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The map label for this gene is polA [H]

Identifier: 62390237

GI number: 62390237

Start: 1415287

End: 1417929

Strand: Direct

Name: polA [H]

Synonym: cg1525

Alternate gene names: 62390237

Gene position: 1415287-1417929 (Clockwise)

Preceding gene: 62390235

Following gene: 62390239

Centisome position: 43.11

GC content: 55.43

Gene sequence:

>2643_bases
ATGCTTATCGACGGCCACTCGATGGCTTTCCGCGCATTCTTTGCTTTGCCGGCTGAGAATTTCTCCACGTCGGGCGGGCA
GGCCACCAATGCTGTCTATGGCTTTCTCTCGATGCTGTCCACGTTGTTGAAGGATGAGCAGCCTACTCATGTGGCGGTGG
CTTTCGATGTGGGGCGTAAGACGTTCCGTACCGATATGTTCCCGGCGTATAAGGCGCAGCGTGAAGCAACGCCACCTGAG
TTTAAGGGCCAGGTGGAAATCCTCAAGGAGGTGTTGTCCACTTTGGGAATTACGACTATTGAGAAAATCGATTTTGAGGC
TGATGATGTGATCGCCACGTTGTCTGTGGCGGCGAAACCTTTAGGCTTTAAGACGCTGATTGTTACGGGTGACCGTGATT
CCTTCCAGTTGGTCAATGACACCACCACGGTGTTGTATCCGATGAAGGGCGTGTCTGTGCTGCACCGTTTCACGCCGGAA
GCAGTGGAGGAGAAGTATGGACTGACACCGAGGCAGTATCCGGAGTTTGCAGCGCTGCGTGGTGATCCTTCCGATAACTT
GCCTAATATTCCTGGCGTGGGCGAGAAGACTGCTACCAAGTGGATTGCCCAGTATGAAACTTTGGATAATTTGCTTGATC
ACGCTGATGAGATCAAGGGCAAGGTTGGCGCCAGCCTGCGTGAGCGCATTGAGCAGGTCCGGATGAACCGCAAGCTCACG
GAGATGGTGAAGGATCTGGAGCTGCCGCTTGGTCCGGACGATTTTGAGATGAAGCCTGTGCAGGTTGCGGAGGTTGCGGC
GAAGTTTGACGATCTGGAGTTTGGTACCAATTTGCGTGAGCGGGTGCTGGCGGTGGTGAAGGCCGAGGGTTCCGCTGCCC
CCGTGGAGGAAGTGGAAGCGGAACAGGTTGTCGTCGATACGCAATCTTTGGCGCAATGGCTGCCTGCTAGGGCTGGCCAG
GCGCTTGCTTTAGCGCTGGCTGGAGTGGCTAAACCTGCTGCTGGCGACACGTATGCGCTAGCGATTGCGGATACCAAGCG
CCATGCGGTGTTGGTTGATGTGGCTGATATTTCAGCGGAGGATGAAAAGGCGCTGGCCACGTGGTTGGCGTCGGAAGATC
CAAAGATGCTGCACGGCGCTAAGGCCGCCTATCATATGCTCGCTGGGCGCGGTTTTGAGCTGCACGGCGTGGTGCATGAC
ACGGCGATCGCGGCATACTTGCTGCGTCCGGGCCAACGCACCTATGAGCTTGCCGACGTCTACCAGCGGCATCTTCAACG
ACAGTTGTCTACAAACGACAATGGCGGCCAGCTCACGCTGCTCGACGCAGCTGATGACCAATCGCTTGTTGATGATGTCA
TTGCAATCCTTGAGCTGTCTGAAGAATTGACCAAACAGCTTCAGGAGATTCAAGCTTTTGAGCTTTACCATGACCTGGAA
ATTCCGCTGTCGGGAATTCTGGCGCGCATGGAGGCCATCGGTATCGCTGTTGATGTTGCCACTTTGGAAGAGCAGTTGAA
GACTTTCATTGGTCAGGTTGCTCAGGAAGAGGAAGCAGCTCGCGAGCTCGCTGAGGATCCAACCCTGAATCTCTCGAGCC
CGAAGCAGCTGCAAGTGGTGCTTTTTGAGACGTTCGGAATGCCGAAAACCAAGAAAACCAAGACCGGCTACTCTACGGCT
GCCGCGGAAATTGAAGCCCTAGCGATCAAGAATCCGCACCCATTCCTAGATCACCTGTTGGCACACCGTCAGTACCAAAA
GATGAAGACCACTCTGGAAGGTCTCATCCGTGAGGTGGCTCCTGATGGCCGTATTCACACCACCTTCAACCAGACGGTGG
CGTCTACGGGACGTTTGTCATCCACTGATCCCAACCTGCAAAACATTCCTGTGCGCACTGAGGCTGGCCGAAAGATTCGT
TCGGGATTCGTCGTAGGCGAGGGGTATGAAACCTTGCTGACTGCCGACTATTCGCAGATTGAAATGCGCGTGATGGCTCA
CCTTTCCCAGGACCCAGGCTTGATTGAGGCGTACCGCGAAGGCGAAGACCTGCACAATTACGTGGGTTCCAAGGTGTTTA
ATGTGCCCATCGATGGCGTGACCCCTGAGCTGCGTCGCCAGGTCAAGGCCATGTCTTACGGTCTGGTGTACGGCTTGTCC
GCGTTTGGTTTGTCTCAGCAGCTGAGCATTCCTGCTGGCGAAGCGAAGCAGATCATGGAGTCCTACTTCGAGCGCTTCGG
CGGAGTACAGCGCTACCTCCGGGAGATCGTGGAGGAGGCTCGAAAAGCTGGCTACACGGAAACGCTGTTTGGGCGTCGTC
GCTACCTGCCGGAACTGACCTCGGATAACCGTGTCGCTCGTGAAAACGCTGAACGTGCCGCACTGAACGCCCCGATTCAG
GGAACTGCCGCAGACATCATCAAGGTGGCCATGATCCGGGTGGACCGTTCACTCAAGGAAGCTGCCGTGAAATCTCGCGT
GCTGCTTCAGGTGCATGATGAATTGGTCGTGGAAGTAGCGGCCGGTGAGTTGGAACAAGTCCGTGAGATTCTGGAACGCG
AAATGGATAACGCCATCAAGCTGTCCGTTCCTTTGGAAGTTTCAGCTGGTGATGGCGTTAACTGGGATGCTGCAGCGCAC
TAA

