Click here to switch to the map view.

The map label for this gene is topB [H]

Identifier: 62390723

GI number: 62390723

Start: 1954015

End: 1956294

Strand: Reverse

Name: topB [H]

Synonym: cg2064

Alternate gene names: 62390723

Gene position: 1956294-1954015 (Counterclockwise)

Preceding gene: 62390724

Following gene: 62390722

Centisome position: 59.59

GC content: 53.16

Gene sequence:

>2280_bases
TTGACAATTGGAATTTTGACGGAGAAAGCTTCGGCTGCGAAAAACTTTGCGAAAGCCCTCGGTGGACCCTCTGGCACTTA
TAACGGCGAGAAGTACGTTATTGCTGCAGCCAGCGGGCACCTTTATGAATTTGTCGAGCCAGAAGAGATGGTGCCACCAA
GCTATGCCGATAAGATCGGCGGCTACTGGGATCTGGCTAAGCTGCCGTGGGATGAGACGCAGTTTTTGTGGTCACGCGAG
CAAAAGCACGGTGCAGCTAACACGATTGCACGCATTAAGAAAACCTTGAGCAGCTGCACCACCATTGTGATCGGATCAGA
TATTGATCCCACGGGCGAAGGTGATCTGTTGGCGTGGGAGATTATCGCTGAGCTGGGGTTAGACACCAAGAAGATTCAGC
GTATGGAATTTGTTGATGAGGCGCCGGCCTCGATTCAGCAGGCGTTTATCAACCGCCGCGATGTGACCTCCATGCATGAC
GAGCCCAATTATCTTAAGGCGGAATTCCGATCACGCTTTGATTTGCTGTCGATGCAGTGGACTCGCGGTGCCACCAAAGT
GCTAGAGACCACTGGTCGGCGTGCAGTGTTGCGCAATGGTCGCCTGAAATCTGCCATGGTTGCGATTGTGGGGCAGGGCT
TAGATGCGTACAACGATTATAAAAAGATTCCTTTTTATCAAAACCGCTTCATTGATGATCACGGTGTAAGTTACGTCAAC
CCCGAGGAACCGCGTTATAAGACTTGCGACGAGGTACCACAGCTTTATAAGGCTAGTGCGGTCGAATGCTATGAGAAGAG
CATGAAAAAGACCGCGCCACCACGTCTACTTGACCTGGCGAGCCTGTCGGCCCTGCTGTCTAAAGAGGGCTTTAGCGCAA
AGAACGTGCTTAAGACTTATCAGCAGATGTACGAGGCGCAGGTCGTGTCTTATCCGCGTACCGAGGATAAGACGATTACC
CCTGAGCAGTTTAAGGAACTAGCGCCGTTGGTCGATAAGATCGCCGGCCTGGTTGGTGTGAACCCGGCTGACCTTACGCA
CCGACAGCCACGGTCCACGCATGTTAAGCCCAAGGGTGCACACGGCGCCAACCGTCCGGGTTTGAATGTGCCGACCAGTA
TTGCAGCGGTGAAAACCACCTACGGTGTGCTCGGACAGCGCATTTATGAAGTGCTCGCTAAGAGCTATCTCACGATGCTG
GCAGAGGATTACCTCTATGAGCACCAAAAAGGCCGCGTCGTGGATTACCCAGCGTTTCTCGGTACAGCAAATGTGCCAAA
GTCCTTGGGTTGGAAAGGTATTTTCGACGTAGACGCTGAGGCTGATGATGATGCTGCCGGCAGTGATGCAGCACAGCAGG
GGATTGGTACTCGTGCTGAGCCAGAAGTGTTTGAGGGTTTTCCGCAGCGACCACCGCATCCGTCAATGACGTGGTTGATG
AAACAGCTCGATAAGCATGATGTCGGTACCGGTGCGACACGAACGAGCACCTATGCCGAGGTCACAGCCGGCAAGTCAGC
ATTGCTCAAAGAAACGCGTGGCAAAGTCACTATGACTGACGCAGGGCAACTGAATTATCTGCTGCTTCCTGGCACCCACA
TTGGCGACCTCAAACTTACCGAGCGCGTTTATGCCGATATGGCAGCGGTTGCTGCTGGTGAAAAGACTGCAGAGCAGGCA
CTGGAACCAGTTAAACAGTGGGTCGAAGAAGATATTGCGACCATGACCCGTAACGCAAAAAACATCCCCCAAGAACTAGG
AAAGAAACTCATGACTACTACTTTTACCCCTTCTGTCAAGCACTCCGGCACCTGGAACGGTGAGCAGGTGAGCTTTAAGA
AGGTCTACTGCGGGCATGAATTTAGCGATGCAGAATGCGAGGCGTTGCTGCGCGGTGAGGAGCTCACCATTACCGATGCC
ATGATTGGTGGACAGATCACGACAGTCACCGGCAAGCTGGCGCATCAAAGCTTTAACAAAGACGGCAAGACCATTAAGTT
CTTTGGGTTTAAGGGGCAGGTTGATCGCATTGCCTCTGCAGACCCAGCTGTGTACGCGGTGGGTGTGTGGAAGGTTGAGG
GTGAAAAGATTCGCTTCAAGCGCGTATGGGGTGGTCACAAGTTCACCGACAAGGAGATCGCCGATCTGCTTGAGGGCCGC
GAGATTGCTTTTGATGCCATGTCGAAGGCCAAGAAGCCGTACACCGCTCGGGGATCGCTGCAGCGCGGCGAGTACAACGG
TAATTCGTTCGTCGGCTTCCAGCTCGCACCACGCGATTAA

Upstream 100 bases:

>100_bases
TGCGACCCGCATTGCTGGGTCGGTTGAATAACTCAAATAACTAATTCAATTAGTTTCTTGACCGTGTTAAAGTATTAAAA
AAGTAAGAAAGGTTAATACT

Downstream 100 bases:

>100_bases
CGCACAAGTACGTGCACTAGAAGAAACAAACCATTTGTTGAAGAAAGAAGGTGTGAGATGGCAAAAGCAGAGTACAAAGT
TCCACCGTCTCTAGCACGCA

Product: DNA topoisomerase I omega subunit

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 759; Mature: 758

Protein sequence:

>759_residues
MTIGILTEKASAAKNFAKALGGPSGTYNGEKYVIAAASGHLYEFVEPEEMVPPSYADKIGGYWDLAKLPWDETQFLWSRE
QKHGAANTIARIKKTLSSCTTIVIGSDIDPTGEGDLLAWEIIAELGLDTKKIQRMEFVDEAPASIQQAFINRRDVTSMHD
EPNYLKAEFRSRFDLLSMQWTRGATKVLETTGRRAVLRNGRLKSAMVAIVGQGLDAYNDYKKIPFYQNRFIDDHGVSYVN
PEEPRYKTCDEVPQLYKASAVECYEKSMKKTAPPRLLDLASLSALLSKEGFSAKNVLKTYQQMYEAQVVSYPRTEDKTIT
PEQFKELAPLVDKIAGLVGVNPADLTHRQPRSTHVKPKGAHGANRPGLNVPTSIAAVKTTYGVLGQRIYEVLAKSYLTML
AEDYLYEHQKGRVVDYPAFLGTANVPKSLGWKGIFDVDAEADDDAAGSDAAQQGIGTRAEPEVFEGFPQRPPHPSMTWLM
KQLDKHDVGTGATRTSTYAEVTAGKSALLKETRGKVTMTDAGQLNYLLLPGTHIGDLKLTERVYADMAAVAAGEKTAEQA
LEPVKQWVEEDIATMTRNAKNIPQELGKKLMTTTFTPSVKHSGTWNGEQVSFKKVYCGHEFSDAECEALLRGEELTITDA
MIGGQITTVTGKLAHQSFNKDGKTIKFFGFKGQVDRIASADPAVYAVGVWKVEGEKIRFKRVWGGHKFTDKEIADLLEGR
EIAFDAMSKAKKPYTARGSLQRGEYNGNSFVGFQLAPRD

Sequences:

>Translated_759_residues
MTIGILTEKASAAKNFAKALGGPSGTYNGEKYVIAAASGHLYEFVEPEEMVPPSYADKIGGYWDLAKLPWDETQFLWSRE
QKHGAANTIARIKKTLSSCTTIVIGSDIDPTGEGDLLAWEIIAELGLDTKKIQRMEFVDEAPASIQQAFINRRDVTSMHD
EPNYLKAEFRSRFDLLSMQWTRGATKVLETTGRRAVLRNGRLKSAMVAIVGQGLDAYNDYKKIPFYQNRFIDDHGVSYVN
PEEPRYKTCDEVPQLYKASAVECYEKSMKKTAPPRLLDLASLSALLSKEGFSAKNVLKTYQQMYEAQVVSYPRTEDKTIT
PEQFKELAPLVDKIAGLVGVNPADLTHRQPRSTHVKPKGAHGANRPGLNVPTSIAAVKTTYGVLGQRIYEVLAKSYLTML
AEDYLYEHQKGRVVDYPAFLGTANVPKSLGWKGIFDVDAEADDDAAGSDAAQQGIGTRAEPEVFEGFPQRPPHPSMTWLM
KQLDKHDVGTGATRTSTYAEVTAGKSALLKETRGKVTMTDAGQLNYLLLPGTHIGDLKLTERVYADMAAVAAGEKTAEQA
LEPVKQWVEEDIATMTRNAKNIPQELGKKLMTTTFTPSVKHSGTWNGEQVSFKKVYCGHEFSDAECEALLRGEELTITDA
MIGGQITTVTGKLAHQSFNKDGKTIKFFGFKGQVDRIASADPAVYAVGVWKVEGEKIRFKRVWGGHKFTDKEIADLLEGR
EIAFDAMSKAKKPYTARGSLQRGEYNGNSFVGFQLAPRD
>Mature_758_residues
TIGILTEKASAAKNFAKALGGPSGTYNGEKYVIAAASGHLYEFVEPEEMVPPSYADKIGGYWDLAKLPWDETQFLWSREQ
KHGAANTIARIKKTLSSCTTIVIGSDIDPTGEGDLLAWEIIAELGLDTKKIQRMEFVDEAPASIQQAFINRRDVTSMHDE
PNYLKAEFRSRFDLLSMQWTRGATKVLETTGRRAVLRNGRLKSAMVAIVGQGLDAYNDYKKIPFYQNRFIDDHGVSYVNP
EEPRYKTCDEVPQLYKASAVECYEKSMKKTAPPRLLDLASLSALLSKEGFSAKNVLKTYQQMYEAQVVSYPRTEDKTITP
EQFKELAPLVDKIAGLVGVNPADLTHRQPRSTHVKPKGAHGANRPGLNVPTSIAAVKTTYGVLGQRIYEVLAKSYLTMLA
EDYLYEHQKGRVVDYPAFLGTANVPKSLGWKGIFDVDAEADDDAAGSDAAQQGIGTRAEPEVFEGFPQRPPHPSMTWLMK
QLDKHDVGTGATRTSTYAEVTAGKSALLKETRGKVTMTDAGQLNYLLLPGTHIGDLKLTERVYADMAAVAAGEKTAEQAL
EPVKQWVEEDIATMTRNAKNIPQELGKKLMTTTFTPSVKHSGTWNGEQVSFKKVYCGHEFSDAECEALLRGEELTITDAM
IGGQITTVTGKLAHQSFNKDGKTIKFFGFKGQVDRIASADPAVYAVGVWKVEGEKIRFKRVWGGHKFTDKEIADLLEGRE
IAFDAMSKAKKPYTARGSLQRGEYNGNSFVGFQLAPRD

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1788061, Length=591, Percent_Identity=23.5194585448393, Blast_Score=77, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83839; Mature: 83708

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIGILTEKASAAKNFAKALGGPSGTYNGEKYVIAAASGHLYEFVEPEEMVPPSYADKIG
CEEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCHHCCCCCHHHHCC
GYWDLAKLPWDETQFLWSREQKHGAANTIARIKKTLSSCTTIVIGSDIDPTGEGDLLAWE
CEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHH
IIAELGLDTKKIQRMEFVDEAPASIQQAFINRRDVTSMHDEPNYLKAEFRSRFDLLSMQW
HHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
TRGATKVLETTGRRAVLRNGRLKSAMVAIVGQGLDAYNDYKKIPFYQNRFIDDHGVSYVN
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCCCCCCCC
PEEPRYKTCDEVPQLYKASAVECYEKSMKKTAPPRLLDLASLSALLSKEGFSAKNVLKTY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
QQMYEAQVVSYPRTEDKTITPEQFKELAPLVDKIAGLVGVNPADLTHRQPRSTHVKPKGA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCC
HGANRPGLNVPTSIAAVKTTYGVLGQRIYEVLAKSYLTMLAEDYLYEHQKGRVVDYPAFL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHC
GTANVPKSLGWKGIFDVDAEADDDAAGSDAAQQGIGTRAEPEVFEGFPQRPPHPSMTWLM
CCCCCCHHCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHH
KQLDKHDVGTGATRTSTYAEVTAGKSALLKETRGKVTMTDAGQLNYLLLPGTHIGDLKLT
HHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCHHHH
ERVYADMAAVAAGEKTAEQALEPVKQWVEEDIATMTRNAKNIPQELGKKLMTTTFTPSVK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
HSGTWNGEQVSFKKVYCGHEFSDAECEALLRGEELTITDAMIGGQITTVTGKLAHQSFNK
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHCCEEEEEEHHHHHHHCCC
DGKTIKFFGFKGQVDRIASADPAVYAVGVWKVEGEKIRFKRVWGGHKFTDKEIADLLEGR
CCCEEEEEECCCCHHHHCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCC
EIAFDAMSKAKKPYTARGSLQRGEYNGNSFVGFQLAPRD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure 
TIGILTEKASAAKNFAKALGGPSGTYNGEKYVIAAASGHLYEFVEPEEMVPPSYADKIG
EEEEECCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEECCCCHHCCCCCHHHHCC
GYWDLAKLPWDETQFLWSREQKHGAANTIARIKKTLSSCTTIVIGSDIDPTGEGDLLAWE
CEEECCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCEEEEECCCCCCCCCCCCHHHH
IIAELGLDTKKIQRMEFVDEAPASIQQAFINRRDVTSMHDEPNYLKAEFRSRFDLLSMQW
HHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
TRGATKVLETTGRRAVLRNGRLKSAMVAIVGQGLDAYNDYKKIPFYQNRFIDDHGVSYVN
HHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCHHHCCCCCCCCHHHCCCCCCCCCCCC
PEEPRYKTCDEVPQLYKASAVECYEKSMKKTAPPRLLDLASLSALLSKEGFSAKNVLKTY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
QQMYEAQVVSYPRTEDKTITPEQFKELAPLVDKIAGLVGVNPADLTHRQPRSTHVKPKGA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCC
HGANRPGLNVPTSIAAVKTTYGVLGQRIYEVLAKSYLTMLAEDYLYEHQKGRVVDYPAFL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHC
GTANVPKSLGWKGIFDVDAEADDDAAGSDAAQQGIGTRAEPEVFEGFPQRPPHPSMTWLM
CCCCCCHHCCCCCEEECCCCCCCCCCCCHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHH
KQLDKHDVGTGATRTSTYAEVTAGKSALLKETRGKVTMTDAGQLNYLLLPGTHIGDLKLT
HHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCHHHH
ERVYADMAAVAAGEKTAEQALEPVKQWVEEDIATMTRNAKNIPQELGKKLMTTTFTPSVK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
HSGTWNGEQVSFKKVYCGHEFSDAECEALLRGEELTITDAMIGGQITTVTGKLAHQSFNK
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCEEEEEHHHHCCEEEEEEHHHHHHHCCC
DGKTIKFFGFKGQVDRIASADPAVYAVGVWKVEGEKIRFKRVWGGHKFTDKEIADLLEGR
CCCEEEEEECCCCHHHHCCCCCCEEEEEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCC
EIAFDAMSKAKKPYTARGSLQRGEYNGNSFVGFQLAPRD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]