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The map label for this gene is pilQ [H]

Identifier: 66043674

GI number: 66043674

Start: 436404

End: 438560

Strand: Direct

Name: pilQ [H]

Synonym: Psyr_0407

Alternate gene names: 66043674

Gene position: 436404-438560 (Clockwise)

Preceding gene: 66043673

Following gene: 66043675

Centisome position: 7.16

GC content: 60.64

Gene sequence:

>2157_bases
ATGAAGTGGGCAAGGCCAAGTATGCACAGTAGCGCTCAACGGAAATTGATCATGACCCGGATTCTCTCGATTATCGGCGT
GTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAAGACCCTGGATGTCGCCGCCCTGCCGGGTG
ACCGCATCGAGCTGAAGCTGGCATTCGACGCCCCGGTGCCTGCACCGCGCGGTTACACCACCTCGCAACCGGCGCGCATT
GCGCTGGACCTGCCGGGTGTTACCAGCCAGCTGGCGACCAAAAGTCGTGACCTGGGCACCGGCAATGCGCGCAGTGTGGT
CGTGGTACAGGCTCAGGACCGTACCCGGGTGATCATCAACCTGACCACGCTTTCGCCGTACAGCTCCCGCGTCGATGGCA
ACAACCTGTTCGTGGTCATCGGCCAGGGTGCCGGCGCCGCGCAAGCCTCCCAGCCGACTACCGCGATGGGCGCGCAACGG
GTCGCGGTGCCACCGCCCACACCGGCATCCGCCAAGCCGTTCATCCCCGCTGGCGTCAAGGCCATCAGGAATGTCGACTT
TCAGCGTGGCGAGCTGGGTGAAGGCAATGTGGTCATTGACCTGAGCAACCCCAGCATTGCCCCGGATATTCAGGAGCAGG
GCGGCAAGATCCGCGTGGACTTCGCCAAGACCCAGCTGCCCGAACCGCTGCGCGTCCGCCTGGACGTCAAGGACTTCGCC
ACGCCGGTGCAGTTCGTCAGTGCTTCGGCGACAGGTGACAAGGCCAGCATTCTGATCGAGCCGTCGGGCGCCTTCGACTA
CTCGGCGTTCCAGACCGAAAACAAGCTGACGATCAGCGTGCGCCCGTTGACCAACGAGGATCTGGACCGCCGCGCTGCCG
ACAAGCCGGTGTACACCGGTGAAAAGCTGTCGCTGAACTTCCAGGACATCGATGTGCGTTCGGTGCTGCAGCTGATTGCC
GACTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTCCAGGGCGGTATCACCCTGCGTCTGCAGAATGTGCCATGGGA
TCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGCCTGGACAAGCGCAAGGTTGGCAACGTTCTGCTGGTCGCACCAGCCG
ATGAAATTGCCGCCCGTGAGCGTCAGGAGCTGGAATCGCTCAAACAGATCGCCGAGCTGGCGCCCCTGCGCCGCGAACTG
CTGCAGGTCAATTACGCCAAGGCAGCCGACATCGCCAAGCTGTTCCAGTCCGTGACCAGCGCCGAGTCCAAGGCCGACGA
GCGCGGCTCGATCACGGTGGACGACCGGACCAACAACATCATTGCCTACCAGACCCAGGAACGACTCGACGAACTGCGTC
GTATCGTGTCGCAACTGGACATCCCGGTACGCCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAACGTCGATTACAAC
AAGCAGCTCGGGGTACGCTGGGGCGGGTCTACCAACACCAGCGGCAGCGGAAAATGGACAACCTACGGGCTTGACAATAA
TGGCGACGAGGCCGGTAACACCAGCGGCAACCTGACCCCCAACGTGCCGTTCGTGGACCTCGGCGCAGCCGGCGCTACCT
CGGGTATCGGCCTTGGCTTCGTGACCAACAACACGCTGCTGGACCTTGAGCTGAGTGCCATGGAGAAAACCGGTAACGGT
GAAATCGTCTCCCAGCCCAAGGTGGTGACCTCCGACAAGGAAACCGCCAAGATCCTCAAGGGCACGGAGATCCCGTATCA
GGAATCCAGCTCCAGCGGTGCGACCACCGTGAGCTTCAAGGAAGCTTCCCTGTCGCTTGAAGTGACGCCGCAGATCACGC
CGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGACTACCTGAATGCGGTACTGGGCGTACCGCCC
ATCAAGAAGAACGAGGTCAACGCCAAGGTGCTGATTTCCGACGGCGAAACCATCGTCATCGGTGGTGTGTTCTCGAATAC
GCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCTTGGCCGGCTTTTCAGGCGCGATGTGGTAT
CCGAGTCCAAATCCGAGCTGTTGGTATTTTTGACTCCGCGTATCATGAACAACCAGGCGATTTCTGTGAGTCGTTGA

Upstream 100 bases:

>100_bases
TGTCGATCGGCGACGCGCAGGTTGATGTCATCGAAATTGTTCCTGATGGAGAGGGGGCGTGGCTGGAAAGGCCGCGCACG
ATCGCCCTCAAAGAACGCTC

Downstream 100 bases:

>100_bases
TTTTGTGCGAAATTTAATACTTGTGGGGCCGATGGGTGCTGGCAAGAGCACCATCGGCCGTTTGCTTGCCAAAGAATTGC
GACTGCCGTTCAAGGATTCC

Product: type II and III secretion system protein:NolW-like:NolW-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 718; Mature: 718

Protein sequence:

>718_residues
MKWARPSMHSSAQRKLIMTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARI
ALDLPGVTSQLATKSRDLGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQR
VAVPPPTPASAKPFIPAGVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFA
TPVQFVSASATGDKASILIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIA
DFTNLNLVASDTVQGGITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRREL
LQVNYAKAADIAKLFQSVTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYN
KQLGVRWGGSTNTSGSGKWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNG
EIVSQPKVVTSDKETAKILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPP
IKKNEVNAKVLISDGETIVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Sequences:

>Translated_718_residues
MKWARPSMHSSAQRKLIMTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARI
ALDLPGVTSQLATKSRDLGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQR
VAVPPPTPASAKPFIPAGVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFA
TPVQFVSASATGDKASILIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIA
DFTNLNLVASDTVQGGITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRREL
LQVNYAKAADIAKLFQSVTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYN
KQLGVRWGGSTNTSGSGKWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNG
EIVSQPKVVTSDKETAKILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPP
IKKNEVNAKVLISDGETIVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR
>Mature_718_residues
MKWARPSMHSSAQRKLIMTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTSQPARI
ALDLPGVTSQLATKSRDLGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQR
VAVPPPTPASAKPFIPAGVKAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFA
TPVQFVSASATGDKASILIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIA
DFTNLNLVASDTVQGGITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRREL
LQVNYAKAADIAKLFQSVTSAESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYN
KQLGVRWGGSTNTSGSGKWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGFVTNNTLLDLELSAMEKTGNG
EIVSQPKVVTSDKETAKILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPP
IKKNEVNAKVLISDGETIVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Specific function: Essential for the formation of pili. Involved in the biogenesis of type 4 fimbriae probably by serving as a "porthole" allowing passage of the fimbrae through the outer membrane [H]

COG id: COG4796

COG function: function code U; Type II secretory pathway, component HofQ

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI1789793, Length=422, Percent_Identity=37.2037914691943, Blast_Score=244, Evalue=2e-65,
Organism=Escherichia coli, GI87082242, Length=373, Percent_Identity=27.3458445040214, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR011662
- InterPro:   IPR004846
- InterPro:   IPR004845 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N; PF07660 STN [H]

EC number: NA

Molecular weight: Translated: 77381; Mature: 77381

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWARPSMHSSAQRKLIMTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEE
AFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRDLGTGNARSVVVVQAQDRTRVIIN
EECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEE
LTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPAGVK
EEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCEEECCCCCCCCCCCCCHHHHH
AIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFA
HHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEEEEEHHCCCCCCEEEEEEHHHHC
TPVQFVSASATGDKASILIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTG
CHHHHEECCCCCCCEEEEECCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHCCCCCCCC
EKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQALDLVLKTKGLD
CEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCC
KRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
CHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYN
HHHCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCC
KQLGVRWGGSTNTSGSGKWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGF
CEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEE
VTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQESSSSGATTVSFK
EECCEEEEEEEEHHHCCCCCCEEECCEEEECCHHHHHHHCCCCCCCCCCCCCCCEEEEEE
EASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIVI
ECEEEEEECCEECCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCEEEE
GGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR
EEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECC
>Mature Secondary Structure
MKWARPSMHSSAQRKLIMTRILSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEE
AFDAPVPAPRGYTTSQPARIALDLPGVTSQLATKSRDLGTGNARSVVVVQAQDRTRVIIN
EECCCCCCCCCCCCCCCCEEEEECCCCHHHHHHCCCCCCCCCCCEEEEEEECCCEEEEEE
LTTLSPYSSRVDGNNLFVVIGQGAGAAQASQPTTAMGAQRVAVPPPTPASAKPFIPAGVK
EEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCEEECCCCCCCCCCCCCHHHHH
AIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFA
HHHCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEEEEEHHCCCCCCEEEEEEHHHHC
TPVQFVSASATGDKASILIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTG
CHHHHEECCCCCCCEEEEECCCCCCCCCEEECCCEEEEEEECCCCCCHHHHHCCCCCCCC
EKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVPWDQALDLVLKTKGLD
CEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHCCCCC
KRKVGNVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQSVTS
CHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AESKADERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYN
HHHCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHCCCCHHHHEEEEEEEEECCCCC
KQLGVRWGGSTNTSGSGKWTTYGLDNNGDEAGNTSGNLTPNVPFVDLGAAGATSGIGLGF
CEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCEEEE
VTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQESSSSGATTVSFK
EECCEEEEEEEEHHHCCCCCCEEECCEEEECCHHHHHHHCCCCCCCCCCCCCCCEEEEEE
EASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGETIVI
ECEEEEEECCEECCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCEEEEEECCCEEEE
GGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR
EEEECCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7901733; 10984043 [H]