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The map label for this gene is pcnA [H]

Identifier: 62391931

GI number: 62391931

Start: 3263928

End: 3265412

Strand: Reverse

Name: pcnA [H]

Synonym: cg3415

Alternate gene names: 62391931

Gene position: 3265412-3263928 (Counterclockwise)

Preceding gene: 62391932

Following gene: 62391930

Centisome position: 99.47

GC content: 53.0

Gene sequence:

>1485_bases
GTGAATTCCCAGGATGCTCAGAACCAAGTTCCCCCACAGAAAGTGGACCAGGAAGTAGACCGCCTTGCTTTAATGGCGCG
CGCTCACCAGGCAATTGGTGAGCTTTCAGACATCCTTGTGCCCCTAGCGGCAGCATTTTCCAGCAAGGGACACTCCCTAT
ATTTGGTGGGCGGTTCCGTGAGGGATGCTTTCTTAGGTGAGTTGGGGCATGACCTTGATTTCACCACGTCGGCACGCCCT
GAGGAGACAAAGGCGATCCTGGATGATTACGCCGATGTGGTGTGGGATACCGGAATTGCGTTTGGCACCCTTTCTGCAGA
AAAGCATGGTCAGCAGATCGAAATCACGACATTCCGTTCTGATCTCTATGACGGAAACTCCCGCAACCCTGAAGTGACCT
TCGGTGACACCTTGGAAGGCGATCTGATTAGGCGCGATTTCAAGGTCAACGCCATGGCTGTGGAGATCCAAGCCGATGGT
GAATTAACTTTCCACGATCCAGTGGGTGGCTTGGAAGATCTACTTACCCACACTTTGGATACCCCAGCTACTCCGGAACA
ATCATTCAATGATGATCCTCTGCGAATGCTGCGCGCTGCCCGCTTTGTTTCCCAACTGAATTTCACCCTTGCACCACGAG
TTATTACCGCCATGACTGAAATGGCGCAGCAGATTACTCGGATCACTGTGGAACGCATGCAGGTTGAGTTGGACAAAATG
ATCCTTGGCAAAAACCCTGAGGCTGGCATTGACCTCATGGTGGAGTCCGGTATCGCGCAGATTATTTATCCGGAGATCCC
TGCGATGCAGATGACTCAAGATGAACACATGCAGCACAAGGATGTGTACGCGCACTCGTTGCAGGTGATGCGCCAAGCGA
TTGATCAGGAAGAAGATGGCCCTGACCTGGTGCTTCGTTGGGCTGCTCTGCTTCATGATTGCGGTAAGCCAGATACTCGT
GACTTCAATGAGGAAGGCCGCGTGAGCTTCCATCAGCATGAGGTAGTTGGCGCCAAGCTGGTGAGGCGACGGATGCGCAA
GCTGAAGTACTCAAAGCAAATGGTCGGCGATGTCGGGCAGTTGGTGTTCCTGCACATGCGTTTCCATGGTTTCAGCGAAG
GCCAGTGGACGGATTCTGCAGTGCGTAGGTATGCCGCAGATGCGGGTGAATTGCTGCCACGTTTGCACAAGTTAGTGCGT
GCTGATTGCACCACAAGAAATAAAAGGAAGGCAGCACGACTGCAAGCCACCTACGATCATTTGGAAGAGCGCATCGCGGA
GATCGCCGCAAAGGAAGATCTTGCCAGGGTGCGCCCAGATTTGGATGGCAATGAGATCATGGAGATTCTGAACATCCAAG
CCGGACCTGAAGTGGGTAAGGCGTGGGCGTTTTTGAAGGAGCTTCGTTTGGAGCGCGGTCCTTTGGATCGTGAAGTTGCC
ATCGCAGAGCTGAAGAGCTGGTGGGAAGGAGAAAACAATGAGTGA

Upstream 100 bases:

>100_bases
GGCCTTAAACGGGCTCCTACGGTGCAAAGTGCGAAAACTTATTCTAATTTCACATTTAGAGCAGTTTTTGCCCACACTTC
TACTGGGTAGACTCAGATTC

Downstream 100 bases:

