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The map label for this gene is lepA [H]

Identifier: 62391184

GI number: 62391184

Start: 2454222

End: 2456069

Strand: Reverse

Name: lepA [H]

Synonym: cg2571

Alternate gene names: 62391184

Gene position: 2456069-2454222 (Counterclockwise)

Preceding gene: 62391187

Following gene: 62391183

Centisome position: 74.82

GC content: 53.63

Gene sequence:

>1848_bases
ATGGCAGAGAAATTTGCAGAAACAACATTTACGGATCCAGCCAGGATTCGTAACTTCTGCATCATTGCCCACATTGACCA
CGGTAAATCTACGCTCGCTGACCGTATCCTGCAGCTGTCTAACGTTGTGGATGCCCGCGATATGCGTGATCAGTACCTGG
ACAACATGGACATCGAACGTGAACGTGGCATTACCATTAAGGCTCAGAACGTTCGCCTGCCATGGATTCCTCGCAGTGGT
GAGTACGAGGGCCAGCAGATCGTCATGCAGATGATCGATACGCCAGGCCACGTGGACTTCACCTATGAAGTGTCTCGGGC
GCTTGAAGCGTGTGAAGGCGCGATTTTGCTTGTTGATGCAGCGCAGGGCATTGAAGCCCAGACCTTGGCAAACTTGTACT
TGGCTATGGAAAACGATCTTGAGATCATCCCTGTGCTGAACAAGATTGACCTTCCAGCGGCGGATCCAGACAAGTACGCG
TTGGAGATCGCCAACATTGTGGGTTGTGAACCTGAAGATGTGTTGCGCGTGTCCGGTAAAACTGGCATGGGTGTCCCTGA
GCTTCTGGATAAGGTCGTTGAACTTATCCCAGCACCTACCTCTGAATTTGAGGAAGACGCCCCAGCTCGTGCGATGATTT
TCGACTCTGTCTATGACACCTACCGCGGCGTGGTTACCTACATCCGCATGATGGACGGCAAGCTGACACCTCGCCAAAAG
ATCAAGATGATGTCCACCGGCGCCACCCACGAGTTGCTGGAAATCGGCATCGTGAGCCCCACCCCTAAAAAGTGTGTGGG
TCTTGGACCTGGCGAGGTTGGTTACCTGATCACCGGTGTGAAGGACGTGCGCCAATCTAAGGTGGGCGATACCGTCACGT
GGGCAATTCATGGAGCTGAGCAGCCACTGCGCGGTTACCAGGAACCAACACCGATGGTTTACTCGGGCTTGTTCCCGATT
TCCCAAGCGGATTTCCCCGACTTGCGCGATGCGCTTGAAAAGCTGCAGCTTAACGACGCCTCCCTCACGTACGAACCCGA
AACGTCCGTAGCACTGGGCTTTGGTTTCCGATGTGGCTTCCTCGGACTGCTGCACATGGAAATCACCCGTGACCGACTCG
AACGTGAGTTTGGCCTTGATCTGATTTCTACCGCGCCATCTGTTAACTACCGCGTTATTGATGAGGCGGGCAAGGAATTC
CGCGTCCACAACCCATCTGACTGGCCTGGCGGAAAGCTCAGTGAAGTTTACGAGCCCATCGTGAAGGTCACCATCATTGT
TCCTAGCGAATTTGTGGGACCGACAATGGAGCTGTGCCAGACTAAGCGTGGTCAGATGGGTGGCATGGACTACCTTTCGG
AGGACCGCGTGGAGCTGCGCTACACCATGCCTTTGGGTGAGATCATCTTTGACTTCTTCGATATGTTGAAGTCTCGCACC
AAGGGTTACGCTTCGCTGAACTACGAGGAAGCTGGCGAGCAGACTGCCGACCTGGTCAAGGTAGATATCTTGCTCCAAGG
TGAACCTGTGGATGCATTCTCTGCGATCGTGCACCGCGATAATGCGCAGTGGTACGGAAACAAGATGACTGTGAAGCTGA
AGGAACTGATCCCTCGCCAGCAGTTCGAAGTTCCTGTGCAGGCAGCCATTGGTTCCAAGGTTATCGCTCGTGAAAACATT
CGTGCACTGCGCAAGGACGTGTTGGCGAAGTGTTACGGTGGCGATATTTCCCGTAAGCGCAAGCTTCTGGAAAAGCAGAA
GGCTGGTAAGAAGCGCATGAAGAACATCGGTTCGGTCGAGGTTCCTCAGGAAGCATTCGTAGCAGCACTGTCTACCGACG
AGGCATAA

Upstream 100 bases:

>100_bases
AAAACTGCTGATGGTTTGGCAGTTCAGGGCTGTTTAACTGTTTGGGTTTGCTCAGGTAGAGTAGGACACCGTTATTTAAT
TGAAGGGACCCCTGACGCAT

Downstream 100 bases:

>100_bases
AAAACTTTAGCCTCTTTTAGCGCCCCGCTGAGCTTGAAGCTCAGCGGGGCGTTGTCGTGCTTCGGTGTGGGGGAGCGTCG
AAAAGCGAAAGTATCTAAGG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 615; Mature: 614

Protein sequence:

>615_residues
MAEKFAETTFTDPARIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIERERGITIKAQNVRLPWIPRSG
EYEGQQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYA
LEIANIVGCEPEDVLRVSGKTGMGVPELLDKVVELIPAPTSEFEEDAPARAMIFDSVYDTYRGVVTYIRMMDGKLTPRQK
IKMMSTGATHELLEIGIVSPTPKKCVGLGPGEVGYLITGVKDVRQSKVGDTVTWAIHGAEQPLRGYQEPTPMVYSGLFPI
SQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGFLGLLHMEITRDRLEREFGLDLISTAPSVNYRVIDEAGKEF
RVHNPSDWPGGKLSEVYEPIVKVTIIVPSEFVGPTMELCQTKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDMLKSRT
KGYASLNYEEAGEQTADLVKVDILLQGEPVDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENI
RALRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGSVEVPQEAFVAALSTDEA

Sequences:

>Translated_615_residues
MAEKFAETTFTDPARIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIERERGITIKAQNVRLPWIPRSG
EYEGQQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYA
LEIANIVGCEPEDVLRVSGKTGMGVPELLDKVVELIPAPTSEFEEDAPARAMIFDSVYDTYRGVVTYIRMMDGKLTPRQK
IKMMSTGATHELLEIGIVSPTPKKCVGLGPGEVGYLITGVKDVRQSKVGDTVTWAIHGAEQPLRGYQEPTPMVYSGLFPI
SQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGFLGLLHMEITRDRLEREFGLDLISTAPSVNYRVIDEAGKEF
RVHNPSDWPGGKLSEVYEPIVKVTIIVPSEFVGPTMELCQTKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDMLKSRT
KGYASLNYEEAGEQTADLVKVDILLQGEPVDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENI
RALRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGSVEVPQEAFVAALSTDEA
>Mature_614_residues
AEKFAETTFTDPARIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIERERGITIKAQNVRLPWIPRSGE
YEGQQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDAAQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYAL
EIANIVGCEPEDVLRVSGKTGMGVPELLDKVVELIPAPTSEFEEDAPARAMIFDSVYDTYRGVVTYIRMMDGKLTPRQKI
KMMSTGATHELLEIGIVSPTPKKCVGLGPGEVGYLITGVKDVRQSKVGDTVTWAIHGAEQPLRGYQEPTPMVYSGLFPIS
QADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGFLGLLHMEITRDRLEREFGLDLISTAPSVNYRVIDEAGKEFR
VHNPSDWPGGKLSEVYEPIVKVTIIVPSEFVGPTMELCQTKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDMLKSRTK
GYASLNYEEAGEQTADLVKVDILLQGEPVDAFSAIVHRDNAQWYGNKMTVKLKELIPRQQFEVPVQAAIGSKVIARENIR
ALRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGSVEVPQEAFVAALSTDEA

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=609, Percent_Identity=45.9770114942529, Blast_Score=583, Evalue=1e-166,
Organism=Homo sapiens, GI94966754, Length=140, Percent_Identity=40, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI25306283, Length=152, Percent_Identity=42.1052631578947, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI25306287, Length=152, Percent_Identity=42.1052631578947, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI19923640, Length=152, Percent_Identity=42.1052631578947, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=34.6666666666667, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=35.1724137931034, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310132016, Length=117, Percent_Identity=36.7521367521368, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310110807, Length=117, Percent_Identity=36.7521367521368, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI310123363, Length=117, Percent_Identity=36.7521367521368, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI217272892, Length=140, Percent_Identity=32.8571428571429, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI217272894, Length=140, Percent_Identity=32.8571428571429, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI53729339, Length=220, Percent_Identity=28.1818181818182, Blast_Score=67, Evalue=6e-11,
Organism=Homo sapiens, GI53729337, Length=220, Percent_Identity=28.1818181818182, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=54.606365159129, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI48994988, Length=485, Percent_Identity=27.8350515463918, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789738, Length=186, Percent_Identity=34.4086021505376, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=167, Percent_Identity=29.940119760479, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789559, Length=287, Percent_Identity=26.1324041811847, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=624, Percent_Identity=40.3846153846154, Blast_Score=456, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI71988811, Length=142, Percent_Identity=38.7323943661972, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=142, Percent_Identity=38.7323943661972, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=151, Percent_Identity=41.0596026490066, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=145, Percent_Identity=36.551724137931, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=30.9677419354839, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17552882, Length=152, Percent_Identity=29.6052631578947, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556456, Length=137, Percent_Identity=34.3065693430657, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=43.1893687707641, Blast_Score=499, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6323098, Length=203, Percent_Identity=33.0049261083744, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324707, Length=144, Percent_Identity=36.8055555555556, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6320593, Length=144, Percent_Identity=36.8055555555556, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322359, Length=131, Percent_Identity=36.6412213740458, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=39.041095890411, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=252, Percent_Identity=26.984126984127, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=608, Percent_Identity=43.5855263157895, Blast_Score=522, Evalue=1e-148,
Organism=Drosophila melanogaster, GI28574573, Length=138, Percent_Identity=42.7536231884058, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24582462, Length=148, Percent_Identity=37.1621621621622, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=34.6938775510204, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=34.6938775510204, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=34.6938775510204, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21357743, Length=142, Percent_Identity=32.3943661971831, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221458488, Length=161, Percent_Identity=33.5403726708075, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 68550; Mature: 68418

