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The map label for this gene is pyk [H]

Identifier: 62391746

GI number: 62391746

Start: 3083768

End: 3085627

Strand: Reverse

Name: pyk [H]

Synonym: cg3218

Alternate gene names: 62391746

Gene position: 3085627-3083768 (Counterclockwise)

Preceding gene: 62391747

Following gene: 62391744

Centisome position: 94.0

GC content: 58.28

Gene sequence:

>1860_bases
ATGAATGAGTTTGACCAGGACATTCTCCAGGAGATCAAGACTGAACTCGACGAGTTAATTCTAGAACTTGATGAGGTGAC
ACAAACTCACAGCGAGGCCATCGGGCAGGTCTCCCCAACCCATTACGTTGGTGCCCGCAACCTCATGCATTACGCGCATC
TTCGCACCAAAGACCTCCGTGGCCTGCAGCAACGCCTCTCCTCTGTGGGAGCTACCCGCTTGACTACCACCGAACCAGCA
GTGCAGGCCCGCCTCAAGGCCGCCCGCAATGTTATCGGAGCTTTCGCAGGTGAAGGCCCACTTTATCCACCCTCAGATGT
CGTCGATGCCTTCGAAGATGCCGATGAGATTCTCGACGAGCACGCCGAAATTCTCCTTGGCGAACCCCTACCGGATACTC
CATCCTGCATCATGGTCACCCTGCCCACCGAAGCCGCCACCGACATTGAACTTGTCCGTGGCTTCGCCAAAAGCGGCATG
AATCTAGCTCGCATCAACTGTGCACACGACGATGAAACCGTCTGGAAGCAGATGATCGACAACGTCCACACCGTTGCAGA
AGAAGTTGGCCGGGAAATCCGCGTCAGCATGGACCTCGCCGGACCAAAAGTACGCACCGGCGAAATCGCCCCAGGCGCAG
AAGTAGGTCGCGCACGAGTAACCCGCGACGAAACCGGAAAAGTACTGACGCCCGCAAAACTGTGGATCACCGCCCACGGC
TCCGAACCAGTCCCAGCCCCCGAAAGCCTGCCCGGTCGCCCCGCTCTGCCGATTGAAGTCACCCCAGAATGGTTCGACAA
ACTAGAAATCGGCAGCGTCATCAACGTCCCAGACACCCGCGGATCCCGCCGAGCATTCACCGTGACCAGGGTTTTTGATG
GCGCGGTCCTCGCCGAAGGCCCACAAAAAGCCTACATCTCCAACGGCACCCTCCTGGAACACAACTACGACCGCTCCCGG
GTCTACGGCATCCCCGCCGTAGTTCAGCGCATCAACCTCAAAGTCGGCGACCGCCTCATCCTTACCGACGAAGAACTCAC
CTACGATCCATCCCTCGGATCCGGCCGCACACCACGCATCAGCTGCACCCTTCCACAAGCAGTCGATGCAATTAAAGTCG
GGCACCGCGTGCTTTTCGACGACGGAGCCATCGCCGCAGTCTGCATCGACAAGACCTCCACTGCCGACGGCCACAACGAC
GTAGAATTGGAAGTCACCCACGCCCGCCCACAAGGCGTAAACCTGGCCGCATACAAGGGAATCAACCTCCCAGACTCCGA
ACTTCCACTCCCAAGCCTCACTGAAGAAGACCTCCAACACCTGCGCTTTGTCGTCAAATACGCCGACATCGCAGCCATCT
CCTTCATCCGAAACGTCGCCGACGTGGAATACCTCCTCCAAGCACTCGCCGACATCGGAGATCCAGTAGCCGTCGAACGC
CTTGGCCTCGTCCTTAAAATCGAGACCATCCCAGGCTACGAAGGCCTCGCCCAAATCCTCCTGACCGGCATGCGCCACGA
AAACTTCGGCATCATGATCGCCCGCGGAGACCTCGCCGTCGAACTCGGCTTCGACCGCATGGCAGAAGTCCCCCAACTGA
TCATGGCCCTTGCCGAAGCCGCCCACGTCCCAACCATCTTGGCCACCCAAGTCCTGGAAAACATGGCCAAAAACGGACTC
CCATCTCGCGCAGAAATCACCGACGCAGCAATGGCACTTCGCGCTGAATGCGTCATGCTGAACAAGGGACCACACATCAA
CGACGCCATCAAGGTCCTCACCGAAATGAGCCGCAAACTTGGTGCATCCCAACGAAAGAGTAGGCTGCTGCTGCGCAAGG
TGAAGAGCTGGGAAGAGTAA

Upstream 100 bases:

>100_bases
AAGTGTTTCATTGGAACACTTGCGCTGCCAACTTTTTGGTTTACGGGCACAATGAAACTGTTGGATGGAATTTAGAGTGT
TTGTAGCTTAAGGAGCTCAA

Downstream 100 bases:

>100_bases
CTCACAAAGGCGATTGGCGTTAACTTCGAGATCTTAACAGCGATTTCACGTTTACAGTTTCGAGCGCTCAATTTCTCAGG
TTAGATACCAGTCAGTTTTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 619; Mature: 619

Protein sequence:

>619_residues
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLRGLQQRLSSVGATRLTTTEPA
VQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDEHAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGM
NLARINCAHDDETVWKQMIDNVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDETGKVLTPAKLWITAHG
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEGPQKAYISNGTLLEHNYDRSR
VYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRISCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKTSTADGHND
VELEVTHARPQGVNLAAYKGINLPDSELPLPSLTEEDLQHLRFVVKYADIAAISFIRNVADVEYLLQALADIGDPVAVER
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEAAHVPTILATQVLENMAKNGL
PSRAEITDAAMALRAECVMLNKGPHINDAIKVLTEMSRKLGASQRKSRLLLRKVKSWEE

Sequences:

>Translated_619_residues
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLRGLQQRLSSVGATRLTTTEPA
VQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDEHAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGM
NLARINCAHDDETVWKQMIDNVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDETGKVLTPAKLWITAHG
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEGPQKAYISNGTLLEHNYDRSR
VYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRISCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKTSTADGHND
VELEVTHARPQGVNLAAYKGINLPDSELPLPSLTEEDLQHLRFVVKYADIAAISFIRNVADVEYLLQALADIGDPVAVER
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEAAHVPTILATQVLENMAKNGL
PSRAEITDAAMALRAECVMLNKGPHINDAIKVLTEMSRKLGASQRKSRLLLRKVKSWEE
>Mature_619_residues
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLRGLQQRLSSVGATRLTTTEPA
VQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDEHAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGM
NLARINCAHDDETVWKQMIDNVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDETGKVLTPAKLWITAHG
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEGPQKAYISNGTLLEHNYDRSR
VYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRISCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKTSTADGHND
VELEVTHARPQGVNLAAYKGINLPDSELPLPSLTEEDLQHLRFVVKYADIAAISFIRNVADVEYLLQALADIGDPVAVER
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEAAHVPTILATQVLENMAKNGL
PSRAEITDAAMALRAECVMLNKGPHINDAIKVLTEMSRKLGASQRKSRLLLRKVKSWEE

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI10835121, Length=273, Percent_Identity=31.5018315018315, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI32967597, Length=273, Percent_Identity=31.5018315018315, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI33286422, Length=253, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33286420, Length=253, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI33286418, Length=240, Percent_Identity=31.25, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI310128732, Length=180, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310128730, Length=180, Percent_Identity=28.8888888888889, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1787965, Length=276, Percent_Identity=30.0724637681159, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1788160, Length=258, Percent_Identity=26.7441860465116, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI71984406, Length=273, Percent_Identity=29.6703296703297, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17506831, Length=273, Percent_Identity=29.6703296703297, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71984413, Length=273, Percent_Identity=29.6703296703297, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17506829, Length=273, Percent_Identity=29.6703296703297, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17544584, Length=243, Percent_Identity=30.0411522633745, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6319279, Length=190, Percent_Identity=34.2105263157895, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324923, Length=201, Percent_Identity=32.8358208955224, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI28571814, Length=254, Percent_Identity=33.0708661417323, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648964, Length=254, Percent_Identity=33.0708661417323, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648966, Length=279, Percent_Identity=32.258064516129, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24581235, Length=252, Percent_Identity=24.6031746031746, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 67573; Mature: 67573

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLQQRLSSVGATRLTTTEPAVQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDE
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
HAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGMNLARINCAHDDETVWKQMID
HHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
NVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDETGKVLTPAKLWITAHG
HHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCHHCCCCCCCCCCCCEECCEEEEEEECC
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEG
CCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCEECCCCCCCCCEEEEEEEECCCCCCCCC
PQKAYISNGTLLEHNYDRSRVYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRI
CCCEEECCCEEEECCCCCHHEECCHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCEE
SCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKTSTADGHNDVELEVTHARPQGVNLAAYKG
EEECHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEECC
INLPDSELPLPSLTEEDLQHLRFVVKYADIAAISFIRNVADVEYLLQALADIGDPVAVER
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEA
CCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHH
AHVPTILATQVLENMAKNGLPSRAEITDAAMALRAECVMLNKGPHINDAIKVLTEMSRKL
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
GASQRKSRLLLRKVKSWEE
CCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEFDQDILQEIKTELDELILELDEVTQTHSEAIGQVSPTHYVGARNLMHYAHLRTKDLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLQQRLSSVGATRLTTTEPAVQARLKAARNVIGAFAGEGPLYPPSDVVDAFEDADEILDE
HHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
HAEILLGEPLPDTPSCIMVTLPTEAATDIELVRGFAKSGMNLARINCAHDDETVWKQMID
HHHEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
NVHTVAEEVGREIRVSMDLAGPKVRTGEIAPGAEVGRARVTRDETGKVLTPAKLWITAHG
HHHHHHHHCCCCEEEEEECCCCCEECCCCCCCCHHCCCCCCCCCCCCEECCEEEEEEECC
SEPVPAPESLPGRPALPIEVTPEWFDKLEIGSVINVPDTRGSRRAFTVTRVFDGAVLAEG
CCCCCCCCCCCCCCCCCEEECHHHHHHHCCCCEECCCCCCCCCEEEEEEEECCCCCCCCC
PQKAYISNGTLLEHNYDRSRVYGIPAVVQRINLKVGDRLILTDEELTYDPSLGSGRTPRI
CCCEEECCCEEEECCCCCHHEECCHHHHHHHHHCCCCEEEEECCCCEECCCCCCCCCCEE
SCTLPQAVDAIKVGHRVLFDDGAIAAVCIDKTSTADGHNDVELEVTHARPQGVNLAAYKG
EEECHHHHHHHHHCCEEEECCCCEEEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEECC
INLPDSELPLPSLTEEDLQHLRFVVKYADIAAISFIRNVADVEYLLQALADIGDPVAVER
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LGLVLKIETIPGYEGLAQILLTGMRHENFGIMIARGDLAVELGFDRMAEVPQLIMALAEA
CCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCEEEEECHHHHHHHHHHHHHHHHH
AHVPTILATQVLENMAKNGLPSRAEITDAAMALRAECVMLNKGPHINDAIKVLTEMSRKL
HCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
GASQRKSRLLLRKVKSWEE
CCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]