Click here to switch to the map view.

The map label for this gene is gshA [H]

Identifier: 66043525

GI number: 66043525

Start: 269298

End: 270905

Strand: Reverse

Name: gshA [H]

Synonym: Psyr_0255

Alternate gene names: 66043525

Gene position: 270905-269298 (Counterclockwise)

Preceding gene: 66043526

Following gene: 66043522

Centisome position: 4.45

GC content: 59.76

Gene sequence:

>1608_bases
GTGAAGGATTACACCTTGAGCGAATTTTTGAACCGCCGTCTTGCCTTGCTCGGCGAGCGCAACAACCTCTCTTTGCTGGA
GCAATGCCTGCACGGTATAGAGCGTGAGTGTCTGCGCGTTACTGCAACGGCAGAACTGGCCTGCACGCCTCATCCGCAAG
CTCTGGGTGCCGCACTGACCAATGGCCAGGTCACGACCGATTATTCCGAATCGCTGCTGGAGTTCATCACACCAGCCTTG
AAGAACCCGGCCGAGACCATCGACAACCTGGACCGTATCCATCGCTTCGTTTACAGCAAGCTGGGCGACGAGCTGCTGTG
GAGCCCGTCGATGCCCTGCCCGTTGCCCGACGAAGAGCACATCCCGATCGCGTATTACGGCACGTCGAACATCGGCAAGC
TCAAGTATGTCTATCGCAAGGGTCTGGCGCTGCGTTATGGCAAGACCATGCAGTGCATTGCCGGCATTCACTACAACTTC
TCGTTGCCGGAAGACGCCTGGGCGCTGCTCAAGCAGACCGAAGACTTTGCAGGCGATGCGCGCGACTACCAGTCGCATTC
CTACATCGCGCTGATTCGTAACTTCCGCCGCTACAGCTGGCTGTTGATGTACCTGTTCGGTGCTTCACCGGCACTCGACG
CGGGGTTCCTGCGCGGCCGCAAGCATCAGCTGGAGCAACACTTCGACGCCGACACCCTGTATCTGCCGTACGCCACCAGT
CTGCGCATGAGCGACCTGGGTTATCAGAGTGACGCCCAGGCCGACCTGACCCCTTGCTACAACGATCTGGTCAGCTACAC
CGACAGCCTGCGCAAGGCCGTGGCCACGCCGTACAAGCCTTACGTCGAGGTCGGCACTCATGACCAGAACGGCGAGTGGG
TGCAGCTCAACACCAACGTGCTGCAGATCGAGAACGAGTACTACTCCAACATTCGCCCCAAGCGCGTGACCTATAGCGGC
GAACGCCCCATTCAGGCGCTGGTGGCCCGTGGTGTGCAGTACGTCGAAGTGCGCTGCCTGGACATCAACCCGTTCCTGCC
GACCGGCATCAGCCTGGAGCAGTCGCGCTTCATCGATGCGTTCGTGCTGTATTGCGCCCTCGAGGAAAGCCAGCAACTGG
CCCGCCACGAGTGCTCCAACGCAAGCTCGAACTTCCTGTCAGTGGTCAAGGAAGGGCGTCGCCCGGGTCTGAGCCTGATG
CGTGACAACCGCCCTGTCGATCTCAAGACCTGGGCAACCGAGCTGATGGAGAAGATCACACCGATTGCCCGCTTGCTCGA
TCAGGCGCAGGGCATCGACGAGCACCTCAAGTCGATTGCCGTGCAACAGGCCAAGATCGACGATACCGCGCTCACACCAT
CTGCTCAGGTGCTGGCCAGCATGGAAGCGCATAACGAGGGCTTTACCGCGTTCTCCCTGCGCCAGAGCCAGGTCCACGCC
GAATACTTCCGCACTCACCCGCTGAGCGCTCAGGAACAGGCAGACTTTGAAGCCCAGGCCAAAACCTCGATTGAGGAACA
GGCCGAGCTGGAAGCGACCGAGGAAGTGGTGGATTTCGACACGTTCGTGGGTTCGTATCAGGCGAGCATTCTGTCGATCA
GTAACTGA

Upstream 100 bases:

>100_bases
ATTGTGAACGGGCGTTACCGACCAACCAGGCGACTACGCCAGTTCCTTTCTTCACCCTTGTAGACCGTCAAGTCCACCCC
CATATCGGGGCGACCGATGC

Downstream 100 bases:

>100_bases
TCGTGCAATTACTGTGGGGAGATTGCTCACGAAGGTGGCGGTGCAGGCGCGACTATCGTGCGGCGCTCTGCGTCGCATGC
CTTTCTGGACGCTCCGCGTC

Product: glutamate--cysteine ligase

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 535; Mature: 535

Protein sequence:

>535_residues
MKDYTLSEFLNRRLALLGERNNLSLLEQCLHGIERECLRVTATAELACTPHPQALGAALTNGQVTTDYSESLLEFITPAL
KNPAETIDNLDRIHRFVYSKLGDELLWSPSMPCPLPDEEHIPIAYYGTSNIGKLKYVYRKGLALRYGKTMQCIAGIHYNF
SLPEDAWALLKQTEDFAGDARDYQSHSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRKHQLEQHFDADTLYLPYATS
LRMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVATPYKPYVEVGTHDQNGEWVQLNTNVLQIENEYYSNIRPKRVTYSG
ERPIQALVARGVQYVEVRCLDINPFLPTGISLEQSRFIDAFVLYCALEESQQLARHECSNASSNFLSVVKEGRRPGLSLM
RDNRPVDLKTWATELMEKITPIARLLDQAQGIDEHLKSIAVQQAKIDDTALTPSAQVLASMEAHNEGFTAFSLRQSQVHA
EYFRTHPLSAQEQADFEAQAKTSIEEQAELEATEEVVDFDTFVGSYQASILSISN

Sequences:

>Translated_535_residues
MKDYTLSEFLNRRLALLGERNNLSLLEQCLHGIERECLRVTATAELACTPHPQALGAALTNGQVTTDYSESLLEFITPAL
KNPAETIDNLDRIHRFVYSKLGDELLWSPSMPCPLPDEEHIPIAYYGTSNIGKLKYVYRKGLALRYGKTMQCIAGIHYNF
SLPEDAWALLKQTEDFAGDARDYQSHSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRKHQLEQHFDADTLYLPYATS
LRMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVATPYKPYVEVGTHDQNGEWVQLNTNVLQIENEYYSNIRPKRVTYSG
ERPIQALVARGVQYVEVRCLDINPFLPTGISLEQSRFIDAFVLYCALEESQQLARHECSNASSNFLSVVKEGRRPGLSLM
RDNRPVDLKTWATELMEKITPIARLLDQAQGIDEHLKSIAVQQAKIDDTALTPSAQVLASMEAHNEGFTAFSLRQSQVHA
EYFRTHPLSAQEQADFEAQAKTSIEEQAELEATEEVVDFDTFVGSYQASILSISN
>Mature_535_residues
MKDYTLSEFLNRRLALLGERNNLSLLEQCLHGIERECLRVTATAELACTPHPQALGAALTNGQVTTDYSESLLEFITPAL
KNPAETIDNLDRIHRFVYSKLGDELLWSPSMPCPLPDEEHIPIAYYGTSNIGKLKYVYRKGLALRYGKTMQCIAGIHYNF
SLPEDAWALLKQTEDFAGDARDYQSHSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRKHQLEQHFDADTLYLPYATS
LRMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVATPYKPYVEVGTHDQNGEWVQLNTNVLQIENEYYSNIRPKRVTYSG
ERPIQALVARGVQYVEVRCLDINPFLPTGISLEQSRFIDAFVLYCALEESQQLARHECSNASSNFLSVVKEGRRPGLSLM
RDNRPVDLKTWATELMEKITPIARLLDQAQGIDEHLKSIAVQQAKIDDTALTPSAQVLASMEAHNEGFTAFSLRQSQVHA
EYFRTHPLSAQEQADFEAQAKTSIEEQAELEATEEVVDFDTFVGSYQASILSISN

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=493, Percent_Identity=41.1764705882353, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 60447; Mature: 60447

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYTLSEFLNRRLALLGERNNLSLLEQCLHGIERECLRVTATAELACTPHPQALGAALT
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHC
NGQVTTDYSESLLEFITPALKNPAETIDNLDRIHRFVYSKLGDELLWSPSMPCPLPDEEH
CCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCC
IPIAYYGTSNIGKLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEDAWALLKQTEDFAGDA
CCEEEECCCCCCHHHHHHHCCCCEECCCHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCC
RDYQSHSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRKHQLEQHFDADTLYLPYATS
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCEEEECCCCC
LRMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVATPYKPYVEVGTHDQNGEWVQLNTNV
CCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEECCE
LQIENEYYSNIRPKRVTYSGERPIQALVARGVQYVEVRCLDINPFLPTGISLEQSRFIDA
EEECHHHHHCCCCCEEEECCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHH
FVLYCALEESQQLARHECSNASSNFLSVVKEGRRPGLSLMRDNRPVDLKTWATELMEKIT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHH
PIARLLDQAQGIDEHLKSIAVQQAKIDDTALTPSAQVLASMEAHNEGFTAFSLRQSQVHA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHH
EYFRTHPLSAQEQADFEAQAKTSIEEQAELEATEEVVDFDTFVGSYQASILSISN
HHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure
MKDYTLSEFLNRRLALLGERNNLSLLEQCLHGIERECLRVTATAELACTPHPQALGAALT
CCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEEEEEECCCHHHHHHHHC
NGQVTTDYSESLLEFITPALKNPAETIDNLDRIHRFVYSKLGDELLWSPSMPCPLPDEEH
CCCEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCC
IPIAYYGTSNIGKLKYVYRKGLALRYGKTMQCIAGIHYNFSLPEDAWALLKQTEDFAGDA
CCEEEECCCCCCHHHHHHHCCCCEECCCHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCC
RDYQSHSYIALIRNFRRYSWLLMYLFGASPALDAGFLRGRKHQLEQHFDADTLYLPYATS
HHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCEEEECCCCC
LRMSDLGYQSDAQADLTPCYNDLVSYTDSLRKAVATPYKPYVEVGTHDQNGEWVQLNTNV
CCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEEEEECCE
LQIENEYYSNIRPKRVTYSGERPIQALVARGVQYVEVRCLDINPFLPTGISLEQSRFIDA
EEECHHHHHCCCCCEEEECCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCCHHHHHHHH
FVLYCALEESQQLARHECSNASSNFLSVVKEGRRPGLSLMRDNRPVDLKTWATELMEKIT
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHHHHHHHH
PIARLLDQAQGIDEHLKSIAVQQAKIDDTALTPSAQVLASMEAHNEGFTAFSLRQSQVHA
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEHHHHHHHH
EYFRTHPLSAQEQADFEAQAKTSIEEQAELEATEEVVDFDTFVGSYQASILSISN
HHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA