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The map label for this gene is mesJ

Identifier: 62391535

GI number: 62391535

Start: 2843804

End: 2844748

Strand: Reverse

Name: mesJ

Synonym: cg2986

Alternate gene names: 62391535

Gene position: 2844748-2843804 (Counterclockwise)

Preceding gene: 62391536

Following gene: 62391534

Centisome position: 86.66

GC content: 59.89

Gene sequence:

>945_bases
GTGGCATCCCTCATCGGGAATCTTGAGCTGCCTAGGGTAAGCCCTAATTTCTTGGAATTACGCAAGGCGGTGCGCCCTTA
CCTGAAAGAGCATGTGCACATTGGGTTGTCGGGCGGGCCGGATTCATTGGCGCTGGTGGCTGCTGTGCTCGCGGAGAAAT
CCCAGGTAACGGCGATTTGTATCGATCATAATCTGCAGACCGGTTCTGCTGAAGTCACGCACAACGCTGCTGCGATGGCG
CGCCACATGGGCGCACAGGCGATCGTGAAGAGCATCGAGGTCGCGCCGGGGGAGGGGATGGAGGCCGCCGCCAGGGAGGC
TCGGTACGCGGCTTTTGCGCAGCTCACCGATGAGATTTGGGTGGCGCACACCATGGATGATCAAGCCGAGACCTATCTCC
TTGGCGGTTTGCGGGGGAATCCCGCGGGCATGAAAGATGCTTCTCGACGCCCCGAGCTATCCATTATTCGACCCCTTCTG
GGGGCTCGGCGTGCGCACACGCACGGGGCGTGCGTGGAGTTGGGGTTGAAACCGTGGCACGATCCGCAAAATTTTGACGA
TGCCTTTCGGCGGGTAGCCATCCGAAACCAGGTGATTCCTCTTCTTGCGCAGGTGCACGGGGGAGACCCTGTACCTGGTT
TGGCACTTGCGGCGCGACGCGCTGTGGAGGATGCCGAAGTGGTGGAGGGCGACGTCGAGAAGCGGCGTTTAGAGTGGCAG
GACGGTTTTCCTGTGACGTTGGCCGGGGAACCTACGGGCCTTAGGCGACGCATGTTGGCGGATTTTTTGCGTGGGGAAGG
CATCGCTGTGACCTCGAGGAAACTCGACGCCATTGACCGATTGCTCACCGATTGGCGCGGACAGGGTGGCGTAGCCGTGG
GTAAAAGCGATAATGGAAGGTTGGAAGTGGTGCGGCAAAGTGGCAAGCTTAAGATCACTGATTGA

Upstream 100 bases:

>100_bases
GGGCGTGTGTTTACCTTTGCTTTTGTGTCTAATGGTTCCGCGATTGTGCCGGCGCGTGAGGCTTTGGATGAGATGGCGTC
GATTCTGAGGGACTTTTAAG

Downstream 100 bases:

>100_bases
CACCTGAATCTACAACACAAGGGGAACGCGACGATGAGCAACAACGTAGAAATGGCCGACCACAAAGATCTCAATGTTCC
AGCCAACCCATACGGCACCG

Product: PP family ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MASLIGNLELPRVSPNFLELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQTGSAEVTHNAAAMA
RHMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLL
GARRAHTHGACVELGLKPWHDPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQ
DGFPVTLAGEPTGLRRRMLADFLRGEGIAVTSRKLDAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD

Sequences:

>Translated_314_residues
MASLIGNLELPRVSPNFLELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQTGSAEVTHNAAAMA
RHMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLL
GARRAHTHGACVELGLKPWHDPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQ
DGFPVTLAGEPTGLRRRMLADFLRGEGIAVTSRKLDAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD
>Mature_313_residues
ASLIGNLELPRVSPNFLELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAICIDHNLQTGSAEVTHNAAAMAR
HMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIWVAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLLG
ARRAHTHGACVELGLKPWHDPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQD
GFPVTLAGEPTGLRRRMLADFLRGEGIAVTSRKLDAIDRLLTDWRGQGGVAVGKSDNGRLEVVRQSGKLKITD

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=188, Percent_Identity=33.5106382978723, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_CORGL (Q6M2E8)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927156
- RefSeq:   NP_601894.1
- RefSeq:   YP_226937.1
- ProteinModelPortal:   Q6M2E8
- SMR:   Q6M2E8
- GeneID:   1020645
- GeneID:   3344574
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg2986
- KEGG:   cgl:NCgl2605
- HOGENOM:   HBG692699
- OMA:   HIGLSGG
- ProtClustDB:   CLSK633922
- BioCyc:   CGLU196627:CG2986-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 33957; Mature: 33826

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLIGNLELPRVSPNFLELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAIC
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEE
IDHNLQTGSAEVTHNAAAMARHMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIW
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHE
VAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLKPWH
EEECCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCC
DPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCC
DGFPVTLAGEPTGLRRRMLADFLRGEGIAVTSRKLDAIDRLLTDWRGQGGVAVGKSDNGR
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCCE
LEVVRQSGKLKITD
EEEEECCCCEEECC
>Mature Secondary Structure 
ASLIGNLELPRVSPNFLELRKAVRPYLKEHVHIGLSGGPDSLALVAAVLAEKSQVTAIC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCEEEEE
IDHNLQTGSAEVTHNAAAMARHMGAQAIVKSIEVAPGEGMEAAAREARYAAFAQLTDEIW
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHE
VAHTMDDQAETYLLGGLRGNPAGMKDASRRPELSIIRPLLGARRAHTHGACVELGLKPWH
EEECCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCC
DPQNFDDAFRRVAIRNQVIPLLAQVHGGDPVPGLALAARRAVEDAEVVEGDVEKRRLEWQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCC
DGFPVTLAGEPTGLRRRMLADFLRGEGIAVTSRKLDAIDRLLTDWRGQGGVAVGKSDNGR
CCCCEEECCCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCCE
LEVVRQSGKLKITD
EEEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626