| Definition | Pseudomonas syringae pv. syringae B728a, complete genome. |
|---|---|
| Accession | NC_007005 |
| Length | 6,093,698 |
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The map label for this gene is acnB [H]
Identifier: 161486734
GI number: 161486734
Start: 1958920
End: 1961520
Strand: Reverse
Name: acnB [H]
Synonym: Psyr_1726
Alternate gene names: 161486734
Gene position: 1961520-1958920 (Counterclockwise)
Preceding gene: 66044977
Following gene: 66044970
Centisome position: 32.19
GC content: 61.48
Gene sequence:
>2601_bases GTGCTTGAAGCCTACCGTAAACATATCGAAGAGCGTGCCGCCCAGGGCATCGTGCCCCAGCCGCTTAACGCCGAACAAAC TGCAGGCCTGGTCGAGCTGCTGAAGAATCCCCCGGCTGGCGAAGAAGCTTTCCTTGTCGATCTGATCACCAATCGCGTTC CGCCAGGCGTTGACGAAGCTGCCTACGTCAAGGCCGGTTTCCTGTCTGCCCTGGCCAAGGGCGAAGCCACTTCTCCCCTG ATCGACAAAAAACGCGCTACCGAACTGCTCGGCACCATGCAAGGTGGCTACAACATCGTCACCCTGGTTGAACTGCTCGA CGACGCCACCCTCGCGCCAGTCGCTGCCGAACAACTCAAGCACACCCTGCTGATGTTCGACGCCTTCCACGACGTGGCCG AAAAAGCCAAGAACGGCAATGCCCACGCCAAGGCTGTGCTGCAGTCCTGGGCTGACGGCGAGTGGTTCAAGAAGCGCCCG ACGCTGGCCGACAAGATCAGCCTGCGCGTCTTCAAGGTCACCGGCGAAACCAACACCGACGACCTGTCCCCTGCCCCTGA TGCCTGGTCGCGTCCGGACATCCCGCTGCACGCCCTGGCCATGCTGAAAATGGCCCGTGACGGCATCGTCCCTGACGTTC AGGGCGCCATCGGCCCGATGAAGCAGATCGAAGAAATGCGCGGTCAGGGCTTCCCGATTGCCTACGTCGGTGACGTGGTC GGTACCGGTTCCTCGCGTAAATCGGCAACCAACTCGGTACTCTGGTTCTTCGGCGACGACGTCCCTTACGTCCCGAACAA GCGTGCCGGCGGTTTCTGCTTCGGCAGCAAAATTGCTCCGATCTTCTACAACACCATGGAAGATGCCGGCGCTCTGCCAA TCGAATTCGATGTCTCGAACATCAACATGGGTGACGTGATCGACGTCTACCCCTACGCCGGCAAGGTCTGCAAGCACGAC AGCGACGAAGTCATCACCACCTTCGAAATGAAGACCCCGGTGCTGCTCGACGAAGTCCGCGCTGGCGGCCGTATTCCGCT GATCATCGGTCGTGGCCTGACCAGCAAGGCGCGCGCCGAACTGGGCCTGCCGGAGTTCGACCTGTTCAAGACCCCTGACC AGCCAGCCGAAAGCACCAAGGGTTACACCCTGGCGCAGAAGATGGTCGGCAAGGCATGCGGCGTAGCGGGCGTTCGTCCG GGCACCTACTGCGAACCGAAGATGACCACCGTCGGCTCCCAGGACACCACTGGCCCGATGACCCGCGACGAACTGAAAGA CCTGGCGTGCCTGGGCTTCTCGACCGATCTGGTCATGCAGTCGTTCTGCCACACCGCGGCCTATCCGAAGCCGATCGACG TCAAGACGCACCACACGCTGCCTGACTTCATCATGACCCGCGGTGGCGTTTCCCTGCGTCCGGGCGACGGCATCATCCAC AGCTGGCTGAACCGCATGCTGCTGCCGGACACCGTCGGCACCGGTGGCGACTCGCACACCCGCTTCCCGATCGGTATCTC CTTCCCGGCCGGTTCCGGCCTGGTGGCGTTCGCTGCTGCAACGGGCGTCATGCCGCTGGACATGCCTGAATCGATCCTGG TTCGCTTCAAGGGCAAGATGCAACCTGGCATCACCCTGCGCGACCTGGTTCACGCCATTCCTTACTACGCGATTCAGGCT GGCCTGCTGACCGTAGAGAAGAAAGGCAAGAAGAACGCATTCTCCGGTCGCATCCTGGAGATCGAAGGCCTCGACAACCT GAGCATCGAACAGGCTTTCGAGCTGTCCGACGCCTCTGCCGAGCGTTCGGCTGCCGGTTGCACCATCAAGCTGGCCAAAG AGCCGATCATCGAATACCTGAATTCGAACATCACCCTGCTGCGCTGGATGATCGAACAGGGTTACGGTGATCCTCGCACC CTGGAGCGTCGTGCTCAGGCGATGGAAGCCTGGGTTGCCAACCCGGAGCTGCTGGAAGCCGACAAGGACGCCGAGTACGC CGAAATCATCGAAATCGATCTGGCCGACGTCAAGGAGCCTGTGCTCTGCGCGCCGAACGACCCGGACGATGCCCGCCTGC TGTCTTCGGTACAGGGCGAGAAGATCGACGAAGTGTTCATCGGTTCGTGCATGACCAACATCGGCCACTTCCGCGCTGCG GGCAAGTTGCTGGATCAGGTCAAGGGCCAGCTGCCAACGCGTCTGTGGCTGTCGCCACCGACCAAGATGGATGCTCACCA ACTGACCGAAGAAGGCTACTACGGCATCTATGGCAAGGCTGGCGCACGCATGGAAATGCCGGGCTGCTCGCTGTGCATGG GTAACCAGGCACGCGTTGAACCGAACGCGACCGTGGTGTCGACCTCGACCCGTAACTTCCCGAACCGTCTGGGTGACGGC GCGAACGTCTACCTGGCCTCGGCCGAGCTGGCGTCCGTTGCCTCGATTCTGGGTCGTCTGCCGACGGTCGAAGAGTACAT GGGCTACGCCAATCAGATCGACACCATGGCAGCGGACGTGTACCGCTACCTGAGCTTCGATCAGATCGCCGAGTTCCGTG AAGCCGCAGCGAACGCCAACATCCCGGCCGTTCAAGTGTAA
