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The map label for this gene is thiD2 [H]

Identifier: 62391925

GI number: 62391925

Start: 3260698

End: 3261498

Strand: Reverse

Name: thiD2 [H]

Synonym: cg3409

Alternate gene names: 62391925

Gene position: 3261498-3260698 (Counterclockwise)

Preceding gene: 62391928

Following gene: 62391924

Centisome position: 99.35

GC content: 55.93

Gene sequence:

>801_bases
GTGGTTAATTACGCATTCGTTATTGCAGGTTCAGAAGCTACCGGCGGCGCCGGCATCCAGGTTGATTTGAAGACCTTCCA
GCATTTAGATGTTTATGGCATTGGTGCCATCACATGCTTGGTGGCCTTTGATCCTAAAGACAACTGGAATCACCGTTTTG
TCCCGGTTGATGCGCAGGTTATCGCTAATCAGATTGAGGCCGCCACCGCAGCGCACGATCTTGATGTGGTGAAGATCGGT
ATGTTGGGTACTCCTGCAACGATCGATACTGTGGCAACCGCTTTGGAGGAAAACAGCTTCAAGCACGTTGTCCTAGACCC
GGTACTGATCTGCAAGGGCCAGGAGCCCGGCGCGGCACTTGATACTGACACTGCCCTTCGCGCGAAGGTGCTGCCACAGG
CAACCGTGGTTACTCCAAACAACTTCGAGGCCACCACCCTGTCTGGCCTAGACAAGCTGGAGACCATCGACGACCTGAAG
GAAGCAGCCCGCCTCATTCATGAGCAAGGACCTCAGTACGTCGTTGTTAAGGGTGGCATCGACTTCCCAGGCGACAACGC
TGTGGACGTACTTTTCGACGGCACCGACTACCACGTGTTCTCTGAACCAAAGATCGGCGACGAGCGCGTCTCCGGCGCTG
GCTGTACCTTCGCAGCTGTCATCACCGCAGAGCTGGCCAAGGGCAACTCTGCCGTTGATGCAGTGACCACCGCTAAGCGC
GTAGTCACCCGCGCAGTGAAGGACGCTGTCGCATCCAACGCACCGTTTACCTCTGTGTGGTTGGCGGAAGACAACAAGTA
G

Upstream 100 bases:

>100_bases
GTGCGCACGATGATCGTTTTCGACATGCTCTTTAGCCTACGGATGAAATTTTTCAGACGGTAATGTCGGAATCCTCCACC
TGAGGGGCTAGGTTTGAAGT

Downstream 100 bases:

>100_bases
AAATCTTAAACAAGCTCCCTTTTTGCGCGTTAAAAGCCGCATAAAGGGAGCTTTGCTATTGGATTTCAAACAGTCGTTGA
AGGTGCAAAGCGTTGTTACA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIG
MLGTPATIDTVATALEENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLK
EAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSAVDAVTTAKR
VVTRAVKDAVASNAPFTSVWLAEDNK

Sequences:

>Translated_266_residues
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIG
MLGTPATIDTVATALEENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLK
EAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSAVDAVTTAKR
VVTRAVKDAVASNAPFTSVWLAEDNK
>Mature_266_residues
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQVIANQIEAATAAHDLDVVKIG
MLGTPATIDTVATALEENSFKHVVLDPVLICKGQEPGAALDTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLK
EAARLIHEQGPQYVVVKGGIDFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSAVDAVTTAKR
VVTRAVKDAVASNAPFTSVWLAEDNK

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates. Can also use hydroxymethylpyrimidine (HMP) as substrate [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=245, Percent_Identity=31.8367346938775, Blast_Score=122, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6325378, Length=254, Percent_Identity=28.740157480315, Blast_Score=82, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6324997, Length=273, Percent_Identity=30.4029304029304, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324517, Length=275, Percent_Identity=30.5454545454545, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.35 [H]

Molecular weight: Translated: 28061; Mature: 28061

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQV
CCEEEEEEECCCCCCCCCEEEEEHHHCCCCEEECCEEEEEEEECCCCCCCEEEEECHHHH
IANQIEAATAAHDLDVVKIGMLGTPATIDTVATALEENSFKHVVLDPVLICKGQEPGAAL
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECEEEEEECCCCCCCC
DTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLKEAARLIHEQGPQYVVVKGGI
CCCHHHHHEECCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
DFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSAVDAVTTAKR
CCCCCCEEEEEECCCCEEEECCCCCCHHHCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHH
VVTRAVKDAVASNAPFTSVWLAEDNK
HHHHHHHHHHHCCCCCEEEEEECCCC
>Mature Secondary Structure
MVNYAFVIAGSEATGGAGIQVDLKTFQHLDVYGIGAITCLVAFDPKDNWNHRFVPVDAQV
CCEEEEEEECCCCCCCCCEEEEEHHHCCCCEEECCEEEEEEEECCCCCCCEEEEECHHHH
IANQIEAATAAHDLDVVKIGMLGTPATIDTVATALEENSFKHVVLDPVLICKGQEPGAAL
HHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECEEEEEECCCCCCCC
DTDTALRAKVLPQATVVTPNNFEATTLSGLDKLETIDDLKEAARLIHEQGPQYVVVKGGI
CCCHHHHHEECCCEEEECCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
DFPGDNAVDVLFDGTDYHVFSEPKIGDERVSGAGCTFAAVITAELAKGNSAVDAVTTAKR
CCCCCCEEEEEECCCCEEEECCCCCCHHHCCCCCCEEEEHHHHHHHCCCCHHHHHHHHHH
VVTRAVKDAVASNAPFTSVWLAEDNK
HHHHHHHHHHHCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]