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The map label for this gene is gatB [H]

Identifier: 62390146

GI number: 62390146

Start: 1326223

End: 1327728

Strand: Direct

Name: gatB [H]

Synonym: cg1420

Alternate gene names: 62390146

Gene position: 1326223-1327728 (Clockwise)

Preceding gene: 62390145

Following gene: 62390147

Centisome position: 40.4

GC content: 56.37

Gene sequence:

>1506_bases
ATGACTGCAGCCTTTTATGATCTGATGGACTTCGATGAAGTTCTAGAGAAATATGACCCGGTGATGGGACTTGAGGTCCA
CGTCGAACTGGGCACTGAGACCAAGATGTTCTCTGCATCTTCTGCACACTTTGGCGCTGAACCCAATAGCAATGTTGACC
CAGTTTCTTTGGGTCTCCCAGGCGCACTTCCTGTGGTTAACGCCAAGGGCGTGGAGTGGGCAATTAAGATTGGACTGGCG
CTGAACTGCAGCATCGCTGAGTCCTCACGCTTTGCACGTAAGAACTACTTCTACCCGGATCAGCCTAAGAACTACCAGAT
CTCCCAGTATGACGAGCCAATCGCATACGACGGCTACCTGGATGTTGTTCTGGAGGACGGCACCGAGTGGCGTGTGGAAA
TCGAACGCGCTCACATGGAGGAAGACACCGGAAAGCTCACCCACCTGGGTGGTACTTCTGGTCGTATTCACGGCGCAACC
GCTTCTTTGGTGGACTGCAACCGTGCAGGCGTCCCTTTGATTGAGGTTGTCACCAAGCCAATCGAAGGCGCTGGCGCTCG
CGCTCCAGAGATCGCTAAGGCTTATGTCTCCGCACTGCGCGATCTGGTTAAGGCGCTCGGTGTGTCCGACGGCCGCTTGG
ATCAGGGTTCTATGCGTGTCGACGCCAACCTGTCCCTGCGCCCGATCGGCCAGGAGGAATTCGGCACCCGTACCGAAACC
AAGAACATCAACTCCCTGAAGTCCGTTGAGCAGGCCATCACCTTTGAGATGCAGCGCCAGGCTCAGGTACTGGACGACGG
TGGTGTGATTGATCAGGAAACCCGCCACTACCAGGAGGCAGACGGAAGTACCTCCAAGGGTCGTCCAAAGGAAACCGCTG
AGGATTACCGTTACTTCAACGATCCTGACCTGCCTCCAGTGATTGCGCCTCGTGAGTGGGTGGAAGAAATCCGCGCAACT
CTTCCAGAGCTGCCTTGGGTTCGCCGTGCACGTATCCAGGAAGAGTGGAAGCTTTCCGACGCCGAGATGCGCGACCTCAT
CAACGCCAACGCGCTCGACCTCATCATCGAGACCGTGGAAGCGGGTACCACTCCTGATGAAGCTCGTGCTTGGTGGGTTT
CTTACATCTCTCAGAAGGCTAACGAGTCTGGTGTCGAGCTCGATGCTCTCGGTGTTGCGCCAGCTCACGTTGCTCGCGTC
GTTGCGCTTGTTTCTGAAGGCAAGCTGACCAATAAGCTGGCACGTCAGGCTATCGACGGCGTTATTGCTGGCGAAGGCGA
TGTGGACGCGGTTGTTGCTGCTCGTGGACTGGAAGTTGTTCGCGATGACGGCGCAATTGAAAAGGCTGTCGATGATGCCT
TGGCTGCAAACCCAGACATCGTGGAGAAGTACCGCGCTGGCAACACCAAGGTCACCGGCGCCATCGTTGGTGCTGTCATG
AAGGCGACCCGCGGTAAGGCTGACCCAGCTCAGGTGAACCAGCTGATCGCAAAGAAGTTGGCTTAA

Upstream 100 bases:

>100_bases
CTCAATTAAGCAGCAATCCGAGCCGGGGGAGCGCTGTCAGAAATGGACCGTGCCACCCCATGACAACATGCTTGCACAAT
GATGACTAGAATAATGACCC

Downstream 100 bases:

>100_bases
GCTTTTTGCCGTTGAGCTGCTAAAAGTCTCGCATTAAAGGACTGCTTCCCTTCGGGGGAGCATTTCTTTTTAACTAATTA
CTTGACACGTCAAGTAATTA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTAAFYDLMDFDEVLEKYDPVMGLEVHVELGTETKMFSASSAHFGAEPNSNVDPVSLGLPGALPVVNAKGVEWAIKIGLA
LNCSIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYLDVVLEDGTEWRVEIERAHMEEDTGKLTHLGGTSGRIHGAT
ASLVDCNRAGVPLIEVVTKPIEGAGARAPEIAKAYVSALRDLVKALGVSDGRLDQGSMRVDANLSLRPIGQEEFGTRTET
KNINSLKSVEQAITFEMQRQAQVLDDGGVIDQETRHYQEADGSTSKGRPKETAEDYRYFNDPDLPPVIAPREWVEEIRAT
LPELPWVRRARIQEEWKLSDAEMRDLINANALDLIIETVEAGTTPDEARAWWVSYISQKANESGVELDALGVAPAHVARV
VALVSEGKLTNKLARQAIDGVIAGEGDVDAVVAARGLEVVRDDGAIEKAVDDALAANPDIVEKYRAGNTKVTGAIVGAVM
KATRGKADPAQVNQLIAKKLA

Sequences:

>Translated_501_residues
MTAAFYDLMDFDEVLEKYDPVMGLEVHVELGTETKMFSASSAHFGAEPNSNVDPVSLGLPGALPVVNAKGVEWAIKIGLA
LNCSIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYLDVVLEDGTEWRVEIERAHMEEDTGKLTHLGGTSGRIHGAT
ASLVDCNRAGVPLIEVVTKPIEGAGARAPEIAKAYVSALRDLVKALGVSDGRLDQGSMRVDANLSLRPIGQEEFGTRTET
KNINSLKSVEQAITFEMQRQAQVLDDGGVIDQETRHYQEADGSTSKGRPKETAEDYRYFNDPDLPPVIAPREWVEEIRAT
LPELPWVRRARIQEEWKLSDAEMRDLINANALDLIIETVEAGTTPDEARAWWVSYISQKANESGVELDALGVAPAHVARV
VALVSEGKLTNKLARQAIDGVIAGEGDVDAVVAARGLEVVRDDGAIEKAVDDALAANPDIVEKYRAGNTKVTGAIVGAVM
KATRGKADPAQVNQLIAKKLA
>Mature_500_residues
TAAFYDLMDFDEVLEKYDPVMGLEVHVELGTETKMFSASSAHFGAEPNSNVDPVSLGLPGALPVVNAKGVEWAIKIGLAL
NCSIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYLDVVLEDGTEWRVEIERAHMEEDTGKLTHLGGTSGRIHGATA
SLVDCNRAGVPLIEVVTKPIEGAGARAPEIAKAYVSALRDLVKALGVSDGRLDQGSMRVDANLSLRPIGQEEFGTRTETK
NINSLKSVEQAITFEMQRQAQVLDDGGVIDQETRHYQEADGSTSKGRPKETAEDYRYFNDPDLPPVIAPREWVEEIRATL
PELPWVRRARIQEEWKLSDAEMRDLINANALDLIIETVEAGTTPDEARAWWVSYISQKANESGVELDALGVAPAHVARVV
ALVSEGKLTNKLARQAIDGVIAGEGDVDAVVAARGLEVVRDDGAIEKAVDDALAANPDIVEKYRAGNTKVTGAIVGAVMK
ATRGKADPAQVNQLIAKKLA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=508, Percent_Identity=33.6614173228346, Blast_Score=279, Evalue=5e-75,
Organism=Caenorhabditis elegans, GI115534094, Length=312, Percent_Identity=38.7820512820513, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6319391, Length=344, Percent_Identity=34.8837209302326, Blast_Score=189, Evalue=9e-49,
Organism=Drosophila melanogaster, GI45550808, Length=502, Percent_Identity=31.6733067729084, Blast_Score=229, Evalue=5e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 54466; Mature: 54335

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAFYDLMDFDEVLEKYDPVMGLEVHVELGTETKMFSASSAHFGAEPNSNVDPVSLGLP
CCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEECCC
GALPVVNAKGVEWAIKIGLALNCSIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYL
CCCCEECCCCCEEEEEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEE
DVVLEDGTEWRVEIERAHMEEDTGKLTHLGGTSGRIHGATASLVDCNRAGVPLIEVVTKP
EEEEECCCCEEEEEHHHHCCCCCCCEEECCCCCCEEECCEEHEEECCCCCCHHHHHHHCC
IEGAGARAPEIAKAYVSALRDLVKALGVSDGRLDQGSMRVDANLSLRPIGQEEFGTRTET
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEECCHHHCCCCCCC
KNINSLKSVEQAITFEMQRQAQVLDDGGVIDQETRHYQEADGSTSKGRPKETAEDYRYFN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
DPDLPPVIAPREWVEEIRATLPELPWVRRARIQEEWKLSDAEMRDLINANALDLIIETVE
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHH
AGTTPDEARAWWVSYISQKANESGVELDALGVAPAHVARVVALVSEGKLTNKLARQAIDG
CCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCC
VIAGEGDVDAVVAARGLEVVRDDGAIEKAVDDALAANPDIVEKYRAGNTKVTGAIVGAVM
EECCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHHHH
KATRGKADPAQVNQLIAKKLA
HHHCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TAAFYDLMDFDEVLEKYDPVMGLEVHVELGTETKMFSASSAHFGAEPNSNVDPVSLGLP
CCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCCCCCCCCEEEECCC
GALPVVNAKGVEWAIKIGLALNCSIAESSRFARKNYFYPDQPKNYQISQYDEPIAYDGYL
CCCCEECCCCCEEEEEEEEEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCEE
DVVLEDGTEWRVEIERAHMEEDTGKLTHLGGTSGRIHGATASLVDCNRAGVPLIEVVTKP
EEEEECCCCEEEEEHHHHCCCCCCCEEECCCCCCEEECCEEHEEECCCCCCHHHHHHHCC
IEGAGARAPEIAKAYVSALRDLVKALGVSDGRLDQGSMRVDANLSLRPIGQEEFGTRTET
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEECCHHHCCCCCCC
KNINSLKSVEQAITFEMQRQAQVLDDGGVIDQETRHYQEADGSTSKGRPKETAEDYRYFN
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHCCCCC
DPDLPPVIAPREWVEEIRATLPELPWVRRARIQEEWKLSDAEMRDLINANALDLIIETVE
CCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHH
AGTTPDEARAWWVSYISQKANESGVELDALGVAPAHVARVVALVSEGKLTNKLARQAIDG
CCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHCC
VIAGEGDVDAVVAARGLEVVRDDGAIEKAVDDALAANPDIVEKYRAGNTKVTGAIVGAVM
EECCCCCHHHHHHHHCHHHHHCCCHHHHHHHHHHCCCCHHHHHHHCCCCEEHHHHHHHHH
KATRGKADPAQVNQLIAKKLA
HHHCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]