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The map label for this gene is metB [H]

Identifier: 62391621

GI number: 62391621

Start: 2945363

End: 2946511

Strand: Reverse

Name: metB [H]

Synonym: cg3086

Alternate gene names: 62391621

Gene position: 2946511-2945363 (Counterclockwise)

Preceding gene: 62391622

Following gene: 62391616

Centisome position: 89.76

GC content: 51.35

Gene sequence:

>1149_bases
ATGAATTTTTACCCACCATCTGTACCTATTAACCCTGCGTGGCGTCCACCCACAGTAACTGTGCAAGCGGGACGGCCAGC
CAGAACTCCTGGTGCGCCGATGAACCCACCTATCACGTTGTCCAGCACTTATGTTCATGATTCAGAAAAAGCTTATGGGC
GCGATGGCAATGATGGATGGGGTGCATTTGAGGCTGCCATGGGAACTCTAGATGGTGGGTTCGCGGTATCTTATTCTTCA
GGTTTGGCAGCGGCAACGTCGATTGCTGATTTGGTTCCTACTGGTGGCACAGTTGTTTTACCTAAAGCTGCCTATTATGG
CGTGACCAATATTTTCGCCAGGATGGAAGCCCGCGGAAGGCTGAAGGTTCGAACTGTTGATGCAGACAATACCGAAGAAG
TGATTGCTGCTGCTCAAGGTGCAGATGTGGTGTGGGTGGAATCGATCGCTAATCCGACGATGGTGGTAGCTGATATCCCT
GCAATAGTCGACGGTGTGCGTGGGCTTGGAGTTTTGACTGTCGTTGACGCGACTTTCGCAACGCCACTTCGTCAACGTCC
ATTGGAACTTGGTGCTGATATTGTGCTTTACTCGGCAACCAAACTTATCGGTGGACACTCTGATCTTCTTCTTGGAGTCG
CAGTGTGCAAGTCTGAGCACCATGCGCAGTTTCTTGCCACTCACCGTCATGATCATGGTTCAGTGCCGGGAGGTCTTGAA
GCGTTTCTTGCTCTCCGTGGATTGTATTCCTTGGCGGTGCGTCTTGATCGAGCAGAATCCAACGCAGCAGAACTTTCGCG
GCGACTTAACGCGCATCCTTCGGTTACCCGCGTCAATTATCCAGGACTTCCTGATGATCCCCAACATGAAAAAGCCGTGC
GAGTCCTACCCTCTGGATGTGGAAACATGTTGTCATTTGAGCTTGATGCAACACCTGAACGAACTGATGAGATTCTCGAA
AGCCTGTCACTTTTAACCCACGCGACCAGTTGGGGAGGTGTGGAAACAGCCATTGAACGTCGCACCAGGCGGGATGCTGA
AGTGGTGGCAGGAGTACCGATGACTCTTTGCCGCGTTTCCGTAGGAATTGAAGACGTTGAAGATCTATGGGAAGACCTCA
ACGCCTCAATCGACAAAGTTCTGGGTTAG

Upstream 100 bases:

>100_bases
AGATACCGACTTTGCTAATCCCACCACCGTAGCCACTAGGTATTTAGTTGCCTTGGACAAGCGTGTACCTCGTGCTTGAT
ATAACAAGTAAGGAAGCCTG

Downstream 100 bases:

>100_bases
AACTCGTAGCCAGTAACCAGACCTTCAGTGTTTGGTTGCCACTCCAGTGCTGGGGCGACATGATCAGCGAAGTTCTTCAG
GATCGACGCGTTGATCTCAA

Product: L,L-cystathionine gamma-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNFYPPSVPINPAWRPPTVTVQAGRPARTPGAPMNPPITLSSTYVHDSEKAYGRDGNDGWGAFEAAMGTLDGGFAVSYSS
GLAAATSIADLVPTGGTVVLPKAAYYGVTNIFARMEARGRLKVRTVDADNTEEVIAAAQGADVVWVESIANPTMVVADIP
AIVDGVRGLGVLTVVDATFATPLRQRPLELGADIVLYSATKLIGGHSDLLLGVAVCKSEHHAQFLATHRHDHGSVPGGLE
AFLALRGLYSLAVRLDRAESNAAELSRRLNAHPSVTRVNYPGLPDDPQHEKAVRVLPSGCGNMLSFELDATPERTDEILE
SLSLLTHATSWGGVETAIERRTRRDAEVVAGVPMTLCRVSVGIEDVEDLWEDLNASIDKVLG

Sequences:

>Translated_382_residues
MNFYPPSVPINPAWRPPTVTVQAGRPARTPGAPMNPPITLSSTYVHDSEKAYGRDGNDGWGAFEAAMGTLDGGFAVSYSS
GLAAATSIADLVPTGGTVVLPKAAYYGVTNIFARMEARGRLKVRTVDADNTEEVIAAAQGADVVWVESIANPTMVVADIP
AIVDGVRGLGVLTVVDATFATPLRQRPLELGADIVLYSATKLIGGHSDLLLGVAVCKSEHHAQFLATHRHDHGSVPGGLE
AFLALRGLYSLAVRLDRAESNAAELSRRLNAHPSVTRVNYPGLPDDPQHEKAVRVLPSGCGNMLSFELDATPERTDEILE
SLSLLTHATSWGGVETAIERRTRRDAEVVAGVPMTLCRVSVGIEDVEDLWEDLNASIDKVLG
>Mature_382_residues
MNFYPPSVPINPAWRPPTVTVQAGRPARTPGAPMNPPITLSSTYVHDSEKAYGRDGNDGWGAFEAAMGTLDGGFAVSYSS
GLAAATSIADLVPTGGTVVLPKAAYYGVTNIFARMEARGRLKVRTVDADNTEEVIAAAQGADVVWVESIANPTMVVADIP
AIVDGVRGLGVLTVVDATFATPLRQRPLELGADIVLYSATKLIGGHSDLLLGVAVCKSEHHAQFLATHRHDHGSVPGGLE
AFLALRGLYSLAVRLDRAESNAAELSRRLNAHPSVTRVNYPGLPDDPQHEKAVRVLPSGCGNMLSFELDATPERTDEILE
SLSLLTHATSWGGVETAIERRTRRDAEVVAGVPMTLCRVSVGIEDVEDLWEDLNASIDKVLG

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=348, Percent_Identity=35.632183908046, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI299473759, Length=347, Percent_Identity=33.4293948126801, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI25453487, Length=346, Percent_Identity=31.2138728323699, Blast_Score=149, Evalue=5e-36,
Organism=Escherichia coli, GI1790375, Length=378, Percent_Identity=36.2433862433862, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1789383, Length=392, Percent_Identity=29.8469387755102, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI71980708, Length=373, Percent_Identity=34.5844504021448, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=346, Percent_Identity=33.2369942196532, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17538055, Length=346, Percent_Identity=33.2369942196532, Blast_Score=174, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6319307, Length=384, Percent_Identity=31.25, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321904, Length=367, Percent_Identity=32.425068119891, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6323333, Length=356, Percent_Identity=30.0561797752809, Blast_Score=116, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321254, Length=414, Percent_Identity=29.7101449275362, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=336, Percent_Identity=29.4642857142857, Blast_Score=106, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922554, Length=378, Percent_Identity=30.952380952381, Blast_Score=180, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 40602; Mature: 40602

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFYPPSVPINPAWRPPTVTVQAGRPARTPGAPMNPPITLSSTYVHDSEKAYGRDGNDGW
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEEECCHHHCCCCCCCCC
GAFEAAMGTLDGGFAVSYSSGLAAATSIADLVPTGGTVVLPKAAYYGVTNIFARMEARGR
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCC
LKVRTVDADNTEEVIAAAQGADVVWVESIANPTMVVADIPAIVDGVRGLGVLTVVDATFA
EEEEEECCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECHHHHHHHCCCEEEEEHHHHHH
TPLRQRPLELGADIVLYSATKLIGGHSDLLLGVAVCKSEHHAQFLATHRHDHGSVPGGLE
CHHHHCCHHHCCCEEEEEHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHH
AFLALRGLYSLAVRLDRAESNAAELSRRLNAHPSVTRVNYPGLPDDPQHEKAVRVLPSGC
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHCCCCC
GNMLSFELDATPERTDEILESLSLLTHATSWGGVETAIERRTRRDAEVVAGVPMTLCRVS
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHH
VGIEDVEDLWEDLNASIDKVLG
CCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFYPPSVPINPAWRPPTVTVQAGRPARTPGAPMNPPITLSSTYVHDSEKAYGRDGNDGW
CCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEEECCHHHCCCCCCCCC
GAFEAAMGTLDGGFAVSYSSGLAAATSIADLVPTGGTVVLPKAAYYGVTNIFARMEARGR
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHCCCC
LKVRTVDADNTEEVIAAAQGADVVWVESIANPTMVVADIPAIVDGVRGLGVLTVVDATFA
EEEEEECCCCHHHHHHHCCCCCEEEEECCCCCEEEEEECHHHHHHHCCCEEEEEHHHHHH
TPLRQRPLELGADIVLYSATKLIGGHSDLLLGVAVCKSEHHAQFLATHRHDHGSVPGGLE
CHHHHCCHHHCCCEEEEEHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHH
AFLALRGLYSLAVRLDRAESNAAELSRRLNAHPSVTRVNYPGLPDDPQHEKAVRVLPSGC
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHCCCCC
GNMLSFELDATPERTDEILESLSLLTHATSWGGVETAIERRTRRDAEVVAGVPMTLCRVS
CCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHCCCCHHHHHHH
VGIEDVEDLWEDLNASIDKVLG
CCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]