGGGCTGGAACAGCTGTGGGGC
GCGGAGCTCGGCTCGGCATGCATGATCGGCAGCGTGGCGACGCAACATATTTACGGGCTGCTGTTTCGTTTGCTGTGGCC
GCTGTGCGCGGCACGCCCGTTCGTGCGCCGTCAGTTGCCGTTCGCCGAAGACCTGCAACGCGCCAGCCGTGAATACCCGT
CATTCGCCTGGGTCGCCAGCCCCGCGCTGCTCAAACGCATGGGCGACAATCTCGACTGGCACAGTCTTGCGGCCGTGCGC
CGGGTGTTCTCTTCCGGTGGTGCATTGCCGGCCGACGCCACACAGAGCCTGCAGCAACGCCTCGGCCAGTGGCCGACGGA
AATTCTCGGCAGCTCGGAAACCGGCGGCATCGCCTGGCGTCAGGGCGGGCAGCGCTGGCAGGCCTTCGACGGCGTGGAGC
TGAGTCAGGATGCGCAAGGTGCGCTGCGCATCAGCTCGCCCTATCTGCCGCCCGGCCATGTCGAGCAGACCGCCGACGCG
GCGCTGATCGGCGAAGATGGGCGCTTTGAACTGCTGGGCAGGCTTGACCGCATCGTCAAACTCGAAGAAAAGCGCGTGTC
CTTGCCGCTGATCGAGCAGGCCCTCGCGGCTCACGAATGGGTGAGTGAAGCGCGCCTTGGCGTGGTGCAGGCAAACCGTG
CCTCGCTGGGCGCGCTGCTGGTGCTGAGCGACGCGGGCTTGCTGGCCCTGCGCAATCAGGGCCGTCGGGCGCTCACCGAA
GCCTTGCGACAGCACCTGCAGCCGCATTGCGAAACCATCGCCCTGCCGCGTCGCTGGCGGCTGCTGCGGCAGATGCCGCT
CAACGCCCAGGGCAAGCTGCCTCAAGCAGACGTCGAGGCGCTGCTGCTGGCGCCACGCTCGAAACAACCGGAAGTGCTTG
AGCAACAGAACGTCGAAGGCGAGCTGCACCTGCACTTGAGTGTCCCGCCCGATCTGGCGTTTTTCAGCGGCCACTTTCCC
AAGGCGCCGATTTTGCCGGGCGTGGTGCAGGTCGACTGGGCGATCAGTCTGGGCCGGCGCCTGCTCGACCTGCCATGCGG
ATTCGCCGGCATGGAGGTGCTCAAATTCCAGCAGCTGGTGCGCCCCGGCGACCGTCTCACGCTGACGCTGCGCTTCGATG
CGGCACGCTCCAAGCTGCACTTCGCCTTTCGCAACGCCGACAACGCGCCCTGCTCCAGTGGCCGGATCCTCCTGGAGGAC
GACCATGCATAA

Upstream 100 bases:

>100_bases
TGGTGGACGCTGTACACCGGCCTGATTTCCTACGGCCTGATGGGCGTGTTGTTCGCTGTCGAATGGATCGTCCGCCCACC
CTCTGCGGGCGGCAAATGAA

Downstream 100 bases:

>100_bases
GCCTTGCGTGATCATTCCGGTGTTTGACCACGAGCTGGCCGTGCCCACCGTGGTCAAGGCCGTGCGCAACGCCGGGTTGC
CGTGCATCCTGGTCGACGAT

Product: AMP-dependent synthetase and ligase

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: Thioester Dehydrase Family Protein; Acyl-Co A Synthetase/AMP-(Fatty) Acid Ligase; AMP-(Fatty)Acid Ligase; Acyl-Co A Synthetase; AMP-(Fatty) Acid Ligase; AMP-Ligase; Peptide Synthase; Trigger Factor; Acyl-Co A Synthetase/AMP-(Fatty) Acid Ligase Related Enzyme; Orphan Protein; 3-Phosphoshikimate 1-Carboxyvinyltransferase; CoA Ligase; Acyl-Co A Synthetases/AMP-(Fatty) Acid Ligase; Acyl-CoA Synthetase; Surfactin Synthetase; Acyl-Co A Synthetase-Related Protein; Aconitate Hydratase; Fatty Acid Degradation; Fatty Acid CoA Ligase; Acetyl-Co A Synthetase; (3R)-Hydroxymyristoyl-[Acyl Carrier Protein] Dehydratase

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MDRMNWLNLEHLLLDALPDRLLTLAPTLDHAQFRDQALSVAAGLQARGITHLAVHLEDAADLAIALFAAWRADVHVLLPA
DLQGQTRERWANQVDLWLTDLPGDTQLSDLRAAPLPAAALDLDQCRLSLCTSGSSGEPKLIEKRLRQLANEVCGLEQLWG
AELGSACMIGSVATQHIYGLLFRLLWPLCAARPFVRRQLPFAEDLQRASREYPSFAWVASPALLKRMGDNLDWHSLAAVR
RVFSSGGALPADATQSLQQRLGQWPTEILGSSETGGIAWRQGGQRWQAFDGVELSQDAQGALRISSPYLPPGHVEQTADA
ALIGEDGRFELLGRLDRIVKLEEKRVSLPLIEQALAAHEWVSEARLGVVQANRASLGALLVLSDAGLLALRNQGRRALTE
ALRQHLQPHCETIALPRRWRLLRQMPLNAQGKLPQADVEALLLAPRSKQPEVLEQQNVEGELHLHLSVPPDLAFFSGHFP
KAPILPGVVQVDWAISLGRRLLDLPCGFAGMEVLKFQQLVRPGDRLTLTLRFDAARSKLHFAFRNADNAPCSSGRILLED
DHA

Sequences:

>Translated_563_residues
MDRMNWLNLEHLLLDALPDRLLTLAPTLDHAQFRDQALSVAAGLQARGITHLAVHLEDAADLAIALFAAWRADVHVLLPA
DLQGQTRERWANQVDLWLTDLPGDTQLSDLRAAPLPAAALDLDQCRLSLCTSGSSGEPKLIEKRLRQLANEVCGLEQLWG
AELGSACMIGSVATQHIYGLLFRLLWPLCAARPFVRRQLPFAEDLQRASREYPSFAWVASPALLKRMGDNLDWHSLAAVR
RVFSSGGALPADATQSLQQRLGQWPTEILGSSETGGIAWRQGGQRWQAFDGVELSQDAQGALRISSPYLPPGHVEQTADA
ALIGEDGRFELLGRLDRIVKLEEKRVSLPLIEQALAAHEWVSEARLGVVQANRASLGALLVLSDAGLLALRNQGRRALTE
ALRQHLQPHCETIALPRRWRLLRQMPLNAQGKLPQADVEALLLAPRSKQPEVLEQQNVEGELHLHLSVPPDLAFFSGHFP
KAPILPGVVQVDWAISLGRRLLDLPCGFAGMEVLKFQQLVRPGDRLTLTLRFDAARSKLHFAFRNADNAPCSSGRILLED
DHA
>Mature_563_residues
MDRMNWLNLEHLLLDALPDRLLTLAPTLDHAQFRDQALSVAAGLQARGITHLAVHLEDAADLAIALFAAWRADVHVLLPA
DLQGQTRERWANQVDLWLTDLPGDTQLSDLRAAPLPAAALDLDQCRLSLCTSGSSGEPKLIEKRLRQLANEVCGLEQLWG
AELGSACMIGSVATQHIYGLLFRLLWPLCAARPFVRRQLPFAEDLQRASREYPSFAWVASPALLKRMGDNLDWHSLAAVR
RVFSSGGALPADATQSLQQRLGQWPTEILGSSETGGIAWRQGGQRWQAFDGVELSQDAQGALRISSPYLPPGHVEQTADA
ALIGEDGRFELLGRLDRIVKLEEKRVSLPLIEQALAAHEWVSEARLGVVQANRASLGALLVLSDAGLLALRNQGRRALTE
ALRQHLQPHCETIALPRRWRLLRQMPLNAQGKLPQADVEALLLAPRSKQPEVLEQQNVEGELHLHLSVPPDLAFFSGHFP
KAPILPGVVQVDWAISLGRRLLDLPCGFAGMEVLKFQQLVRPGDRLTLTLRFDAARSKLHFAFRNADNAPCSSGRILLED
DHA

Specific function: Activates The Carboxylate Group Of L-Serine Via ATP- Dependent Ppi Exchange Reactions To The Aminoacyladenylate, Preparing That Molecule For The Final Stages Of Enterobactin Synthesis. Holo-Entf Acts As The Catalyst For The Formation Of The Three Amide An

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 62088; Mature: 62088

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRMNWLNLEHLLLDALPDRLLTLAPTLDHAQFRDQALSVAAGLQARGITHLAVHLEDAA
CCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCHHHCCCEEEEEEECHHH
DLAIALFAAWRADVHVLLPADLQGQTRERWANQVDLWLTDLPGDTQLSDLRAAPLPAAAL
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCHHHH
DLDQCRLSLCTSGSSGEPKLIEKRLRQLANEVCGLEQLWGAELGSACMIGSVATQHIYGL
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHH
LFRLLWPLCAARPFVRRQLPFAEDLQRASREYPSFAWVASPALLKRMGDNLDWHSLAAVR
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCCEEEHHCHHHHHHHCCCCCHHHHHHHH
RVFSSGGALPADATQSLQQRLGQWPTEILGSSETGGIAWRQGGQRWQAFDGVELSQDAQG
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
ALRISSPYLPPGHVEQTADAALIGEDGRFELLGRLDRIVKLEEKRVSLPLIEQALAAHEW
CEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VSEARLGVVQANRASLGALLVLSDAGLLALRNQGRRALTEALRQHLQPHCETIALPRRWR
HHHHHHCEEECCCCCCCEEEEEECCCEEEHHHCCHHHHHHHHHHHCCHHHHEECCHHHHH
LLRQMPLNAQGKLPQADVEALLLAPRSKQPEVLEQQNVEGELHLHLSVPPDLAFFSGHFP
HHHHCCCCCCCCCCHHHHHHHEECCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHCCCCC
KAPILPGVVQVDWAISLGRRLLDLPCGFAGMEVLKFQQLVRPGDRLTLTLRFDAARSKLH
CCCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHE
FAFRNADNAPCSSGRILLEDDHA
EEEECCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MDRMNWLNLEHLLLDALPDRLLTLAPTLDHAQFRDQALSVAAGLQARGITHLAVHLEDAA
CCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHCHHHCCCEEEEEEECHHH
DLAIALFAAWRADVHVLLPADLQGQTRERWANQVDLWLTDLPGDTQLSDLRAAPLPAAAL
HHHHHHHHHHHCCEEEEECCCCCCCHHHHHCCCEEEEEECCCCCCCHHHCCCCCCCHHHH
DLDQCRLSLCTSGSSGEPKLIEKRLRQLANEVCGLEQLWGAELGSACMIGSVATQHIYGL
CHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHH
LFRLLWPLCAARPFVRRQLPFAEDLQRASREYPSFAWVASPALLKRMGDNLDWHSLAAVR
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHCCCCEEEHHCHHHHHHHCCCCCHHHHHHHH
RVFSSGGALPADATQSLQQRLGQWPTEILGSSETGGIAWRQGGQRWQAFDGVELSQDAQG
HHHHCCCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCC
ALRISSPYLPPGHVEQTADAALIGEDGRFELLGRLDRIVKLEEKRVSLPLIEQALAAHEW
CEEECCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
VSEARLGVVQANRASLGALLVLSDAGLLALRNQGRRALTEALRQHLQPHCETIALPRRWR
HHHHHHCEEECCCCCCCEEEEEECCCEEEHHHCCHHHHHHHHHHHCCHHHHEECCHHHHH
LLRQMPLNAQGKLPQADVEALLLAPRSKQPEVLEQQNVEGELHLHLSVPPDLAFFSGHFP
HHHHCCCCCCCCCCHHHHHHHEECCCCCCCHHHHHCCCCCEEEEEEECCCCCHHHCCCCC
KAPILPGVVQVDWAISLGRRLLDLPCGFAGMEVLKFQQLVRPGDRLTLTLRFDAARSKLH
CCCCCCCCEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHE
FAFRNADNAPCSSGRILLEDDHA
EEEECCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA