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The map label for this gene is ycgF [H]

Identifier: 66043441

GI number: 66043441

Start: 185595

End: 186377

Strand: Direct

Name: ycgF [H]

Synonym: Psyr_0171

Alternate gene names: 66043441

Gene position: 185595-186377 (Clockwise)

Preceding gene: 66043437

Following gene: 66043442

Centisome position: 3.05

GC content: 56.19

Gene sequence:

>783_bases
GTGACAGACTTTCCAACTTCGTTGACCGCCCCCAAAAAGGGGTGTGAGGGCTGCACCGGCAGTCCGGAACTGGGCTTTTC
GTTTGATTATGCCTATCAACCCATCGTCAACCTGCACGACCAGTCGATCTTTGCCCATGAAGCGCTGGTCCGGGGCCCGA
ATGGCGAGGGCGCAATGTCAGTCCTGAGTCAGGTCACCGACCAGAACCGCTATCGCTTCGATCAGACCTGCCGGACCACG
GCCATCGCCGGCGCTGCGAAACTGGGCATGACCGAGCGTTTATCGATCAACTTTCTGCCAAATGCCGTGTATCGTCCCGA
GCTGTGTATCCGCAGCACGCTGGAAGCGGCCAAGCGGCATGACTTTCCCATCGAGCGGTTGATTTTCGAGACCATCGAAA
GCGAACATGTGTGCAGCAAAGGCCATTTGAGCAATATCCTGCGCGAGTACCGGGAGTTCGGCTTCATGACCGCTATCGAT
GACTTCGGCGCCGGGTATTCAGGCCTGACACTGCTGGCCGATTTTCAACCCGACCTGATCAAACTCGACATGAACCTGGT
ACGCGATGTTCATCGCAATCGCGCGCGTCAGGCGATTGTTCGCGCGGTTGTCACAATGTGTTCGGACTTGGGGATTAAAG
TCATCGCAGAAGGTATCGAACATATTGAAGAGCGGGATTTTCTGGCAGATTGCGGCATATCCCTGATGCAAGGCTATCTG
TTCGCCAAACCCGCATTCAAAGCCCTGGCTCAGGTAGCCCCCGAGGCCTGGCAGAAGTCTTGA

Upstream 100 bases:

>100_bases
TTGCTGATCTGTCGCTATCAAGAACATGAAGTAACCGATCCTTGCTTGTCAAAATCCGCTATTCGTCATTTTATGGCCCT
GACCTCACATGGAGTGCGAT

Downstream 100 bases:

>100_bases
GAAGTACGGAATCGCATTGAAGACATTCGATCATCTGACAGTCATCGGCCTGCGCGAGTGGGTGGCGCTCCCCGACCTGG
GGGTGGCAGGTCTGCGGGCA

Product: EAL

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTDFPTSLTAPKKGCEGCTGSPELGFSFDYAYQPIVNLHDQSIFAHEALVRGPNGEGAMSVLSQVTDQNRYRFDQTCRTT
AIAGAAKLGMTERLSINFLPNAVYRPELCIRSTLEAAKRHDFPIERLIFETIESEHVCSKGHLSNILREYREFGFMTAID
DFGAGYSGLTLLADFQPDLIKLDMNLVRDVHRNRARQAIVRAVVTMCSDLGIKVIAEGIEHIEERDFLADCGISLMQGYL
FAKPAFKALAQVAPEAWQKS

Sequences:

>Translated_260_residues
MTDFPTSLTAPKKGCEGCTGSPELGFSFDYAYQPIVNLHDQSIFAHEALVRGPNGEGAMSVLSQVTDQNRYRFDQTCRTT
AIAGAAKLGMTERLSINFLPNAVYRPELCIRSTLEAAKRHDFPIERLIFETIESEHVCSKGHLSNILREYREFGFMTAID
DFGAGYSGLTLLADFQPDLIKLDMNLVRDVHRNRARQAIVRAVVTMCSDLGIKVIAEGIEHIEERDFLADCGISLMQGYL
FAKPAFKALAQVAPEAWQKS
>Mature_259_residues
TDFPTSLTAPKKGCEGCTGSPELGFSFDYAYQPIVNLHDQSIFAHEALVRGPNGEGAMSVLSQVTDQNRYRFDQTCRTTA
IAGAAKLGMTERLSINFLPNAVYRPELCIRSTLEAAKRHDFPIERLIFETIESEHVCSKGHLSNILREYREFGFMTAIDD
FGAGYSGLTLLADFQPDLIKLDMNLVRDVHRNRARQAIVRAVVTMCSDLGIKVIAEGIEHIEERDFLADCGISLMQGYLF
AKPAFKALAQVAPEAWQKS

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=245, Percent_Identity=32.6530612244898, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1787541, Length=115, Percent_Identity=43.4782608695652, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510982, Length=230, Percent_Identity=26.9565217391304, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI87081845, Length=227, Percent_Identity=30.3964757709251, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790496, Length=225, Percent_Identity=26.6666666666667, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082096, Length=153, Percent_Identity=30.0653594771242, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788849, Length=153, Percent_Identity=31.3725490196078, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87081980, Length=227, Percent_Identity=25.9911894273128, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081743, Length=221, Percent_Identity=27.6018099547511, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788502, Length=230, Percent_Identity=23.9130434782609, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1787055, Length=241, Percent_Identity=29.045643153527, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786507, Length=136, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 28929; Mature: 28798

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFPTSLTAPKKGCEGCTGSPELGFSFDYAYQPIVNLHDQSIFAHEALVRGPNGEGAMS
CCCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VLSQVTDQNRYRFDQTCRTTAIAGAAKLGMTERLSINFLPNAVYRPELCIRSTLEAAKRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHC
DFPIERLIFETIESEHVCSKGHLSNILREYREFGFMTAIDDFGAGYSGLTLLADFQPDLI
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCHHH
KLDMNLVRDVHRNRARQAIVRAVVTMCSDLGIKVIAEGIEHIEERDFLADCGISLMQGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKPAFKALAQVAPEAWQKS
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDFPTSLTAPKKGCEGCTGSPELGFSFDYAYQPIVNLHDQSIFAHEALVRGPNGEGAMS
CCCCCCCCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
VLSQVTDQNRYRFDQTCRTTAIAGAAKLGMTERLSINFLPNAVYRPELCIRSTLEAAKRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHC
DFPIERLIFETIESEHVCSKGHLSNILREYREFGFMTAIDDFGAGYSGLTLLADFQPDLI
CCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCHHH
KLDMNLVRDVHRNRARQAIVRAVVTMCSDLGIKVIAEGIEHIEERDFLADCGISLMQGYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FAKPAFKALAQVAPEAWQKS
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]