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKFAETTFTDPARIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIER
CCCHHHCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ERGITIKAQNVRLPWIPRSGEYEGQQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDA
HCCCEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCEEEEEC
AQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYALEIANIVGCEPEDVLRVSGK
CCCCCHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEECCC
TGMGVPELLDKVVELIPAPTSEFEEDAPARAMIFDSVYDTYRGVVTYIRMMDGKLTPRQK
CCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IKMMSTGATHELLEIGIVSPTPKKCVGLGPGEVGYLITGVKDVRQSKVGDTVTWAIHGAE
HHHHHCCCCHHHHEEECCCCCCHHHCCCCCCCCCCEEECHHHHHHHHCCCEEEEEEECCC
QPLRGYQEPTPMVYSGLFPISQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGF
CCCCCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHH
LGLLHMEITRDRLEREFGLDLISTAPSVNYRVIDEAGKEFRVHNPSDWPGGKLSEVYEPI
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHHH
VKVTIIVPSEFVGPTMELCQTKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDMLKSRT
EEEEEEECCHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHC
KGYASLNYEEAGEQTADLVKVDILLQGEPVDAFSAIVHRDNAQWYGNKMTVKLKELIPRQ
CCCEECCHHHCCCHHHCEEEEEEEEECCCHHHHHHHHHCCCCCEECCEEEEEHHHHCCCH
QFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGSVE
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VPQEAFVAALSTDEA
CCHHHHHHHHCCCCC
>Mature Secondary Structure 
AEKFAETTFTDPARIRNFCIIAHIDHGKSTLADRILQLSNVVDARDMRDQYLDNMDIER
CCHHHCCCCCCHHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ERGITIKAQNVRLPWIPRSGEYEGQQIVMQMIDTPGHVDFTYEVSRALEACEGAILLVDA
HCCCEEEECCEEECCCCCCCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCEEEEEC
AQGIEAQTLANLYLAMENDLEIIPVLNKIDLPAADPDKYALEIANIVGCEPEDVLRVSGK
CCCCCHHHHHHHHEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHCCCHHHHEEECCC
TGMGVPELLDKVVELIPAPTSEFEEDAPARAMIFDSVYDTYRGVVTYIRMMDGKLTPRQK
CCCCHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
IKMMSTGATHELLEIGIVSPTPKKCVGLGPGEVGYLITGVKDVRQSKVGDTVTWAIHGAE
HHHHHCCCCHHHHEEECCCCCCHHHCCCCCCCCCCEEECHHHHHHHHCCCEEEEEEECCC
QPLRGYQEPTPMVYSGLFPISQADFPDLRDALEKLQLNDASLTYEPETSVALGFGFRCGF
CCCCCCCCCCCHHEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCEEEECCHHHHH
LGLLHMEITRDRLEREFGLDLISTAPSVNYRVIDEAGKEFRVHNPSDWPGGKLSEVYEPI
HHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHHH
VKVTIIVPSEFVGPTMELCQTKRGQMGGMDYLSEDRVELRYTMPLGEIIFDFFDMLKSRT
EEEEEEECCHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHC
KGYASLNYEEAGEQTADLVKVDILLQGEPVDAFSAIVHRDNAQWYGNKMTVKLKELIPRQ
CCCEECCHHHCCCHHHCEEEEEEEEECCCHHHHHHHHHCCCCCEECCEEEEEHHHHCCCH
QFEVPVQAAIGSKVIARENIRALRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKNIGSVE
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
VPQEAFVAALSTDEA
CCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 14602910 [H]