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The map label for this gene is ligA [H]

Identifier: 62390131

GI number: 62390131

Start: 1309798

End: 1311840

Strand: Direct

Name: ligA [H]

Synonym: cg1401

Alternate gene names: 62390131

Gene position: 1309798-1311840 (Clockwise)

Preceding gene: 62390128

Following gene: 62390133

Centisome position: 39.9

GC content: 55.95

Gene sequence:

>2043_bases
GTGACTGAAGATAATGCTCAACTGCGTAGAACGTGGAACGACTTAGCCGAGAAGGTTCGTTATCACCGAGATCGTTATTA
CAACGAACAGCCAGAGATCCCTGATGCTGATTTTGATGCGCTTTTTAAGCAGCTTCAGCAGTTGGAAGAAGACCACCCGG
AGCTCGCCGTCCCTGATAGCCCCACCATGGTTGTGGGCGCTCCGGTGGCAGAGCAATCAAGCTTTGACAATGTTGAGCAC
TTGGAGCGAATGCTCAGCTTGGACAATGTTTTTGATGAGCAGGAGTTGCGTGATTGGTTGGGCAGGACGCCAGCCAAGCA
GTATTTGACGGAGTTGAAAATTGATGGCTTGTCCATCGACTTGGTGTATCGCAATGGCCAGTTAGAGCGTGCCGCTACTC
GTGGTGATGGTCGCGTGGGCGAGGACATCACGGCCAATGCTCGCGTGATCGAAGATATCCCGCACCAGCTTCAGGGCACT
GATGAATATCCTGTGCCTGCTGTGCTGGAAATTCGCGGTGAGGTGTTCATCACTGTGGAGGATTTCCCAGAGGTCAACGC
GCAGCGCATTGCTGATGGTGGCAAGCCGTTTGCCAACCCGCGTAATGCTGCGGCTGGTTCTCTGCGTCAGAAAAATATTG
AGGACGTGAAGAAGCGCCGCCTGCGGATGATCAGCCATGGCATCGGTTTCACTGAAGGCTTTAGCCCTGCGTCTCAGCAT
GATGCGTATCTGGCATTGGCTGCCTGGGGTTTGCCCACCTCGCCGTACACAGAGGCTGTGACTGATCCAGAAGATGTGGT
GAAAAAGGTCAGCTACTGGGCTGATCACCGCCACGACGCACTCCATGAGATGGATGGCCTGGTGATTAAGGTCGATGACA
TCGCATCTCAGCGTGCTTTGGGTTCCACCAGCCGCGCGCCTCGCTGGGCCATTGCGTACAAGTACCCTCCGGAGGAGGTC
ACCACCAAGCTGCTTGATATTCAGGTTGGCGTTGGTCGCACCGGCCGTGTCACCCCATTCGCGGTCATGGAGCCGGTTCT
TGTTGCAGGATCAACGGTGTCTATGGCGACGCTGCATAACCAGAGCGAAGTCAAGCGTAAAGGCGTGCTCATCGGTGACA
CCGTGGTTATCCGCAAGGCGGGCGAGGTTATCCCAGAGGTGCTTGGCCCTGTCGTAGAGCTTCGTGACGGCACAGAGCGC
GAGTACATCTTCCCAACGCTGTGCCCTGAATGCGGTACCCGTCTGGCGCCCGCGAAGGCCGATGACGTGGATTGGCGTTG
CCCCAACATGCAAAGCTGTCCAGGTCAGCTGTCCACGCGTTTGACCTACCTTGCTGGTCGTGGCGCTTTTGATATTGAAG
CATTGGGCGAAAAGGGCGCTGAAGACCTCATTCGCACCGGCATTTTGCTTGACGAGTCTGGCCTGTTCGACCTCACAGAG
GACGATCTGCTGAGCTCCAATGTCTACACCACCAACGCCGGCAAAGTAAATGCCAGCGGCAAGAAACTGCTGGACAACCT
GCAAAAATCCAAGCAGACCGACCTCTGGCGAGTCCTCGTGGCATTATCTATCAGGCACGTAGGCCCCACCGCAGCGCGCG
CCCTTGCAGGTCGCTACCACTCCATCCAGGCGCTTATCGACGCCCCCCTCGAGGAACTCTCCGAAACCGATGGAGTAGGT
ACCATCATTGCCCAATCCTTCAAGGACTGGTTCGAGGTTGATTGGCACAAGGCCATCGTGGACAAGTGGGCAGCCGCTGG
TGTGACTATGGAGGAAGAAGTAGGGGAGGTCGCTGAACAAACCCTTGAAGGCCTAACCATCGTGGTCACCGGAGGGTTGG
AAGGCTTCACCAGAGATTCGGTGAAGGAAGCCATCATCTCCCGTGGCGGAAAAGCCTCTGGATCTGTCTCGAAGAAAACT
GACTACGTGGTGATTGGTGAAAACGCAGGTTCCAAGGCCACCAAGGCAGAAGAACTAGGGCTGCGCATTCTGGATGAGGC
AGGATTCGTCCGTTTGCTCAATACCGGCTCAGCTGACGAATAG

Upstream 100 bases:

