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The map label for this gene is fadD2 [H]

Identifier: 62391711

GI number: 62391711

Start: 3041446

End: 3043308

Strand: Reverse

Name: fadD2 [H]

Synonym: cg3179

Alternate gene names: 62391711

Gene position: 3043308-3041446 (Counterclockwise)

Preceding gene: 62391712

Following gene: 62391710

Centisome position: 92.71

GC content: 57.22

Gene sequence:

>1863_bases
ATGGATTTAGATAAAGCGATTGGTTCATTCTTCGATGAGAATGGAGAAATCAACCTTCCTCCATTCCTAACTTTGGCAGC
CATGGGTGAGTTCATGTACCAGGCTGACATCGCTGAAGGCGGCGGGGATAAACCACGCATGCATTTCTGGGACTTCTCCG
AAGACCGCGATGGCAAGCTGATTCAGTACACCCGAAACGAGATCGATACTCGTATCAAGGCTGTAGCAGGCCGTTTGCAG
CAGGTCGCCACCCTGGGTGATCGTGCAGCGATCCTGGCTAACAACAGCCCTGAGTACATTTTCAGCTTCCTCGGCGCGAT
CTACGCTGGCATGGTCCCTGTGCCGCTTTATGATCCAAACGAGCCAGGACACGCAGACCACCTCAACGCTGTTTTCGCAG
ACAGCGAGCCAGTTGTCGTTCTGACCAACTCCAAGTCCGCAGGTGCCGTGCGCAAGCACTTCTCCAGCCTTCCAGCTGCA
GAACGCCCACGCATCCTCTCTGTAGATTCCTTGCCTGATTCTCTCGCGGATTCTTACGAGAACCCAATGCTGACCGAAGC
CGGCCGCCGCCTGGCTGCTCTGCGCCAGTCCGCGCCCATTGATCTGACCGCATTCCTGCAGTACACCTCCGGCTCCACCC
GAACCCCAGCTGGCGTTGTTCTGACCAACCGCTCCATCCTGACCAACGTCTTGCAGATCTTCAGCGCCGCACAGCTGAAA
ACCCCACTGCGCCTGGTTTCATGGCTGCCACTGCACCACGACATGGGCATTATCCTCGCGGCGTTTGTCACTATGCTTGG
CCTGGACAACGAGTTCATGAACCCACGCGATTTCGTGCAGCAGCCTTCCCGCTGGATTAAGCAGCTCAACCGTCGCGAAA
GCGACGTGGACGTTAACGTCTACACCGTGGTTCCTAACTTCGCCCTCGAGCTTGCAGCACGCTACGCAAAGCCAGCAGAG
GGAGAGACCCTGGATCTTTCCGCATTGGATGCCATCATTATCGGTTCCGAGCCAGTCACAGAAAACGCTCTGACCACCTT
CCGTGAAGCTTTCGAGCCTTACGGCCTGCCTGTTCAGACCCTGCGTCCTTCCTACGGTCTTGCAGAAGCATCCCTGCTGG
TCACCACCCCACAGACCGAAAACCGCCCACTGATCTCCTACTTCGACCGCGAGGCCTTGGCCGAAAACCGCGTTGAGCTT
GTAGAAAAGGGCAATAACAAGGCTGTTGCTTTCGTCTCCAACGGCCAGGTTGCAGCCCCACAGCAGCTGGTCATCGTTGA
TTCCGAAACCGGAACCGAGCTGGCAGACGGCCAGATCGGCGAAATCTGGACCCACGGCGAAAACACTGCTGCAGGTTACC
TCGACCGCGAGGAAGACACCGCAGAAACCTTCCGCAACCGTCTGACCACCCGCCTGGAAGAAAACTCCCGCGCAGAAGGT
GCTGCCGACGACAACTACTGGATGGCCACCGGTGACCTCGGCGTCATCGTAGACAACGAGCTCTACATCACCGGTCGTCT
GAAGGACCTCATCGTTGTCGCAGGCCGAAACCACTACCCACAGGACATCGAGTACACCGTCCAGGCTGCTTCCGCACACA
TCCGTGCAGATTCCGTCGCAGCATTCGCAGTCCCAGGCGATGACATTGAAAAGCTCATCATCCTGGCAGAACGCGACACC
ACTGCAAACGAAGCCGACGATGCAGCTGCTGAAGAAGCAATCCGCTCCGCCGTTGGCACTGCACACGGTGTTGTTCCAGA
AGAGATCCGTATCCTCGCACCTGACGAGATCGCGCGTTCCTCCTCCGGAAAGATCGCACGCCGCGTCAACCAGCGCAACT
ACATTCAGGAACAAGCTAACTAG

Upstream 100 bases:

>100_bases
ACGGTCACTCGCTCAAGTAGTGTTAGTTTGCAAAAGTAATAAAATGTTCATCTTTGTCGATGGTCACAATAGTCATTAAA
GAATTGTAAGAGGGGTTCAC

Downstream 100 bases:

>100_bases
TTCTTTGCAGACACCGCAGGGCCCCGATGGATTTTCAACAGAATCCGTCGGGGTTCTTTTGGTTTTGTGCATAGTGGAAA
CATCAGTAGAAACATCAGCG

Product: acyl-CoA synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 620; Mature: 620

Protein sequence:

>620_residues
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKLIQYTRNEIDTRIKAVAGRLQ
QVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPNEPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAA
ERPRILSVDSLPDSLADSYENPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNVYTVVPNFALELAARYAKPAE
GETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQTLRPSYGLAEASLLVTTPQTENRPLISYFDREALAENRVEL
VEKGNNKAVAFVSNGQVAAPQQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
AADDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVAAFAVPGDDIEKLIILAERDT
TANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARSSSGKIARRVNQRNYIQEQAN

Sequences:

>Translated_620_residues
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKLIQYTRNEIDTRIKAVAGRLQ
QVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPNEPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAA
ERPRILSVDSLPDSLADSYENPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNVYTVVPNFALELAARYAKPAE
GETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQTLRPSYGLAEASLLVTTPQTENRPLISYFDREALAENRVEL
VEKGNNKAVAFVSNGQVAAPQQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
AADDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVAAFAVPGDDIEKLIILAERDT
TANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARSSSGKIARRVNQRNYIQEQAN
>Mature_620_residues
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKLIQYTRNEIDTRIKAVAGRLQ
QVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPNEPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAA
ERPRILSVDSLPDSLADSYENPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNVYTVVPNFALELAARYAKPAE
GETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQTLRPSYGLAEASLLVTTPQTENRPLISYFDREALAENRVEL
VEKGNNKAVAFVSNGQVAAPQQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
AADDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVAAFAVPGDDIEKLIILAERDT
TANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARSSSGKIARRVNQRNYIQEQAN

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=530, Percent_Identity=22.8301886792453, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI225735629, Length=534, Percent_Identity=22.4719101123595, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI45827692, Length=534, Percent_Identity=22.4719101123595, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI55749758, Length=458, Percent_Identity=22.2707423580786, Blast_Score=106, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI193202679, Length=594, Percent_Identity=22.5589225589226, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24654656, Length=453, Percent_Identity=21.1920529801325, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 67916; Mature: 67916

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKL
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCE
IQYTRNEIDTRIKAVAGRLQQVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPN
EEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
EPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAAERPRILSVDSLPDSLADSYE
CCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHC
NPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHC
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNV
CHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
YTVVPNFALELAARYAKPAEGETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQT
EEECCHHHHHHHHHHCCCCCCCEEEHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCCHHH
LRPSYGLAEASLLVTTPQTENRPLISYFDREALAENRVELVEKGNNKAVAFVSNGQVAAP
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
QQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
CEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AADDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVA
CCCCCEEEEECCEEEEECCEEEEEECCEEEEEEECCCCCCCCHHHHEEHHHHEEECCCEE
AFAVPGDDIEKLIILAERDTTANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARS
EEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHHCC
SSGKIARRVNQRNYIQEQAN
CCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDLDKAIGSFFDENGEINLPPFLTLAAMGEFMYQADIAEGGGDKPRMHFWDFSEDRDGKL
CCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCE
IQYTRNEIDTRIKAVAGRLQQVATLGDRAAILANNSPEYIFSFLGAIYAGMVPVPLYDPN
EEEHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCC
EPGHADHLNAVFADSEPVVVLTNSKSAGAVRKHFSSLPAAERPRILSVDSLPDSLADSYE
CCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHC
NPMLTEAGRRLAALRQSAPIDLTAFLQYTSGSTRTPAGVVLTNRSILTNVLQIFSAAQLK
CCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEEEECHHHHHHHHHHHHHHHHC
TPLRLVSWLPLHHDMGIILAAFVTMLGLDNEFMNPRDFVQQPSRWIKQLNRRESDVDVNV
CHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEE
YTVVPNFALELAARYAKPAEGETLDLSALDAIIIGSEPVTENALTTFREAFEPYGLPVQT
EEECCHHHHHHHHHHCCCCCCCEEEHHHHCEEEECCCCCCHHHHHHHHHHHCCCCCCHHH
LRPSYGLAEASLLVTTPQTENRPLISYFDREALAENRVELVEKGNNKAVAFVSNGQVAAP
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
QQLVIVDSETGTELADGQIGEIWTHGENTAAGYLDREEDTAETFRNRLTTRLEENSRAEG
CEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
AADDNYWMATGDLGVIVDNELYITGRLKDLIVVAGRNHYPQDIEYTVQAASAHIRADSVA
CCCCCEEEEECCEEEEECCEEEEEECCEEEEEEECCCCCCCCHHHHEEHHHHEEECCCEE
AFAVPGDDIEKLIILAERDTTANEADDAAAEEAIRSAVGTAHGVVPEEIRILAPDEIARS
EEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHEEEECCHHHHCC
SSGKIARRVNQRNYIQEQAN
CCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]