>100_bases
TTTTTATGCCGACAGGTTGTTTAACGCGATGGAGCGCAATGAGGTAGCTCCAGGCATGTTGTTGGTGGCTGCGCCCGATA
TGGCGTCGGAGGATTTTGAG

Product: polyA polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNSQDAQNQVPPQKVDQEVDRLALMARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARP
EETKAILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAVEIQADG
ELTFHDPVGGLEDLLTHTLDTPATPEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKM
ILGKNPEAGIDLMVESGIAQIIYPEIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTR
DFNEEGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVR
ADCTTRNKRKAARLQATYDHLEERIAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVA
IAELKSWWEGENNE

Sequences:

>Translated_494_residues
MNSQDAQNQVPPQKVDQEVDRLALMARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARP
EETKAILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAVEIQADG
ELTFHDPVGGLEDLLTHTLDTPATPEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKM
ILGKNPEAGIDLMVESGIAQIIYPEIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTR
DFNEEGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVR
ADCTTRNKRKAARLQATYDHLEERIAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVA
IAELKSWWEGENNE
>Mature_494_residues
MNSQDAQNQVPPQKVDQEVDRLALMARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSVRDAFLGELGHDLDFTTSARP
EETKAILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRSDLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAVEIQADG
ELTFHDPVGGLEDLLTHTLDTPATPEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKM
ILGKNPEAGIDLMVESGIAQIIYPEIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDGPDLVLRWAALLHDCGKPDTR
DFNEEGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQLVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVR
ADCTTRNKRKAARLQATYDHLEERIAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVA
IAELKSWWEGENNE

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=228, Percent_Identity=31.140350877193, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1789436, Length=334, Percent_Identity=30.5389221556886, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87081691, Length=260, Percent_Identity=26.9230769230769, Blast_Score=96, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71995920, Length=221, Percent_Identity=25.7918552036199, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=233, Percent_Identity=31.3304721030043, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281360112, Length=294, Percent_Identity=26.8707482993197, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357337, Length=294, Percent_Identity=26.8707482993197, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 55641; Mature: 55641

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSQDAQNQVPPQKVDQEVDRLALMARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
RDAFLGELGHDLDFTTSARPEETKAILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCEEEEEEEHH
DLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAVEIQADGELTFHDPVGGLEDLLTHTLD
HHCCCCCCCCCEEECCCCCCHHHHHCEEEEEEEEEEEECCEEEECCCCCCHHHHHHHHCC
TPATPEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKM
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGKNPEAGIDLMVESGIAQIIYPEIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDG
HHCCCCCCCHHHHHHCCCHHEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDLVLRWAALLHDCGKPDTRDFNEEGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVRADCTTRNKRKAARLQATYDH
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEERIAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
IAELKSWWEGENNE
HHHHHHHHCCCCCC
>Mature Secondary Structure
MNSQDAQNQVPPQKVDQEVDRLALMARAHQAIGELSDILVPLAAAFSSKGHSLYLVGGSV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCH
RDAFLGELGHDLDFTTSARPEETKAILDDYADVVWDTGIAFGTLSAEKHGQQIEITTFRS
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCHHHCCCEEEEEEEHH
DLYDGNSRNPEVTFGDTLEGDLIRRDFKVNAMAVEIQADGELTFHDPVGGLEDLLTHTLD
HHCCCCCCCCCEEECCCCCCHHHHHCEEEEEEEEEEEECCEEEECCCCCCHHHHHHHHCC
TPATPEQSFNDDPLRMLRAARFVSQLNFTLAPRVITAMTEMAQQITRITVERMQVELDKM
CCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILGKNPEAGIDLMVESGIAQIIYPEIPAMQMTQDEHMQHKDVYAHSLQVMRQAIDQEEDG
HHCCCCCCCHHHHHHCCCHHEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PDLVLRWAALLHDCGKPDTRDFNEEGRVSFHQHEVVGAKLVRRRMRKLKYSKQMVGDVGQ
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVFLHMRFHGFSEGQWTDSAVRRYAADAGELLPRLHKLVRADCTTRNKRKAARLQATYDH
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LEERIAEIAAKEDLARVRPDLDGNEIMEILNIQAGPEVGKAWAFLKELRLERGPLDREVA
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHH
IAELKSWWEGENNE
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA