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The map label for this gene is tetA [H]

Identifier: 62390027

GI number: 62390027

Start: 1187293

End: 1188510

Strand: Direct

Name: tetA [H]

Synonym: cg1288

Alternate gene names: 62390027

Gene position: 1187293-1188510 (Clockwise)

Preceding gene: 62390025

Following gene: 62390028

Centisome position: 36.17

GC content: 53.86

Gene sequence:

>1218_bases
ATGTTAACTCAAAAAATAGAATTAGAGGCTAAACCAAAAATCCCAGAGGAGATCTGGGTGCTGGTTGTGGCTGCGTTTAT
TATTGCGCTGGGCTATGGCCTGATTGCGCCGATTTTGCCACAGTTTGTGGTCGGTTTTGATGTAAGTTTTGCAGCTGCCA
GTGCGGTGGTGTCCATCTTTGCGGGCGCCCGGTTGTTGTTTGCGCCGATGTCGGGGAGTTTGATCGATAAGATCGGTTCC
CGTCGTGTGTATCTCACTGGTTTACTCACCGTGGCTATCACCACGGGGCTTGTTGCGTTGGCGCAGGAATACTGGCAGAT
TCTGCTGCTTCGTGGCATCGCAGGTATTGGTTCCACCATGTTTACGGTCTCTGCCATGGGCCTGATCGTGAAGATGGCGC
CGGTGGAGATCCGCGGGCGGTGTTCGTCGGTATATGCCAGTTCGTTCCTGTTTGGCAATATTATTGGCCCGGTTGTGGGT
GCTGCGATGTCCGGTTTGGGCATGCGGTGGCCGTTCGCGATTTATGGTGCTTCCGTTGGCTTAGCTGCACTTGTTGTGTG
GTGGCGGATGCCGAAAACCAACGATTCACTTCGGAAGGCTGATAGCAATAGTGTGCCGGCGTTGCGCTTTGCTGAGGCAA
TTAAGGATTCTGCCTACCGCTCGGCGTTGTTTAGTGCTTTTGCCAATGGTTGGTCGAACTTTGGTGTGCGTGTGGCGGTT
CTTCCACTGTTTGCCGCAGCTGCATTTAGCAATGGCGGAGCTATTGCGGGTTTTGCCATGGCTGCGTTCGCCGCTGGAAA
TGCTTTGTGTCTGCAATTCGCGGGCGATCTCTCAGATCGCATTGGCCGTAAACCGATGATTATTTCCGGGCTGATCGTCA
ATGCAGTGTTCACGGCAATGATCGGATTCGGCACAGAAGTGTGGATCCTGATCACGGTATCTGCGTTGGCAGGTGCTGGT
GCGGGCTTGCTTAATCCAAGTCAGCAGGCGGTGCTCGCTGATGTTATAGATTCCCGCCCCGGCGGAAAAGTCTTAGCGAA
TTTCCAAATGGCGCAGGATTTCGGTGCGATTGTTGGCCCGATTCTCGTAGGCATGATCGCAGAACAGGCAGGCTTCCAAA
TCGGATTCATGCTGTGTGGTGTGATCAGTTTGCTGGCTGCGGTCGCATGGATCTTCGGCCGGGAGACGCTGCCAACGGCG
AAAGTCGAGCAGGTATAA

Upstream 100 bases:

>100_bases
AGAGCACCCAAGCGACATTGTTCGATAATATGTTCTATTATTGGTATTAATACTGGTCAGCTAGATATAAAAGGGGCGCA
GGGCTAGCCTTGGGTGTGAA

Downstream 100 bases:

>100_bases
ATGACGCCAATTGTGGAGTCCAGGGCGTGGAAAGCTCTGGGCGCTTTAAGTGTTGGGCTGTTTCTCACACTGCTTGACCA
ATCGTTGGTGGCTGTCGCGC

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(C) [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MLTQKIELEAKPKIPEEIWVLVVAAFIIALGYGLIAPILPQFVVGFDVSFAAASAVVSIFAGARLLFAPMSGSLIDKIGS
RRVYLTGLLTVAITTGLVALAQEYWQILLLRGIAGIGSTMFTVSAMGLIVKMAPVEIRGRCSSVYASSFLFGNIIGPVVG
AAMSGLGMRWPFAIYGASVGLAALVVWWRMPKTNDSLRKADSNSVPALRFAEAIKDSAYRSALFSAFANGWSNFGVRVAV
LPLFAAAAFSNGGAIAGFAMAAFAAGNALCLQFAGDLSDRIGRKPMIISGLIVNAVFTAMIGFGTEVWILITVSALAGAG
AGLLNPSQQAVLADVIDSRPGGKVLANFQMAQDFGAIVGPILVGMIAEQAGFQIGFMLCGVISLLAAVAWIFGRETLPTA
KVEQV

Sequences:

>Translated_405_residues
MLTQKIELEAKPKIPEEIWVLVVAAFIIALGYGLIAPILPQFVVGFDVSFAAASAVVSIFAGARLLFAPMSGSLIDKIGS
RRVYLTGLLTVAITTGLVALAQEYWQILLLRGIAGIGSTMFTVSAMGLIVKMAPVEIRGRCSSVYASSFLFGNIIGPVVG
AAMSGLGMRWPFAIYGASVGLAALVVWWRMPKTNDSLRKADSNSVPALRFAEAIKDSAYRSALFSAFANGWSNFGVRVAV
LPLFAAAAFSNGGAIAGFAMAAFAAGNALCLQFAGDLSDRIGRKPMIISGLIVNAVFTAMIGFGTEVWILITVSALAGAG
AGLLNPSQQAVLADVIDSRPGGKVLANFQMAQDFGAIVGPILVGMIAEQAGFQIGFMLCGVISLLAAVAWIFGRETLPTA
KVEQV
>Mature_405_residues
MLTQKIELEAKPKIPEEIWVLVVAAFIIALGYGLIAPILPQFVVGFDVSFAAASAVVSIFAGARLLFAPMSGSLIDKIGS
RRVYLTGLLTVAITTGLVALAQEYWQILLLRGIAGIGSTMFTVSAMGLIVKMAPVEIRGRCSSVYASSFLFGNIIGPVVG
AAMSGLGMRWPFAIYGASVGLAALVVWWRMPKTNDSLRKADSNSVPALRFAEAIKDSAYRSALFSAFANGWSNFGVRVAV
LPLFAAAAFSNGGAIAGFAMAAFAAGNALCLQFAGDLSDRIGRKPMIISGLIVNAVFTAMIGFGTEVWILITVSALAGAG
AGLLNPSQQAVLADVIDSRPGGKVLANFQMAQDFGAIVGPILVGMIAEQAGFQIGFMLCGVISLLAAVAWIFGRETLPTA
KVEQV

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1787291, Length=399, Percent_Identity=23.3082706766917, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42309; Mature: 42309

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 383387; 6337373; 6307828; 1517220 [H]