Click here to switch to the map view.

The map label for this gene is ilvB

Identifier: 62390158

GI number: 62390158

Start: 1339600

End: 1341480

Strand: Direct

Name: ilvB

Synonym: cg1435

Alternate gene names: 62390158

Gene position: 1339600-1341480 (Clockwise)

Preceding gene: 62390153

Following gene: 62390159

Centisome position: 40.81

GC content: 57.15

Gene sequence:

>1881_bases
GTGAATGTGGCAGCTTCTCAACAGCCCACTCCCGCCACGGTTGCAAGCCGTGGTCGATCCGCCGCCCCTGAGCGGATGAC
AGGTGCAAAGGCAATTGTTCGATCGCTCGAGGAGCTTAACGCCGACATCGTGTTCGGTATTCCTGGTGGTGCGGTGCTAC
CGGTGTATGACCCGCTCTATTCCTCCACAAAGGTGCGCCACGTCTTGGTGCGCCACGAGCAGGGCGCAGGCCACGCAGCA
ACCGGCTACGCGCAGGTTACTGGACGCGTTGGCGTCTGCATTGCAACCTCTGGCCCAGGAGCAACCAACTTGGTTACCCC
AATCGCTGATGCAAACTTGGACTCCGTTCCCATGGTTGCCATCACCGGCCAGGTCGGAAGTGGCCTGCTGGGTACCGACG
CTTTCCAGGAAGCCGATATCCGCGGCATCACCATGCCAGTGACCAAGCACAACTTCATGGTCACCAACCCTAACGACATT
CCACAGGCATTGGCTGAGGCATTCCACCTCGCGATTACTGGTCGCCCTGGCCCTGTTCTGGTGGATATTCCTAAGGATGT
CCAGAACGCTGAATTGGATTTCGTCTGGCCACCAAAGATCGACCTGCCAGGCTACCGCCCAGTTTCAACACCACATGCTC
GCCAGATCGAGCAGGCAGTCAAGCTGATCGGTGAGGCCAAGAAGCCCGTCCTTTACGTTGGTGGTGGCGTAATCAAGGCT
GACGCACACGAAGAGCTTCGTGCGTTCGCTGAGTACACCGGCATCCCAGTTGTCACCACCTTGATGGCTTTGGGTACTTT
CCCAGAGTCTCACGAGCTGCACATGGGTATGCCAGGCATGCATGGCACTGTGTCCGCTGTTGGTGCACTGCAGCGCAGCG
ACCTGCTGATTGCTATCGGCTCCCGCTTTGATGACCGCGTCACCGGTGACGTTGACACCTTCGCGCCTGACGCCAAGATC
ATTCACGCCGACATTGATCCTGCCGAAATCGGCAAGATCAAGCAGGTTGAGGTTCCAATCGTGGGCGATGCCCGCGAAGT
TCTTGCTCGTCTGCTGGAAACCACCAAGGCAAGCAAGGCAGAGACCGAGGACATCTCCGAGTGGGTTGACTACCTCAAGG
GCCTCAAGGCACGTTTCCCGCGTGGCTACGACGAGCAGCCAGGCGATCTGCTGGCACCACAGTTTGTCATTGAAACCCTG
TCCAAGGAAGTTGGCCCCGACGCAATTTACTGCGCCGGCGTTGGCCAGCACCAAATGTGGGCAGCTCAGTTCGTTGACTT
TGAAAAGCCACGCACCTGGCTCAACTCCGGTGGACTGGGCACCATGGGCTACGCAGTTCCTGCGGCCCTTGGAGCAAAGG
CTGGCGCACCTGACAAGGAAGTCTGGGCTATCGACGGCGACGGCTGTTTCCAGATGACCAACCAGGAACTCACCACCGCC
GCAGTTGAAGGTTTCCCCATTAAGATCGCACTAATCAACAACGGAAACCTGGGCATGGTTCGCCAATGGCAGACCCTATT
CTATGAAGGACGGTACTCAAATACTAAACTTCGTAACCAGGGCGAGTACATGCCCGACTTTGTTACCCTTTCTGAGGGAC
TTGGCTGTGTTGCCATCCGCGTCACCAAAGCGGAGGAAGTACTGCCAGCCATCCAAAAGGCTCGAGAGATCAACGACCGC
CCAGTAGTCATCGACTTCATCGTCGGTGAAGACGCACAGGTATGGCCAATGGTGTCTGCTGGATCATCCAACTCCGATAT
CCAGTACGCACTCGGATTGCGCCCATTCTTTGATGGTGATGAATCTGCAGCAGAAGATCCTGCCGACATTCACGAAGCCG
TCAGCGACATTGATGCCGCCGTTGAATCGACCGAGGCATAA

Upstream 100 bases:

>100_bases
ACAATTTTAAATGAGGGCTTTGTCTTTAGGCTGAGTTGAAATCGGCTTGGCTTGGACGGGTCCTGTGAAAATCCTTATTT
AGTAAAGGAGCCAGAAAGTC

Downstream 100 bases:

>100_bases
GGAGAGACCCAAGATGGCTAATTCTGACGTCACCCGCCACATCCTGTCCGTACTCGTTCAGGACGTAGACGGAATCATTT
CCCGCGTATCAGGTATGTTC

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MNVAASQQPTPATVASRGRSAAPERMTGAKAIVRSLEELNADIVFGIPGGAVLPVYDPLYSSTKVRHVLVRHEQGAGHAA
TGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVAITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTNPNDI
PQALAEAFHLAITGRPGPVLVDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIGSRFDDRVTGDVDTFAPDAKI
IHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKAETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETL
SKEVGPDAIYCAGVGQHQMWAAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVTLSEGLGCVAIRVTKAEEVLPAIQKAREINDR
PVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGDESAAEDPADIHEAVSDIDAAVESTEA

Sequences:

>Translated_626_residues
MNVAASQQPTPATVASRGRSAAPERMTGAKAIVRSLEELNADIVFGIPGGAVLPVYDPLYSSTKVRHVLVRHEQGAGHAA
TGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVAITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTNPNDI
PQALAEAFHLAITGRPGPVLVDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIGSRFDDRVTGDVDTFAPDAKI
IHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKAETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETL
SKEVGPDAIYCAGVGQHQMWAAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVTLSEGLGCVAIRVTKAEEVLPAIQKAREINDR
PVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGDESAAEDPADIHEAVSDIDAAVESTEA
>Mature_626_residues
MNVAASQQPTPATVASRGRSAAPERMTGAKAIVRSLEELNADIVFGIPGGAVLPVYDPLYSSTKVRHVLVRHEQGAGHAA
TGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVAITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTNPNDI
PQALAEAFHLAITGRPGPVLVDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIGSRFDDRVTGDVDTFAPDAKI
IHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKAETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETL
SKEVGPDAIYCAGVGQHQMWAAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVTLSEGLGCVAIRVTKAEEVLPAIQKAREINDR
PVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGDESAAEDPADIHEAVSDIDAAVESTEA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=484, Percent_Identity=26.4462809917355, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI21361361, Length=591, Percent_Identity=24.5346869712352, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=45.7142857142857, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1790104, Length=577, Percent_Identity=44.5407279029463, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=38.6524822695035, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI1787096, Length=565, Percent_Identity=29.9115044247788, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1788716, Length=555, Percent_Identity=27.7477477477477, Blast_Score=177, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17531301, Length=577, Percent_Identity=26.3431542461005, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=577, Percent_Identity=26.3431542461005, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17542570, Length=511, Percent_Identity=26.8101761252446, Blast_Score=135, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=609, Percent_Identity=43.8423645320197, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=576, Percent_Identity=23.7847222222222, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=494, Percent_Identity=24.8987854251012, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323073, Length=503, Percent_Identity=24.4532803180915, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922626, Length=552, Percent_Identity=25.5434782608696, Blast_Score=164, Evalue=2e-40,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVB_CORGL (P42463)

Other databases:

- EMBL:   L09232
- EMBL:   BA000036
- EMBL:   BX927151
- PIR:   A48648
- RefSeq:   NP_600493.1
- RefSeq:   YP_225560.1
- ProteinModelPortal:   P42463
- SMR:   P42463
- World-2DPAGE:   0001:P42463
- PRIDE:   P42463
- GeneID:   1019252
- GeneID:   3345149
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1435
- KEGG:   cgl:NCgl1222
- HOGENOM:   HBG323037
- OMA:   EVINCIN
- PhylomeDB:   P42463
- ProtClustDB:   PRK07789
- BioCyc:   CGLU196627:CG1435-MONOMER
- BRENDA:   2.2.1.6
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 66846; Mature: 66846

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 73-73 BINDING 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAASQQPTPATVASRGRSAAPERMTGAKAIVRSLEELNADIVFGIPGGAVLPVYDPLY
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCC
SSTKVRHVLVRHEQGAGHAATGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVA
CCCCEEEEEEEECCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
ITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTNPNDIPQALAEAFHLAITGRPGPVL
EECCCCCCCCCCCCHHCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHEEEEECCCCCEE
VDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
EECCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEC
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCCEEEEEC
SRFDDRVTGDVDTFAPDAKIIHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKA
CCCCCCCCCCHHHCCCCCEEEEECCCHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCC
ETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETLSKEVGPDAIYCAGVGQHQMW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
AAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
HHHHHCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHH
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVTLSEGLGCVAIR
HCCCCEEEEEEEECCCCCHHHHEEHHEECCCCCCCEECCCCCCCCCEEECCCCCCEEEEE
VTKAEEVLPAIQKAREINDRPVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGD
EECHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEEECCCCCCCCC
ESAAEDPADIHEAVSDIDAAVESTEA
CCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNVAASQQPTPATVASRGRSAAPERMTGAKAIVRSLEELNADIVFGIPGGAVLPVYDPLY
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCC
SSTKVRHVLVRHEQGAGHAATGYAQVTGRVGVCIATSGPGATNLVTPIADANLDSVPMVA
CCCCEEEEEEEECCCCCCCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEE
ITGQVGSGLLGTDAFQEADIRGITMPVTKHNFMVTNPNDIPQALAEAFHLAITGRPGPVL
EECCCCCCCCCCCCHHCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHEEEEECCCCCEE
VDIPKDVQNAELDFVWPPKIDLPGYRPVSTPHARQIEQAVKLIGEAKKPVLYVGGGVIKA
EECCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCEEEC
DAHEELRAFAEYTGIPVVTTLMALGTFPESHELHMGMPGMHGTVSAVGALQRSDLLIAIG
CCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCEECCCCCCCHHHHHHHHHHHCCEEEEEC
SRFDDRVTGDVDTFAPDAKIIHADIDPAEIGKIKQVEVPIVGDAREVLARLLETTKASKA
CCCCCCCCCCHHHCCCCCEEEEECCCHHHCCCEEEEEEEECCCHHHHHHHHHHHHCCCCC
ETEDISEWVDYLKGLKARFPRGYDEQPGDLLAPQFVIETLSKEVGPDAIYCAGVGQHQMW
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHH
AAQFVDFEKPRTWLNSGGLGTMGYAVPAALGAKAGAPDKEVWAIDGDGCFQMTNQELTTA
HHHHHCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEECCCHHHHH
AVEGFPIKIALINNGNLGMVRQWQTLFYEGRYSNTKLRNQGEYMPDFVTLSEGLGCVAIR
HCCCCEEEEEEEECCCCCHHHHEEHHEECCCCCCCEECCCCCCCCCEEECCCCCCEEEEE
VTKAEEVLPAIQKAREINDRPVVIDFIVGEDAQVWPMVSAGSSNSDIQYALGLRPFFDGD
EECHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEEECCCCCCCCC
ESAAEDPADIHEAVSDIDAAVESTEA
CCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8366043; 12948626