Click here to switch to the map view.

The map label for this gene is cglIM [H]

Identifier: 62390655

GI number: 62390655

Start: 1879784

End: 1880875

Strand: Direct

Name: cglIM [H]

Synonym: cg1996

Alternate gene names: 62390655

Gene position: 1879784-1880875 (Clockwise)

Preceding gene: 62390650

Following gene: 62390656

Centisome position: 57.26

GC content: 53.02

Gene sequence:

>1092_bases
ATGAGTACAAAACCCACTATTGTGTCCACATTTTCTGGTTGCGGTGGCCTCGATCTTGGCCTCCAAGAGGTCGGATTCGA
CCCCATTTGGGCCAACGACTTCTCAGAAGAAGCAGTCCAAACCTATAAGCACAACATCGGTGACCACATTGTTCACGGCG
ACATCACTGAAATTGATCCGTTTACTGATGACACCATCCCTGACGGCGATCTCGTCACCGGCGGCTTCCCGTGCCAGGAC
TTCTCCATGATCTGGAAGCGTCCTGGACTCGACGGCAAGCGTGGCACCCTGTACCAAAACTTCCGTGACTTTGTTGCAGC
AAAGAAGCCTAAAGCCTTTATCGCAGAAAACGTGAAGGGCCTACTCACTGCTAACCAGCACAAGGCTATTAAGACCATCA
TTGAAGACCTCGAAGCTGTTGAGCCTGGCTACATCGTCAAGCCTCGCCTGTACAACTTCGCTGAATACGGTGTCCCACAA
TTCCGCGAACGTGTGCTCATTGTTGGCATTCGCCGTGACACCGGCTTTGATTTCAAGCACCCAGCTCCTACCCATGGCCC
TCGCGGTGACATGCCGTATAAGACTGCCGGCGAAGCGCTCAAAGGCGTGAAGGATGTCCCCACAAACAACAACCACATGA
AGATCATGCCTCGCACCGTTGAAGTGCTTAAGCGCATCCCTGAGGGCGAAAACTTCACCGCGATCCCCAAAGATGACCCC
TACTACGTCAAGGGCATGATTAGTCACGTTTACCGTCGCTTGCACCGTGATGAGCCATCCAAAACCCTTATCGCCGGTGG
CGGCGGGGGTACATGGGGATACCATTATGAAGAAAATCGAGCATTGACCAACCGCGAGCGGGCTAGAATTCAATCGTTCC
CCGATGACTTTGAGTTTTTGGGATCAAACACCGAAGTCCGCCGCCAAATCGGTAATGCTGTTCCTCCTGTAGGTATGCAC
GCTGTGGGTGAGCGACTGATGAACCTGTACACCGGGAATTACACTCCCGTCGATCTAGAGGAACAGCACGCGTACCTGCA
GACGCTCTCCATTAAGGAACGTCTCGCGCTGGCTGATCAGGAAGCTGATTAA

Upstream 100 bases:

>100_bases
AGCGGCAAATATGTGCGGCTTGGCATAAACTGTGTCCATCGGTACCTGTGTAATAGACACACGGTAAACACGCACAAGAT
AAAACATTGCGAGATTTTTC

Downstream 100 bases:

>100_bases
GTAGATATATGAAGCCCACCGTTAATGTTGTGTTCAATGCGCATCACCCCAAAGATACGCAGCCGTTGGATAAGTTCTTC
GATAAAGAACTTAAAGACAC

Product: modification methylase

Products: NA

Alternate protein names: M.NgoFVII; Cytosine-specific methyltransferase NgoFVII; M.NgoVII [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSTKPTIVSTFSGCGGLDLGLQEVGFDPIWANDFSEEAVQTYKHNIGDHIVHGDITEIDPFTDDTIPDGDLVTGGFPCQD
FSMIWKRPGLDGKRGTLYQNFRDFVAAKKPKAFIAENVKGLLTANQHKAIKTIIEDLEAVEPGYIVKPRLYNFAEYGVPQ
FRERVLIVGIRRDTGFDFKHPAPTHGPRGDMPYKTAGEALKGVKDVPTNNNHMKIMPRTVEVLKRIPEGENFTAIPKDDP
YYVKGMISHVYRRLHRDEPSKTLIAGGGGGTWGYHYEENRALTNRERARIQSFPDDFEFLGSNTEVRRQIGNAVPPVGMH
AVGERLMNLYTGNYTPVDLEEQHAYLQTLSIKERLALADQEAD

Sequences:

>Translated_363_residues
MSTKPTIVSTFSGCGGLDLGLQEVGFDPIWANDFSEEAVQTYKHNIGDHIVHGDITEIDPFTDDTIPDGDLVTGGFPCQD
FSMIWKRPGLDGKRGTLYQNFRDFVAAKKPKAFIAENVKGLLTANQHKAIKTIIEDLEAVEPGYIVKPRLYNFAEYGVPQ
FRERVLIVGIRRDTGFDFKHPAPTHGPRGDMPYKTAGEALKGVKDVPTNNNHMKIMPRTVEVLKRIPEGENFTAIPKDDP
YYVKGMISHVYRRLHRDEPSKTLIAGGGGGTWGYHYEENRALTNRERARIQSFPDDFEFLGSNTEVRRQIGNAVPPVGMH
AVGERLMNLYTGNYTPVDLEEQHAYLQTLSIKERLALADQEAD
>Mature_362_residues
STKPTIVSTFSGCGGLDLGLQEVGFDPIWANDFSEEAVQTYKHNIGDHIVHGDITEIDPFTDDTIPDGDLVTGGFPCQDF
SMIWKRPGLDGKRGTLYQNFRDFVAAKKPKAFIAENVKGLLTANQHKAIKTIIEDLEAVEPGYIVKPRLYNFAEYGVPQF
RERVLIVGIRRDTGFDFKHPAPTHGPRGDMPYKTAGEALKGVKDVPTNNNHMKIMPRTVEVLKRIPEGENFTAIPKDDPY
YVKGMISHVYRRLHRDEPSKTLIAGGGGGTWGYHYEENRALTNRERARIQSFPDDFEFLGSNTEVRRQIGNAVPPVGMHA
VGERLMNLYTGNYTPVDLEEQHAYLQTLSIKERLALADQEAD

Specific function: This methylase recognizes the double-stranded sequence GCSGC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the NgoFVII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=202, Percent_Identity=29.2079207920792, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40706; Mature: 40575

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPTIVSTFSGCGGLDLGLQEVGFDPIWANDFSEEAVQTYKHNIGDHIVHGDITEIDP
CCCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCC
FTDDTIPDGDLVTGGFPCQDFSMIWKRPGLDGKRGTLYQNFRDFVAAKKPKAFIAENVKG
CCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHH
LLTANQHKAIKTIIEDLEAVEPGYIVKPRLYNFAEYGVPQFRERVLIVGIRRDTGFDFKH
HHCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHCCCEEEEEEECCCCCCCCC
PAPTHGPRGDMPYKTAGEALKGVKDVPTNNNHMKIMPRTVEVLKRIPEGENFTAIPKDDP
CCCCCCCCCCCCCHHHHHHHHCHHCCCCCCCEEEECHHHHHHHHHCCCCCCEEECCCCCC
YYVKGMISHVYRRLHRDEPSKTLIAGGGGGTWGYHYEENRALTNRERARIQSFPDDFEFL
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHH
GSNTEVRRQIGNAVPPVGMHAVGERLMNLYTGNYTPVDLEEQHAYLQTLSIKERLALADQ
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
EAD
CCC
>Mature Secondary Structure 
STKPTIVSTFSGCGGLDLGLQEVGFDPIWANDFSEEAVQTYKHNIGDHIVHGDITEIDP
CCCCCEEECCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCC
FTDDTIPDGDLVTGGFPCQDFSMIWKRPGLDGKRGTLYQNFRDFVAAKKPKAFIAENVKG
CCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHCHHH
LLTANQHKAIKTIIEDLEAVEPGYIVKPRLYNFAEYGVPQFRERVLIVGIRRDTGFDFKH
HHCCCHHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCHHHCCCEEEEEEECCCCCCCCC
PAPTHGPRGDMPYKTAGEALKGVKDVPTNNNHMKIMPRTVEVLKRIPEGENFTAIPKDDP
CCCCCCCCCCCCCHHHHHHHHCHHCCCCCCCEEEECHHHHHHHHHCCCCCCEEECCCCCC
YYVKGMISHVYRRLHRDEPSKTLIAGGGGGTWGYHYEENRALTNRERARIQSFPDDFEFL
HHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHH
GSNTEVRRQIGNAVPPVGMHAVGERLMNLYTGNYTPVDLEEQHAYLQTLSIKERLALADQ
CCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
EAD
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7607490 [H]