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The map label for this gene is amtB [H]

Identifier: 66043459

GI number: 66043459

Start: 200642

End: 201979

Strand: Reverse

Name: amtB [H]

Synonym: Psyr_0189

Alternate gene names: 66043459

Gene position: 201979-200642 (Counterclockwise)

Preceding gene: 66043460

Following gene: 66043458

Centisome position: 3.31

GC content: 61.21

Gene sequence:

>1338_bases
ATGACTCTGCGTCAATTCGCAGGGCTCGGTGCCCTGTTGTCCCTCGCATTACCTGGTCTTGCCCTGGCGGCTGATCCAGT
GGCCGAGCCGGTGCTGAACTCTGGCGACACTGCATGGATGCTGACCTCGACCGCTCTGGTCCTGTTCATGACCATCCCCG
GTCTCGCGCTGTTCTACGGCGGCATGGTTCGTTCCAAGAACATTCTGTCGGTGATGATGCAGTGCTTCGCCATCACCGGT
CTGATCAGCATCCTGTGGGTCGTCTACGGCTACAGCATCGCGTTTGACACAACCGGTATGGAAGCGGGCGTCGTCAACTT
CAATTCCTTCTTTGGCGGTATGGGTAAAGCGTTCCTGGCAGGCATCACGCCGGCAAGCATTACCGGTCCTGCTGCGTTGT
TCCCTGAGGCGGTGTTCGTCACCTTCCAGATGACCTTCGCGATCATCACCCCTGCCCTAATTGTCGGCGCCTTCGCAGAA
CGCATGAAGTTCTCCGCGATGCTGGTGTTCATGGGTATCTGGTTCACTCTGGTCTACGCGCCAATCGCCCACATGGTCTG
GGGCGGCGTCGGTGGCCTGTTGTGGGACTGGGGCGTTCTGGACTTCGCTGGCGGTACCGTCGTACATATCAACGCCGGTG
TAGCCGGTCTTGTAGCGTGCCTGGTACTGGGCAAGCGCAAAGGCTTCCCGACCACGCCGATGGCGCCGCACAACCTGGGT
TACACCCTGATCGGCGCAGCGATGCTCTGGGTCGGCTGGTTCGGCTTCAACGCCGGTTCCGCCGCTGCGGCCAACGGTAC
TGCCGGTATGGCCATGCTGGTCACTCAAATCGCTACCGCGGCCGCCGCATTGGGCTGGATGTTTGCCGAGTGGCTGACTC
ACGGTAAGCCAAGTGCTCTGGGTATTGCCTCTGGTGTGGTTGCCGGCCTGGTTGCCGTTACCCCTGCGGCCGGTACCGTC
GGCCCGATGGGCGCGCTGATCATCGGTCTGGCAGCAGGCGTGATCTGCTTCTTCTGCGCGACCAGCCTCAAGCGCAAGCT
GGGCTACGACGACTCCCTGGATGCGTTCGGCGTTCACGGCATCGGCGGTATCGTCGGCGCCATCCTGACCGGTGTGTTCG
CAGCACCTGCACTGGGCGGCTTCGGCACCGTGACCGACATCGGTGCTCAGGTCTGGATTCAGTTCAAGGGCGTCGCGTTC
ACCGTTGTCTACACCGCAATCGTGACCTTCATCATCCTCAAGGTGCTGGATGCGGTCATGGGTCTGCGCGTCACCGACGA
GGAAGAGTCTGTCGGTCTGGACCTTGCGCAACACAACGAGCGTGGCTACAACTTGTAA

Upstream 100 bases:

>100_bases
TTTCGTGGTCAATCTGGAACAGGCCATCCGCATCCGTACCGGCGAGACCGATACAGACGCTATTTAAGCCGCCAAACCCA
ACGCCCCAGGAGAAAACAAT

Downstream 100 bases:

>100_bases
GCTTTGGCCTGCGCAAAAAAGAATGCCCGGTCTCCGGGCATTTTTTTTGTCTGACGTTTGTGGATATACCGCCGCTTTTT
GCTGTCATTGATCTTGCCCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTLRQFAGLGALLSLALPGLALAADPVAEPVLNSGDTAWMLTSTALVLFMTIPGLALFYGGMVRSKNILSVMMQCFAITG
LISILWVVYGYSIAFDTTGMEAGVVNFNSFFGGMGKAFLAGITPASITGPAALFPEAVFVTFQMTFAIITPALIVGAFAE
RMKFSAMLVFMGIWFTLVYAPIAHMVWGGVGGLLWDWGVLDFAGGTVVHINAGVAGLVACLVLGKRKGFPTTPMAPHNLG
YTLIGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWLTHGKPSALGIASGVVAGLVAVTPAAGTV
GPMGALIIGLAAGVICFFCATSLKRKLGYDDSLDAFGVHGIGGIVGAILTGVFAAPALGGFGTVTDIGAQVWIQFKGVAF
TVVYTAIVTFIILKVLDAVMGLRVTDEEESVGLDLAQHNERGYNL

Sequences:

>Translated_445_residues
MTLRQFAGLGALLSLALPGLALAADPVAEPVLNSGDTAWMLTSTALVLFMTIPGLALFYGGMVRSKNILSVMMQCFAITG
LISILWVVYGYSIAFDTTGMEAGVVNFNSFFGGMGKAFLAGITPASITGPAALFPEAVFVTFQMTFAIITPALIVGAFAE
RMKFSAMLVFMGIWFTLVYAPIAHMVWGGVGGLLWDWGVLDFAGGTVVHINAGVAGLVACLVLGKRKGFPTTPMAPHNLG
YTLIGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWLTHGKPSALGIASGVVAGLVAVTPAAGTV
GPMGALIIGLAAGVICFFCATSLKRKLGYDDSLDAFGVHGIGGIVGAILTGVFAAPALGGFGTVTDIGAQVWIQFKGVAF
TVVYTAIVTFIILKVLDAVMGLRVTDEEESVGLDLAQHNERGYNL
>Mature_444_residues
TLRQFAGLGALLSLALPGLALAADPVAEPVLNSGDTAWMLTSTALVLFMTIPGLALFYGGMVRSKNILSVMMQCFAITGL
ISILWVVYGYSIAFDTTGMEAGVVNFNSFFGGMGKAFLAGITPASITGPAALFPEAVFVTFQMTFAIITPALIVGAFAER
MKFSAMLVFMGIWFTLVYAPIAHMVWGGVGGLLWDWGVLDFAGGTVVHINAGVAGLVACLVLGKRKGFPTTPMAPHNLGY
TLIGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWLTHGKPSALGIASGVVAGLVAVTPAAGTVG
PMGALIIGLAAGVICFFCATSLKRKLGYDDSLDAFGVHGIGGIVGAILTGVFAAPALGGFGTVTDIGAQVWIQFKGVAFT
VVYTAIVTFIILKVLDAVMGLRVTDEEESVGLDLAQHNERGYNL

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=436, Percent_Identity=51.8348623853211, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17550182, Length=454, Percent_Identity=28.4140969162996, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=440, Percent_Identity=27.0454545454545, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=454, Percent_Identity=26.8722466960352, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=446, Percent_Identity=27.3542600896861, Blast_Score=113, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=36.6430260047281, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=35.3488372093023, Blast_Score=225, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=430, Percent_Identity=33.4883720930233, Blast_Score=219, Evalue=6e-58,
Organism=Drosophila melanogaster, GI161078314, Length=427, Percent_Identity=27.1662763466042, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45933; Mature: 45802

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRQFAGLGALLSLALPGLALAADPVAEPVLNSGDTAWMLTSTALVLFMTIPGLALFYG
CCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHC
GMVRSKNILSVMMQCFAITGLISILWVVYGYSIAFDTTGMEAGVVNFNSFFGGMGKAFLA
CHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCEEHHHHHCCHHHHHHH
GITPASITGPAALFPEAVFVTFQMTFAIITPALIVGAFAERMKFSAMLVFMGIWFTLVYA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAHMVWGGVGGLLWDWGVLDFAGGTVVHINAGVAGLVACLVLGKRKGFPTTPMAPHNLG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
YTLIGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWLTHGKPSAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIASGVVAGLVAVTPAAGTVGPMGALIIGLAAGVICFFCATSLKRKLGYDDSLDAFGVHG
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
IGGIVGAILTGVFAAPALGGFGTVTDIGAQVWIQFKGVAFTVVYTAIVTFIILKVLDAVM
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
GLRVTDEEESVGLDLAQHNERGYNL
CCCCCCCHHHCCCCHHHCCCCCCCC
>Mature Secondary Structure 
TLRQFAGLGALLSLALPGLALAADPVAEPVLNSGDTAWMLTSTALVLFMTIPGLALFYG
CHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHC
GMVRSKNILSVMMQCFAITGLISILWVVYGYSIAFDTTGMEAGVVNFNSFFGGMGKAFLA
CHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCCCCCEEHHHHHCCHHHHHHH
GITPASITGPAALFPEAVFVTFQMTFAIITPALIVGAFAERMKFSAMLVFMGIWFTLVYA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAHMVWGGVGGLLWDWGVLDFAGGTVVHINAGVAGLVACLVLGKRKGFPTTPMAPHNLG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
YTLIGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWLTHGKPSAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIASGVVAGLVAVTPAAGTVGPMGALIIGLAAGVICFFCATSLKRKLGYDDSLDAFGVHG
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
IGGIVGAILTGVFAAPALGGFGTVTDIGAQVWIQFKGVAFTVVYTAIVTFIILKVLDAVM
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
GLRVTDEEESVGLDLAQHNERGYNL
CCCCCCCHHHCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]