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The map label for this gene is leuB [H]

Identifier: 62390172

GI number: 62390172

Start: 1354956

End: 1355978

Strand: Direct

Name: leuB [H]

Synonym: cg1453

Alternate gene names: 62390172

Gene position: 1354956-1355978 (Clockwise)

Preceding gene: 62390171

Following gene: 62390174

Centisome position: 41.28

GC content: 57.18

Gene sequence:

>1023_bases
ATGAAACTTGCTGTTATTGGTGGAGATGGTATCGGCCCAGAGGTTACTGCAGAAGCCCTCAAGGTTCTAAACGCTGTCCG
CGACGACATCGAGACCACCGATTATGACCTTGGCGCACGCCGTTACCTCAAAAATGGCGAGCTGCTCACCGACGAGGATC
TGGCATCCCTGCGCGAGCATGACGCGATCCTTCTTGGCGCTATCGGTGCACCAGGTTCCGTCCCTCCAGGAATTCTCGAG
CGTGGTTTGCTGCTGAAGATGCGATTCGCACTGGATCACCACGTGAACCTGCGCCCATCCAAGCTGTACGACGGCGTGGA
GTCCCCACTGCGTAACCCAGGCAAGATTGATTTCGTTGTGGTCCGCGAAGGTACCGAAGGCGCCTACACTGGCAACGGTG
GAGCAATCCGCGTGGGAACCCCTCACGAGATTGCCAATGAAACCTCCGTGAACACTCGCTACGGCGCTGAGCGCGTTATT
CGCTACGCATTCGAGCTGGCACAGAGCCGCCGCAAGAAGCTCACCCTCGTGCACAAGACCAACGTCCTGGTTCACGGTGG
TGGCCTGTGGCAGCGCACCGTAGATGAGGTTGCAAAGGAATACCCAGAGGTAGCCGTCGATTACAACCACATCGATGCAG
CAACCATCTATCTGGTCACTGATCCTTCCCGCTTCGATGTGATTGTTACCGATAACCTCTTCGGCGACATCCTCACCGAT
GAGGCAGGCGCAGTCTCTGGCGGAATTGGCCTCGCAGCATCCGGCAACATCGATGCCACGGGCACCAACCCTTCCATGTT
CGAGCCAGTCCACGGCTCTGCACCAGATATCGCAGGCCAGGGAATCGCAGACCCAACGGCAGCAATCCTATCCGCTGCGA
TGCTGCTGCGTCACTTAGGTGATGAAGACAACGCAGTACGTATTGAAACAGCCATCGCAGCTGATGTGGCTGGCCGAGAT
AACTCTCAGCCGATTTCTACCACTGAGGTGGGAGACCGCATCGTCAAGGCGCTGCAAAGCTAA

Upstream 100 bases:

>100_bases
GCCCTGCATGATGGGGTAGTGGGGGTTGTTGGGCAGGTACGAGCTGTGATCAATCAGCTACACTAGTGAAGTCCATATAG
TGAGAAGGGAATCCCACAAC

Downstream 100 bases:

>100_bases
ATTTCAACGCCGACCCCCTTGCCTTTTATGGCGAGGGGTTTACTTTTCGGTATGGGTTGAATTGGTTGGTGTAAATACTT
GCTGGATTGATATCGGGGAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHDAILLGAIGAPGSVPPGILE
RGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVVREGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVI
RYAFELAQSRRKKLTLVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFGDILTD
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLGDEDNAVRIETAIAADVAGRD
NSQPISTTEVGDRIVKALQS

Sequences:

>Translated_340_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHDAILLGAIGAPGSVPPGILE
RGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVVREGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVI
RYAFELAQSRRKKLTLVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFGDILTD
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLGDEDNAVRIETAIAADVAGRD
NSQPISTTEVGDRIVKALQS
>Mature_340_residues
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREHDAILLGAIGAPGSVPPGILE
RGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVVVREGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVI
RYAFELAQSRRKKLTLVHKTNVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFGDILTD
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLGDEDNAVRIETAIAADVAGRD
NSQPISTTEVGDRIVKALQS

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=315, Percent_Identity=35.5555555555556, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI4758582, Length=328, Percent_Identity=31.7073170731707, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI28178838, Length=320, Percent_Identity=32.1875, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28178821, Length=312, Percent_Identity=29.4871794871795, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI28178816, Length=316, Percent_Identity=29.746835443038, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI28178819, Length=163, Percent_Identity=36.1963190184049, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI1788101, Length=356, Percent_Identity=43.5393258426966, Blast_Score=270, Evalue=8e-74,
Organism=Escherichia coli, GI87081683, Length=356, Percent_Identity=41.5730337078652, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1787381, Length=392, Percent_Identity=29.0816326530612, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71986051, Length=312, Percent_Identity=35.2564102564103, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17550882, Length=302, Percent_Identity=33.112582781457, Blast_Score=150, Evalue=6e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=355, Percent_Identity=31.5492957746479, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=29.5522388059701, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324709, Length=319, Percent_Identity=36.0501567398119, Blast_Score=178, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6319830, Length=364, Percent_Identity=34.8901098901099, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=32.378223495702, Blast_Score=144, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6324291, Length=359, Percent_Identity=32.3119777158774, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643270, Length=311, Percent_Identity=36.0128617363344, Blast_Score=185, Evalue=4e-47,
Organism=Drosophila melanogaster, GI24643268, Length=311, Percent_Identity=36.0128617363344, Blast_Score=184, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24661184, Length=342, Percent_Identity=33.0409356725146, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI281362242, Length=304, Percent_Identity=29.9342105263158, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648872, Length=304, Percent_Identity=29.9342105263158, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078633, Length=351, Percent_Identity=30.4843304843305, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24650122, Length=351, Percent_Identity=30.4843304843305, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078635, Length=351, Percent_Identity=30.4843304843305, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078637, Length=351, Percent_Identity=30.4843304843305, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078639, Length=346, Percent_Identity=30.9248554913295, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI20130355, Length=313, Percent_Identity=27.4760383386581, Blast_Score=114, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36145; Mature: 36145

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREH
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEECHHHHHHHHHC
DAILLGAIGAPGSVPPGILERGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVV
CEEEEEECCCCCCCCHHHHHCCHHEEEEEHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEE
VREGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVIRYAFELAQSRRKKLTLVHKT
EEECCCCCEECCCCEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEC
NVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFGDILTD
EEEEECCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCHHHHHCC
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEDNAVRIETAIAADVAGRDNSQPISTTEVGDRIVKALQS
CCCCEEEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLAVIGGDGIGPEVTAEALKVLNAVRDDIETTDYDLGARRYLKNGELLTDEDLASLREH
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCEECHHHHHHHHHC
DAILLGAIGAPGSVPPGILERGLLLKMRFALDHHVNLRPSKLYDGVESPLRNPGKIDFVV
CEEEEEECCCCCCCCHHHHHCCHHEEEEEHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEE
VREGTEGAYTGNGGAIRVGTPHEIANETSVNTRYGAERVIRYAFELAQSRRKKLTLVHKT
EEECCCCCEECCCCEEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEC
NVLVHGGGLWQRTVDEVAKEYPEVAVDYNHIDAATIYLVTDPSRFDVIVTDNLFGDILTD
EEEEECCCHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCCEEEEEECCCHHHHHCC
EAGAVSGGIGLAASGNIDATGTNPSMFEPVHGSAPDIAGQGIADPTAAILSAAMLLRHLG
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHC
DEDNAVRIETAIAADVAGRDNSQPISTTEVGDRIVKALQS
CCCCEEEEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA