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The map label for this gene is spoT [H]

Identifier: 66043479

GI number: 66043479

Start: 223004

End: 225127

Strand: Reverse

Name: spoT [H]

Synonym: Psyr_0209

Alternate gene names: 66043479

Gene position: 225127-223004 (Counterclockwise)

Preceding gene: 66043480

Following gene: 66043478

Centisome position: 3.69

GC content: 60.4

Gene sequence:

>2124_bases
TTGGCAAGGGGTAGCGCCTTGCCGAGCATAGACGCCCTCGCCGACAGACTGTCGGCCTACCTCGGCAAGGACCAGGTCAA
TCTGGTCCGCCGAGCGTATTTCTACGCCGAACAAGCCCACGATGGCCAACGCCGTCGCAGCGGTGAAGCCTACGTCACCC
ATCCTCTCGCGGTGGCGAACATTCTTGCCGATATGCACATGGACCATCAGAGCCTGATGGCTGCGATGCTGCATGACGTC
ATCGAAGACACCGGGATTGCCAAGGAAGCGCTCAGCGCCCAATTTGGCGAGACCGTCGCGGAACTGGTCGACGGGGTCAG
CAAACTGACCCAGATGAATTTCGAGACCAAGGCCGAAGCCCAGGCGGAAAACTTCCAGAAAATGGCCATGGCCATGGCGC
GCGACATTCGCGTCATCCTGGTCAAGCTCGCTGATCGCCTGCACAACATGCGCACGCTGGAAGTCCTGTCCGGCGAAAAG
CGTCGGCGCATCGCCAAGGAAACCCTTGAAATCTATGCGCCCATCGCCAATCGGCTGGGCATGCACAGTGTGCGCATCGA
ATTCGAGGACCTGGGCTTCAAGGCGATGTACCCGATGCGTTCCTCGCGAATCAACCAGGCGGTCAAGCGCGCCAGGGGCA
ATCGCAAGGAACTGGTCAACAAGATCGAAGAGTCCCTGAGTCATTGCCTGGCCGTCGATGGCATCGAAGGCGACGTCAGT
GGGCGGCAGAAACATCTGTATGGCATCTACAAGAAGATGCGCGGCAAGCGTCGCGCCTTCAACGAGATCATGGATGTCTA
CGCGTTCCGCATCATCGTCGACAAGGTCGACACCTGCTATCGCGTGCTCGGCGCTGTACATAATCTGTACAAGCCGCTGC
CCGGTCGCTTCAAGGATTACATCGCGATCCCCAAGGCCAACGGCTATCAGTCCCTGCACACGACATTGTTCGGCATGCAT
GGCGTGCCTATCGAGATCCAGATTCGCACCCGCGAAATGGAAGAAATGGCCAACAACGGTATCGCGGCACACTGGCTGTA
CAAGTCCAGTGACGACGAGCAGCCCAAGGGCACTCACGCCCGGGCCCGACAATGGGTCAAGGGCGTGCTGGAAATGCAGC
AGCGCGCCGGCAACTCGCTGGAATTCATCGAAAGCGTCAAGATCGACCTGTTCCCGGACGAGGTCTACGTTTTCACGCCC
AAAGGCCGGATCATGGAGCTGCCCAAAGGCTCCACAGCGGTGGATTTCGCCTACGCGGTACACACCGATGTCGGCAACAG
CTGCATTGCCTGCCGGATCAACCGCCGGCTGGCGCCGCTTTCCGAACCGCTGCAAAGTGGCTCGACCGTCGAGATCGTCA
GCGCTCCAGGCGCACGCCCGAACCCGGCCTGGCTGAACTTTGTCGTCACCGGCAAGGCGCGTACCCACATTCGTCACGCA
CTCAAGCTGCAACGCCGTTCCGAGTCGATCAATCTGGGCGAGCGCCTGCTGAACAAGGTCCTCAACGGGTTTGAGTGCAG
CCTCGACAAGATCGGTCAGGAGCGCATTCAACTGGTGCTCAACGAATACCGGGTCGAAGTGCTGGAAGACCTGCTCGAAG
ACATCGGCCTGGGTAATCGCATGGCTTATGTCGTTGCCCGCCGCCTGCTGGGCGAAGGCGATCAGTTGCCAAGCCCTGAA
GGCCCGCTGGCGATTCGTGGCACCGAAGGCCTGGTGCTCAGCTACGCGAAATGCTGCACGCCAATCCCGGGCGACCCGAT
TGTCGGCCACCTGTCTGCAGGCAAGGGCATGGTCGTGCACCTGGACAACTGCCGCAACATCAGCGAAATCCGCCACAACC
CGGAAAAATGCATCCAGCTGTCCTGGGCCAAGGACGTGACGGGCGAATTCAACGTCGAACTGCGCGTCGAACTCGAGCAT
CAGCGCGGCCTGATCGCGCTGCTGGCCAGCAGCGTCAACGCGGCGGACGGCAATATCGAAAAAATCAGCATGGACGAACG
CGATGGTCGCATCAGCGTGGTCCAACTGGTGGTCAGCGTGCACGATCGCGTGCACCTGGCTCGTGTGATCAAGAAACTGC
GCGCCCTGACCGGTGTCACACGCATCACCCGGATGCGCGCGTAG

Upstream 100 bases:

>100_bases
CGTTGAAGATGAGCCATTGTTCGCAGCGTTCGAGGACGAGTCCAACGAGGCCGTCTAAGCCTATGCCAGGTCGACGTAGT
ACGGCGCAGGGTAAAAGCCA

Downstream 100 bases:

>100_bases
GCCATCCCACTATCAGGAGTTCTCATGACCAAGACTGTCATCACCAGCGATAAAGCCCCTGCGGCCATTGGCCCTTACTC
ACAGGCCATCAAAGCTGGCA

Product: RelA/SpoT protein

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MARGSALPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDV
IEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEK
RRRIAKETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVS
GRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMH
GVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTP
KGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHA
LKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPE
GPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEH
QRGLIALLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA

Sequences:

>Translated_707_residues
MARGSALPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDV
IEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEK
RRRIAKETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVS
GRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMH
GVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTP
KGRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHA
LKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPE
GPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEH
QRGLIALLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA
>Mature_706_residues
ARGSALPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMHMDHQSLMAAMLHDVI
EDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKR
RRIAKETLEIYAPIANRLGMHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVSG
RQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDYIAIPKANGYQSLHTTLFGMHG
VPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHARARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPK
GRIMELPKGSTAVDFAYAVHTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHAL
KLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNRMAYVVARRLLGEGDQLPSPEG
PLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVHLDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQ
RGLIALLASSVNAADGNIEKISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=700, Percent_Identity=54, Blast_Score=793, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=684, Percent_Identity=31.4327485380117, Blast_Score=381, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25143118, Length=131, Percent_Identity=33.587786259542, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79154; Mature: 79022

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGSALPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVAN
CCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEECHHHHHH
ILADMHMDHQSLMAAMLHDVIEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEA
HHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
MHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVS
CEEEEEEHHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCC
GRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDY
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHE
IAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHA
EECCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
RARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAV
HHHHHHHHHHHHHHHCCCCHHHHHHCEEEEECCCEEEECCCCCEEECCCCCCCEEEEEEE
HTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHA
ECCCCCCEEEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHH
LKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNR
HHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
MAYVVARRLLGEGDQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVH
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEE
LDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIE
ECCCCCHHHHHCCHHHHEEEEHHHCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCEE
KISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARGSALPSIDALADRLSAYLGKDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVAN
CCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHCCCCCEEECHHHHHH
ILADMHMDHQSLMAAMLHDVIEDTGIAKEALSAQFGETVAELVDGVSKLTQMNFETKAEA
HHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
QAENFQKMAMAMARDIRVILVKLADRLHNMRTLEVLSGEKRRRIAKETLEIYAPIANRLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
MHSVRIEFEDLGFKAMYPMRSSRINQAVKRARGNRKELVNKIEESLSHCLAVDGIEGDVS
CEEEEEEHHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCC
GRQKHLYGIYKKMRGKRRAFNEIMDVYAFRIIVDKVDTCYRVLGAVHNLYKPLPGRFKDY
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHE
IAIPKANGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSSDDEQPKGTHA
EECCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCHH
RARQWVKGVLEMQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMELPKGSTAVDFAYAV
HHHHHHHHHHHHHHHCCCCHHHHHHCEEEEECCCEEEECCCCCEEECCCCCCCEEEEEEE
HTDVGNSCIACRINRRLAPLSEPLQSGSTVEIVSAPGARPNPAWLNFVVTGKARTHIRHA
ECCCCCCEEEEEECCCCCCHHHHHHCCCEEEEEECCCCCCCCCEEEEEEECCHHHHHHHH
LKLQRRSESINLGERLLNKVLNGFECSLDKIGQERIQLVLNEYRVEVLEDLLEDIGLGNR
HHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
MAYVVARRLLGEGDQLPSPEGPLAIRGTEGLVLSYAKCCTPIPGDPIVGHLSAGKGMVVH
HHHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCEEEECCCCCEEEE
LDNCRNISEIRHNPEKCIQLSWAKDVTGEFNVELRVELEHQRGLIALLASSVNAADGNIE
ECCCCCHHHHHCCHHHHEEEEHHHCCCCCEEEEEEEEECCCCCHHHHHHHHCCCCCCCEE
KISMDERDGRISVVQLVVSVHDRVHLARVIKKLRALTGVTRITRMRA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]