Upstream 100 bases:

>100_bases
TAGGAATGGAAATTAGGGGTCTGGTTTGGGTGAATGTGTCGCTAATTTTTCCACTCGCCTACACTCGGGAGGCGTGACTG
AGAAGACTGACCAGACCTTA

Downstream 100 bases:

>100_bases
GAGGTAACTGCCTTTTCGTCGACGAGCTGGGTTGGGCGCTCGCTACTCTTTCGGGTTTCGTGGCGCTTCAGCAGTGTGAC
CGCAAAGATGGTGGCCAGCG

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MLIDGHSMAFRAFFALPAENFSTSGGQATNAVYGFLSMLSTLLKDEQPTHVAVAFDVGRKTFRTDMFPAYKAQREATPPE
FKGQVEILKEVLSTLGITTIEKIDFEADDVIATLSVAAKPLGFKTLIVTGDRDSFQLVNDTTTVLYPMKGVSVLHRFTPE
AVEEKYGLTPRQYPEFAALRGDPSDNLPNIPGVGEKTATKWIAQYETLDNLLDHADEIKGKVGASLRERIEQVRMNRKLT
EMVKDLELPLGPDDFEMKPVQVAEVAAKFDDLEFGTNLRERVLAVVKAEGSAAPVEEVEAEQVVVDTQSLAQWLPARAGQ
ALALALAGVAKPAAGDTYALAIADTKRHAVLVDVADISAEDEKALATWLASEDPKMLHGAKAAYHMLAGRGFELHGVVHD
TAIAAYLLRPGQRTYELADVYQRHLQRQLSTNDNGGQLTLLDAADDQSLVDDVIAILELSEELTKQLQEIQAFELYHDLE
IPLSGILARMEAIGIAVDVATLEEQLKTFIGQVAQEEEAARELAEDPTLNLSSPKQLQVVLFETFGMPKTKKTKTGYSTA
AAEIEALAIKNPHPFLDHLLAHRQYQKMKTTLEGLIREVAPDGRIHTTFNQTVASTGRLSSTDPNLQNIPVRTEAGRKIR
SGFVVGEGYETLLTADYSQIEMRVMAHLSQDPGLIEAYREGEDLHNYVGSKVFNVPIDGVTPELRRQVKAMSYGLVYGLS
AFGLSQQLSIPAGEAKQIMESYFERFGGVQRYLREIVEEARKAGYTETLFGRRRYLPELTSDNRVARENAERAALNAPIQ
GTAADIIKVAMIRVDRSLKEAAVKSRVLLQVHDELVVEVAAGELEQVREILEREMDNAIKLSVPLEVSAGDGVNWDAAAH

Sequences:

>Translated_880_residues
MLIDGHSMAFRAFFALPAENFSTSGGQATNAVYGFLSMLSTLLKDEQPTHVAVAFDVGRKTFRTDMFPAYKAQREATPPE
FKGQVEILKEVLSTLGITTIEKIDFEADDVIATLSVAAKPLGFKTLIVTGDRDSFQLVNDTTTVLYPMKGVSVLHRFTPE
AVEEKYGLTPRQYPEFAALRGDPSDNLPNIPGVGEKTATKWIAQYETLDNLLDHADEIKGKVGASLRERIEQVRMNRKLT
EMVKDLELPLGPDDFEMKPVQVAEVAAKFDDLEFGTNLRERVLAVVKAEGSAAPVEEVEAEQVVVDTQSLAQWLPARAGQ
ALALALAGVAKPAAGDTYALAIADTKRHAVLVDVADISAEDEKALATWLASEDPKMLHGAKAAYHMLAGRGFELHGVVHD
TAIAAYLLRPGQRTYELADVYQRHLQRQLSTNDNGGQLTLLDAADDQSLVDDVIAILELSEELTKQLQEIQAFELYHDLE
IPLSGILARMEAIGIAVDVATLEEQLKTFIGQVAQEEEAARELAEDPTLNLSSPKQLQVVLFETFGMPKTKKTKTGYSTA
AAEIEALAIKNPHPFLDHLLAHRQYQKMKTTLEGLIREVAPDGRIHTTFNQTVASTGRLSSTDPNLQNIPVRTEAGRKIR
SGFVVGEGYETLLTADYSQIEMRVMAHLSQDPGLIEAYREGEDLHNYVGSKVFNVPIDGVTPELRRQVKAMSYGLVYGLS
AFGLSQQLSIPAGEAKQIMESYFERFGGVQRYLREIVEEARKAGYTETLFGRRRYLPELTSDNRVARENAERAALNAPIQ
GTAADIIKVAMIRVDRSLKEAAVKSRVLLQVHDELVVEVAAGELEQVREILEREMDNAIKLSVPLEVSAGDGVNWDAAAH
>Mature_880_residues
MLIDGHSMAFRAFFALPAENFSTSGGQATNAVYGFLSMLSTLLKDEQPTHVAVAFDVGRKTFRTDMFPAYKAQREATPPE
FKGQVEILKEVLSTLGITTIEKIDFEADDVIATLSVAAKPLGFKTLIVTGDRDSFQLVNDTTTVLYPMKGVSVLHRFTPE
AVEEKYGLTPRQYPEFAALRGDPSDNLPNIPGVGEKTATKWIAQYETLDNLLDHADEIKGKVGASLRERIEQVRMNRKLT
EMVKDLELPLGPDDFEMKPVQVAEVAAKFDDLEFGTNLRERVLAVVKAEGSAAPVEEVEAEQVVVDTQSLAQWLPARAGQ
ALALALAGVAKPAAGDTYALAIADTKRHAVLVDVADISAEDEKALATWLASEDPKMLHGAKAAYHMLAGRGFELHGVVHD
TAIAAYLLRPGQRTYELADVYQRHLQRQLSTNDNGGQLTLLDAADDQSLVDDVIAILELSEELTKQLQEIQAFELYHDLE
IPLSGILARMEAIGIAVDVATLEEQLKTFIGQVAQEEEAARELAEDPTLNLSSPKQLQVVLFETFGMPKTKKTKTGYSTA
AAEIEALAIKNPHPFLDHLLAHRQYQKMKTTLEGLIREVAPDGRIHTTFNQTVASTGRLSSTDPNLQNIPVRTEAGRKIR
SGFVVGEGYETLLTADYSQIEMRVMAHLSQDPGLIEAYREGEDLHNYVGSKVFNVPIDGVTPELRRQVKAMSYGLVYGLS
AFGLSQQLSIPAGEAKQIMESYFERFGGVQRYLREIVEEARKAGYTETLFGRRRYLPELTSDNRVARENAERAALNAPIQ
GTAADIIKVAMIRVDRSLKEAAVKSRVLLQVHDELVVEVAAGELEQVREILEREMDNAIKLSVPLEVSAGDGVNWDAAAH

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0749

COG function: function code L; DNA polymerase I - 3'-5' exonuclease and polymerase domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Homo sapiens, GI112420958, Length=507, Percent_Identity=29.1913214990138, Blast_Score=228, Evalue=2e-59,
Organism=Homo sapiens, GI139394648, Length=613, Percent_Identity=25.2854812398042, Blast_Score=185, Evalue=1e-46,
Organism=Escherichia coli, GI1790294, Length=928, Percent_Identity=36.3146551724138, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI226510970, Length=224, Percent_Identity=25.4464285714286, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI86563272, Length=388, Percent_Identity=31.4432989690722, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI17933644, Length=420, Percent_Identity=30.4761904761905, Blast_Score=202, Evalue=8e-52,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 96830; Mature: 96830

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00447 DNA_POLYMERASE_A

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIDGHSMAFRAFFALPAENFSTSGGQATNAVYGFLSMLSTLLKDEQPTHVAVAFDVGRK
CEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCH
TFRTDMFPAYKAQREATPPEFKGQVEILKEVLSTLGITTIEKIDFEADDVIATLSVAAKP
HHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCC
LGFKTLIVTGDRDSFQLVNDTTTVLYPMKGVSVLHRFTPEAVEEKYGLTPRQYPEFAALR
CCEEEEEEECCCCCEEEECCCEEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHCC
GDPSDNLPNIPGVGEKTATKWIAQYETLDNLLDHADEIKGKVGASLRERIEQVRMNRKLT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EMVKDLELPLGPDDFEMKPVQVAEVAAKFDDLEFGTNLRERVLAVVKAEGSAAPVEEVEA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHCH
EQVVVDTQSLAQWLPARAGQALALALAGVAKPAAGDTYALAIADTKRHAVLVDVADISAE
HHHEECHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCC
DEKALATWLASEDPKMLHGAKAAYHMLAGRGFELHGVVHDTAIAAYLLRPGQRTYELADV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHH
YQRHLQRQLSTNDNGGQLTLLDAADDQSLVDDVIAILELSEELTKQLQEIQAFELYHDLE
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPLSGILARMEAIGIAVDVATLEEQLKTFIGQVAQEEEAARELAEDPTLNLSSPKQLQVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
LFETFGMPKTKKTKTGYSTAAAEIEALAIKNPHPFLDHLLAHRQYQKMKTTLEGLIREVA
EEECCCCCCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDGRIHTTFNQTVASTGRLSSTDPNLQNIPVRTEAGRKIRSGFVVGEGYETLLTADYSQI
CCCCEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCHHHHHCCHHHH
EMRVMAHLSQDPGLIEAYREGEDLHNYVGSKVFNVPIDGVTPELRRQVKAMSYGLVYGLS
HHHHHHHCCCCCCHHHHHHCCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
AFGLSQQLSIPAGEAKQIMESYFERFGGVQRYLREIVEEARKAGYTETLFGRRRYLPELT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
SDNRVARENAERAALNAPIQGTAADIIKVAMIRVDRSLKEAAVKSRVLLQVHDELVVEVA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGELEQVREILEREMDNAIKLSVPLEVSAGDGVNWDAAAH
HCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLIDGHSMAFRAFFALPAENFSTSGGQATNAVYGFLSMLSTLLKDEQPTHVAVAFDVGRK
CEECCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCH
TFRTDMFPAYKAQREATPPEFKGQVEILKEVLSTLGITTIEKIDFEADDVIATLSVAAKP
HHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHCCC
LGFKTLIVTGDRDSFQLVNDTTTVLYPMKGVSVLHRFTPEAVEEKYGLTPRQYPEFAALR
CCEEEEEEECCCCCEEEECCCEEEEEECHHHHHHHHCCHHHHHHHHCCCCCCCCCHHHCC
GDPSDNLPNIPGVGEKTATKWIAQYETLDNLLDHADEIKGKVGASLRERIEQVRMNRKLT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
EMVKDLELPLGPDDFEMKPVQVAEVAAKFDDLEFGTNLRERVLAVVKAEGSAAPVEEVEA
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHCH
EQVVVDTQSLAQWLPARAGQALALALAGVAKPAAGDTYALAIADTKRHAVLVDVADISAE
HHHEECHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEEECCCCCC
DEKALATWLASEDPKMLHGAKAAYHMLAGRGFELHGVVHDTAIAAYLLRPGQRTYELADV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCCHHHHHHHH
YQRHLQRQLSTNDNGGQLTLLDAADDQSLVDDVIAILELSEELTKQLQEIQAFELYHDLE
HHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPLSGILARMEAIGIAVDVATLEEQLKTFIGQVAQEEEAARELAEDPTLNLSSPKQLQVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
LFETFGMPKTKKTKTGYSTAAAEIEALAIKNPHPFLDHLLAHRQYQKMKTTLEGLIREVA
EEECCCCCCCCCCCCCCHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDGRIHTTFNQTVASTGRLSSTDPNLQNIPVRTEAGRKIRSGFVVGEGYETLLTADYSQI
CCCCEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEECCCCHHHHHCCHHHH
EMRVMAHLSQDPGLIEAYREGEDLHNYVGSKVFNVPIDGVTPELRRQVKAMSYGLVYGLS
HHHHHHHCCCCCCHHHHHHCCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
AFGLSQQLSIPAGEAKQIMESYFERFGGVQRYLREIVEEARKAGYTETLFGRRRYLPELT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
SDNRVARENAERAALNAPIQGTAADIIKVAMIRVDRSLKEAAVKSRVLLQVHDELVVEVA
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGELEQVREILEREMDNAIKLSVPLEVSAGDGVNWDAAAH
HCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7476188; 11234002 [H]