Click here to switch to the map view.

The map label for this gene is purA

Identifier: 62391601

GI number: 62391601

Start: 2923737

End: 2925029

Strand: Reverse

Name: purA

Synonym: cg3063

Alternate gene names: 62391601

Gene position: 2925029-2923737 (Counterclockwise)

Preceding gene: 62391604

Following gene: 62391600

Centisome position: 89.1

GC content: 56.92

Gene sequence:

>1293_bases
ATGGCTGCAATCGTTATTGTCGGCGCTCAATGGGGCGATGAAGGCAAAGGTAAGGCCACGGATATTCTCGGCGGACTCGT
CGATTACGTGGTTAAGCCCAATGGCGGTAACAACGCTGGACACACTGTTGTGGTCGGCGGCGAGAAGTACGAGCTAAAGC
TCCTTCCTGCCGGCGTCCTCTCCGAAACGGCCACCCCAATTTTGGGCAACGGCGTTGTGATCAACCTTGAGGCACTGTTC
GAAGAAATCGACGGCCTTGAGGCTCGCGGTGCGGATGCATCCCGCCTGCGCATCTCTGCAAACGCTCACCTGGTTGCTCC
ATACCACCAGGTGATGGACCGTGTTCAGGAACGCTTCCTGGGCAAGCGCGCAATCGGCACCACCGGCCGTGGCATCGGCC
CAACCTACGCGGACAAAGTATCCCGCGTGGGAATCCGTGTTCAAGACATTTTCGACGAATCCATCCTTCGTCAAAAAGTC
GAATCCGCCCTGGATTACAAAAACCAGGTGCTGGTGAAGATGTACAACCGCAAGGCCATCGTCGCTGAGGAAATCGTGCA
GTACTTCCTCTCCTACGCTGATCGTCTGCGCCCCATGGTCATCGATGCCACCTTGGTGCTCAACGAGGCACTTGATCAGG
GCAAGCACGTTCTTATGGAAGGTGGCCAGGCAACCATGCTCGACGTGGACCACGGCACCTACCCATTCGTCACCTCCTCC
AACCCAACCGCCGGTGGCGCAAGTGTTGGTTCAGGTATCGGCCCAACCAAGATCACCAGCTCCTTGGGTATCATCAAGGC
CTACACCACTCGTGTTGGTGCCGGCCCATTCCCAACTGAGCTGTTTGATAAGTGGGGCGAGTACCTGCAGACCGTCGGTG
GCGAGGTCGGCGTGAACACCGGCCGTAAGCGTCGCTGTGGCTGGTACGACTCCGTGATTGCTCGTTACGCATCCCGCGTC
AACGGATTCACCGACTACTTCCTGACCAAGCTAGACGTGCTCACCGGCATCGGTGAAATCCCAATCTGCGTAGCTTACGA
CGTTGATGGTGTTCGCCACGATGAAATGCCACTGACCCAGTCAGAGTTCCACCACGCAACCCCAATCTTTGAAACCATGC
CTGCATGGGACGAAGACATCACCGACTGCAAGACCTTCGAGGATCTTCCACAAAAGGCCCAGGACTACGTCCGACGTCTG
GAAGAACTCTCTGGTGCTCGCTTCTCCTACATCGGTGTTGGACCTGGTCGCGATCAGACCATCGTCCTGCATGACGTACT
AGCTGACAACTAG

Upstream 100 bases:

>100_bases
GATGATTAAAAACCTCACATGGGATAGTAAGATGAAATAACGTCTGTGTGCGCACACGCGTGCATACTCCAACTTCATGG
ATCGGATGTGACGTAAACCA

Downstream 100 bases:

>100_bases
TACTGATAAACATCAGTGAGTTTCTCCACTCCCCGGAAGTCGGCCTGAGAAATCAGATTCCAACAGTCGGGGAGTTTTTC
TTTTGGCAAGTCCATTAATG

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MAAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSETATPILGNGVVINLEALF
EEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFLGKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKV
ESALDYKNQVLVKMYNRKAIVAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNTGRKRRCGWYDSVIARYASRV
NGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQSEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRL
EELSGARFSYIGVGPGRDQTIVLHDVLADN

Sequences:

>Translated_430_residues
MAAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSETATPILGNGVVINLEALF
EEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFLGKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKV
ESALDYKNQVLVKMYNRKAIVAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNTGRKRRCGWYDSVIARYASRV
NGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQSEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRL
EELSGARFSYIGVGPGRDQTIVLHDVLADN
>Mature_429_residues
AAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVLSETATPILGNGVVINLEALFE
EIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFLGKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKVE
SALDYKNQVLVKMYNRKAIVAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSSN
PTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNTGRKRRCGWYDSVIARYASRVN
GFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQSEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRLE
ELSGARFSYIGVGPGRDQTIVLHDVLADN

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=46.0280373831776, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=44.6511627906977, Blast_Score=338, Evalue=7e-93,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=45.7286432160804, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1790620, Length=424, Percent_Identity=47.6415094339623, Blast_Score=406, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.3611111111111, Blast_Score=327, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.3611111111111, Blast_Score=327, Evalue=8e-90,
Organism=Saccharomyces cerevisiae, GI6324109, Length=432, Percent_Identity=43.0555555555556, Blast_Score=361, Evalue=1e-100,
Organism=Drosophila melanogaster, GI21356033, Length=424, Percent_Identity=44.5754716981132, Blast_Score=355, Evalue=3e-98,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_CORGL (Q8NM16)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927156
- RefSeq:   NP_601960.1
- RefSeq:   YP_227003.1
- ProteinModelPortal:   Q8NM16
- SMR:   Q8NM16
- GeneID:   1020710
- GeneID:   3345002
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg3063
- KEGG:   cgl:NCgl2669
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q8NM16
- ProtClustDB:   PRK01117
- BioCyc:   CGLU196627:CG3063-MONOMER
- BRENDA:   6.3.4.4
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 46770; Mature: 46638

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVL
CEEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEECCCEEEEEEECCHHH
SETATPILGNGVVINLEALFEEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFL
HHCCCCEECCCEEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHH
GKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKVESALDYKNQVLVKMYNRKAI
CCHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEECC
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNT
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRKRRCGWYDSVIARYASRVNGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCH
SEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRLEELSGARFSYIGVGPGRDQT
HHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
IVLHDVLADN
EEEEECCCCC
>Mature Secondary Structure 
AAIVIVGAQWGDEGKGKATDILGGLVDYVVKPNGGNNAGHTVVVGGEKYELKLLPAGVL
EEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCCCCCCCCEEEECCCEEEEEEECCHHH
SETATPILGNGVVINLEALFEEIDGLEARGADASRLRISANAHLVAPYHQVMDRVQERFL
HHCCCCEECCCEEEEHHHHHHHHCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHHHHHH
GKRAIGTTGRGIGPTYADKVSRVGIRVQDIFDESILRQKVESALDYKNQVLVKMYNRKAI
CCHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
VAEEIVQYFLSYADRLRPMVIDATLVLNEALDQGKHVLMEGGQATMLDVDHGTYPFVTSS
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCEEECC
NPTAGGASVGSGIGPTKITSSLGIIKAYTTRVGAGPFPTELFDKWGEYLQTVGGEVGVNT
CCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
GRKRRCGWYDSVIARYASRVNGFTDYFLTKLDVLTGIGEIPICVAYDVDGVRHDEMPLTQ
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCH
SEFHHATPIFETMPAWDEDITDCKTFEDLPQKAQDYVRRLEELSGARFSYIGVGPGRDQT
HHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCE
IVLHDVLADN
EEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626