>100_bases
AGGTCTGCACCCATTCATACGCGAATTTTGACGTGAGTTTTAGCTTTCCGAGCACCTGTGCGAATGGCCGTGTCCACCCT
TAGGGTTAGAATCGGGAACC

Downstream 100 bases:

>100_bases
TCGCACATGAAAATGCCCCAAGGAACATCTTCCTTGGGGCATTTTTTAAACAGCTCAGATTATTTTTGAAGCATTGCTCC
GATAATGTCACCGACTTGGC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 680; Mature: 679

Protein sequence:

>680_residues
MTEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDSPTMVVGAPVAEQSSFDNVEH
LERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSIDLVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGT
DEYPVPAVLEIRGEVFITVEDFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRALGSTSRAPRWAIAYKYPPEEV
TTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHNQSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTER
EYIFPTLCPECGTRLAPAKADDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYHSIQALIDAPLEELSETDGVG
TIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQTLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKT
DYVVIGENAGSKATKAEELGLRILDEAGFVRLLNTGSADE

Sequences:

>Translated_680_residues
MTEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDSPTMVVGAPVAEQSSFDNVEH
LERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSIDLVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGT
DEYPVPAVLEIRGEVFITVEDFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRALGSTSRAPRWAIAYKYPPEEV
TTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHNQSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTER
EYIFPTLCPECGTRLAPAKADDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYHSIQALIDAPLEELSETDGVG
TIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQTLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKT
DYVVIGENAGSKATKAEELGLRILDEAGFVRLLNTGSADE
>Mature_679_residues
TEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDSPTMVVGAPVAEQSSFDNVEHL
ERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSIDLVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGTD
EYPVPAVLEIRGEVFITVEDFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQHD
AYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRALGSTSRAPRWAIAYKYPPEEVT
TKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHNQSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTERE
YIFPTLCPECGTRLAPAKADDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTED
DLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYHSIQALIDAPLEELSETDGVGT
IIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQTLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKTD
YVVIGENAGSKATKAEELGLRILDEAGFVRLLNTGSADE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=85, Percent_Identity=48.2352941176471, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI1788750, Length=687, Percent_Identity=39.7379912663755, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI87082305, Length=457, Percent_Identity=24.2888402625821, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 74707; Mature: 74576

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDS
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCC
PTMVVGAPVAEQSSFDNVEHLERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSID
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHEECCEEEE
LVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGTDEYPVPAVLEIRGEVFITVE
EEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHCHHHHCCCCCCCCCEEEEECCCEEEEEC
DFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRAL
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHC
GSTSRAPRWAIAYKYPPEEVTTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHN
CCCCCCCCEEEEEECCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHCC
QSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTEREYIFPTLCPECGTRLAPAKA
HHHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCEECCHHCHHHCCCCCCCCC
DDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
CCCCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHCCEECCCCCCCCCH
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYH
HHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SIQALIDAPLEELSETDGVGTIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKTDYVVIGENAGSKATKAEELG
HHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHC
LRILDEAGFVRLLNTGSADE
CEEECCCCEEEEECCCCCCC
>Mature Secondary Structure 
TEDNAQLRRTWNDLAEKVRYHRDRYYNEQPEIPDADFDALFKQLQQLEEDHPELAVPDS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCC
PTMVVGAPVAEQSSFDNVEHLERMLSLDNVFDEQELRDWLGRTPAKQYLTELKIDGLSID
CCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHEECCEEEE
LVYRNGQLERAATRGDGRVGEDITANARVIEDIPHQLQGTDEYPVPAVLEIRGEVFITVE
EEEECCCCHHHHCCCCCCCCCCCCCCCHHHHHCHHHHCCCCCCCCCEEEEECCCEEEEEC
DFPEVNAQRIADGGKPFANPRNAAAGSLRQKNIEDVKKRRLRMISHGIGFTEGFSPASQH
CCCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DAYLALAAWGLPTSPYTEAVTDPEDVVKKVSYWADHRHDALHEMDGLVIKVDDIASQRAL
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHC
GSTSRAPRWAIAYKYPPEEVTTKLLDIQVGVGRTGRVTPFAVMEPVLVAGSTVSMATLHN
CCCCCCCCEEEEEECCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHCCCCEEHHHHCC
QSEVKRKGVLIGDTVVIRKAGEVIPEVLGPVVELRDGTEREYIFPTLCPECGTRLAPAKA
HHHHHHCCEEECCHHEEHHHHHHHHHHHHHHHHHCCCCCCCEECCHHCHHHCCCCCCCCC
DDVDWRCPNMQSCPGQLSTRLTYLAGRGAFDIEALGEKGAEDLIRTGILLDESGLFDLTE
CCCCCCCCCCCCCCHHHHHHHHHEECCCCCCHHHHCCCCHHHHHHHCCEECCCCCCCCCH
DDLLSSNVYTTNAGKVNASGKKLLDNLQKSKQTDLWRVLVALSIRHVGPTAARALAGRYH
HHHHCCCEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
SIQALIDAPLEELSETDGVGTIIAQSFKDWFEVDWHKAIVDKWAAAGVTMEEEVGEVAEQ
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
TLEGLTIVVTGGLEGFTRDSVKEAIISRGGKASGSVSKKTDYVVIGENAGSKATKAEELG
HHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHC
LRILDEAGFVRLLNTGSADE
CEEECCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA