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The map label for this gene is glnK [H]

Identifier: 66043460

GI number: 66043460

Start: 202013

End: 202351

Strand: Reverse

Name: glnK [H]

Synonym: Psyr_0190

Alternate gene names: 66043460

Gene position: 202351-202013 (Counterclockwise)

Preceding gene: 66043463

Following gene: 66043459

Centisome position: 3.32

GC content: 52.8

Gene sequence:

>339_bases
ATGAAGCTAGTCACTGCCATCATCAAGCCGTTCAAACTGGATGACGTTCGCGAGTCGTTGTCTGAAATCGGCGTGCAGGG
CATCACCGTCACTGAAGTCAAAGGCTTCGGGCGTCAGAAGGGTCACACTGAGCTTTATCGCGGTGCTGAATACGTCGTCG
ACTTCCTTCCCAAAGTGAAGATCGATGTCGCCATCGATGACAAGGATCTGGATCGTGTCATCGAAGCCATAACCAAGGCC
GCCAACACTGGCAAGATCGGTGACGGCAAGATTTTCGTGGTCAATCTGGAACAGGCCATCCGCATCCGTACCGGCGAGAC
CGATACAGACGCTATTTAA

Upstream 100 bases:

>100_bases
GATCAGGCGGCCGACACGTTACAAAGCCAGACACTGCGCTTAGACTTGAGTCGGGTTTGTTTTCCTGGGGCAAGTCCACC
AAATTCGGGAGAAAGTTTTC

Downstream 100 bases:

>100_bases
GCCGCCAAACCCAACGCCCCAGGAGAAAACAATATGACTCTGCGTCAATTCGCAGGGCTCGGTGCCCTGTTGTCCCTCGC
ATTACCTGGTCTTGCCCTGG

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIDVAIDDKDLDRVIEAITKA
ANTGKIGDGKIFVVNLEQAIRIRTGETDTDAI

Sequences:

>Translated_112_residues
MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIDVAIDDKDLDRVIEAITKA
ANTGKIGDGKIFVVNLEQAIRIRTGETDTDAI
>Mature_112_residues
MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVKIDVAIDDKDLDRVIEAITKA
ANTGKIGDGKIFVVNLEQAIRIRTGETDTDAI

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=75.8928571428571, Blast_Score=172, Evalue=4e-45,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=67.8571428571429, Blast_Score=156, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12330; Mature: 12330

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
IDVAIDDKDLDRVIEAITKAANTGKIGDGKIFVVNLEQAIRIRTGETDTDAI
EEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEEECCCCCCCCC
>Mature Secondary Structure
MKLVTAIIKPFKLDDVRESLSEIGVQGITVTEVKGFGRQKGHTELYRGAEYVVDFLPKVK
CCHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
IDVAIDDKDLDRVIEAITKAANTGKIGDGKIFVVNLEQAIRIRTGETDTDAI
EEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]