Upstream 100 bases:
>100_bases TACCCTGCCAACAAGGCTCAATAAGCCTTGCGTGGGTACGGGCGATTTCTGGATGAGCGTCCCGGACACCCATTTGGGAC CACTGATGAGGGTTAATACT
Downstream 100 bases:
>100_bases GTACGAAAGCAGGCATCAGAAGAACCCCATCCTCGTGATGGGGTTTTTTTGCTTTCGAGCATGCCCGTACATATATTTTT CACATTCACATGGCTATAGT
Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase
Products: NA
Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]
Number of amino acids: Translated: 866; Mature: 866
Protein sequence:
>866_residues MLEAYRKHIEERAAQGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEAAYVKAGFLSALAKGEATSPL IDKKRATELLGTMQGGYNIVTLVELLDDATLAPVAAEQLKHTLLMFDAFHDVAEKAKNGNAHAKAVLQSWADGEWFKKRP TLADKISLRVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDVQGAIGPMKQIEEMRGQGFPIAYVGDVV GTGSSRKSATNSVLWFFGDDVPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSNINMGDVIDVYPYAGKVCKHD SDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTSKARAELGLPEFDLFKTPDQPAESTKGYTLAQKMVGKACGVAGVRP GTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSTDLVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIH SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKMQPGITLRDLVHAIPYYAIQA GLLTVEKKGKKNAFSGRILEIEGLDNLSIEQAFELSDASAERSAAGCTIKLAKEPIIEYLNSNITLLRWMIEQGYGDPRT LERRAQAMEAWVANPELLEADKDAEYAEIIEIDLADVKEPVLCAPNDPDDARLLSSVQGEKIDEVFIGSCMTNIGHFRAA GKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVEPNATVVSTSTRNFPNRLGDG ANVYLASAELASVASILGRLPTVEEYMGYANQIDTMAADVYRYLSFDQIAEFREAAANANIPAVQV
Sequences:
>Translated_866_residues MLEAYRKHIEERAAQGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEAAYVKAGFLSALAKGEATSPL IDKKRATELLGTMQGGYNIVTLVELLDDATLAPVAAEQLKHTLLMFDAFHDVAEKAKNGNAHAKAVLQSWADGEWFKKRP TLADKISLRVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDVQGAIGPMKQIEEMRGQGFPIAYVGDVV GTGSSRKSATNSVLWFFGDDVPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSNINMGDVIDVYPYAGKVCKHD SDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTSKARAELGLPEFDLFKTPDQPAESTKGYTLAQKMVGKACGVAGVRP GTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSTDLVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIH SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKMQPGITLRDLVHAIPYYAIQA GLLTVEKKGKKNAFSGRILEIEGLDNLSIEQAFELSDASAERSAAGCTIKLAKEPIIEYLNSNITLLRWMIEQGYGDPRT LERRAQAMEAWVANPELLEADKDAEYAEIIEIDLADVKEPVLCAPNDPDDARLLSSVQGEKIDEVFIGSCMTNIGHFRAA GKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVEPNATVVSTSTRNFPNRLGDG ANVYLASAELASVASILGRLPTVEEYMGYANQIDTMAADVYRYLSFDQIAEFREAAANANIPAVQV >Mature_866_residues MLEAYRKHIEERAAQGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEAAYVKAGFLSALAKGEATSPL IDKKRATELLGTMQGGYNIVTLVELLDDATLAPVAAEQLKHTLLMFDAFHDVAEKAKNGNAHAKAVLQSWADGEWFKKRP TLADKISLRVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKMARDGIVPDVQGAIGPMKQIEEMRGQGFPIAYVGDVV GTGSSRKSATNSVLWFFGDDVPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSNINMGDVIDVYPYAGKVCKHD SDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTSKARAELGLPEFDLFKTPDQPAESTKGYTLAQKMVGKACGVAGVRP GTYCEPKMTTVGSQDTTGPMTRDELKDLACLGFSTDLVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIH SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKMQPGITLRDLVHAIPYYAIQA GLLTVEKKGKKNAFSGRILEIEGLDNLSIEQAFELSDASAERSAAGCTIKLAKEPIIEYLNSNITLLRWMIEQGYGDPRT LERRAQAMEAWVANPELLEADKDAEYAEIIEIDLADVKEPVLCAPNDPDDARLLSSVQGEKIDEVFIGSCMTNIGHFRAA GKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVEPNATVVSTSTRNFPNRLGDG ANVYLASAELASVASILGRLPTVEEYMGYANQIDTMAADVYRYLSFDQIAEFREAAANANIPAVQV
Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]
COG id: COG1049
COG function: function code C; Aconitase B
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aconitase/IPM isomerase family [H]
Homologues:
Organism=Escherichia coli, GI2367097, Length=856, Percent_Identity=78.9719626168224, Blast_Score=1398, Evalue=0.0, Organism=Escherichia coli, GI1786259, Length=390, Percent_Identity=24.8717948717949, Blast_Score=77, Evalue=4e-15, Organism=Saccharomyces cerevisiae, GI6323335, Length=382, Percent_Identity=26.9633507853403, Blast_Score=76, Evalue=2e-14, Organism=Saccharomyces cerevisiae, GI6322261, Length=400, Percent_Identity=26.75, Blast_Score=71, Evalue=8e-13, Organism=Drosophila melanogaster, GI161076999, Length=388, Percent_Identity=25.7731958762887, Blast_Score=70, Evalue=5e-12, Organism=Drosophila melanogaster, GI281365315, Length=388, Percent_Identity=26.0309278350515, Blast_Score=70, Evalue=7e-12, Organism=Drosophila melanogaster, GI17864292, Length=388, Percent_Identity=26.0309278350515, Blast_Score=70, Evalue=7e-12,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR015931 - InterPro: IPR015937 - InterPro: IPR001030 - InterPro: IPR015928 - InterPro: IPR015932 - InterPro: IPR018136 - InterPro: IPR004406 - InterPro: IPR015930 - InterPro: IPR015933 - InterPro: IPR015929 [H]
Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]
EC number: =4.2.1.3; =4.2.1.99 [H]
Molecular weight: Translated: 93793; Mature: 93793
Theoretical pI: Translated: 4.91; Mature: 4.91
Prosite motif: PS00450 ACONITASE_1
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.3 %Cys (Translated Protein) 3.2 %Met (Translated Protein) 4.5 %Cys+Met (Translated Protein) 1.3 %Cys (Mature Protein) 3.2 %Met (Mature Protein) 4.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MLEAYRKHIEERAAQGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEA CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHH AYVKAGFLSALAKGEATSPLIDKKRATELLGTMQGGYNIVTLVELLDDATLAPVAAEQLK HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHH HTLLMFDAFHDVAEKAKNGNAHAKAVLQSWADGEWFKKRPTLADKISLRVFKVTGETNTD HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCHHEEEEEEEEEECCCCCC DLSPAPDAWSRPDIPLHALAMLKMARDGIVPDVQGAIGPMKQIEEMRGQGFPIAYVGDVV CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCCCCEEEECCHH GTGSSRKSATNSVLWFFGDDVPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSN CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCC INMGDVIDVYPYAGKVCKHDSDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTSKARAE CCCCCEEEECCCCCHHHCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEECCCCHHHHHH LGLPEFDLFKTPDQPAESTKGYTLAQKMVGKACGVAGVRPGTYCEPKMTTVGSQDTTGPM CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCC TRDELKDLACLGFSTDLVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIH CHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCHHH SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKM HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHCCCCC QPGITLRDLVHAIPYYAIQAGLLTVEKKGKKNAFSGRILEIEGLDNLSIEQAFELSDASA CCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCH ERSAAGCTIKLAKEPIIEYLNSNITLLRWMIEQGYGDPRTLERRAQAMEAWVANPELLEA HHCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCC DKDAEYAEIIEIDLADVKEPVLCAPNDPDDARLLSSVQGEKIDEVFIGSCMTNIGHFRAA CCCCCCHHHEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH GKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVE HHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCEEEECCCCCEEECCCCCEEECCCCEEC PNATVVSTSTRNFPNRLGDGANVYLASAELASVASILGRLPTVEEYMGYANQIDTMAADV CCCEEEECCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHH YRYLSFDQIAEFREAAANANIPAVQV HHHHCHHHHHHHHHHHCCCCCCEEEC >Mature Secondary Structure MLEAYRKHIEERAAQGIVPQPLNAEQTAGLVELLKNPPAGEEAFLVDLITNRVPPGVDEA CCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCHH AYVKAGFLSALAKGEATSPLIDKKRATELLGTMQGGYNIVTLVELLDDATLAPVAAEQLK HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHH HTLLMFDAFHDVAEKAKNGNAHAKAVLQSWADGEWFKKRPTLADKISLRVFKVTGETNTD HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCCHHEEEEEEEEEECCCCCC DLSPAPDAWSRPDIPLHALAMLKMARDGIVPDVQGAIGPMKQIEEMRGQGFPIAYVGDVV CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCCCCEEEECCHH GTGSSRKSATNSVLWFFGDDVPYVPNKRAGGFCFGSKIAPIFYNTMEDAGALPIEFDVSN CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHHCCCCEEEEECCC INMGDVIDVYPYAGKVCKHDSDEVITTFEMKTPVLLDEVRAGGRIPLIIGRGLTSKARAE CCCCCEEEECCCCCHHHCCCCCCEEEEEECCCCHHHHHHCCCCCEEEEEECCCCHHHHHH LGLPEFDLFKTPDQPAESTKGYTLAQKMVGKACGVAGVRPGTYCEPKMTTVGSQDTTGPM CCCCCCCCCCCCCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCC TRDELKDLACLGFSTDLVMQSFCHTAAYPKPIDVKTHHTLPDFIMTRGGVSLRPGDGIIH CHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCEECCCCCHHH SWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESILVRFKGKM HHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEEHHHHCCCCCCCCCHHHHHHCCCCC QPGITLRDLVHAIPYYAIQAGLLTVEKKGKKNAFSGRILEIEGLDNLSIEQAFELSDASA CCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCH ERSAAGCTIKLAKEPIIEYLNSNITLLRWMIEQGYGDPRTLERRAQAMEAWVANPELLEA HHCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHCCC DKDAEYAEIIEIDLADVKEPVLCAPNDPDDARLLSSVQGEKIDEVFIGSCMTNIGHFRAA CCCCCCHHHEEEEHHHCCCCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH GKLLDQVKGQLPTRLWLSPPTKMDAHQLTEEGYYGIYGKAGARMEMPGCSLCMGNQARVE HHHHHHHHCCCCCEEECCCCCCCCHHHHHHCCCEEEECCCCCEEECCCCCEEECCCCEEC PNATVVSTSTRNFPNRLGDGANVYLASAELASVASILGRLPTVEEYMGYANQIDTMAADV CCCEEEECCCCCCHHHCCCCCEEEEEHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHH YRYLSFDQIAEFREAAANANIPAVQV HHHHCHHHHHHHHHHHCCCCCCEEEC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]