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The map label for this gene is truB [H]

Identifier: 62390819

GI number: 62390819

Start: 2058023

End: 2058916

Strand: Direct

Name: truB [H]

Synonym: cg2170

Alternate gene names: 62390819

Gene position: 2058023-2058916 (Clockwise)

Preceding gene: 62390814

Following gene: 62390829

Centisome position: 62.69

GC content: 59.4

Gene sequence:

>894_bases
ATGAATGCTCCTGCCCCTAAACCTGGACTCGTGATCGTCGACAAGCCCGCCGGAATGACATCCCATGACGTGGTGTCCAA
ATTGCGCCGCGCATTTTCCACCCGCAAAGTAGGCCACGCAGGCACCCTCGACCCCATGGCAACCGGCGTGTTAGTCGTCG
GAATTGAGCGCGGAACCCGCTTCCTGGCACACATGGTGGCCTCCACCAAAGCCTACGACGCCACCATTCGACTCGGCGCC
GCCACCAGCACCGATGATGCAGAAGGCGAGGTTATCTCCACAACAGACGCATCCGGCCTCGACCACAGCACCATCCTTGC
TGAAATCGTCAACCTCACCGGCGACATCATGCAAAAACCCACCAAAGTCTCCGCCATCAAAATCGACGGCAAACGCGCCC
ACGAACGCGTCCGCGACGGCGAAGAAGTAGACATTCCCGCACGTCCCGTCACCGTCAGCGTCTTTGACGTGCTCGACTAC
CACGTCGACGGTGAATTTTATGACTTAGATGTGCGCGTCCACTGCTCCTCCGGCACCTACATCCGCGCGCTCGCCCGCGA
CCTCGGCAACGCTTTGCAGGTCGGCGGCCACCTGACCGCGCTTAGGCGCACAGAGGTCGGCCCTTTTACGCTTAACGACG
CGACCCCCCTCTCCAAACTCCAAGAGAATCCAGAACTCTCCCTCAACCTCGACCAGGCACTCACCCGCAGTTACCCAGTC
CTTGACATCACCGAAGACGAAGGCGTTGACCTGTCCATGGGCAAATGGTTGGAACCTCGCGGACTGAAAGGCGTCCACGC
TGCAGTAACACCATCAGGAAAAGCCGTGGCGCTCATCGAAGAAAAGGGCAAACGCCTGGCCACCGTGTTTGTTGCTCACC
CCAACACTCTTTAG

Upstream 100 bases:

>100_bases
CACACCAATGGTGACTACTGATCCTTGAAGATCAGCCGGAACGCTGTCTAGTCCACTCCAAATATCCACTGTTTTAGACT
ACGGCATAGACTCAACAGAC

Downstream 100 bases:

>100_bases
TTGGTCTGCCAGAAGCCGATTTAAGAGGTCGAATTTCGCTAGATAGGTGTGAGTGTGGAAAGAGCGGTTTGGAGGGCTTA
AACGCGATTCTGAGAGGGCA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTRFLAHMVASTKAYDATIRLGA
ATSTDDAEGEVISTTDASGLDHSTILAEIVNLTGDIMQKPTKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDY
HVDGEFYDLDVRVHCSSGTYIRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL

Sequences:

>Translated_297_residues
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTRFLAHMVASTKAYDATIRLGA
ATSTDDAEGEVISTTDASGLDHSTILAEIVNLTGDIMQKPTKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDY
HVDGEFYDLDVRVHCSSGTYIRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL
>Mature_297_residues
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTRFLAHMVASTKAYDATIRLGA
ATSTDDAEGEVISTTDASGLDHSTILAEIVNLTGDIMQKPTKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDY
HVDGEFYDLDVRVHCSSGTYIRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=37.8378378378378, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI4503337, Length=210, Percent_Identity=32.8571428571429, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI215599015, Length=210, Percent_Identity=32.8571428571429, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI2367200, Length=214, Percent_Identity=36.4485981308411, Blast_Score=150, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323204, Length=205, Percent_Identity=32.6829268292683, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=187, Percent_Identity=29.4117647058824, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364189, Length=205, Percent_Identity=32.6829268292683, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364187, Length=205, Percent_Identity=32.6829268292683, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364185, Length=205, Percent_Identity=32.6829268292683, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364183, Length=205, Percent_Identity=32.6829268292683, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI62471759, Length=205, Percent_Identity=32.6829268292683, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17975520, Length=205, Percent_Identity=32.6829268292683, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR015225
- InterPro:   IPR014780 [H]

Pfam domain/function: PF09142 TruB_C; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 31787; Mature: 31787

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECHHHH
FLAHMVASTKAYDATIRLGAATSTDDAEGEVISTTDASGLDHSTILAEIVNLTGDIMQKP
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
TKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDYHVDGEFYDLDVRVHCSSGTY
CEEEEEEECCHHHHHHHCCCCCCCCCCCCEEEEEEEEEHHEECCEEEEEEEEEEECCCCH
IRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
HHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCHHHHHCCCCEEEEHHHHHHCCCCE
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL
EEECCCCCCEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEECCCCC
>Mature Secondary Structure
MNAPAPKPGLVIVDKPAGMTSHDVVSKLRRAFSTRKVGHAGTLDPMATGVLVVGIERGTR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCEEEEEECHHHH
FLAHMVASTKAYDATIRLGAATSTDDAEGEVISTTDASGLDHSTILAEIVNLTGDIMQKP
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
TKVSAIKIDGKRAHERVRDGEEVDIPARPVTVSVFDVLDYHVDGEFYDLDVRVHCSSGTY
CEEEEEEECCHHHHHHHCCCCCCCCCCCCEEEEEEEEEHHEECCEEEEEEEEEEECCCCH
IRALARDLGNALQVGGHLTALRRTEVGPFTLNDATPLSKLQENPELSLNLDQALTRSYPV
HHHHHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCHHHHHCCCCEEEEHHHHHHCCCCE
LDITEDEGVDLSMGKWLEPRGLKGVHAAVTPSGKAVALIEEKGKRLATVFVAHPNTL
EEECCCCCCEECCCCCCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA