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The map label for this gene is rimK [H]

Identifier: 66043443

GI number: 66043443

Start: 186867

End: 187772

Strand: Direct

Name: rimK [H]

Synonym: Psyr_0173

Alternate gene names: 66043443

Gene position: 186867-187772 (Clockwise)

Preceding gene: 66043442

Following gene: 66043444

Centisome position: 3.07

GC content: 59.27

Gene sequence:

>906_bases
ATGAAGATCGCTGTGCTTTCGCGTAATCCGCGTCTGTATTCCACCCGTCGTCTGGTCGAAGCTGGCATCGAGCGTGGCCA
TGAGATGGTGGTGATCGACACCCTGCGCGCCTATATGAACATCGCCAGCCACAAGCCGCAGATCCACTACCGCGGCAAAC
CTCTGGAGGGCTTCGATGCGGTGATCCCGCGCATCGGTGCTTCGGTGACTTTCTATGGCTGTGCGGTGTTGCGTCAGTTT
GAAATGATGGGTGTGTTTCCGCTCAATGAATCGGTCGCCATTGCCCGTTCGCGGGACAAGCTGCGTTCGCTGCAACTCCT
GTCACGCCGGGGGATCGGCTTGCCGGTCACCGGTTTCGCTCACTCGCCCGACGACATTCCCGACCTGATCCAGATGGTCA
ACGGCGCGCCGCTGGTCATCAAGGTGCTCGAAGGCACTCAGGGGATCGGCGTGGTGTTGTGCGAAACGGCCACGGCGGCC
GAATCGGTGATCGAAGCCTTCATGGGCCTCAAGCAGGACATCATGGTTCAGGAATACATCAAGGAAGCAGGCGGTGCCGA
CATTCGCTGCTTTGTGGTCGGTGACAAGGTTATCGCCTCGATGAAACGTCAGGCCAAACCGGGCGAGTTCCGCTCCAACC
TGCACCGCGGTGGCAGCGCCAGCCTGATCAAAATCACGCCTGAAGAACGCATGACTGCATTGCGTGCAGCCAAGGTCATG
GGGCTGAGCGTGGCGGGTGTGGATATCCTGCGTTCCAATCACGGTCCTTTGGTGATGGAGGTCAACTCTTCGCCTGGCCT
GGAAGGCATTGAAGTGACCACCAGCAAGGACGTGGCCGGGATGATCATCGGGTACCTGGAGAAAAACAGCGGCCCGCACA
TGACCCGCACCAAAGGAAAAGGCTGA

Upstream 100 bases:

>100_bases
GTTCCAAGGCTCTGATCGACGGCCAGCTGGTGGTCAATCCGGGCATAACTTACGTACAAGACAAGCCGGTGTTCCCGGCC
TCTACTTCCTCAGGTGTTGC

Downstream 100 bases:

>100_bases
CGGGATTCACACCCGTTTCAATGGCTCTGGCTGGCATCGCGTCTGCGGACGCCGGCCAGGGCATTCAGCTACCGTACATC
AGGTAAACAGGCCTGCCAGT

Product: S6 modification enzyme RimK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MKIAVLSRNPRLYSTRRLVEAGIERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDAVIPRIGASVTFYGCAVLRQF
EMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFAHSPDDIPDLIQMVNGAPLVIKVLEGTQGIGVVLCETATAA
ESVIEAFMGLKQDIMVQEYIKEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
GLSVAGVDILRSNHGPLVMEVNSSPGLEGIEVTTSKDVAGMIIGYLEKNSGPHMTRTKGKG

Sequences:

>Translated_301_residues
MKIAVLSRNPRLYSTRRLVEAGIERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDAVIPRIGASVTFYGCAVLRQF
EMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFAHSPDDIPDLIQMVNGAPLVIKVLEGTQGIGVVLCETATAA
ESVIEAFMGLKQDIMVQEYIKEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
GLSVAGVDILRSNHGPLVMEVNSSPGLEGIEVTTSKDVAGMIIGYLEKNSGPHMTRTKGKG
>Mature_301_residues
MKIAVLSRNPRLYSTRRLVEAGIERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDAVIPRIGASVTFYGCAVLRQF
EMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFAHSPDDIPDLIQMVNGAPLVIKVLEGTQGIGVVLCETATAA
ESVIEAFMGLKQDIMVQEYIKEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
GLSVAGVDILRSNHGPLVMEVNSSPGLEGIEVTTSKDVAGMIIGYLEKNSGPHMTRTKGKG

Specific function: Responsible for the addition of glutamate residues to the C-terminus of ribosomal protein S6 [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=216, Percent_Identity=28.2407407407407, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI134254462, Length=216, Percent_Identity=28.2407407407407, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1787076, Length=290, Percent_Identity=66.551724137931, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 32608; Mature: 32608

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLSRNPRLYSTRRLVEAGIERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VIPRIGASVTFYGCAVLRQFEMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFA
HHHHHCCCHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
HSPDDIPDLIQMVNGAPLVIKVLEGTQGIGVVLCETATAAESVIEAFMGLKQDIMVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHH
KEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
HHCCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHH
GLSVAGVDILRSNHGPLVMEVNSSPGLEGIEVTTSKDVAGMIIGYLEKNSGPHMTRTKGK
CCEEECHHEEECCCCCEEEEECCCCCCCCEEEECCCCHHHHHHEEEECCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MKIAVLSRNPRLYSTRRLVEAGIERGHEMVVIDTLRAYMNIASHKPQIHYRGKPLEGFDA
CEEEEEECCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCEEEECCCCCCHHHH
VIPRIGASVTFYGCAVLRQFEMMGVFPLNESVAIARSRDKLRSLQLLSRRGIGLPVTGFA
HHHHHCCCHHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCC
HSPDDIPDLIQMVNGAPLVIKVLEGTQGIGVVLCETATAAESVIEAFMGLKQDIMVQEYI
CCCCCHHHHHHHHCCCCEEEEEECCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHH
KEAGGADIRCFVVGDKVIASMKRQAKPGEFRSNLHRGGSASLIKITPEERMTALRAAKVM
HHCCCCCEEEEEECHHHHHHHHHCCCCCHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHH
GLSVAGVDILRSNHGPLVMEVNSSPGLEGIEVTTSKDVAGMIIGYLEKNSGPHMTRTKGK
CCEEECHHEEECCCCCEEEEECCCCCCCCEEEECCCCHHHHHHEEEECCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA