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The map label for this gene is gsiD [H]

Identifier: 66043425

GI number: 66043425

Start: 168620

End: 169498

Strand: Reverse

Name: gsiD [H]

Synonym: Psyr_0155

Alternate gene names: 66043425

Gene position: 169498-168620 (Counterclockwise)

Preceding gene: 66043426

Following gene: 66043424

Centisome position: 2.78

GC content: 64.51

Gene sequence:

>879_bases
ATGACTCAATTCATTGCGAAATCGACGCCCGCAGCGCCGCAACCGGTGCTTCGCAAAAAAGCGTCCCTTCCGACCTCATG
GCTTGGCCTGCTGGGCGCTACGGTGTGCTTCATCTGGCTGATGGTCGCCCTGTTCGGCCCCTGGCTGGCACCGCATCCGG
TGGGCGAAGTGGTCTCCGACAACATGTTCGAAAACATGAGCGCAGCCTATCCCTTCGGTACGGACTACCTGGGCCGCGAC
ATGCTCAGCCGCGTGCTGGTCGGCGCGCGCTTCACCGTCGGCCTGGCATTGATTTCTGCCGTGCTGGCCAGCGGCATCGG
CACCCTGTGTGCGCTGCTGTCGGTGGTCGCGCCAAAATGGCTGGACGAAATCATCAGCCGCCTGATGGACGCGTTCATCT
CGATCCCCAGCAAAATGCTGGCGCTGATCATGGTCTCGGCGTTCGGCTCGTCCGAGGTGCTGCTGATCTGCACGGCGGTG
CTCAGCTACACCCCAGGCGCGTTTCGCATCGCGCGCAGTCTGGCGGTGAACATCGAGGCGCTGGAGTACGTGCAGGTAGC
GCGCACACGCGGCGAGGGTCGTATCTACATTGCCTGCCGGGAAATCCTGCCGAACATGCTCAACCCGGTCATGACCGACC
TGGGCCTGCGTTTCGGCTTCATCGTCCTGCTGCTCAGCGGCATGAGCTTTCTGGGGCTCGGTGTACAACCGCCTGACGCC
GACCTTGGCTCGCTGGTCCGCGAGAACATCGGCGGGCTCAGCCAGGGCGCGCCGGCCATCGTGATCCCGGCCCTGGCCAT
CGGCACCCTGACCATCGGCGTGAACCTGCTCATCGACCGGCTGGCCTCGCGGCGCGGTCGTCGTTCGGGAGGGCATTGA

Upstream 100 bases:

>100_bases
TGGTGCAGGGCTGCACGATGCTGTTCTGCTCCGCTTACCTTGGGTTGGTGCTGATGGCCGACCTGTGTGCAATTCTTTCC
AATCCGAGGCTGAGAACCCA

Downstream 100 bases:

>100_bases
GATGAGCCAGTTGATTCGTGTGCAAGACCTGCGCGTGGTTGCCGATGGCGACCACGGCGAGCTGGAGATCGTCAAAGGCG
TCAGCTTTGCGCTGGAAAAA

Product: binding-protein dependent transport system inner membrane protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTQFIAKSTPAAPQPVLRKKASLPTSWLGLLGATVCFIWLMVALFGPWLAPHPVGEVVSDNMFENMSAAYPFGTDYLGRD
MLSRVLVGARFTVGLALISAVLASGIGTLCALLSVVAPKWLDEIISRLMDAFISIPSKMLALIMVSAFGSSEVLLICTAV
LSYTPGAFRIARSLAVNIEALEYVQVARTRGEGRIYIACREILPNMLNPVMTDLGLRFGFIVLLLSGMSFLGLGVQPPDA
DLGSLVRENIGGLSQGAPAIVIPALAIGTLTIGVNLLIDRLASRRGRRSGGH

Sequences:

>Translated_292_residues
MTQFIAKSTPAAPQPVLRKKASLPTSWLGLLGATVCFIWLMVALFGPWLAPHPVGEVVSDNMFENMSAAYPFGTDYLGRD
MLSRVLVGARFTVGLALISAVLASGIGTLCALLSVVAPKWLDEIISRLMDAFISIPSKMLALIMVSAFGSSEVLLICTAV
LSYTPGAFRIARSLAVNIEALEYVQVARTRGEGRIYIACREILPNMLNPVMTDLGLRFGFIVLLLSGMSFLGLGVQPPDA
DLGSLVRENIGGLSQGAPAIVIPALAIGTLTIGVNLLIDRLASRRGRRSGGH
>Mature_291_residues
TQFIAKSTPAAPQPVLRKKASLPTSWLGLLGATVCFIWLMVALFGPWLAPHPVGEVVSDNMFENMSAAYPFGTDYLGRDM
LSRVLVGARFTVGLALISAVLASGIGTLCALLSVVAPKWLDEIISRLMDAFISIPSKMLALIMVSAFGSSEVLLICTAVL
SYTPGAFRIARSLAVNIEALEYVQVARTRGEGRIYIACREILPNMLNPVMTDLGLRFGFIVLLLSGMSFLGLGVQPPDAD
LGSLVRENIGGLSQGAPAIVIPALAIGTLTIGVNLLIDRLASRRGRRSGGH

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787054, Length=255, Percent_Identity=29.8039215686275, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787760, Length=260, Percent_Identity=32.3076923076923, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1789964, Length=252, Percent_Identity=30.5555555555556, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787549, Length=282, Percent_Identity=25.886524822695, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789889, Length=215, Percent_Identity=26.5116279069767, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787498, Length=202, Percent_Identity=30.6930693069307, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31068; Mature: 30937

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TQFIAKSTPAAPQPVLRKKASLPTSWLGLLGATVCFIWLMVALFGPWLAPHPVGEVVSD
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
NMFENMSAAYPFGTDYLGRDMLSRVLVGARFTVGLALISAVLASGIGTLCALLSVVAPKW
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDEIISRLMDAFISIPSKMLALIMVSAFGSSEVLLICTAVLSYTPGAFRIARSLAVNIEA
HHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LEYVQVARTRGEGRIYIACREILPNMLNPVMTDLGLRFGFIVLLLSGMSFLGLGVQPPDA
HHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
DLGSLVRENIGGLSQGAPAIVIPALAIGTLTIGVNLLIDRLASRRGRRSGGH
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]